Cocos nucifera water improves metabolic functions in offspring of high fat diet fed Wistar rats

2018 ◽  
Vol 29 (2) ◽  
pp. 185-194
Author(s):  
Olufadekemi T. Kunle-Alabi ◽  
Opeyemi O. Akindele ◽  
Yinusa Raji
Keyword(s):  
High Fat Diet ◽  
Metabolic Diseases ◽  
Serum Insulin ◽  
Cocos Nucifera ◽  
Fasting Blood Glucose ◽  
Wistar Rat ◽  
Distilled Water ◽  
High Fat ◽  
Water Control ◽  
Metabolic Indices

AbstractBackground:Maternal high fat diet has been implicated in the aetiology of metabolic diseases in their offspring. The hypolipidaemic actions ofCocos nuciferawater improve metabolic indices of dams consuming a high fat diet during gestation. This study investigated the effects ofC. nuciferawater on metabolism of offspring of dams exposed to high fat diet during gestation.Methods:Four groups of pregnant Wistar rat dams (n=6) were treated orally from Gestation Day (GD) 1 to GD 21 as follows: standard rodent feed+10 mL/kg distilled water (Control), standard rodent feed+10 mL/kgC. nuciferawater, high fat feed+10 mL/kg distilled water (high fat diet), and high fat feed+10 mL/kgC. nuciferawater (high fat diet+C. nuciferawater). The feeds were givenad libitumand all dams received standard rodent feed after parturition. Fasting blood glucose was measured in offspring before being euthanized on Postnatal Day (PND) 120. Serum insulin, leptin, lipid profile and liver enzymes were measured.Results:Serum total cholesterol (TC), insulin, alanine transaminase (ALT) and alkaline phosphatase levels were significantly increased (p<0.05) in high fat diet offspring compared with controls. Similar changes were not observed in high fat diet+C. nuciferawater offspring.Conclusions:Results suggest that the adverse effects of maternal high fat diet on offspring’s metabolism can be ameliorated byC. nuciferawater.

scholarly journals Extract of Wax Gourd Peel Prevents High-Fat Diet-Induced Hyperlipidemia in C57BL/6 Mice via the Inhibition of the PPARγPathway

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Ming Gu ◽  
Shengjie Fan ◽  
Gaigai Liu ◽  
Lu Guo ◽  
Xiaobo Ding ◽  
...  
Keyword(s):  
Blood Glucose ◽  
High Fat Diet ◽  
Target Genes ◽  
Metabolic Diseases ◽  
Body Weight Gain ◽  
Fasting Blood Glucose ◽  
Peroxisome Proliferator ◽  
Mrna Levels ◽  
High Fat ◽  
Wax Gourd

Wax gourd is a popular vegetable in East Asia. In traditional Chinese medicine, wax gourd peel is used to prevent and treat metabolic diseases such as hyperlipidemia, hyperglycemia, obesity, and cardiovascular disease. However, there is no experimental evidence to support these applications. Here, we examined the effect of the extract of wax gourd peel (EWGP) on metabolic disorders in diet-induced C57BL/6 obese mice. In the preventive experiment, EWGP blocked body weight gain and lowered serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL-c), liver TG and TC contents, and fasting blood glucose in mice fed with a high-fat diet. In the therapeutic study, we induced obesity in the mice and treated with EWGP for two weeks. We found that EWGP treatment reduced serum and liver triglyceride (TG) contents and fasting blood glucose and improved glucose tolerance in the mice. Reporter assay and gene expression analysis showed that EWGP could inhibit peroxisome proliferator-activated receptorγ(PPARγ) transactivities and could decrease mRNA levels of PPARγand its target genes. We also found that HMG-CoA reductase (HMGCR) was downregulated in the mouse liver by EWGP. Our data suggest that EWGP lowers hyperlipidemia of C57BL/6 mice induced by high-fat diet via the inhibition of PPARγand HMGCR signaling.

Sequoyitol ameliorates diabetic nephropathy in diabetic rats induced with a high-fat diet and a low dose of streptozotocin

2014 ◽  
Vol 92 (5) ◽  
pp. 405-417 ◽  
Author(s):  
Xian-Wei Li ◽  
Yan Liu ◽  
Wei Hao ◽  
Jie-Ren Yang
Keyword(s):  
Diabetic Nephropathy ◽  
Blood Glucose ◽  
High Fat Diet ◽  
Low Dose ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
High Fat

