Evaluation of oxidative stress and its modulation by L-Arginine and L-Ascorbic acid in repetitive restraint stress model in Wistar rats

2020 ◽  
Vol 17 ◽  
pp. 100172
Author(s):  
Giridhari Pal ◽  
Tapan Behl ◽  
Vishwajeet Rohil ◽  
Mimansa Khandelwal ◽  
Garima Gupta ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Restraint Stress ◽  
Wistar Rats ◽  
Stress Model

scholarly journals Static Magnetic Fields Modulate the Response of Different Oxidative Stress Markers in a Restraint Stress Model Animal

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
E. Coballase-Urrutia ◽  
L. Navarro ◽  
J. L. Ortiz ◽  
L. Verdugo-Díaz ◽  
J. M. Gallardo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Magnetic Fields ◽  
Oxidative Damage ◽  
Restraint Stress ◽  
Complementary Therapy ◽  
Stress Model ◽  
Static Magnetic Fields ◽  
Stress Markers ◽  
Model Animal ◽  
New Perspective

Stress is a state of vulnerable homeostasis that alters the physiological and behavioral responses. Stress induces oxidative damage in several organs including the brain, liver, kidney, stomach, and heart. Preliminary findings suggested that the magnetic stimulation could accelerate the healing processes and has been an effective complementary therapy in different pathologies. However, the mechanism of action of static magnetic fields (SMFs) is not well understood. In this study, we demonstrated the effects of static magnetic fields (0.8 mT) in a restraint stressed animal model, focusing on changes in different markers of oxidative damage. A significant increase in the plasma levels of nitric oxide (NO), malondialdehyde (MDA), and advanced oxidation protein products (AOPP), and a decrease in superoxide dismutase (SOD), glutathione (GSH), and glycation end products (AGEs) were observed in restraint stress model. Exposure to SMFs over 5 days (30, 60, and 240 min/day) caused a decrease in the NO, MDA, AGEs, and AOPP levels; in contrast, the SOD and GSH levels increased. The response to SMFs was time-dependent. Thus, we proposed that exposure to weak-intensity SMFs could offer a complementary therapy by attenuating oxidative stress. Our results provided a new perspective in health studies, particularly in the context of oxidative stress.

scholarly journals Optimized models of xenobiotic-induced oxidative stress in HepG2 cells

2021 ◽  
Vol 18 (5) ◽  
pp. 1001-1007
Author(s):  
Yollada Sriset ◽  
Waranya Chatuphonprasert ◽  
Kanokwan Jarukamjorn
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Hepg2 Cells ◽  
Sodium Selenite ◽  
Cellular Injury ◽  
Stress Model ◽  
Total Glutathione ◽  
Tert Butyl Hydroperoxide ◽  
Stress Models

Purpose: To evaluate the molecular impact of ethanol, sodium selenite, and tert-butyl hydroperoxide (TBHP) on oxidant-antioxidant balance in HepG2 cells to establish an optimized oxidative stress model of HepG2 cells. Methods: HepG2 cells were treated with ethanol (10 - 500 mM) and sodium selenite (1 - 10 µM) for 24 and 48 h and with TBHP (50 - 200 µM) for 3 and 24 h, respectively. Biomarkers for cellular injury, ie, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and malondialdehyde (MDA), and for antioxidant system, i.e., superoxide dismutase (SOD), catalase (CAT), and total glutathione content, were determined. Results: All treatments increased the levels of LDH, AST, ALT, and MDA but decreased SOD and CAT activities and the total glutathione content in HepG2 cells. Oxidative stress was induced by these oxidative stressors in HepG2 cells via oxidant-antioxidant imbalance, with TBHP (100 µM, 3 h) acting as a powerful oxidant based on the minimal time to induce oxidative stress. The antioxidants, ascorbic acid and gallic acid, improved oxidant-antioxidant imbalance against xenobiotic-induced oxidative stress in HepG2 cells. Conclusion: These oxidative stress models are suitable for investigating the antioxidant and/or hepatoprotective potential of chemicals, including natural compounds.

