Novel H2S Donor Proglumide-ADT-OH Protects HUVECs From ox-LDL-Induced Injury Through NF-κB and JAK/SATA Pathway
Abstract Introduction: As a gaseous me dilator, hydrogen sulfide (H2S) has many physiological effects and pathological effects in atherosclerosis. In recent years, many exogenous H2S donors have been synthesized to study atherosclerosis diseases. Methods: Proglumide-(5-(4-Hydroxyphenyl)-3H-1, 2-dithiole-3-thione) (P-A) was synthesized as a H2S donor. The protective effect and mechanism of P-A on HUVEC that injured by ox-LDL was detected. Results: The HUEVCs was affected by 100μmol/L P-A for 24 hours, the release of H2S was the largest. After 100μmol/L P-A acted on HUVEC damage model for 12h, the cell proliferation activity was the best. The results showed that P-A can down regulate the expression of p-NF-кBp65 protein and reduce the amount of TNF-α and IL-6 and promote the formation of IL-10 by inhibiting the NF-кB pathway, and also induce the expression of superoxide dismutase (SOD) to protect HUVEC from ox-LDL injury. P-A can also regulate JAK/STAT pathway to reduce the expression of p-JAK2 protein and reduce the production of IL-6 and TNF-α. Conclusion: P-A has protective effect on HUVEC injured by ox-LDL, and the protective mechanism is related to the regulation of JAK/STAT pathway and NF-кB pathway.