Piroxicam reduces acute and chronic pain response in type 1 diabetic rats

Abstract Objectives Pain associated with various underlying pathologies is a major cause of morbidity and diminished life quality in diabetic patients. Effective control of pain requires the use of analgesics with the best efficacy and with minimal side effects. Therefore, our aim in this study was to investigate the effects of non-steroidal anti-inflammatory drugs (NSAIDs) on pain in diabetic rats. Methods In this study, we investigated the analgesic effects of drugs belonging to three different classes of NSAIDs in a rat model of diabetes. Four diabetic groups received normal saline, diclofenac, piroxicam and ketorolac, respectively, and four non-diabetic groups received normal saline, diclofenac, piroxicam and ketorolac. Type 1 diabetes was induced in rats by a single injection of streptozotocin (60 mg/kg bw). Formalin (50 µL of 2.5%) nociception assay was used to examine the effect of treatment with diclofenac, piroxicam and ketorolac on acute and chronic pain in healthy and diabetic rats. Results Piroxicam showed significant analgesic effects both in the acute phase of pain (5–10 min after injection of formalin into the left hind paw), and in the chronic phase (20–60 min after formalin injection) in healthy as well as diabetic rats. Diclofenac and ketorolac also reduced pain scores in healthy rats. However, these two drugs failed to diminish pain in diabetic rats. Conclusion Our data point for better efficacy of piroxicam in controlling pain in diabetes.

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Enteroprotective effect of Tsukamurella inchonensis on streptozotocin induced type 1 diabetic rats

Turkish Journal of Biochemistry ◽

10.1515/tjb-2018-0309 ◽

2019 ◽

Vol 44 (5) ◽

pp. 683-691

Author(s):

Mehran Mesgari-Abbasi ◽

Solin Ghaderi ◽

Monire Khordadmehr ◽

Katayoon Nofouzi ◽

Hossein Tayefi-Nasrabadi ◽

...

Keyword(s):

Oral Administration ◽

Antioxidant Defense ◽

Antioxidant Defense System ◽

Serum Glucose ◽

Negative Control ◽

Diabetic Rats ◽

Diabetic Patients ◽

Intestinal Damage ◽

Tissue Samples

Abstract Objectives Enteropathy is one of the most important complications of diabetes mellitus. The present study determined the possible effects of Tsukamurella inchonensis (Ti) on diabetes enteropathy on rat small intestine. Materials and methods A total of 40 rats were divided into four groups of 10. Diabetes was induced by streptozotocin. Oral administration of Ti at dose of 105 and 107 CFU/rat was performed in two groups continuously for 14 days. The third and fourth groups received normal saline as the diabetic and negative control groups, respectively. The blood and intestine tissue samples were taken on 21st day post treatment for biochemical and pathological evaluations. Results Significant differences were found in serum glucose, cholesterol and triglycerides values together with in CAT and SOD activities, MDA level and IL-6 concentration in both Ti treated groups in comparison with the diabetic rats. Moreover, there were severe pathological changes including degeneration of intestinal mucosa, mononuclear cell infiltration, decreasing number of goblet cells and villous length associated with increasing in villous thickness on the diabetic rats which markedly attenuated in both Ti recipient groups. Conclusion In conclusion, it seems that oral administration of Ti may improve intestinal damage in diabetic patients by modulation of intestinal antioxidant defense system.

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The localization of HPV and CMV in the adipose tissues of female diabetic type 1 rats and the possibility of having a role of reactivity of COVID-19 in diabetic subjects as a new medical hypothesis

Advances in Obesity Weight Management & Control ◽

10.15406/aowmc.2020.10.00309 ◽

2020 ◽

Vol 10 (3) ◽

pp. 71-73

Author(s):

Ahed J Alkhatib

Keyword(s):

Adipose Tissue ◽

Synergistic Effects ◽

Diabetic Rats ◽

Diabetic Patients ◽

Obesity And Diabetes ◽

Diabetic Model ◽

Medical Hypothesis ◽

The Impact

Introduction: Diabetes has various impacts on human body. It is thought that diabetes is predisposed by obesity. Obesity may due to several factors including genetically-environmental factors. The recent views that viruses may act as etiology for obesity. Study objectives: The main objectives of the present study were to investigate the possibility that CMV and HPV of having a role in initiating episodes of obesity and diabetes, and to test the hypothesis that co-existence of multi-viruses including corona virus may work synergistically to increase the impact of COVID-19 on diabetic patients. Methodology: In this study, a diabetic model was induced, the localization of HPV and CMV was determined using immunohistochemistry. Results: Study findings showed that both viruses HPV and CMV exist in the adipose tissue of diabetic rats. Both viruses were brown in color. Conclusions: Taken together, both CMV and HPV exist in the adipose tissue of diabetic rats, and this may explain the phenomenon of autoimmunity in diabetes from one side and from another side, we may explain the occurrence of synergistic effects of COVID-19 virus and the other viruses mentioned in this study.