Protective Role of L-ascorbic Acid in Oxidative Stress Induced by Repeated Oral Administration of Bifenthrin in Wistar Rats

2016 ◽  
Vol 6 (1) ◽  
pp. 39 ◽  
Author(s):  
Muneer Ahmad Dar ◽  
Rajinder Raina ◽  
Arshad Hussain Mir ◽  
Pawan Kumar Verma ◽  
Nrip Kishore Pankaj ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Oral Administration ◽  
Wistar Rats ◽  
Protective Role

scholarly journals Antioxidative Effects of Ascorbic Acid and Astaxanthin on ARPE-19 Cells in an Oxidative Stress Model

Antioxidants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 833 ◽  
Author(s):  
Sanghyeon Oh ◽  
Young Joo Kim ◽  
Eun Kyoung Lee ◽  
Sung Wook Park ◽  
Hyeong Gon Yu
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Cell Viability ◽  
Lethal Dose ◽  
Ultraviolet B ◽  
Stress Model ◽  
Antioxidative Effect ◽  
Pathogenic Factors ◽  
Intracellular Ros ◽  
Antioxidative Effects

Oxidative stress has been implicated as critical pathogenic factors contributing to the etiology of diabetic retinopathy and other retinal diseases. This study investigated antioxidative effect of ascorbic acid and astaxanthin on ARPE-19 cells within an oxidative stress model induced by common biological sources of reactive oxygen species (ROS). Hydrogen peroxide (H2O2) at concentrations of 0.1–0.8 mM and 20–100 mJ/cm2 of ultraviolet B (UVB) were treated to ARPE-19 cells. Cell viability and intracellular ROS level changes were measured. With the sublethal and lethal dose of each inducers, 0–750 μM of ascorbic acid and 0–40 μM of astaxanthin were treated to examine antioxidative effect on the model. Ascorbic acid at concentrations of 500 and 750 μM increased the cell viability not only in the UVB model but also in the H2O2 model, but 20 and 40 μM of astaxanthin only did so in the UVB model. The combination of ascorbic acid and astaxanthin showed better antioxidative effect compared to each drug alone, suggesting a synergistic effect.

scholarly journals EVALUATION OF ANTIDEPRESSANT ACTIVITY OF ETHANOLIC EXTRACT OF LAGERSTROEMIA SPECIOSA LEAVES IN ALBINO WISTAR RATS

2019 ◽  
pp. 68-74
Author(s):  
VANITA KANASE ◽  
SUNITA VISHWAKARMA
Keyword(s):  
Oxidative Stress ◽  
Normal Saline ◽  
Restraint Stress ◽  
Wistar Rats ◽  
Ethanolic Extract ◽  
Antidepressant Activity ◽  
Control Group ◽  
Dependent Manner ◽  
Lagerstroemia Speciosa ◽  
Dose Dependent

Objective: The objective of the study was to evaluate the antidepressant activity of ethanolic extract of dried leaves of Lagerstroemia speciosa L. (EELS) on acute restraint stress (ARS)-induced depression-like behavior and biochemical alterations in albino Wistar rats. Methods: Thirty rats were randomly divided into five experimental groups. Group-I (normal control) rats received normal saline (2.0 ml/kg, p.o.) daily for 14 days; Group-II (stress control) rats received normal saline (2.0 ml/kg, p.o.) daily for 14 days and subjected to restraint stress on the 13th day. Group-III (standard drug-treated) rats received imipramine (15 mg/kg, p.o.) daily for 14 days and subjected to restraint stress on the 13th day. Groups-IV and V rats were treated with EELS (100 mg/kg and 300 mg/kg, p.o.) daily for 14 days subjected to ARS on the 13th day. Stress-like behavior was assessed by subjecting the rats to behavioral paradigms such as tail-suspension test (TST) and open field test (OFT), 40 min post-restraint stress procedure. Pretest of 10 min for forced swim test (FST) was also given to each rat simultaneously. Then, 23.5 h later, the relevant samples were administered and the main test performed 30 min later. Oxidative stress parameters such as superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and extent of lipid peroxidation (LPO) were analyzed in restraint stress-induced animals and control group, following FST on the 15th day. Statistical Analysis: Expression of data was done as a mean standard error of the mean. The normally distributed data were subjected to one-way analysis of variance followed by Dunnett’s test. *p<0.05 was considered statistically significant. Results: It was observed that L. speciosa L. showed a significant dose-dependent decrease in duration of immobility time in TST and FST when compared with the control group in a dose-dependent manner. The results of OFT also showed a dose-dependent increase in locomotor activity. In addition to behavioral tests, EELS also normalized oxidative stress markers such as CAT, SOD, MDA, and LPO in a dose-dependent manner. Conclusion: The results suggest that the ethanolic extract of L. speciosa L. leaves possesses significant antidepressant property, may be recommended as a supplement for the antidepressant activity.