Sequoyitol decreases blood glucose, improves glucose intolerance, and enhances insulin signaling in ob/ob mice. The aim of this study was to investigate the effects of sequoyitol on diabetic nephropathy in rats with type 2 diabetes mellitus and the mechanism of action. Diabetic rats, induced with a high-fat diet and a low dose of streptozotocin, and were administered sequoyitol (12.5, 25.0, and 50.0 mg·(kg body mass)−1·d−1) for 6 weeks. The levels of fasting blood glucose (FBG), serum insulin, blood urea nitrogen (BUN), and serum creatinine (SCr) were measured. The expression levels of p22phox, p47phox, NF-κB, and TGF-β1 were measured using immunohistochemisty, real-time PCR, and (or) Western blot. The total antioxidative capacity (T-AOC), as well as the levels of malondialdehyde (MDA) and reactive oxygen species (ROS) were also determined. The results showed that sequoyitol significantly decreased FBG, BUN, and SCr levels, and increased the insulin levels in diabetic rats. The level of T-AOC was significantly increased, while ROS and MDA levels and the expression of p22phox, p47phox, NF-κB, and TGF-β1 were decreased with sequoyitol treatment both in vivo and in vitro. These results suggested that sequoyitol ameliorates the progression of diabetic nephropathy in rats, as induced by a high-fat diet and a low dose of streptozotocin, through its glucose-lowering effects, antioxidant activity, and regulation of TGF-β1 expression.

scholarly journals PROTECTIVE ROLE OF BERBERINE IN AMELIORATING DIABETIC COMPLICATIONS IN STREPTOZOTOCIN-HIGH FAT DIET MODEL IN EXPERIMENTAL ANIMALS

2020 ◽  
pp. 41-48
Author(s):  
DEEPTI D. BANDAWANE ◽  
SHRUTI B. MOOLIYA ◽  
SHAILAJA B. JADHAV
Keyword(s):  
Diabetic Nephropathy ◽  
Blood Urea Nitrogen ◽  
Diabetic Complications ◽  
High Fat Diet ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Protective Role ◽  
Left Ventricular ◽  
High Fat ◽  
Urea Nitrogen

Objective: Diabetes mellitus is a serious, complex metabolic disorder and growing health threat disease in the world. Berberine, one of the main constituent in Rhizoma coptidis is widely used in the treatment of diabetes. Potential of berberine in the management of diabetic complications, namely diabetic nephropathy and cardiomyopathy, is however, not yet explored. The present study was, therefore, undertaken to explore the potential of berberine for the management of diabetic nephropathy and diabetic cardiomyopathy in high-fat diet (HFD) and low dose streptozotocin (STZ) induced diabetes in rats. Methods: Rats were fed a high-fat diet for 4 w followed by a single intraperitoneal dose of streptozotocin (35 mg/kg). Animals were divided in five groups. Berberine was given orally in two different dose levels (75 mg/kg and 150 mg/kg) for 28 d. Metformin (100 mg/kg) was used as a standard antidiabetic drug. At the end of the study, parameters evaluated includes glycemic profile, lipid profile, left ventricular indices, urinary protein, serum creatinine, blood urea nitrogen and cardiac antioxidants. Histopathology of kidney and pancreas was carried out. Results: Berberine treated groups showed a significant decrease in fasting blood glucose, glycosylated Hb, creatinine, blood urea nitrogen and urinary total proteins, whereas there was a significant improvement in serum insulin, liver glycogen, skeletal muscle glycogen and cardiac antioxidant enzymes. Conclusion: Present study indicated that berberine shows a protective role in diabetes-associated renal and cardiovascular complications.

Abstract P257: High Fat Diet Induces Metabolic Syndrome-Like Phenotype Associated with Adverse Micro- and Macro-Vascular Remodeling

Hypertension ◽  
2015 ◽  
Vol 66 (suppl_1) ◽  
Author(s):  
Aaron J Trask
Keyword(s):  
Metabolic Syndrome ◽  
Blood Glucose ◽  
High Fat Diet ◽  
Vascular Remodeling ◽  
Coronary Flow ◽  
Metabolic Diseases ◽  
Fasting Blood Glucose ◽  
High Fat ◽  
Flow Reserve ◽  
Vascular Bed

