scholarly journals Assessment of dynamic thiol/disulfide homeostasis in patients with asthma

2018 ◽  
Vol 42 (3) ◽  
pp. 99-104
Author(s):  
Rukiye Nar ◽  
Aliye Gamze Çalış
Keyword(s):  
Oxidative Stress ◽  
Forced Expiratory Volume ◽  
Control Group ◽  
Reactive Protein ◽  
Total Thiol ◽  
Stress Markers ◽  
Group A ◽  
And Gender ◽  
And Control

Abstract Background: Asthma is a chronic inflammatory lung disease and oxidative stress is an important component in airway inflammation. This study aims to investigate dynamic thiol/disulfide homeostasis in patients with asthma. Methods: A total of 103 subjects, including 56 patients with asthma and 47 healthy controls, of similar age and gender were included in the study. The native thiol, total thiol and disulfide levels and the disulfide-native thiol, disulfide-total thiol and native thiol-total thiol ratios were analyzed and compared between the asthma and control groups using a novel automatized spectrophotometric assay. Results: The levels of native thiol (p<0.001), total thiol (p<0.001) and disulfide (p<0.001) were significantly lower and the C-reactive protein (CRP) levels (p<0.001) were significantly higher in patients with asthma when compared with those in the control group. A negative correlation was detected between CRP levels and native thiol, total thiol and disulfide levels (p<0.05). A significant positive correlation was detected between forced expiratory volume in 1 s (FEV1) and forced vital capacity (FVC) levels and native thiol and total thiol levels (p<0.01). Conclusions: The thiol/disulfide homeostasis parameters may be used as novel oxidative stress markers in asthma but further studies are needed to investigate the role of thiol/disulfide homeostasis in asthma.

scholarly journals A Novel Oxidative Stress Mediator in Acute Appendicitis: Thiol/Disulphide Homeostasis

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Sefa Ozyazici ◽  
Faruk Karateke ◽  
Umit Turan ◽  
Adnan Kuvvetli ◽  
Huseyin Kilavuz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Acute Appendicitis ◽  
Perforated Appendicitis ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Reactive Protein ◽  
Total Thiol ◽  
Stress Marker ◽  
Stress Mediator

Aim. To investigate the role of a novel oxidative stress marker, thiol/disulphide homeostasis, in patients diagnosed with acute appendicitis (AA).Methods. In this study, seventy-one (43 male and 28 female) patients diagnosed with AA and 71 (30 male and 41 female) healthy volunteers were included. Age, gender, body mass index (BMI), haemoglobin (Hb), white blood cell (WBC), c-reactive protein (CRP), and thiol/disulphide homeostasis parameters (native thiol, total thiol, disulphide, disulphide/native thiol, native thiol/total thiol, and disulphide/total thiol ratios) were compared between the groups. Thiol/disulphide homeostasis was determined by a newly developed method by Erel and Neselioglu.Results. The native thiol, total thiol, and the native thiol/total thiol ratio levels were statistically significantly decreased in the AA compared with the control group (p<0.001). Disulphide level and the ratios of disulphide/native thiol and disulphide/total thiol were higher in the AA group than in the control group (p<0.001). There was a negative correlation of CRP with native thiol, total thiol, and native thiol/total thiol ratio while there was a positive correlation of CRP with disulphide/native thiol and disulphide/total thiol in the AA group. In the stepwise regression model, risk factors as disulphide/native thiol (OR = 1.368;p=0.018) and CRP (OR = 1.635;p=0.003) were determined as predictors of perforated appendicitis compared to the nonperforated group.Conclusion. This is the first study examining the thiol/disulphide homeostasis as a diagnostic aid in AA and establishing thiol/disulphide homeostatis balance shifted towards the disulphide formation due to thiol oxidation. Further studies are needed to optimize the use of this novel oxidative stress marker in AA.

scholarly journals Studying the Role of C-Reactive Protein in Patients with Sleeve Gastrectomy in Baghdad

2021 ◽  
Vol 3 (1) ◽  
pp. 55-60
Author(s):  
Mohammed Abbas Fadil ◽  
Raya Ezat Maroof ◽  
Moayed Abbas Fadil
Keyword(s):  
Sleeve Gastrectomy ◽  
Healthy Person ◽  
Control Group ◽  
C Reactive Protein ◽  
Serum Samples ◽  
Reactive Protein ◽  
Medical City ◽  
Healthy Control ◽  
And Control

