scholarly journals Induction of 2n pollen by colchicine in Populus × popularis and its triploids breeding

2011 ◽  
Vol 60 (1-6) ◽  
pp. 155-160 ◽  
Author(s):  
X. J. Xi ◽  
X. B. Jiang ◽  
D. Li ◽  
L. Q. Guo ◽  
J. F. Zhang ◽  
...  
Keyword(s):  
Treatment Process ◽  
Orthogonal Design ◽  
Male Flower ◽  
Colchicine Treatment ◽  
2N Pollen ◽  
Chromosome Counting ◽  
Flower Buds ◽  
Diakinesis Stage ◽  
Populus Popularis ◽  
Different Levels

AbstractInduction of 2n pollen is a required technique for cultivating polyploid via sexual polyploidy. Orthogonal design or Taguchi Design was applied to select the best treatment process of 2n pollen induction inPopulus×popularisfrom different levels of the meiosis stage of male flower buds, colchicine concentration, times of injection, and interval between injections. Flow cytometry and chromosome counting were used to identify the triploids from the offspring ofP.×euramericana. (Dode) Guinier pollinated with induced pollen ofP.×popularis. The results showed that high 2n pollen rate can be achieved by selecting the flower buds during diakinesis stage in meiosis, and then injecting 0.6% colchicine 4 times with 2 hours interval. The 2n pollen rate reached 62.10% by this process, and two triploids were obtained, which indicates that it is possible for cultivating triploids via 2n pollen induction by colchicine treatment in poplar. Results and protocol related to 2n pollen induction, polyploid identification and effect of 2n pollen in this study might be applicable in polyploidy breeding in sectionAigeirosandTacamahacaof poplar.

scholarly journals Colchicine-induced chromosome doubling in Pennisetum interspecific hybrids and its effect on plant morphology

2020 ◽  
Vol 80 (01) ◽  
Author(s):  
Arshpreet Kaur ◽  
Rahul Kapoor ◽  
Yogesh Vikal ◽  
Anu Kalia
Keyword(s):  
Interspecific Hybrids ◽  
Biomass Yield ◽  
Chromosome Doubling ◽  
Plant Morphology ◽  
Colchicine Treatment ◽  
Stem Cuttings ◽  
Chromosome Counting ◽  
Stomatal Frequency ◽  
Preliminary Screening ◽  
Stomata Size

We report the production of hexaploid plants of interspecific hybrids of Pennisetum, with the ultimate aim to improve the biomass yield, drought tolerance and multicut behaviour of this genus. Chromosome doubling was achieved with the application of colchicine at three different concentrations (0.05, 0.1 and 0.2%) for two time durations (12 and 24 hours). The root slips and stem cuttings of interspecific hybrids were used for treatment and the root slips were found to be more efficient. The preliminary screening to select the putative hexaploid plant was done based on stomatal frequency and morphology. Plants containing significantly lower stomatal frequency and larger stomata size were selected for further analysis by chromosome counting. This experiment confirmed that 0.1% concentration of colchicine treatment to root slips for 24 hours was more effective to induce the amphiploids in Pennisetum.

scholarly journals The growth of flower bud, life history, and population structure of Rafflesia arnoldii (Rafflesiaceae) in Bengkulu, Sumatra, Indonesia

2020 ◽  
Vol 21 (2) ◽  
Author(s):  
AGUS SUSATYA
Keyword(s):  
Population Structure ◽  
Life History ◽  
Male Flower ◽  
Female Flower ◽  
Exponential Model ◽  
Flower Buds ◽  
Flower Bud ◽  
Dynamics Of Population ◽  
History Of ◽  
Two Populations

Abstract. Susatya A. 2020. The growth of flower bud, life history, and population structure of Rafflesia arnoldii (Rafflesiaceae) in Bengkulu, Sumatra, Indonesia. Biodiversitas 21: 792-798. The life history of Rafflesia arnoldii R.Br. is the reflection of the complex interaction between flower bud development and the external environments in order to reach its optimal survivorship. The objectives of the study were to determine the growth of flower buds at various development stages, to reconstruct the life history, and to know the population structure of R. arnoldii. The study was carried out at Taba Penanjung, Bengkulu Province, Indonesia. Two populations consisting of 17 individual buds of R. arnoldii were selected for the research. All buds were categorized into six visible stages, mapped, measured their diameters, and recorded their fates every two weeks for six months. The exponential model of growth development was applied to reconstruct the life history. The results showed that buds from the perigone stage respectively grew 3.5 and 12 times faster than those from the bract and cupule stages. The exponential growth of flower bud was confirmed, and explained by Y = 0.785 e0.0052 X, where Y and X were respectively diameter and age of flower bud. The complete life history of R. arnoldii required 3.5 to 5 years, where a female flower needed a longer time than a male flower. The population structure of R. arnoldii was not constant, but changed dynamically over time. The dynamics of population structure was mainly caused by the high mortality of small buds and the low flower bud recruitment.

