scholarly journals Novel H2S donor proglumide-ADT-OH protects HUVECs from ox-LDL-induced injury through NF-κB and JAK/SATA pathway

Open Medicine ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. 1318-1327
Author(s):  
Xuelan Ou ◽  
Tianqin Xia ◽  
Chunyan Yang ◽  
Chunlei Yu ◽  
Shipeng Zhang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Superoxide Dismutase ◽  
Protective Effect ◽  
Damage Model ◽  
Protective Mechanism ◽  
Physiological Effects ◽  
Tnf Α ◽  
Cell Proliferation Activity ◽  
Proliferation Activity ◽  
Stat Pathway

Abstract As a gaseous mediator, hydrogen sulfide (H2S) has many physiological effects and pathological effects in atherosclerosis. In recent years, many exogenous H2S donors have been synthesized to study atherosclerosis diseases. In this study, proglumide-(5-(4-hydroxyphenyl)-3H-1,2-dithiole-3-thione) (P-A) was synthesized as a H2S donor. The protective effect and mechanism of P-A on HUVEC that was injured by ox-LDL were detected. The HUEVCs were affected by 100 μmol/L P-A for 24 h; the release of H2S was the largest. After 100 μmol/L P-A acted on HUVEC damage model for 12 h, the cell proliferation activity was the best. The results showed that P-A can downregulate the expression of p-NF-кBp65 protein and reduce the amount of TNF-α and IL-6 and promote the formation of IL-10 by inhibiting the NF-кB pathway, and also induce the expression of superoxide dismutase (SOD) to protect HUVEC from ox-LDL injury. P-A can also regulate JAK/STAT pathway to reduce the expression of p-JAK2 protein and reduce the production of IL-6 and TNF-α. P-A has protective effect on HUVEC injured by ox-LDL, and the protective mechanism is related to the regulation of JAK/STAT pathway and NF-кB pathway.

scholarly journals Novel H2S Donor Proglumide-ADT-OH Protects HUVECs From ox-LDL-Induced Injury Through NF-κB and JAK/SATA Pathway

2020 ◽  
Author(s):  
Xuelan Ou ◽  
Chunyan Yang ◽  
Chunlei Yu ◽  
Shipeng Zhang ◽  
Rong Huang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Superoxide Dismutase ◽  
Protective Effect ◽  
Damage Model ◽  
Protective Mechanism ◽  
Physiological Effects ◽  
Tnf Α ◽  
Cell Proliferation Activity ◽  
Proliferation Activity ◽  
Stat Pathway

Abstract Introduction: As a gaseous me dilator, hydrogen sulfide (H2S) has many physiological effects and pathological effects in atherosclerosis. In recent years, many exogenous H2S donors have been synthesized to study atherosclerosis diseases. Methods: Proglumide-(5-(4-Hydroxyphenyl)-3H-1, 2-dithiole-3-thione) (P-A) was synthesized as a H2S donor. The protective effect and mechanism of P-A on HUVEC that injured by ox-LDL was detected. Results: The HUEVCs was affected by 100μmol/L P-A for 24 hours, the release of H2S was the largest. After 100μmol/L P-A acted on HUVEC damage model for 12h, the cell proliferation activity was the best. The results showed that P-A can down regulate the expression of p-NF-кBp65 protein and reduce the amount of TNF-α and IL-6 and promote the formation of IL-10 by inhibiting the NF-кB pathway, and also induce the expression of superoxide dismutase (SOD) to protect HUVEC from ox-LDL injury. P-A can also regulate JAK/STAT pathway to reduce the expression of p-JAK2 protein and reduce the production of IL-6 and TNF-α. Conclusion: P-A has protective effect on HUVEC injured by ox-LDL, and the protective mechanism is related to the regulation of JAK/STAT pathway and NF-кB pathway.

Effect of key enzyme inhibition of glycolysis on synovial fibroblasts in Rheumatoid Arthritis

2021 ◽  
Vol 2 (1) ◽  
pp. 020-026
Author(s):  
Fuxue Meng ◽  
Xiaomai Tao
Keyword(s):  
Rheumatoid Arthritis ◽  
Cell Proliferation ◽  
Citric Acid ◽  
Pyruvate Kinase ◽  
Inflammatory Factors ◽  
Human Rheumatoid Arthritis ◽  
Atp Content ◽  
Tnf Α ◽  
Cell Proliferation Activity ◽  
Proliferation Activity

Objective: To observe effects of glycolysis on human rheumatoid arthritis Fibroblast-like synoviocytes (HFLS-RA) by inhibiting glycolysis. Methods: Hexokinase inhibitor (3-bromopyruvate, 3-BrPa), 6-phosphofructokinase 1 inhibitor citric acid and pyruvate kinase inhibitor shikonin were applied to HFLS-RA respectively. Cell count 8 Kit detects cell proliferation activity, the activity of hexokinase, 6-phosphofructokinase 1, and pyruvate kinase, as well as the cellular glucose, lactate and ATP content were detected by kits, and the ELISA kit detects the expression of cellular inflammatory factors TNF-α and TGF-β. Results: 10 μg/mL 3-BrPa, 160 μg/mL citric acid and 5 μg/mL shikonin significantly inhibited cell proliferation activity (P<0.001); and significantly inhibited HFLS-RA hexokinase and fructose 6-phosphate Kinase 1 and pyruvate kinase activity; Glucose, lactate and ATP content decreased; TNF-α expression decreased, while TGF-β expression increased. Conclusion: This study explored the changes in glucose metabolism and the expression of inflammatory factors in HFLS-RA by inhibiting the key enzymes of glycolysis, further confirming the important role of glycolysis in HFLS-RA, and laying a theoretical basis for a deep understanding of the pathogenesis of RA.