Obesity and severe obesity are increasing serious health problems with an epidemic percentage in most countries. In Sleeve gastrectomy, a part of the stomach structure is removed, limiting its capacity by about two to three. A total of thirty blood samples were collected from patients with obesity and the result was compared with healthy person throughout the time from November 2019 to September 2020. Before sleeve gastrectomy and after more than 6 months of sleeve surgery, the sample was collected from the Medical City/Baghdad Teaching Hospital, the withdrawal was again taken at home to have pre and post sleeve gastrectomy, Patient age ranged between [20-46] years for obese patients and healthy control. Then the serum samples were obtained from patients and control group to screen for C-reactive protein by agglutination method. The result of the present study found that the positivity of CRP in pre-operation is higher than that of post-operative with high significance [P<0.005].

Oxidative stress markers in patients treated with typical and atypical antipsychotics

2011 ◽  
Vol 26 (S2) ◽  
pp. 1465-1465
Author(s):  
M. Padurariu ◽  
A. Ciobica ◽  
I. Dobrin ◽  
C. Joacabine ◽  
C. Stefanescu
Keyword(s):  
Oxidative Stress ◽  
Atypical Antipsychotics ◽  
Specific Activity ◽  
Antipsychotic Agents ◽  
Control Group ◽  
Antioxidant Defences ◽  
Stress Markers ◽  
Daily Dose ◽  
Schizophrenic Patients ◽  
And Gender

IntroductionStudies performed in schizophrenia patients have generally suggested the presence of a compromised antioxidant system, but this is not always consistent with specific observed parameters, which on the whole, show evidences of dysregulation. There are also controversies regarding the oxidative stress status in patients treated with typical vs. atypical antipsychotics.AimIn this context, the aim of the present work was to evaluate the specific activity of some peripheral antioxidant defences like superoxide dismutase (SOD) and glutathione peroxidase (GPX) and the level of a lipid peroxidation maker (malondialdehyde-MDA), in schizophrenic patients treated with typical (haloperidol) or atypical (olanzapine, quetiapine and risperidone) antipsychotics, compared with age-matched healthy subjects.MethodsThe subjects of this study (n = 45), consisted of 35 patients who met DSM-IV criteria for schizophrenia and 10 healthy control age and gender-matched subjects. Patients were of paranoid subtype, with duration of illness for at least 5 years. Nine patients were under haloperidol (1–2 mg daily dose) treatment and 26 (8/10/8) patients were under atypical treatment: quetiapine (300 mg daily dose), olanzapine (20 mg daily dose) or risperidone (2–4 mg daily dose), respectively.ResultsWe found a significant decrease in GPX specific activity and also a significant increase of MDA levels in schizophrenic patients, compared to age-matched control group, regardless of their type of treatment. Additionally, an increase in SOD specific activity was observed, mainly in the patients treated with haloperidol and quetiapine.ConclusionsFurther research is necessary in order to elucidate the effects of different antipsychotic agents on antioxidant enzymes.

scholarly journals Comparison of Oxidant-Antioxidant Status in Patients with Vitiligo and Healthy Population

2015 ◽  
Vol 12 (2) ◽  
pp. 132-136 ◽  
Author(s):  
S Agrawal ◽  
A Kumar ◽  
TK Dhali ◽  
SK Majhi
Keyword(s):  
Oxidative Stress ◽  
Free Radicals ◽  
Vitamin E ◽  
Vitamin C ◽  
Control Group ◽  
Healthy Population ◽  
Significant Difference ◽  
Destruction Of Melanocytes ◽  
And Control

Background Vitiligo is a well-recognized pigmentary disorder of the skin and /or mucous membrane characterized by circumscribed ivory or chalky white macules devoid of identifiable melanocytes. The pathogenesis of vitiligo is complex and still not well understood. According to autocytotoxic hypothesis, oxidative stress has been suggested to be the initial pathogenic event in melanocyte degeneration. The role of free radicals and oxidative damage in the pathophysiology of vitiligo has been documented in recent studies.Objective To evaluate the role of oxidative stress in patients with vitiligo and of healthy controls by measuring levels of the oxidant malondialdehyde (MDA) and antioxidants vitamin C and vitamin E in serum and catalase (CAT) in erythrocytes.Method A total of 80 clinically diagnosed cases of vitiligo and 80 control subjects were included in the study to assess the activity of MDA, vitamin C and vitamin E in serum and CAT in erythrocytes of patients and controls by using the spectrophotometric assay.Result There was statistically significant increase in the levels of MDA in patients with vitiligo compared to the control group (p<0.001). No significant difference was found in the levels of vitamin C (p=0.411) and vitamin E (p=0.771) between the patients with vitiligo and control group. The levels of CAT in the vitiligo patients were found to be significantly lower than those of controls (p<0.001).Conclusion Increased oxidative stress and decreased catalase have been observed in vitiligo patients and the data suggesting that the free radicals may be involved in the destruction of melanocytes or dysregulation of melanogenesis.Kathmandu University Medical Journal Vol.12(2) 2014: 132-136