Metabolic diseases such as type 2 diabetes (T2DM), hypertension, and metabolic syndrome (MetS) have been associated with vascular disease, and we have previously demonstrated vascular-bed-specific remodeling in both mouse and porcine models of T2DM and MetS. The aim of this study was to determine whether high-fat diet would induce MetS-associated adverse micro- and macro-vascular remodeling and mechanics in mice. Three week old male C57BL/6J mouse siblings were randomized to receive either a normal low-fat (LFD: 10% fat) or high-fat (HFD: 60% fat) diet for 20 weeks (n=7-10 per group). HFD induced a MetS-like phenotype characterized by increased body weight (LFD: 28.6±0.7 vs. HFD: 43.4±0.8 g, p <0.0001), increased mean arterial pressure (LFD: 65±3 vs. HFD: 91±2 mmHg, p <0.0001), increased plasma insulin (LFD: 106±39 vs. HFD: 368±54 pg/mL, p <0.001), and transient increases in fasting blood glucose. Passive pressure myography of septal coronary resistance microvessels (CRMs) revealed reduced internal (LFD: 151±11 vs. HFD: 113±7 μm at 125 mmHg, p <0.05) and external diameters, increased wall/lumen ratio (LFD: 5.5±0.6 vs. HFD: 7.8±0.5 at 125 mmHg, p <0.01) and reduced incremental modulus of elasticity (LFD: 7.9x10 6 ±1.7x10 6 vs. HFD: 4.5x10 6 ±0.6x10 6 dynes/cm 2 at 125 mmHg, p <0.01) in mice fed HFD. Adverse CRM remodeling was associated with reduced coronary flow at baseline and under hyperemic conditions, which reduced coronary flow reserve (LFD: 7.3±0.5 vs. HFD: 5.5±0.5, p <0.05). Aortic pulse wave velocity was increased (LFD: 0.31±0.02 vs. HFD: 0.36±0.01 cm/ms, p <0.05) and significantly correlated with the increased blood pressure (r=0.67, p<0.01). These data demonstrate that 20 weeks of a high-fat diet induces an early MetS-like pathophysiological state that is associated with vascular-bed-specific remodeling and alterations in vascular biomechanics. Furthermore, the presence of adverse vascular remodeling in the presence of an early MetS-like phenotype, but not overt MetS (i.e. in the presence of sustained elevation in fasting blood glucose), may suggest the presence of underlying sub-clinical disease during the early progression of metabolic syndrome.

scholarly journals Kaempferide Improves Glycolipid Metabolism Disorder by Activating PPARγ in High-Fat-Diet-Fed Mice

2020 ◽  
Author(s):  
Qingfu Zeng ◽  
Heng Tang ◽  
Ting Tang ◽  
Peng Pu
Keyword(s):  
Signaling Pathway ◽  
High Fat Diet ◽  
Metabolic Diseases ◽  
Serum Insulin ◽  
High Fat ◽  
Glycolipid Metabolism ◽  
Metabolism Disorder ◽  
Pparγ Agonist ◽  
Underlying Mechanisms

Abstract Background: Kaempferide (Ka, 3,5,7-trihydroxy-4′-methoxyflavone), an active ingredient of Tagetes erecta L has been demonstrated to possess many pharmacological effects, including antioxidant, anti-inflammation, anticancer and antihypertension in previous study. However, there is no evidence of Ka on metabolic disorder in former studies. This study investigated the effects of Ka on glycolipid metabolism and explored the underlying mechanisms of action in vivo and vitro. Methods: High-fat diet (HFD) was used to induce the model of glycolipid metabolism disorder in mice.The hypolipidemic and hypoglycemic effect was detected by several indicators, like blood sample analysis blood glucose, serum insulin, HOMA index and intraperitoneal glucose tolerance tests (IPGTT). The signaling pathways of lipid metabolism (PPARγ/LXRα/ABCA1) and glucose metabolism (PPARγ/PI3K/AKT) were evaluated using Real-Time PCR and Western blot. The primary culture of hepatocytes was prepared to confirm the target of Ka by co-culturing with PPARγ agonist or inhibitor.Results: Administration of Ka at a dose of 10mg/kg for 16 weeks effectively attenuated obesity, hyperlipidemia, hyperglycemia and insulin resistance in HFD mice. Further studies revealed the hypolipidemic and hypoglycemic effects of Ka depended on the activation of PPARγ/LXRα/ABCA1 pathway and PPARγ/PI3K/AKT pathway, respectively. The primary hepatocyte test, co-cultured with PPARγ agonists or inhibitors, further confirmed the above signaling pathway and key protein. Conclusion: Ka played an important role in improving glycolipid metabolism disorder, which were causally associated with weight loss. The underlying mechanisms might are associated with the activation of PPARγ and its downstream signaling pathway. Our study helped to understand the pharmacological actions of Ka, and provides theoretical basis for Ka in the effective treatment of obesity, diabetes and other metabolic diseases.

scholarly journals Cardioprotective and renoprotective effects of Cocos nucifera water in offspring of high fat diet fed Wistar rat dams

2016 ◽  
Vol 4 (8) ◽  
pp. 634-638
Author(s):  
Olufadekemi Tolulope Kunle-Alabi ◽  
◽  
Opeyemi Oreofe Akindele ◽  
Yinusa Raji ◽  
◽  
...  
Keyword(s):  
High Fat Diet ◽  
Cocos Nucifera ◽  
Wistar Rat ◽  
High Fat

scholarly journals Chronic High Fat Diet Intake Impairs Hepatic Metabolic Parameters in Ovariectomized Sirt3 KO Mice