Ontogeny of floral organs and morphology of floral apex in Phellodendron amurense (Rutaceae)

2002 ◽  
Vol 50 (5) ◽  
pp. 633 ◽  
Author(s):  
Qingyuan Zhou ◽  
Yinzheng Wang ◽  
Xiaobai Jin
Keyword(s):  
Laser Scanning ◽  
Male Flower ◽  
Female Flower ◽  
Laser Scanning Confocal Microscopy ◽  
Morphological Evidence ◽  
Phellodendron Amurense ◽  
Flower Buds ◽  
Floral Organs ◽  
Floral Apex ◽  
Female Flowers

The ontogeny of floral organs and the morphology of floral apex in the dioecious Phellodendron amurense Rupr. were investigated by light microscopy (LM), scanning electron microscopy (SEM) and laser scanning confocal microscopy (LSCM). Investigations indicated that P. amurense is hermaphroditic in its organisation and a common set of floral organs (sepals, petals, stamens and carpels) arise in all flowers during the early stages of development. Later, selective abortion of gynoecium and androecium occurs resulting in dimorphic unisexual flowers. The carpels in male flower buds become different from those in female flower buds soon after their initiation. The stamens of female flowers are not differentiated into anthers and filaments before abortion. The poorly differentiated carpel of male flowers never develops normal structures. Floral morphological evidence supports that Zanthoxylum, Tetradium and Phellodendron are related to one another in a linear sequence. LSCM revealed some interesting features on the apical meristem surface such as zonal differentiation, a triangular or sectorial cell, radiating cell files and linear rows of anticlinal cell walls fluorescing relatively brightly. The concept of carpel-enhancing meristem in the floral apex is tentatively proposed to account for the different fates of carpel development in male and female flowers in P. amurense.

scholarly journals Differential gene expression among three sex types reveals a MALE STERILITY 1 (CpMS1) for sex differentiation in papaya

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Dessireé Zerpa-Catanho ◽  
Jennifer Wai ◽  
Ming Li Wang ◽  
Li’ang Yu ◽  
Julie Nguyen ◽  
...  
Keyword(s):  
Sex Determination ◽  
Male Sterility ◽  
Flower Development ◽  
Sex Differentiation ◽  
Male Flower ◽  
Organ Development ◽  
Flower Buds ◽  
Flower Organ ◽  
Expression Of Genes ◽  
Development Processes

Abstract Background Carica papaya is a trioecious plant species with a genetic sex-determination system defined by sex chromosomes. Under unfavorable environmental conditions male and hermaphrodite exhibit sex-reversal. Previous genomic research revealed few candidate genes for sex differentiation in this species. Nevertheless, more analysis is still needed to identify the mechanism responsible for sex flower organ development in papaya. Results The aim of this study was to identify differentially expressed genes among male, female and hermaphrodite flowers in papaya during early (pre-meiosis) and later (post-meiosis) stages of flower development. RNA-seq was used to evaluate the expression of differentially expressed genes and RT-qPCR was used to verify the results. Putative functions of these genes were analyzed based on their homology with orthologs in other plant species and their expression patterns. We identified a Male Sterility 1 gene (CpMS1) highly up-regulated in male and hermaphrodite flower buds compared to female flower buds, which expresses in small male flower buds (3–8 mm), and that might be playing an important role in male flower organ development due to its homology to MS1 genes previously identified in other plants. This is the first study in which the sex-biased expression of genes related to tapetum development in the anther developmental pathway is being reported in papaya. Besides important transcription factors related to flower organ development and flowering time regulation, we identified differential expression of genes that are known to participate in ABA, ROS and auxin signaling pathways (ABA-8-hydroxylases, AIL5, UPBEAT 1, VAN3-binding protein). Conclusions CpMS1 was expressed in papaya male and hermaphrodite flowers at early stages, suggesting that this gene might participate in male flower organ development processes, nevertheless, this gene cannot be considered a sex-determination gene. Due to its homology with other plant MS1 proteins and its expression pattern, we hypothesize that this gene participates in anther development processes, like tapetum and pollen development, downstream gender specification. Further gene functional characterization studies in papaya are required to confirm this hypothesis. The role of ABA and ROS signaling pathways in papaya flower development needs to be further explored as well.

scholarly journals High frequency 2n pollen formation in black poplar (Populus nigra L.) induced by colchicine

