Preventive activity of ascorbic acid on lead acetate induced cerebellar damaged in adult Wistar rats

2012 ◽  
Vol 13 (4) ◽  
pp. 99-104 ◽  
Author(s):  
Sunday Abraham Musa ◽  
Iliyasu, Musa Omoniye ◽  
Wilson Oliver Hamman ◽  
Augustine Oseloka Ibegbu ◽  
Uduak Emmanuel Umana
Keyword(s):  
Ascorbic Acid ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Preventive Activity

Ascorbic acid improves extrapyramidal syndromes and corpus striatal degeneration induced by dopamine-2 receptor inhibition in Wistar rats

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Sirajo U. Mujittapha ◽  
Murtala Kauthar ◽  
Ishola O. Azeez ◽  
John C. Oyem
Keyword(s):  
Ascorbic Acid ◽  
Normal Saline ◽  
Wistar Rats ◽  
Corpus Striatum ◽  
Extrapyramidal Symptoms ◽  
Motor Deficit ◽  
Cylinder Test ◽  
Extrapyramidal Syndromes ◽  
Dopamine 2 Receptor ◽  
Inhibition Group

AbstractObjectivesThe prolonged uses of fourth-generation antipsychotics have been implicated in inducing extrapyramidal syndromes characterized by the motor deficit. This was attributed to the loss of dopamine-2 receptor (D2R) signaling. However, ascorbic acid (SVCT2R stimulation) in the brain is proposed to modulate D2R activity. We, therefore, investigated the beneficial roles of ascorbic acid in improving the extrapyramidal symptoms seen in D2R loss.MethodsTwenty adult male Wistar rats of average weight 200 g were distributed randomly into four groups. The control (NS) received normal saline for 28 days, Untreated D2R inhibition group (−D2R) received normal saline for seven days and then subsequently received chlorpromazine for 21 days, D2R inhibition group treated with ascorbic acid (−D2R+SVCT2R) received chlorpromazine for 21 days and was subsequently treated with ascorbate for seven days while the withdrawal group (WG) received chlorpromazine for 21 days and subsequently received normal saline for seven days. Motor deficits were assessed using a rotarod and cylinder test. The corpus striatum was harvested, processed, and stained using H&E and Nissl stains. Cellular density was analyzed using Image J software 1.8.0.ResultsMotor deficit was observed in −D2R animals administered chlorpromazine with less improvement in WG compared to control (p<0.05) in both rotarod and cylinder test. Ascorbic acid (SVCT2R stimulation) significantly (p<0.001) improved the latency of fall and climbing attempts observed in −D2R animals. The density of basophilic trigoid bodies was significantly (p<0.001) restored in −D2R+SVCT2R group, suggesting recovery of neural activity in the corpus striatum. Moreover, the hallmarks of neuronal degeneration were less expressed in the ascorbic acid treatment groups.ConclusionsAscorbic acid putatively ameliorates extrapyramidal symptoms observed in D2R blockage by chlorpromazine in Wistar rats.

Cocos nucifera L. oil alleviates lead acetate-induced reproductive toxicity in sexually-matured male Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Olugbemi T. Olaniyan ◽  
Olakunle A. Ojewale ◽  
Ayobami Dare ◽  
Olufemi Adebayo ◽  
Joseph E. Enyojo ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Histological Analysis ◽  
Cocos Nucifera ◽  
Reproductive Toxicity ◽  
Positive Control ◽  
Negative Control ◽  
Male Wistar Rats ◽  
Cocos Nucifera L

Abstract Objectives Lead primarily affects male reproductive functions via hormonal imbalance and morphological damage to the testicular tissue with significant alteration in sperm profile and oxidative markers. Though, different studies have reported that Cocos nucifera L. oil has a wide range of biological effects, this study aimed at investigating the effect of Cocos nucifera L. oil on lead acetate-induced reproductive toxicity in male Wistar rats. Methods Twenty (20) sexually matured male Wistar rats (55–65 days) were randomly distributed into four groups (n=5). Group I (negative control)—distilled water orally for 56 days, Group II (positive control)—5 mg/kg bwt lead acetate intraperitoneally (i.p.) for 14 days, Group III—6.7 mL/kg bwt Cocos nucifera L. oil orally for 56 days and Group IV—lead acetate intraperitoneally (i.p.) for 14 days and Cocos nucifera L. oil for orally for 56 days. Rats were sacrificed by diethyl ether, after which the serum, testis and epididymis were collected and used for semen analysis, biochemical and histological analysis. Results The lead acetate significantly increases (p<0.05) testicular and epididymal malondialdehyde (MDA) levels, while a significant reduction (p<0.05) in sperm parameters, organ weight, testosterone and luteinizing hormone was observed when compared with the negative control. The coadministration of Cocos nucifera oil with lead acetate significantly increases (p<0.05) testosterone, luteinizing hormone, sperm parameters and organ weight, with a significant decrease (p<0.05) in MDA levels compared with positive control. Histological analysis showed that lead acetate distorts testicular cytoarchitecture and germ cell integrity while this was normalized in the cotreated group. Conclusions Cocos nucifera oil attenuates the deleterious effects of lead acetate in male Wistar rats, which could be attributed to its polyphenol content and antioxidant properties.

