Aerobe Glykolyse in mit Poliomyelitis-Viren und ECHO-Virus Typ 9 infizierten Gewebekulturen

1964 ◽  
Vol 19 (10) ◽  
pp. 899-905 ◽  
Author(s):  
Th. Luthardt ◽  
D. Wilken
Keyword(s):  
Cell Cultures ◽  
Rate Increase ◽  
Aerobic Glycolysis ◽  
Virus Multiplication ◽  
Anaerobic Glycolysis ◽  
Poliomyelitis Virus ◽  
Echo Virus ◽  
Multiplication Cycle ◽  
Cytopathogenic Effect ◽  
Glycolytic Rate

Experiments on aerobic glycolytic metabolism in tissue cultures during the multiplication of poliomyelitis virus led to varying results. Some authors found the rate unaltered, others observed a transitory, but considerable increase.In this series of experiments aerobic glycolysis was measured after infection of monkey kidney and HeLa cell cultures with all three serotypes of poliomyelitis virus and with ECHO-Virus type 9. Virus multiplication was measured and the cytopathogenic effect observed in the same cultures.At the time of inoculation the cell cultures had varying rates of glycolysis, some none at all. In none of the experiments was virus multiplication associated with a change in glycolytic rate. In some cases a transitory rate increase could be observed after the completion of virus liberation. This phenomenon is not a result of virus multiplication but probably of cytolysis. The capability of glycolytic rate increase in the cell-cultures used was proved with 2.4-dinitrophenol.After the end of the virus multiplication cycle the rate of aerobic glycolysis does not drop suddenly, as does that of respiration and anaerobic glycolysis. This finding is considered to show that respiration and anaerobic glycolysis play the essential part in the energy-donating metabolism of cell-cultures.The increase of the glycolytic rate during enterovirus infection described in the literature is nonspecific with respect to virus multiplication.

Clinical significance and the first identification of human parechoviruses in Hungary

2011 ◽  
Vol 152 (25) ◽  
pp. 1007-1012 ◽  
Author(s):  
Gábor Reuter ◽  
Mária Új ◽  
Péter Pankovics ◽  
Tímea Kolozsi ◽  
Ilona Mihály ◽  
...  
Keyword(s):  
Central Europe ◽  
Cell Cultures ◽  
Clinical Importance ◽  
Diagnostic Laboratory ◽  
Fecal Samples ◽  
Reverse Transcription Pcr ◽  
Archived Samples ◽  
Clinical Syndromes ◽  
Old Adult ◽  
Cytopathogenic Effect

Human parechoviruses (HPeV) belonging to the family Picornaviridae are widespread enteric pathogens and are associated with various clinical syndromes in human. At present, 16 HPeV genotypes (HPeV1–16) are known. There is no report on the detection of HPeVs in Central Europe. Aims: The aim of the retrospective study was to detect and characterize HPeVs using molecular methods in cell cultures with “enterovirus-like” cytophatic effect (CPE) archived between 1990 and 2004, in two virology laboratories, in Hungary. Materials and methods: In Laboratory I, fecal samples from children with symptoms of gastroenteritis under the age of 10 years were cultured as a previous routine diagnostic laboratory protocol for “enterovirus”. Cell cultures indicating CPE were archived between 1990 and 2000. In Laboratory II, 2 fecal samples, a liquor and a nasopharyngeal aspirate were re-tested which contained an “enterovirus-like” virus in cell cultures and were positive by HPeV1 neutralization immunosera between 2000 and 2004. Specimens were tested retrospectively for HPeV by reverse transcription–PCR (RT-PCR) method using 5’UTR conserved primers. Specific primers were designed to determine the HPeV structural region (VP0-VP3-VP1). Results: 9 of the 66 archived samples (9.1%) from Laboratory I and all the 4 samples from Laboratory II were found to be HPeV-positive. 10 samples were identified as HPeV1, 2 were HPeV4 and 1 could not be determined. 3 HPeV1 clusters were identified in Laboratory I according to the isolation date originated from years 1990/1991, 1992/1995 and 1998. HPeV1 was detected in clinical syndromes: gastroenteritis (in a 24-years-old adult), recurrent stomatitis aphtosa (in a 42-years-old adult), encephalitis and ataxia cerebellaris acuta in infants and children in Laboratory II. Conclusions: This is the first detection of HPeVs in Central Europe. Detection and genetic characterization of HPeV in available historical samples infected with previously unidentifiable agents with “enterovirus-like” cytopathogenic effect may help to understand the clinical importance and spectrum of the infections and the genetic diversity and evolution of these viruses. Orv. Hetil., 2011, 152, 1007–1012.

scholarly journals The Respiration and Aerobic Glycolysis of Mouse Embryo Cell Cultures Infected with the SE Polyoma Virus.