scholarly journals The Role of Oxidative Stress in the Etiopathogenesis of Gluten-Sensitive Enteropathy Disease

2017 ◽  
Vol 36 (3) ◽  
pp. 243-250 ◽  
Author(s):  
Mustafa Kaplan ◽  
Ihsan Ates ◽  
Mahmut Yüksel ◽  
Yasemin Ozderin Ozin ◽  
Muhammed Yener Akpinar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Paraoxonase 1 ◽  
Stress Index ◽  
Control Group ◽  
Gluten Free ◽  
C Reactive Protein ◽  
Serum Samples ◽  
Reactive Protein ◽  
Gluten Sensitive Enteropathy

SummaryBackground:The objective here is to examine the role of overall oxidative stress in the etiopathogenesis of gluten-sensitive enteropathy disease and its relationship with gluten free diet and autoantibodies.Methods:Eighty gluten-sensitive enteropathy patients and 80 control group participants were included in the study. As oxidative stress parameters, we researched total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), paraoxonase-1 and arylesterase parameters in the serum samples of gluten-sensitive enteropathy patients.Results:In comparison to the control group, gluten-sensitive enteropathy patients had lower TAS, paraoxonase-1 and arylesterase levels and gluten-sensitive enteropathy patients had considerable TOS and OSI levels. In contrast, patients who agreed to the gluten free eating routine had a higher OSI proportion and patients who did not conform to the gluten free eating regimen had a lower paraoxonase-1 level. An affirming reciprocation was de tected amidst TOS and OSI proportion and gluten-sensitive enteropathy autoantibodies and C-reactive protein levels and a negative correlation was found between arylesterase level and gluten-sensitive enteropathy autoantibodies.Conclusions:We observed oxidative stress levels to be higher in gluten-sensitive enteropathy patients contrasted with the control group. Oxidative stress level showed differences in gluten-sensitive enteropathy patients depending on gluten diet content and autoantibody positivity. In point of fact, C-reactive protein and gluten-sensitive enteropathy autoantibodies are identified with oxidative anxiety parameters resulting in the possibility that oxidative stress might be successful in the gluten-sensitive enteropathy pathogenesis.

Thiol-disulfide homeostasis and ischemia-modified albumin levels as indicators of oxidative stress in welders’ lung disease

2019 ◽  
Vol 38 (11) ◽  
pp. 1227-1234 ◽  
Author(s):  
M Karataş ◽  
TN Öziş ◽  
M Büyükşekerci ◽  
M Gündüzöz ◽  
OG Özakıncı ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lung Disease ◽  
Control Group ◽  
Welding Fumes ◽  
Ischemia Modified Albumin ◽  
Total Thiol ◽  
Test Parameters ◽  
Function Test ◽  
And Control ◽  
Mixed Exposure

Welders’ lung disease refers to mixed exposure to different kinds of metals and chemicals from welding fumes, which affect all parts of the respiratory tract including airways and parenchyma together. This study aimed to investigate the oxidative status in patients with welders’ lung (PWL) by means of thiol-disulfide homeostasis and ischemia-modified albumin (IMA) levels. The male welder workers diagnosed with welders’ lung disease and healthy individuals were recruited in the study. Plasma levels of disulfide, disulfide/native thiol ratio, disulfide/total thiol ratio, IMA, and catalase (CAT) were determined. Pulmonary function test parameters of both groups were compared. The thiol-disulfide homeostasis parameters of PWL and control group were as follows: disulfide (20.5 ± 6.3 vs. 16.2 ± 3.9 μmol L−1, p < 0.001), disulfide/native thiol (4.36 (1.59) vs. 4.0 (1.64), p = 0.024), and disulfide/total thiol (4.01 (1.34) vs. 3.71 (1.41), p = 0.024). IMA levels in PWL were significantly higher than the control group (1.37 (0.27) mg dL−1 vs. 0.49 (0.61) mg dL−1, p < 0.001), whereas CAT activities were significantly higher in the control group (106.6 (54.5) kU L−1 vs. 78.3 (67.8) kU L−1, p = 0.003). The findings of the present study revealed that oxidative stress plays a key role in the pathogenesis of welders’ lung disease. Plasma thiol-disulfide homeostasis and IMA levels might be indicators of oxidative stress in PWL.