scholarly journals Oral Ascorbic Acid And α-Tocopherol Protect On Di-(2-Ethyl Hexyl) Phthalate (DEHP) Induced Effects On Gonadotoxicity In The Adult Male Wistar Rats

2020 ◽  
Vol 2 (3) ◽  
Author(s):  
Sunday Adakole Ogli ◽  
Samuel O. Odeh
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Male Infertility ◽  
Sperm Motility ◽  
Wistar Rats ◽  
Sperm Morphology ◽  
Serum Testosterone ◽  
Sperm Cells ◽  
Testosterone Concentration ◽  
Male Wistar Rats

Environmental pollutants such as di-(2-ethylhexyl) phthalate (DEHP) adversely affect reproductive system tissue differentiation and functions with exposure at intrauterine, neonatal or adult stages of life, thereby potentiating male infertility later in life. World health organization estimates a global infertility prevalent rate of 10-15%, and 20-30% among Nigerians, with male factor constituting about 40-50% of infertility cases. This study was designed to investigate the effect(s) of oral vitamins C and E on DEHP induced changes in some semen parameters and serum testosterone concentration in adult Wistar rats. Seventy (70) adult male Wistar rats weighing between 156-250 g were randomised into 7 experimental groups 1, 2, 3, 4, 5, 6 and 7 (group n=10). Animals in groups 1, 2 and 3 were treated with 0.02 mg, 20 mg, 200 mg oral DEHP/kg bw daily respectively, while those in groups 4, 5 and 6, in addition to the above DEHP treatments, were treated with 100 mg ascorbic acid and 67.5 mg α-tocopherol per kg bw daily respectively. Rats in group 7 served as Control and were treated with vehicle. All treatments lasted for 60 days. After, over night fasting, samples of semen and serum were obtained for analysis. Results obtained were expressed as mean ± standard deviation and analyzed for significant differences in means using one way ANOVA and Post Hoc test. Relative to the control reference values, groups exposed to oral DEHP had significant (p<0.05) reduction in sperm count, total sperm motility, active sperm motility, normal sperm morphology, serum testosterone concentration  and serum super oxide dismutase levels to 31.70±18.68x106 cells/mL, 38.60±24.78%, 8.50±5.66%, 38.00±18.00%, 9.56±1.34 ng/mL and 0.017±0.0013 units respectively. Sluggish sperm motility and abnormal sperm morphology significantly (p˂0.01) increased to 39.70±13.05% and 68.50±18.42% respectively. In the groups that had DEHP co-treatments with oral ascorbic acid and α-tocopherol, all studied parameters tended to comparative indifference statistically, with the Controls values. This indicates a protective function against DEHP effects on the studied parameters. The study has shown therefore, that DEHP inflicts oxidative stress in the reproductive system which potentially suppresses serum testosterone concentration with attendant derangements in the qualitative and quantitative sperm cells in adult Wistar rats, and thereby enhancing male infertility. However, the antioxidants ascorbic acid and α-tocopherol protects the gonadal and sperm cells from the harmful effects of DEHP by ameliorating oxidative stress and improving male fertility. This implies that there is need to avoid prolonged exposure to DEHP while encouraging the daily intake of oral ascorbic acid and α-tocopherol. 

scholarly journals Comparative effects of ethanol extract of Talinum triangulare and ascorbic acid on Di-2-ethylhexyl phthalate induced undescended testis in Wistar rats