1963 ◽  
Vol 17 supl. ◽  
pp. 55-58
Author(s):  
B. Skarzynski ◽  
M. Guminska ◽  
Z. Porwit-Bóbr ◽  
J. N. Baptist
Keyword(s):  
Cell Cultures ◽  
Mouse Embryo ◽  
Aerobic Glycolysis ◽  
Embryo Cell ◽  
Polyoma Virus ◽  
Mouse Embryo Cell

The Pathogenesis of Poliomyelitis

PEDIATRICS ◽  
1956 ◽  
Vol 17 (2) ◽  
pp. 309-310
Author(s):  
HERBERT A. WENNER
Keyword(s):  
Nervous System ◽  
Infectious Diseases ◽  
Virus Multiplication ◽  
Poliomyelitis Virus ◽  
Body Tissues

This book, written by one who can speak as an authority, is commended to students of medicine, to practitioners, and to investigators in the infectious diseases. The author has spent more than 20 years investigating the movement of poliomyelitis virus in body tissues. This monograph brings together much of that research and interprets it in the light of the author's experiences. The theme of the monograph is that poliomyelitis virus has a basic neuronocytotropism. Dr. Faber considers the primary sites of virus multiplication to be the peripheral ganglia of the nervous system.

CULTIVATION OF POLIOMYELITIS VIRUS IN TISSUE CULTURE

1952 ◽  
Vol 30 (3) ◽  
pp. 231-245 ◽  
Author(s):  
Joan C. Thicke ◽  
Darline Duncan ◽  
William Wood ◽  
A. E. Franklin ◽  
A. J. Rhodes
Keyword(s):  
Tissue Culture ◽  
Cell Morphology ◽  
Synthetic Medium ◽  
Embryonic Brain ◽  
Human Embryonic Kidney ◽  
Virus Growth ◽  
Nutritive Medium ◽  
Poliomyelitis Virus ◽  
Cytopathogenic Effect ◽  
And Control

This paper presents observations on the growth of Lansing poliomyelitis virus in fluid cultures of various human embryonic and adult tissues. The evidence suggests that viral multiplication has occurred in cultures of monkey testis, human embryonic kidney, and mixtures of brain and cord. Satisfactory virus growth has been obtained particularly in cultures containing human embryonic brain and cord. Virus is present in tissue culture fluids in which the original inoculum has been diluted 10−33.3 by subcultivation. Preliminary observations suggest that a synthetic medium (Mixture 199) devised by Morgan, Morton, and Parker is superior to Hanks–Simms solution as a nutritive medium in such cultures. The cytopathogenic effect of the virus, as revealed by pH determinations and cell morphology, has been noted, although a characteristic pH differential between virus infected and control flasks was not commonly observed. Attempts to grow the virus on a larger scale in Kolle flasks are described.

scholarly journals GLYCOLYSIS IN RAT PERITONEAL MAST CELLS

1965 ◽  
Vol 25 (2) ◽  
pp. 123-128 ◽  
Author(s):  
Nirmal Chakravarty
Keyword(s):  
Carbon Dioxide ◽  
Mast Cells ◽  
Oxygen Uptake ◽  
Glucose Utilization ◽  
Aerobic Glycolysis ◽  
Carbon Dioxide Production ◽  
Anaerobic Glycolysis ◽  
Glycolytic Activity ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells

Glycolytic activity of rat peritoneal mast cells has been measured by the Cartesian ampulla diver technique. The rates of anaerobic glycolysis, expressed as CO2 expelled from a bicarbonate medium, are 1.70 x 10-6 µl and 1.43 x 10-6 µl per cell per hour with and without glucose, respectively. The aerobic glycolysis rate in the presence of glucose, assuming the respiratory quotient to be 1, is 0.93 x 10-6 µl CO2 per cell per hour. It is pointed out that the anaerobic and non-respiratory aerobic carbon dioxide production by mast cells is much higher than the respiratory oxygen uptake reported previously. These values have been interpreted in terms of glucose utilization.

Das Verhalten der alkalischen und sauren Phosphatase in Zellkulturen aus Affennieren nach Infektion mit ECHO-Virus Serotyp 9 und Poliovirus hominis Typ 1

1958 ◽  
Vol 13 (2) ◽  
pp. 71-74 ◽  
Author(s):  
W. Albrecht ◽  
H. K. Mittelstrass ◽  
R. Sauthoff
Keyword(s):  
Poliomyelitis Virus ◽  
Echo Virus

Nach Infektion in vitro gezüchteter Rh-Affennierenzellen mit ECHO-Virus Serotyp 9 und Poliomyelitis-Virus Typ 1 sinkt die Aktivität der sauren Phosphatase in der Zelle stark ab, um nach 9 und 12 Stdn. wieder einen langsamen Anstieg zu zeigen. Die Aktivität der alkalischen Phosphatase bleibt nach Infektion mit ECHO-Virus zunächst unverändert, um nach 9 und 12 Stdn. signifikant anzusteigen. Gleichzeitig ist nach anfänglichem Abfall eine Zunahme der RNS in Beziehung zur DNS zu beobachten. Nach Infektion mit Poliomyelitis-Virus zeigt die Aktivität der alkalischen Phosphatase ein ähnliches, jedoch nicht so ausgeprägtes Verhalten wie nach ECHO-Virus Infektion.Diese Verschiebungen der Phosphatase-Aktivität in Abhängigkeit von der Zeit sind mit der Virusmultiplikation in Zusammenhang zu bringen.