Heat treatment reduces oxidative stress and protects muscle mass during immobilization

2005 ◽  
Vol 289 (1) ◽  
pp. R134-R139 ◽  
Author(s):  
Joshua T. Selsby ◽  
Stephen L. Dodd
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Muscle Mass ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Antioxidant Protection ◽  
Disuse Atrophy ◽  
The Core ◽  
Group A

This study examined the role of heating on oxidative stress and muscle mass in immobilized limbs. Rats were divided into three groups ( n = 9/group): a control group (Con), an immobilized group (Im), and an immobilized and heated group (ImH). Rats were immobilized in the plantarflexed position for 8 days. The core temperature of the ImH group was elevated to 41–41.5°C on alternating days and maintained for 30 min before cooling. On day 8, both heat shock protein 25 (HSP25) and HSP72 were markedly elevated in the ImH compared with the Im group, whereas results in the Im group were not different from Con. Most notably, the ImH group had significantly larger solei compared with the Im group, which were less than those shown in the Con group. Furthermore, immobilization alone caused a significant increase in oxidative damage, and the addition of heating to immobilization significantly reduced oxidative damage. In an effort to further identify the cause of this protective effect, antioxidant enzyme activities were assessed. CuZnSOD was sharply elevated in Im compared ( P < 0.025) with that in the Con and reduced in the ImH group compared with that in the Im group ( P < 0.025). Catalase was elevated 8% ( P < 0.025) in the Im group compared with the Con group and was similar to the ImH group. Glutathione peroxidase, glutathione reductase, and MnSOD did not differ between groups. These data indicate that heating provides protection against oxidative stress and preserves muscle mass during disuse atrophy. These data also suggest that antioxidant protection is not conferred via antioxidant enzymes, and HSPs may play an important role.

Effect of Oral N-Acetylcysteine Treatment on Plasma Inflammatory and Oxidative Stress Markers in Peritoneal Dialysis Patients: A Placebo-Controlled Study

2010 ◽  
Vol 30 (3) ◽  
pp. 336-342 ◽  
Author(s):  
Marcelo M. Nascimento ◽  
Mohamed E. Suliman ◽  
Margarete Silva ◽  
Tiago Chinaglia ◽  
Josiane Marchioro ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Peritoneal Dialysis ◽  
High Sensitivity ◽  
Controlled Study ◽  
Necrosis Factor Alpha ◽  
Reactive Protein ◽  
Stress Markers ◽  
Before And After ◽  
And Gender ◽  
And Oxidative Stress

♦ BackgroundInflammation and oxidative stress (OS) are cardiovascular risk factors in patients with chronic kidney disease. N-acetylcysteine (NAC) is a thiol-containing antioxidant with anti-inflammatory properties and has been shown to reduce the number of cardiovascular events in hemodialysis patients.♦ MethodsThe current study aimed to determine the effect of oral NAC (2 × 600 mg/daily) on plasma levels of inflammatory and OS markers in peritoneal dialysis (PD) patients. We performed a placebo-controlled study over 8 weeks in 30 patients (40% males, age 52 ± 13 years) on regular PD. Before the study was started, the patients were divided into 2 groups of 15 patients matched for age and gender. 22 patients completed the study (12 on NAC, 10 on placebo). Proinflammatory cytokines [high-sensitivity C-reactive protein, interleukin-6 (IL-6), tumor necrosis factor-alpha, and pentraxin 3] and markers of OS (pentosidine, advanced oxidation protein products, homocysteine, glutathione, asymmetric dimethylarginine, and free sulfhydryls) were measured before and after treatment with NAC.♦ ResultsTreatment with NAC for 8 weeks increased mean baseline plasma NAC levels from 2.6 to 24.8 μmol/L ( p = 0.007). This intervention, which caused no side effects, significantly diminished IL-6 levels, from 9.4 (4.5 – 31) to 7.6 (4.9 – 13.5) pg/mL ( p = 0.006), whereas no such changes were observed in the placebo group. NAC treatment did not significantly affect the other inflammatory and OS markers.♦ ConclusionsShort-term oral NAC treatment resulted in reduction of circulating IL-6, suggesting that such treatment could be a useful strategy in blunting the inflammatory response in PD patients.

