The stability and irritability study of the chitosan–Aloe vera spray gel as wound healing

2021 ◽  
Vol 32 (4) ◽  
pp. 651-656
Author(s):  
Dini Retnowati ◽  
Retno Sari ◽  
Esti Hendradi ◽  
Septiani Septiani
Keyword(s):  
Wound Healing ◽  
Thermal Cycling ◽  
Physical Stability ◽  
Aloe Vera ◽  
Protective Layer ◽  
Test Method ◽  
Stability Test ◽  
Dermal Irritation ◽  
Irritation Test ◽  
Dermal Irritation Test

Abstract Objectives Chitosan is a natural polysaccharide widely used in various clinical applications including regeneration of skin tissue. Aloe vera has properties in healing burns on the skin, anti-inflammatory effect, and leaves a protective layer on the skin after drying so it provides protection to the wound. The spray gel of chitosan–A. vera was developed as a wound healing that has combined of effect of both component and easy to use. The purpose of this study was to determine the physical stability and irritability of chitosan–A. vera spray gel. Methods The spray gel stability test was conducted using thermal cycling and centrifugation methods. The organoleptic, viscosity, and pH of the spray were evaluated. The irritation test was performed by Draize Rabbit Test method. Results Chitosan (0.5%)–A. vera (1%) spray gel characteristics has a weak yellow color, clear, and a strong A. vera odor. The pH of the spray gel was 4.88 ± 0.01; and the viscosity was 36.50 ± 0.23 cps. The result from the chitosan (0.5%)–A. vera (1%) spray gel stability test using thermal cycling method showed a decrease of viscosity, but remained stable when evaluated using centrifugation method. There was no difference in the pH and organoleptic observation from both tests. Based on the scoring and analysis of the reaction in rabbit skin, the Primary Irritation Index (PII) obtained was 0.56. Conclusions The spray gel of chitosan (0.5%)–A. vera (1%) was stable and according to response category from the acute dermal irritation test, it can be concluded that chitosan (0.5%)–A. vera (1%) spray gel had a slightly irritating effect.

scholarly journals Characteristics and Stability of Nanostructured Lipid Carrier (NLC) Aleurites Moluccana Seed Oil (AMS oil) Using Various Combinations of Beeswax and Oleum Cacao

2019 ◽  
Vol 9 (01) ◽  
pp. 94-97
Author(s):  
Tristiana Erawati M ◽  
Denara Asha Putri ◽  
Arum Sekar Maharani ◽  
Noorma Asha Putri ◽  
Widji Soeratri
Keyword(s):  
Thermal Cycling ◽  
Real Time ◽  
Seed Oil ◽  
Physical Stability ◽  
Extreme Temperature ◽  
Stability Test ◽  
Nanostructured Lipid Carrier ◽  
Thermal Cycling Test ◽  
Cycling Test ◽  
Aleurites Moluccana

This study aims to determine the effect of the combination of beeswax and oleum cacao on the characteristics and stability of nanostructured lipid carrier-aleurites moluccana seed oil (NLC-AMs oil). The combination ratio of beeswax-oleum cacao and AMs oil is 3:1 which total lipid 20%, while the ratio of beeswax-oleum cacao used were F1(100:0); F2 (50:50); F3 (25:75); and F4 (0:100). These preparations are made by the high shear homogenization method because the processing technique is relatively easier, faster and it is possible to get nanoparticle size. Then characterization and physical stability test (real time, thermal cycling test, and centrifugation) were carried out. The results of this study can be concluded that: 1) NLC-AMs oil with combinations of beeswax and oleum cacao has smaller particle size than those using only single solid lipids (oleum cacao or beeswax). 2) Increased concentration of oleum cacao in the NLC-AMs oil system increases its viscosity. 3) The ratio of beeswax and oleum cacao affects the recrystallization index of the NLC-AMs oil. The lowest recrystallization index is in the NLC-AMs oil with a combination of beeswax-oleum cacao in F2 (50:50). 4) The results of the real time stability test for 8 weeks of storage revealed the NLC-AMs oil system with a combination of solid fat beeswax and oleum cacao on F3 (25:75) had the best stability. 5) All formulas are not stable against extreme temperature changes (thermal cycling test) and shocks (centrifugation) indicated by system separation.

scholarly journals PHYSICAL STABILITY TEST AND ETHANOL CREAM IRRITATION TEST OF Moringa oleifera L.

2021 ◽  
Vol 6 (2) ◽  
pp. 186-195
Author(s):  
Nining Sugihartini ◽  
◽  
Zainab Zainab ◽  
Aji Pamungkas ◽  
◽  
...  
Keyword(s):  
Leaf Extract ◽  
Mechanical Test ◽  
Physical Stability ◽  
Ethanol Extract ◽  
Mechanical Tests ◽  
Stability Test ◽  
Irritation Test ◽  
Topical Dosage ◽  
Moringa Leaf Extract