Cyclin D1 expression in astrocytomas is associated with cell proliferation activity and patient prognosis

1999 ◽  
Vol 188 (3) ◽  
pp. 289-293 ◽  
Author(s):  
Satu-Leena Sallinen ◽  
Pauli K. Sallinen ◽  
Juha T. Kononen ◽  
Kirsi M. Syrj�koski ◽  
Nina N. Nupponen ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cyclin D1 ◽  
Cell Proliferation Activity ◽  
Proliferation Activity ◽  
Patient Prognosis

scholarly journals The Activity of Combination of Hydrolyzed Virgin Coconut Oil and Chitosan Toward Wound Healing Parameters on NIH 3T3 Cells Using in Vitro Methods

1970 ◽  
Vol 7 (3) ◽  
pp. 14-19 ◽  
Author(s):  
Hekdin Marsius Sipayung ◽  
Jansen Silalahi ◽  
Yuandani Y
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Protein Expression ◽  
Scratch Wound ◽  
Cell Proliferation Activity ◽  
Proliferation Activity ◽  
Cox 2 ◽  
Nih 3T3 ◽  
Vegf Protein

Objectives: The objective of this study was to investigate the activity of combination of hydrolyzed VCO (HVCO) and chitosan on NIH 3T3 cell proliferation activity, NIH 3T3 cell migration, COX-2 and VEGF protein expression. Design: In vitro cytotoxic assay was determined by MTT (MicrocultureTetrazoliumTehnique) assay, cell proliferation activity was measured by calculating cell viability incubated 24 hours, 48 hours and 72 hours, wound closure percentage was tested by scratch wound healing method, expression of COX-2 protein and VEGF protein were measured by immunocytochemical method. Interventions: The variable that was intervened in this study was the concentration of HVCO and chitosan. Main Outcome Measures: The main measurements carried out in this study were the absorbance value of HVCO and chitosan which was converted into viability cell, proliferation activity, percentage of wound closure, and percentage of COX-2 and VEGF protein expression. Results: Cytotoxic activity of HVCO and chitosan resulted the best concentration at 31.25 μg/ml, scratch wound healing assay from a combination HVCO and chitosan resulted the best migration of fibroblast cells at a ratio of 1:1 with HVCO 62.5 μg/ml and chitosan 62.5 μg/ml, combination of HVCO 62.5 μg/ml and chitosan 62.5 μg/ml (1:1) increased expression of COX-2 and VEGF. Conclusion: Combination of HVCO and chitosan could increase NIH 3T3 cell migration, COX-2 and VEGF protein expression. Combination of HVCO and chitosan had better wound healing activity in vitro than single use. Keywords: Rhizomucor miehei, viability, proliferation, migration, expression

scholarly journals ANTIOXIDANT AND CYTOTOXIC ACTIVITY OF COMBINED EXTRACTS PREPARED USING FICUS RELIGIOSA AND FICUS BENGHALENSIS LEAVES AGAINST CERVICAL CANCER CELL LINE (HELA)

2018 ◽  
Vol 11 (12) ◽  
pp. 407
Author(s):  
Suriya Kumaresan ◽  
Rema Ramasamy ◽  
Philip Robinson Jayachandran
Keyword(s):  
Cervical Cancer ◽  
Hydrogen Peroxide ◽  
Cell Proliferation ◽  
Cytotoxic Activity ◽  
Methanol Extract ◽  
Ficus Religiosa ◽  
Ficus Benghalensis ◽  
Cell Proliferation Activity ◽  
Reduction Assay ◽  
Proliferation Activity

Objectives: Medicinal plants and herbs are used in combination in Ayurveda and folklore medicine as they exhibit good cytotoxic activity. In the present study, the antioxidant, phytochemical, and cell proliferation activity of the combined crude methanolic extract of Ficus religiosa and Ficus benghalensis leaves were investigated.Methods: Antioxidant activity was performed by 2, 2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and hydrogen peroxide methods, and the presence of the phytochemicals was screened using the gas chromatography–mass spectrometry. The extract was further evaluated for its cell proliferation activity against cancer cells using the mitochondrial reduction assay. Antioxidant property of the extracts was measured using the DPPH, hydrogen peroxide, and ferric-reducing antioxidant power assay, respectively, using the UV spectrophotometer.Results: The combined extract exhibited strong antioxidant potential in DPPH assay by increase in the percentage of inhibition with the increase in concentration. Similarly, the IC50 value of the methanol extract in peroxidase scavenging activity was 49.85 μg/mL comparatively lower than the ascorbic acid used as standard. The phytochemical analysis of the methanol extract showed the presence of nine phytoconstituents, which exhibit antioxidant and anticancer property. Mitochondrial reduction assay performed to evaluate the cell proliferation activity of the combined leave extract showed that increase in the concentration of the extract decreased the cell proliferation in the HeLa cell line.Conclusion: The results of present study show a possible synergistic activity of leaves against human cervical cancer.

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