2021 ◽  
Vol 22 (8) ◽  
pp. 4277
Author(s):  
Marija Pinterić ◽  
Iva I. Podgorski ◽  
Marijana Popović Hadžija ◽  
Ivana Tartaro Bujak ◽  
Ana Tadijan ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Metabolic Diseases ◽  
Body Weight Gain ◽  
Lipid Hydroperoxide ◽  
Metabolic Stress ◽  
Lipid Hydroperoxides ◽  
Ros Scavenging ◽  
High Fat ◽  
Preclinical Research ◽  
Female Mice

High fat diet (HFD) is an important factor in the development of metabolic diseases, with liver as metabolic center being highly exposed to its influence. However, the effect of HFD-induced metabolic stress with respect to ovary hormone depletion and sirtuin 3 (Sirt3) is not clear. Here we investigated the effect of Sirt3 in liver of ovariectomized and sham female mice upon 10 weeks of feeding with standard-fat diet (SFD) or HFD. Liver was examined by Folch, gas chromatography and lipid hydroperoxide analysis, histology and oil red staining, RT-PCR, Western blot, antioxidative enzyme and oxygen consumption analyses. In SFD-fed WT mice, ovariectomy increased Sirt3 and fatty acids synthesis, maintained mitochondrial function, and decreased levels of lipid hydroperoxides. Combination of ovariectomy and Sirt3 depletion reduced pparα, Scd-1 ratio, MUFA proportions, CII-driven respiration, and increased lipid damage. HFD compromised CII-driven respiration and activated peroxisomal ROS scavenging enzyme catalase in sham mice, whereas in combination with ovariectomy and Sirt3 depletion, increased body weight gain, expression of NAFLD- and oxidative stress-inducing genes, and impaired response of antioxidative system. Overall, this study provides evidence that protection against harmful effects of HFD in female mice is attributed to the combined effect of female sex hormones and Sirt3, thus contributing to preclinical research on possible sex-related therapeutic agents for metabolic syndrome and associated diseases.

scholarly journals Dysbiosis of intestinal microbiota in early life aggravates high-fat diet induced dysmetabolism in adult mice

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Z. H. Miao ◽  
W. X. Zhou ◽  
R. Y. Cheng ◽  
H. J. Liang ◽  
F. L. Jiang ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
High Fat Diet ◽  
Early Life ◽  
Fasting Blood Glucose ◽  
Fecal Microbiota ◽  
Long Term Effects ◽  
High Fat ◽  
Host Animal ◽  
Intestinal Microbes ◽  
Lipid Metabolism Disorders

Abstract Background Accumulating evidence have shown that the intestinal microbiota plays an important role in prevention of host obesity and metabolism disorders. Recent studies also demonstrate that early life is the key time for the colonization of intestinal microbes in host. However, there are few studies focusing on possible association between intestinal microbiota in the early life and metabolism in adulthood. Therefore the present study was conducted to examine whether the short term antibiotic and/or probiotic exposure in early life could affect intestinal microbes and their possible long term effects on host metabolism. Results A high-fat diet resulted in glucose and lipid metabolism disorders with higher levels of visceral fat rate, insulin-resistance indices, and leptin. Exposure to ceftriaxone in early life aggravated the negative influences of a high-fat diet on mouse physiology. Orally fed TMC3115 protected mice, especially those who had received treatment throughout the whole study, from damage due to a high-fat diet, such as increases in levels of fasting blood glucose and serum levels of insulin, leptin, and IR indices. Exposure to ceftriaxone during the first 2 weeks of life was linked to dysbiosis of the fecal microbiota with a significant decrease in the species richness and diversity. However, the influence of orally fed ceftriaxone on the fecal microbiota was limited to 12 weeks after the termination of treatment. Of note, at week 12 there were still some differences in the composition of intestinal microbiota between mice provided with high fat diet and antibiotic exposure and those only fed a high fat diet. Conclusions These results indicated that exposure to antibiotics, such as ceftriaxone, in early life may aggravate the negative influences of a high-fat diet on the physiology of the host animal. These results also suggest that the crosstalk between the host and their intestinal microbiota in early life may be more important than that in adulthood, even though the same intestinal microbes are present in adulthood.

Nuciferine improves high-fat diet-induced obesity by reducing intestinal permeability through increasing autophagy and remodeling the gut microbiota

2021 ◽  
Author(s):  
Zhen Shi ◽  
Zhiyuan Fang ◽  
Xinxing Gao ◽  
Hao Yu ◽  
Yiwei Zhu ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Intestinal Permeability ◽  
High Fat Diet ◽  
Metabolic Diseases ◽  
High Fat ◽  
Diet Induced Obesity ◽  
Medicinal Value

Nuciferine (NF) has received extensive attention for its medicinal value in the treatment of metabolic diseases, such as obesity, but the effects of NF on obesity-related intestinal permeability, autophagy and...

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