Comparative Biochemical Studies on normal and on Poliomyelitis Virus Infected Tissue Cultures

1958 ◽  
Vol 13 (1) ◽  
pp. 34-41 ◽  
Author(s):  
Ernest Kovacs
Keyword(s):  
Enzyme Inhibitors ◽  
Tissue Cultures ◽  
Irreversible Loss ◽  
Virus Inoculation ◽  
Similar Nature ◽  
Poliomyelitis Virus ◽  
Biochemical Studies ◽  
Temporary Rise ◽  
Cytopathogenic Effect

Es wurden zwei verschiedene Thymo-nucleodepolymerasen viskosimetrisch in Gewebekultur nachgewiesen. Bei beiden Fermenten beobachteten wir eine Aktivitätsabnahme nach Infizierung der Gewebekultur mit Poliomyelitis-Virus. In gleicher Weise hemmt virushaltige Flüssigkeit aus Gewebekultur die Aktivität kristalliner DNA-se von Kalbspankreas und die Aktivität der DNA-sen im Affennieren-Homogenat. Die Fermenthemmung im Organbrei war am größten. Die Versuche zeigen, daß die Hemmwirkung in den 3 verschiedenen Systemen (infizierte Gewebekultur, kristallisierte DNA-se, ungereinigter Organextrakt) ähnlicher Natur sind. Sie scheint von spezifischen und allgemeinen Inhibitoren verursacht zu sein. Während des durch die Viren bedingten Zellzerfalles beobachteten wir eine geringe temporäre Zunahme der DNA-ase-Aktivität; dann folgte die irreversible Abnahme. Die theoretische Bedeutung der Befunde wurde besprochen.Two distinct thymonucleo-depolymerases were demonstrated in tissue cultures (TC), by viscosimetric techniques. An inhibition of their activity was found after virus inoculation and multiplication, in vitro. A similar depressive effect of virus-infected culture fluids was detected upon addition to crystalline DNA-ase of beef-pancreas or to crude enzymes of Rhesus kidney homogenate. The inhibition was more marked in the latter. These assays suggest that the analogous processes observed in the three different testsystems (infected cultivated cells, crystalline DNA-ase, unpurified tissueextract), are of similar nature. The decrease of DNA-ase activity seems to be caused by the presence of specific and general enzyme-inhibitors. During disintegration of the cells due to the cytopathogenic effect of the virus, a small, temporary rise of DNA-ase activities may be found, followed by irreversible loss of these nucleases. Theoretical bearings of the findings were discussed.

scholarly journals Effects of tetrodotoxin and anaesthetics on brain metabolism and transport during anoxia

1972 ◽  
Vol 126 (4) ◽  
pp. 851-867 ◽  
Author(s):  
R. Shankar ◽  
J. H. Quastel
Keyword(s):  
Action Potentials ◽  
Brain Cortex ◽  
Aerobic Glycolysis ◽  
Rat Kidney ◽  
Brain Slices ◽  
Anaerobic Glycolysis ◽  
High Concentration ◽  
Adult Rats ◽  
Brain Cortex Slices ◽  
Cortex Slices

1. Tetrodotoxin, at concentrations at which it abolishes generation of action potentials in the nervous system, enhances by about 300% the rate of anaerobic glycolysis of brain-cortex slices from adult rats, or from adult and infant guinea pigs. This occurs to a greater extent in Ca2+-deficient incubation media than in Ca2+-rich media. Tetrodotoxin has no accelerative effect on cerebral aerobic glycolysis. 2. Tetrodotoxin does not affect the rate of anaerobic glycolysis of 2-day-old rat brain-cortex slices, nor that of adult rat kidney medulla, nor that of an extract of an acetone-dried powder of brain. 3. Tetrodotoxin does not affect the rate of penetration of glucose into brain slices. 4. Its effect is not apparent if it is added 10min or later after the onset of anoxia. 5. Its effect diminishes as the concentration of K+ in the incubation medium is increased while that of Na+ is decreased. 6. Its salient effect, at the onset of anoxia, is to diminish influx of Na+ into, and efflux of K+ from, the brain slices. 7. Substances that promote cerebral influx of Na+, e.g. protoveratrine, sodium l-glutamate, diminish the accelerative action of tetrodotoxin. 8. It is concluded that tetrodotoxin exerts its effect on anaerobic glycolysis by suppressing, at the onset of anoxia, the generation of action potentials and thereby the accompanying influx of Na+ and efflux of K+. It is suggested that glycolytic stimulation occurs because a rate-limiting step, e.g. operation of pyruvate kinase, is stimulated by K+ and depressed by Na+. 9. Local anaesthetics behave in a manner similar to that of tetrodotoxin in enhancing cerebral anaerobic glycolysis. 10. Sodium Amytal has a marked effect at relatively high concentration. 11. Tetrodotoxin diminishes efflux of amino acids, particularly glutamate and aspartate, at the onset of anoxia.

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