The development of topical dosage forms of Moringa leaf extract cream has been carried out because of its properties that can moisturize the skin. A pharamceutical preparation must meet the requirements of stability and non toxic. The study aimed to determine the effect of variations un the concentration ethanol extract cream of Moringa leaf of physical stability and irritability. Ethanol extract of Moringa leaves was obtained by maceration method with 50% ethanol solvent. The extract was then formulated in the form of O/W base scarring with concentrations of 1% (F1), 3% (F2), and 5% (F3). Creams were evaluated for physical stability including mechanical tests (centrifugation) and physical stability at room temperature (25 ± 2oC) with parameters pH, viscosity on days 1, 7, 14, 21 and 28. In addition, creams were also evaluated for their irritability in vivo with using test animals (rabbits). The data obtained were analyzed using one-way ANOVA test. The results of the physical stability test showed that an increase in the concentration of Moringa leaf extract caused an increase in viscosity (P <0.5) and a decrease in pH (P <0.5) but did not affect physical stability (mechanical test) and its irritating effect. F2 (1%) has a pH of 7.61 while F4 (5%) is 7.01. Based on the results of the study, it is concluded that variations in the concentration of ethanol extract of Moringa leaves can affect the physical stability of the cream and do not affect its irritation properties. Moringa leaf ethanol extract cream with a concentration of 5% (F4) has physical stability and does not irritate the skin better than other formulas.

scholarly journals USING HAIR GROWTH ACTIVITY, PHYSICAL STABILITY, AND SAFETY TESTS TO STUDY HAIR TONICS CONTAINING ETHANOL EXTRACT OF LICORICE (GLYCYRRHIZA GLABRA LINN.)

2017 ◽  
Vol 9 ◽  
pp. 44
Author(s):  
Sheila Meitania Utami ◽  
Joshita Djajadisastra ◽  
Fadlina Chany Saputri
Keyword(s):  
Hair Growth ◽  
Skin Irritation ◽  
Physical Stability ◽  
Chorioallantoic Membrane ◽  
Ethanol Extract ◽  
Stability Test ◽  
Irritation Test ◽  
Licorice Extract ◽  
Growth Activity ◽  
Skin Irritation Test

Objective: The purpose of this study was to determine the safety, physical stability, and hair growth activity of ethanol extract of licorice.Methods: In this study, 2.5%, 5%, and 10% licorice extract was formulated into a hair tonic as a tonic is easier to use and is not sticky like a semisoliddosage. The hair growth activity test was conducted by rubbing the hair tonic preparations on rabbit’s backs; subsequently, the hair length, hairthickness, hair weight, and hair density were measured. Quantitative analysis of glycyrrhizic acid from the licorice ethanol extract with a ultravioletspectrophotometer showed a level of about 156.65 mg/g or 15.665%. The physical stability test was performed on samples of the tonic stored at low(4±2°C), room (25±2°C), and high (40±2°C) temperature, and a cycling test was also performed. The safety test was performed using an eye irritationtest that employed the Hen’s egg test–chorioallantoic membrane (HET-CAM) method and a skin irritation test that employed the patch test method.Results: The hair tonics containing 5% and 10% licorice extract had an equivalent activity of hair growth and even better than the positive controlcontaining 2% minoxidil. The physical stability test showed that the licorice extract hair tonic has good physical stability. The results of the safety testshowed no skin irritation, whereas the HET-CAM test showed that the hair tonic containing licorice extract showed mild eye irritation.Conclusions: Licorice ethanol extract hair tonic solutions in concentrations of 2.5%, 5%, and 10% had hair growth activity similar to that of thepositive control (minoxidil). They have a good physical and chemical stability, also safe for topical use, except the 2.5% licorice ethanol extract hairtonic solution which caused mild eye irritation.

scholarly journals Formulasi Sediaan Lipbalm dari Ekstrak Biji Kopi Arabika (Coffea Arabica L.) Java Preanger Sebagai Emolien

2021 ◽  
Vol 4 (1) ◽  
pp. 105-111
Author(s):  
Retty Handayani
Keyword(s):  
Coffea Arabica ◽  
Statistical Tests ◽  
Physical Stability ◽  
Test Method ◽  
Coffee Bean ◽  
Arabica Coffee ◽  
Paired Samples ◽  
Coffee Beans ◽  
Irritation Test ◽  
Coffea Arabica L

Lipbalm preparations are a decorative cosmetic dosage form for moisturizing lips. One of the most important components of lipbalm preparations is an emollient. Emollients work by covering the damaged skin with beads of fat so that the skin of the lips becomes softer and moisturized. Arabica coffee beans (Coffea arabica L.) java preanger contain linoleic acid which functions as an emollient. This study aims to make a lipbalm formulation containing extracts of Arabica coffee beans (Coffea Arabica L). Lipbalm preparations made with variations in the concentration of coffee extract 3%, 6% and 9%. The resulting formula is then evaluated for its physical stability. The irritation test is carried out using the patch test method, testing the effectiveness of emollients using a skin analyzer. The results showed that variations in the extract of coffee used (3%, 6% and 9%) showed that the concentration of 9% was more stable. The irritation test results show that all three formulas are safe to use. Based on the results of the effectiveness test shows that the formula with a concentration of 9% gives a good emollient effect after use based on statistical tests the paired samples T-test method.Keywords : emollients, coffee bean extracts, formulation, lipbalm.

Evaluation of the Resistance of Individual Si Die to Cracking

1998 ◽  
Author(s):  
R. Berriche ◽  
R.K. Lowry ◽  
M.I. Rosenfield
Keyword(s):  
Thermal Cycling ◽  
Hardness Test ◽  
Mechanical Stresses ◽  
Test Method ◽  
Processing Conditions ◽  
Micro Hardness ◽  
Test Methodology ◽  
Vickers Micro Hardness

Abstract The present work investigated the use of the Vickers micro-hardness test method to determine the resistance of individual die to cracking. The results are used as an indicator of resistance to failure under the thermal and mechanical stresses of packaging and subsequent thermal cycling. Indentation measurements on die back surfaces are used to determine how changes in wafer backside processing conditions affect cracks that form around impressions produced at different loads. Test methodology and results obtained at different processing conditions are discussed.

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