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Investigation on Carbohydrate Counting Method in Type 1 Diabetic Patients

BioMed Research International ◽

10.1155/2014/176564 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Osman Son ◽

Belgin Efe ◽

Nazan Erenoğlu Son ◽

Aysen Akalin ◽

Nur Kebapçi

Keyword(s):

Insulin Treatment ◽

Life Quality ◽

The Body ◽

Diabetic Patients ◽

Counting Method ◽

Intensive Insulin Treatment ◽

Diabetic Diet ◽

Count Method ◽

Type 1 Dm

Objective. The results from Diabetes Control and Complications Trial (DCCT) have propounded the importance of the approach of treatment by medical nutrition when treating diabetes mellitus (DM). During this study, we tried to inquire carbohydrate (Kh) count method’s positive effects on the type 1 DM treatment’s success as well as on the life quality of the patients.Methods. 22 of 37 type 1 DM patients who applied to Eskişehir Osmangazi University, Faculty of Medicine Hospital, Department of Endocrinology and Metabolism, had been treated by Kh count method and 15 of them are treated by multiple dosage intensive insulin treatment with applying standard diabetic diet as a control group and both of groups were under close follow-up for 6 months. Required approval was taken from the Ethical Committee of Eskişehir Osmangazi University, Medical Faculty, as well as informed consent from the patients. The body weight of patients who are treated by carbohydrate count method and multiple dosage intensive insulin treatment during the study beginning and after 6-month term, body mass index, and body compositions are analyzed. A short life quality and medical research survey applied. At statistical analysis,t-test, chi-squared test, and Mann-WhitneyUtest were used.Results. There had been no significant change determined at glycemic control indicators between the Kh counting group and the standard diabetic diet and multiple dosage insulin treatment group in our study.Conclusion. As a result, Kh counting method which offers a flexible nutrition plan to diabetic individuals is a functional method.

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Antidiabetic and protective effects of Scrophularia striata ethanolic extract on diabetic nephropathy via suppression of RAGE and S100A8 expression in kidney tissues of streptozotocin-induced diabetic rats

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2019-0186 ◽

2020 ◽

Vol 31 (2) ◽

Author(s):

Mona Alaee ◽

Ahmad Akbari ◽

Hadi Karami ◽

Zahra Salemi ◽

Jamal Amri ◽

...

Keyword(s):

Oxidative Stress ◽

Normal Saline ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Diabetic Rats ◽

Oxidative Stress Markers ◽

Gene Expressions ◽

Stress Markers ◽

Antidiabetic Effects

AbstractBackgroundThe present study was conducted to examine the antidiabetic effects of Scrophularia striata ethanolic extract and to evaluate its effects on oxidative stress markers and RAGE and S100A8 gene expressions in the kidney of type 1 diabetic rats.MethodsA total of 36 rats (weight 200–250 g) were randomly assigned into six groups as follows: Cnt, Cnt + S. striata 100, and Cnt + S. striata 200 that received normal saline, 100 mg/kg bw, and 200 mg/kg bw of ethanol extract of S. striata, respectively; and group Dibt, Dibt + S. striata 100, and Dibt + S. striata 200 that received normal saline, 100 mg/kg bw, and 200 mg/kg bw of ethanol extract of S. striata, respectively. Type 1 diabetes was induced in rats by a single injection of streptozotocin (55 mg/kg bw). After 60 days of treatment, biochemical factors and oxidative stress markers (superoxide dismutase [SOD] and malondialdehyde [MDA]) were measured using spectrophotometric methods. RAGE and S100A8 gene expressions were analyzed using real-time polymerase chain reaction.ResultsDiabetes significantly impairs serum and urine fasting blood glucose (FBG), lipid profile, creatinine, urea, and albumin parameters. After the treatment with S. striata extract, these parameters are close to the normal range. It was shown that the S. striata extract significantly decreased the kidney expression levels of RAGE and S100A8 genes and improved oxidative stress markers (SOD and MDA) in the kidney tissues when compared with the diabetic control group. It was also found that the beneficial effects of the S. striata were dose dependent.ConclusionsThe ethanolic extract of S. striata has beneficial antidiabetic effects. Moreover, by reducing RAGE and S100A8 gene expressions and by improving oxidative stress, S. striata might be used as adjuvant treatment for diabetic complications.

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Long-Term Type 1 Diabetes Enhances In-Stent Restenosis after Aortic Stenting in Diabetes-Prone BB Rats

Journal of Biomedicine and Biotechnology ◽

10.1155/2011/396734 ◽

2011 ◽

Vol 2011 ◽

pp. 1-8

Author(s):

Geanina Onuta ◽

Hendrik C. Groenewegen ◽

Flip A. Klatter ◽

Mark Walther Boer ◽

Maaike Goris ◽

...

Keyword(s):

Autoimmune Diabetes ◽

Diabetic Rats ◽

Diabetic Patients ◽

Pathogenetic Mechanisms ◽

Therapeutic Modalities ◽

Stent Restenosis ◽

Increased Risk ◽

In Stent Restenosis

Type 1 diabetic patients have increased risk of developing in-stent restenosis following endovascular stenting. Underlying pathogenetic mechanisms are not fully understood partly due to the lack of a relevant animal model to study the effect(s) of long-term autoimmune diabetes on development of in-stent restenosis. We here describe the development of in-stent restenosis in long-term (~7 months) spontaneously diabetic and age-matched, thymectomized, nondiabetic Diabetes Prone BioBreeding (BBDP) rats (n=6-7in each group). Diabetes was suboptimally treated with insulin and was characterized by significant hyperglycaemia, polyuria, proteinuria, and increased HbA1clevels. Stented abdominal aortas were harvested 28 days after stenting. Computerized morphometric analysis revealed significantly increased neointima formation in long-term diabetic rats compared with nondiabetic controls. In conclusion, long-term autoimmune diabetes in BBDP rats enhances in-stent restenosis. This model can be used to study the underlying pathogenetic mechanisms of diabetes-enhanced in-stent restenosis as well as to test new therapeutic modalities.

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