2019 ◽  
Vol 47 (3) ◽  
Author(s):  
Wenting XU ◽  
Yan ZHANG ◽  
Liqin GUO ◽  
Jian ZHAO ◽  
Zunzheng WEI ◽  
...  
Keyword(s):  
High Frequency ◽  
Colchicine Treatment ◽  
Tree Breeding ◽  
Populus Nigra ◽  
Digital Object Identifier ◽  
2N Pollen ◽  
Digital Object ◽  
The World ◽  
Pollen Formation ◽  
First Time

Populus nigra L. is one of the most important male genetic donors in populus genetic improvement and tree breeding over the world. Many excellent Populus nigra clones are identified as triploids potentially obtaining by hybridization of 2n pollen and normal oogamete. This study revealed the cytological mechanism of 2n pollen formation in Populus nigra L. for the first time and concluded the best treated combination of colchicine treatment, which obtained 2n pollen with the highest rate reaching at 87.11% (even 100% 2n pollen in some floral buds) which made the polyploid hybridization utilizing 2n pollen in section Aigeiros possible.   ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

Corrigenda to “Taxonomic revision of Chenopodiaceae in Nepal” [Phytotaxa 191: 10–44. 2014]

Phytotaxa ◽  
2015 ◽  
Vol 226 (3) ◽  
pp. 288 ◽  
Author(s):  
Alexander P. Sukhorukov ◽  
Maria Kushunina
Keyword(s):  
Leaf Anatomy ◽  
Uttar Pradesh ◽  
Male Flower ◽  
Taxonomic Revision ◽  
New Combination ◽  
Eastern Himalaya ◽  
Original Material ◽  
Flower Buds ◽  
Long Time ◽  
Kranz Leaf Anatomy

The first treatment of the family Chenopodiaceae for the flora of Nepal (Central and Eastern Himalaya) has been recently published (Sukhorukov & Kushunina 2014). However, after a detailed investigation of original material concerning Chenopodium pallidum Moquin-Tandon (1840: 30), which is a part of Jacquemont’s collection from India (Herbarium P), we can state that all these specimens indeed belong to Atriplex Linnaeus (1753: 1052). The morphological differences between Atriplex and Chenopodium Linnaeus (1753: 218) are clear in mature plants only, whereas the plants in the type material were gathered in vegetative or early blooming stage (with flower buds only). This explains why the specimens have remained misidentified for such a long time. The characters which support our statement are: (1) Kranz leaf anatomy, which is typical of many Atriplex species (Sukhorukov 2006) placed into the large ‘C4-clade’ (Kadereit et al. 2010), but never observed in Chenopodium, (2) unisexual flowers (only male flower buds were found, because female flowers are absent at early blooming stage) which of all Chenopodieae in its current circumscription are present only in Atriplex (Sukhorukov & Zhang 2013). The “Eastern India” (Fr.: “Indes Orientales” after Jacquemont, 1834), where the plants were collected, applies to the territories of present-day West Bengal, Uttar Pradesh, Delhi, Rajasthan (northern part), Uttarakhand, Himachal Pradesh, Jammu & Kashmir, Punjab (India, Pakistan), and bordering parts of Xizang (China). Only some Atriplex species with Kranz leaf anatomy occur in this region (Zhu et al. 2003, Klimeš & Dickoré 2005, Sukhorukov 2006), such as: A. centralasiatica Iljin (1936: 124), A. pamirica Iljin (1936: 124), and A. schugnanica Iljin (1936: 123). However, the plants known as A. schugnanica are the best match to the Jacquemont’s specimens due to aphyllous or bracteose (not leafy) inflorescence. According to Art. 11 of ICN (McNeill et al. 2012), the name Chenopodium pallidum appears to be an older name at specific rank for Atriplex schugnanica Iljin (1936: 123), and thus a new combination is proposed in the present paper. Besides, new Chenopodium species, previously named Chenopodium pallidum, is described from Nepal.

scholarly journals Male-fertility genes expressed in male flower buds of Silene latifolia include homologs of anther-specific genes

2005 ◽  
Vol 80 (6) ◽  
pp. 403-413 ◽  
Author(s):  
Amr Ageez ◽  
Yusuke Kazama ◽  
Ryuji Sugiyama ◽  
Shigeyuki Kawano
Keyword(s):  
Male Fertility ◽  
Male Flower ◽  
Silene Latifolia ◽  
Flower Buds ◽  
Fertility Genes

scholarly journals Stability in chromosome number and DNA content in synthetic tetraploids of Lolium multiflorum after two generations of selection

2017 ◽  
Vol 47 (2) ◽  
Author(s):  
Roselaine Cristina Pereira ◽  
Natália de Souza Santos ◽  
Fernanda de Oliveira Bustamante ◽  
Andrea Mittelmann ◽  
Vânia Helena Techio
Keyword(s):  
Chromosome Number ◽  
Dna Content ◽  
Genetic Stability ◽  
Chromosome Doubling ◽  
Lolium Multiflorum ◽  
Colchicine Treatment ◽  
Italian Ryegrass ◽  
Chromosome Counting ◽  
Two Generations ◽  
Viable Seeds