scholarly journals Mitochondrial function and oxidative stress markers in higher-frequency episodic migraine

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Elena C. Gross ◽  
Niveditha Putananickal ◽  
Anna-Lena Orsini ◽  
Deborah R. Vogt ◽  
Peter S. Sandor ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mitochondrial Function ◽  
Lipid Peroxide ◽  
Control Group ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Potential Biomarker ◽  
Plasma Antioxidant Capacity ◽  
And Oxidative Stress

AbstractIncreasing evidence points towards the role of mitochondrial functioning, energy metabolism, and oxidative stress in migraine. However not all previous research has been conclusive and some mitochondrial function/oxidative stress markers have not yet been examined. To this end, alpha-lipoic acid (ALA), total thiols, total plasma antioxidant capacity (TAC), lipid peroxide (PerOx), oxidised LDL (oxLDL), HbA1c and lactate were determined in the serum of 32 higher frequency episodic migraineurs (5–14 migraine days/ months, 19 with aura, 28 females) in this cross-sectional study. The majority of patients had abnormally low ALA and lactate levels (87.5% and 78.1%, respectively). 46.9% of the patients had abnormally high PerOx values, while for thiols and TAC over one third of patients had abnormally low values (31.2% and 37.5%, respectively). 21.9% of patients had abnormally low HbA1c and none had an HbA1c level above 5.6%. oxLDL was normal in all but one patient. This study provides further evidence for a role of oxidative stress and altered metabolism in migraine pathophysiology, which might represent a suitable therapeutic target. ALA, being too low in almost 90% of patients, might represent a potential biomarker for migraine. Further research is needed to replicate these results, in particular a comparison with a control group.This study is part of the trial registration: ClinicalTrials.gov: NCT03132233, registered on 27.04.2017, https://clinicaltrials.gov/ct2/show/NCT03132233.

scholarly journals Relationships of oxidative stress and systemic inflammation markers depending on the degree and duration of hypertension

2016 ◽  
Vol 7 (2) ◽  
pp. 118-122
Author(s):  
T. Ashcheulova ◽  
N. Gerasimchuk
Keyword(s):  
Oxidative Stress ◽  
Arterial Hypertension ◽  
Systemic Inflammation ◽  
Control Group ◽  
C Reactive Protein ◽  
Multisystem Disease ◽  
Reactive Protein ◽  
And Gender ◽  
Relationship Of ◽  
Healthy Persons

Arterial hypertension (AH) is a heterogenic and multisystem disease. It has been suggested that oxidative stress (OS) and systemic non-specific inflammation may be involved in pathogenesis of cardiovascular pathology including AH. The aim of our study was to characterize the plasma C-reactive protein (CRP) level as a marker of systemic inflammation in relation to OS development (on the base of 8-isoprostane level assessment), depending on duration and degree of AH. We examined 117 persons, of which 102 patients from 30 to 65 years old (average age – 54.7 years) who had previously not been receiving regular antihypertensive therapy had I–III degrees of essential hypertension and 15 healthy persons (average age – 48.7 years). In 34 patients from this group the degree of OS activity was determined by 8-isoprostane level as the main marker of OS. The control group consisted of 10 healthy persons, by age and gender comparable with the study group. Determination of plasmatic CRP levels and the level of 8-isoprostane in the serum was performed by ELISA. The study established an increase of the plasmatic CRP levels in patients with hypertension, and a statistically significant increase of serum 8-isoprostane content in hypertensive patients compared to the control group. When assessing the relationship of 8-isoprostane and CRP content in patients with different degrees of hypertension we found that the strongest positive relationship between their levels was observed in the case of I degree hypertension. This may indicate the role of oxidative stress in the pathogenesis of hypertension as a damaging mechanism which contributes to the activation of immune mechanisms and further progression of the disease. Increased CRP and 8-isoprostane levels confirm the involvement of autoimmune mechanisms and oxidative stress in the pathogenesis of hypertension. The level of C-reactive protein is dependent on the duration of hypertension, while the 8-isoprostane levels – only on degree of hypertension. A raised level of C-reactive protein can be used as an independent marker of systemic inflammation in patients with arterial hypertension.

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