Determination of the L-ascorbic acid requirements in Wistar osteogenic disorder Shionogi rats for prolonged carcinogenesis experiments

1996 ◽  
Vol 30 (4) ◽  
pp. 337-346 ◽  
Author(s):  
S. W. Y. Chan ◽  
P. C. Reade
Keyword(s):  
Animal Model ◽  
Ascorbic Acid ◽  
Drinking Water ◽  
Normal Level ◽  
Physical Condition ◽  
Wistar Rats ◽  
Clinical Signs ◽  
Supplement Group ◽  
Palatal Mucosa

Wistar Shionogi rats of the ( od/od) substrain with the osteogenic disorder are unable to synthesize L-ascorbic acid ( L-AA) and appear to be an appropriate animal model for studying the effect of L-AA in carcinogenesis. To determine the minimal L-AA requirements of these animals for prolonged survival in a satisfactory physical condition during experimentation, four concentrations of L-AA (0.33 g/l, 0.67 g/l, 1.67 g/l and 3.33 g/l) were administered via drinking water to four groups of animals ( n=2). Their water intake per cage was recorded three times weekly and the plasma L-AA levels were determined at the start, after 2, 4, 8 and 12 weeks and at the termination of the experiment. To simulate the procedures to be undertaken in oral mucosal carcinogenesis experiments, the animals were gently restrained and a designated amount of sterile NaCl was applied to the palatal mucosa three times a week for 26 weeks. The L-AA supplement group with the lowest concentration (0.33 g/l L-AA) achieved mean plasma levels of 7 ± 1.38 μM, approximately one-eighth that of the normal level (mean plasma L-AA level in outbred Wistar rats was found to be 58 ± 3 μM) whilst those in the higher supplement group (3.33 g/l L-AA) achieved a mean of 18 ± 1.25 μM. All of the animals employed in the present study survived for 26 weeks and showed no clinical signs of L-AA deficiency during this period.

scholarly journals Histomorphological effect of ascorbic acid on mercury chloride-induced changes on the cerebellum of adult wistar rats

2014 ◽  
Vol 31 (04) ◽  
pp. 219-224 ◽  
Author(s):  
A. Ibegbu ◽  
A. Animoku Abdulrazaq ◽  
Ayuba Micheal ◽  
Brosu Daniel ◽  
A. Adamu Sadeeq ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Cerebellar Cortex ◽  
Wistar Rats ◽  
Oral Route ◽  
Mercury Chloride ◽  
Average Weight ◽  
The Central Nervous System ◽  
Living Organisms ◽  
Induced Changes ◽  
Acid Administration

Abstract Introduction. Mercury is one of the most hazardous environmental contaminants to living organisms and the central nervous system has been shown to be the main target. Objective. The present work was aimed at evaluating the effect of ascorbic acid on mercury chloride-induced changes on the cerebellar cortex of adult Wistar rats. Material and method. Thirty Wistar rats of average weight of 200g and were randomly divided into 6 groups of 5 rats each. The animals in Group 1 (control) were administered with distilled water, Groups 2 and 3 were administered with 52mg/kg and 26.25mg/kg body weight of HgCl respectively while Groups 4 and 5 were administered with 52mg/kg of HgCl and 5mg/kg of ascorbic acid and 26.25gm/kg of HgCl and 5mg/kg of ascorbic acid respectively, while Group 6 was administered with 5mg/kg of ascorbic acid. The administration was through oral route, daily for 3 weeks. Results. The result of the biochemical parameters showed a significant increase (P < 0.05) on the mean SOD and LPO values after the administration of mercury chloride and Ascorbic acid. Histological observation of the cerebellar cortex, showed normal histo-morphology in Groups 1 and 6 while, the cerebellum in Groups 2, 3, 4 and 5 showed some degenerative, necrotic and cellular changes. Conclusion. However, ascorbic acid administration has shown to ameliorate the induced degenerative changes in the cerebellum caused by mercury chloride toxicity in Wistar rats.

scholarly journals Protective Effect of Rasayana Herbs on Lead Acetate-induced Testicular Toxicity in Wistar Rats

2021 ◽  
Vol 35 (1) ◽  
pp. 9-15
Author(s):  
Vikas Sharma ◽  
Nagendra S Chauhan ◽  
Umesh K Patil ◽  
Vinod Kumar Dixit
Keyword(s):  
Protective Effect ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Testicular Toxicity

Evaluation of oxidative stress and its modulation by L-Arginine and L-Ascorbic acid in repetitive restraint stress model in Wistar rats

2020 ◽  
Vol 17 ◽  
pp. 100172
Author(s):  
Giridhari Pal ◽  
Tapan Behl ◽  
Vishwajeet Rohil ◽  
Mimansa Khandelwal ◽  
Garima Gupta ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Restraint Stress ◽  
Wistar Rats ◽  
Stress Model

Quantitative Determination of L-Ascorbic Acid by Gas-Liquid Chromatography

1974 ◽  
Vol 57 (6) ◽  
pp. 1346-1348
Author(s):  
Janis E Schlack
Keyword(s):  
Ascorbic Acid ◽  
Lead Acetate ◽  
Chromatographic Method ◽  
Ethanol Solution ◽  
Lead Salt ◽  
Gas Liquid Chromatography ◽  
Trimethylsilyl Derivative ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract A gas-liquid chromatographic method for L-ascorbic acid in foods and food products has been devised. Ascorbic acid is extracted into an ethanol solution, precipitated with lead acetate, regenerated from the lead salt as its trimethylsilyl derivative, and chromatographed on an SE- 30 column. Recoveries averaged 100.1%; the standard deviation was ±0.23%.

Sign in / Sign up

Export Citation Format

Share Document