ABSTRACT: Chromosome doubling of Italian ryegrass genotypes ( Lolium multiflorum Lam.) adapted to the brazilian edaphoclimatic conditions is an important strategy used by breeders and aims to obtain more vigorous genotypes with better forage quality and disease resistance. The effectiveness of chromosome doubling can be measured by genetic stability and fertility rates of plants over generations. However, a common problem in the polyploidization process is the regeneration of mixoploid plants that have impaired fertility and genetic stability. The objective of this study was to verify if progenies of recently tetraploidized plants remain stable regarding DNA content and chromosome number, over two generations. Progenies of L. multiflorum plants artificially tetraploidized with colchicine treatment were evaluated. Chromosome counting and estimates of the DNA content were used to evaluate the genetic stability. The percentage of tetraploid plants (4X) increased over generations (18%, 34% and 91% in cycle 0, 1 and 2, respectively). All progenies identified as tetraploid by flow citometry showed variation in chromosome number (mixoploidy), but produced viable seeds. Results showed that stabilization in chromosome number and DNA content in tetraploidized plant progenies requires time and that the success of this procedure depends on a continuous and accurate screening and selection.

Use of Ipomoea trifida (HBK.) G. Don germ plasm for sweet potato improvement. 1. Development of synthetic hexaploids of I. trifida by ploidy-level manipulations

Genome ◽  
1991 ◽  
Vol 34 (2) ◽  
pp. 201-208 ◽  
Author(s):  
Masaru Iwanaga ◽  
Rosanna Freyre ◽  
Gisella Orjeda
Keyword(s):  
Sweet Potato ◽  
Ploidy Level ◽  
Ipomoea Batatas ◽  
Colchicine Treatment ◽  
Pollen Production ◽  
2N Pollen ◽  
Ipomoea Trifida ◽  
Pollen Sample ◽  
Colchicine Solution ◽  
Synthetic Hexaploids

Crosses were made between 21 tetraploid accessions and 41 diploid accessions of Ipomoea trifida, obtaining a total of 9185 triploid seeds from 215 different cross combinations. Doubling the somatic chromosome number of the triploids was attempted by colchicine treatment on young seedlings to obtain synthetic hexaploids. A total of 787 axillary buds of 316 triploid plants were treated with a 0.5% colchicine solution for 24 h, applied to cotton plugs surrounding the buds. The survival rate of the treated buds was, on average, 41%. The ploidy level in germ-layer L2 was determined in 258 clones by evaluating a pollen sample from each clone. Fifty-five clones were selected for high stainability and pollen size. The selected genotypes were meiotically analyzed. Twenty-two of them were identified as hexaploid and 33 as triploid with 2n pollen production. The present study is the first report on 2n pollen production in triploid plants of Ipomoea species. The use of these triploid clones with 2n pollen production and synthetic hexaploid clones for sweet-potato breeding is discussed.Key words: Ipomoea batatas, Ipomoea trifida, colchicine, triploids, 2n pollen.

scholarly journals Direct shoot regeneration from male immature flower buds of Musa paradisiaca Linn. cv. Poovan (AAB)

2018 ◽  
Vol 5 (4) ◽  
pp. 142-148
Author(s):  
Anjana R G Nair ◽  
P Ravichandran ◽  
Mathew Bejoy
Keyword(s):  
Shoot Regeneration ◽  
Male Flower ◽  
Basal Region ◽  
Ms Medium ◽  
Direct Shoot Regeneration ◽  
Benzyl Adenine ◽  
Flower Buds ◽  
Flower Bud ◽  
Musa Paradisiaca ◽  
Direct Shoot

A tissue culture system has been developed to multiply Musa paradisiaca cv. Poovan using male immature flower bud and to establish it in ex vitro condition. Size of explants has been found an influencing factor for culture initiation. Immature male flower bud segments of 3 cm size were ideal for better survival and subsequent shoot regeneration. Direct shoot regeneration was achieved from male immature flower buds on Murashige and Skoog (MS) medium supplemented with varying concentrations of plant growth regulators. Initially, actively dividing meristematic region developed at the basal region of flower buds near the bract axil, which later grew into green shoot buds in most of the PGR treatments. Single use of benzyl adenine were found beneficial than kinetin or addition of indole-3-acetic acid. Maximum production of 31.0 ± 0.65 shoots was achieved on MS + 3% sucrose + 6 mg/L benzyl adenine in 15 weeks. Isolated healthy shoots were rooted in half-strength MS medium with 150 mg/L activated charcoal + 30 g/L sucrose + 1 mg/L indole-3-butyric acid within 15 days and they established successfully in greenhouse conditions with 85 % survival.

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