2019 ◽  
Vol 8 (2) ◽  
pp. 1531-1539
Author(s):  
Olakayode Olaolu Ogundoyin ◽  
Anne Fiyinfoluwa Sanwo
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Wistar Rats ◽  
Undescended Testis ◽  
Seminiferous Tubules ◽  
Testicular Weight ◽  
The Mean ◽  
Group 2 ◽  
Ethylhexyl Phthalate ◽  
Talinum Triangulare

This study compared the effects of Talinum triangulare extract and Ascorbic acid on the oxidative stress caused by Di-2-ethylhexyl-phthalate (DEHP) induced undescended testis (UDT) in Wistar rats. Twenty-four pregnant rats were randomly divided into six groups of four animals each. Group 1(Control) received 2.5ml/kg corn oil for 21 days, Group 2 received 750mg/kg DEHP on gestational days (GD) 14-18, Group 3 received 200mg/kg ascorbic acid for 21 days and 750mg/kg DEHP from GD 14-18 and Group 4 received 200mg/kg Talinum triangulare extract for 21 days and 750mg/kg of DEHP from GD 14-18. The dams were delivered, the resulting pups weighed and sacrificed on postnatal day (PND) 28. Gross examination of the pups, biochemical and histological changes were recorded. Data were analyzed using One-way ANOVA. Group 2 pups had UDT and low birth weight compared to other groups. The mean testicular weight and anogenital distance (AGD) were low in Group 2 pups compared to pups in Groups 3 and 4. The mean values of all the antioxidant enzymes and serum testosterone were low in Group 2 pups compared to other groups. Histological examination of the testes of Group 2 pups revealed moderate vascular congestion within the tunica albuginea and severe atrophy of seminiferous tubules with degenerated Sertoli cells compared to normal findings in other Groups. Although the ameliorative effects of Talinum triangulare is not as potent as that of Ascorbic acid, preconceptional and continuous intake of this vegetable by pregnant women would help to prevent UDT and other associated problems.Keywords: Oxidative stress, Talinum triangulare , testosterone, Undescended testis, Ascorbic acid

scholarly journals Kolaviron and L-Ascorbic Acid Attenuate Chlorambucil-Induced Testicular Oxidative Stress in Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Ebenezer Tunde Olayinka ◽  
Ayokanmi Ore
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Vitamin C ◽  
Wistar Rats ◽  
Lymphocytic Leukaemia ◽  
Protective Capacity ◽  
Butanoic Acid ◽  
Garcinia Kola ◽  
Male Wistar Rats

Chlorambucil (4-[4-[bis(2-chloroethyl)amino]phenyl]butanoic acid) is an alkylating agent, indicated in chronic lymphocytic leukaemia. Kolaviron (KV), a biflavonoid complex fromGarcinia kola,and L-ascorbic acid (AA) are known to protect against oxidative damagein vivo. This study evaluates the protective capacity of KV and AA on chlorambucil-induced oxidative stress in the testes of rat. Twenty male Wistar rats (180–200 g) were randomized into four groups: I: control, II: chlorambucil (0.2 mg/kg b.w.), III: 0.2 mg/kg chlorambucil and 100 mg/kg KV, and IV: 0.2 mg/kg chlorambucil and 100 mg/kg AA. After 14 days of treatments, results indicated that chlorambucil caused significant reduction (P<0.05) in testicular vitamin C and glutathione by 32% and 39%, respectively, relative to control. Similarly, activities of testicular GST, SOD, and CAT reduced significantly by 48%, 47%, and 49%, respectively, in chlorambucil-treated rats relative to control. Testicular MDA and activities of ALP, LDH, and ACP were increased significantly by 53%, 51%, 64%, and 70%, respectively, in the chlorambucil-treated rat. However, cotreatment with KV and AA offered protection and restored the levels of vitamin C, GSH, and MDA as well as SOD, CAT, GST, ACP, ALP, and LDH activities. Overall, kolaviron and L-ascorbic acid protected against chlorambucil-induced damage in the testes of the rat.

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