Stimulatory effects of sodium hypochlorite and ultrasonic treatments on tetrazolium staining and seed germination in vitro of Paphiopedilum SCBG Red Jewel

2016 ◽  
Vol 44 (1) ◽  
pp. 77-90 ◽  
Author(s):  
N. Jiang ◽  
J.X. Zhang ◽  
J.A. Teixeira Da Silva ◽  
J. Duan ◽  
H.T. Liu ◽  
...  
Keyword(s):  
Seed Germination ◽  
Sodium Hypochlorite ◽  
Tetrazolium Staining

scholarly journals The Effect of Sodium Hypochlorite (NaOCl) and Heat Treatments on Seed Germination of Rice: An Approach to Restore Seed Viability

2021 ◽  
pp. 49-53
Author(s):  
Monoj Sutradhar ◽  
Subhasis Samanta ◽  
Brijesh Kumar Singh ◽  
Md. Nasim Ali ◽  
Nirmal Mandal
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Sodium Hypochlorite ◽  
Seed Viability ◽  
Germination Percentage ◽  
Heat Treated ◽  
Mother Plants ◽  
Breaking Seed Dormancy ◽  
One Year

Dormancy in rice serves as a mechanism of survival by protecting the seed from germinating in the mother plants; however, it becomes a problem in germination during sowing in soil or under in vitro conditions. This study was conducted to determine the effect of heat treatment and sodium hypochlorite (NaOCl) treatment of seeds on dormancy alleviation. The seeds included both freshly harvested seeds and one-year-old stored seeds, which were tested for germination after different types of seed treatments. Both the treatments increased the germination percentage in seeds, however, it was lesser in the case of old seeds. The best results were obtained from 2% NaOCl treatment for 24 hrs in new seeds, i.e. 92.84±0.103 % germination percentage (GP). However, the higher GP in old seeds were obtained from 48 hrs of heat-treated seeds i.e. 82.9±0.509 % GP. The results of the experiment revealed that rice seeds start to lose viability within a year due to seed dormancy, but this can be reversed with proper measures. These methods of breaking seed dormancy can be considered effective to break seed dormancy and improve seed germination in rice.

scholarly journals Machine Learning-Mediated Development and Optimization of Disinfection Protocol and Scarification Method for Improved In Vitro Germination of Cannabis Seeds

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2397
Author(s):  
Marco Pepe ◽  
Mohsen Hesami ◽  
Andrew Maxwell Phineas Jones
Keyword(s):  
Neural Network ◽  
Hydrogen Peroxide ◽  
Seed Germination ◽  
Sodium Hypochlorite ◽  
Generalized Regression Neural Network ◽  
Validation Experiment ◽  
Disinfection Procedure ◽  
Artificial Intelligence Models ◽  
Artificial Neural Network Ann

In vitro seed germination is a useful tool for developing a variety of biotechnologies, but cannabis has presented some challenges in uniformity and germination time, presumably due to the disinfection procedure. Disinfection and subsequent growth are influenced by many factors, such as media pH, temperature, as well as the types and levels of contaminants and disinfectants, which contribute independently and dynamically to system complexity and nonlinearity. Hence, artificial intelligence models are well suited to model and optimize this dynamic system. The current study was aimed to evaluate the effect of different types and concentrations of disinfectants (sodium hypochlorite, hydrogen peroxide) and immersion times on contamination frequency using the generalized regression neural network (GRNN), a powerful artificial neural network (ANN). The GRNN model had high prediction performance (R2 > 0.91) in both training and testing. Moreover, a genetic algorithm (GA) was subjected to the GRNN to find the optimal type and level of disinfectants and immersion time to determine the best methods for contamination reduction. According to the optimization process, 4.6% sodium hypochlorite along with 0.008% hydrogen peroxide for 16.81 min would result in the best outcomes. The results of a validation experiment demonstrated that this protocol resulted in 0% contamination as predicted, but germination rates were low and sporadic. However, using this sterilization protocol in combination with the scarification of in vitro cannabis seed (seed tip removal) resulted in 0% contamination and 100% seed germination within one week.

The effect of sodium hypochlorite solutions on in vitro seedling growth and regeneration capacity of sainfoin (Onobrychis viciifolia Scop.) hypocotyl explants

2014 ◽  
Vol 94 (7) ◽  
pp. 1161-1164 ◽  
Author(s):  
Mustafa Yildiz ◽  
Hayrettin Ekiz
Keyword(s):  
Seed Germination ◽  
Sodium Hypochlorite ◽  
Seedling Growth ◽  
Regeneration Capacity ◽  
Hypocotyl Explants ◽  
Onobrychis Viciifolia

Yildiz, M. and Ekiz, H. 2014. The effect of sodium hypochlorite solutions on in vitro seedling growth and regeneration capacity of sainfoin (Onobrychis viciifolia Scop.) hypocotyl explants. Can. J. Plant Sci. 94: 1161–1164. The aim of the present study was to determine the effects of different concentrations (1, 2, 3, 4 and 5%) and temperatures (10, 20 and 30°C) of sodium hypochlorite (NaOCl) solutions on in vitro seed germination, seedling growth and regeneration capacity of sainfoin (Onobrychis viciifolia Scop.) hypocotyl explants. All parameters were negatively affected by concentrations of NaOCl over 2% and temperatures over 20°C while the best results were obtained when seeds were surface-sterilized with 2% NaOCl at 20°C for 30 min. following a pretreatment with 95% ethanol.

scholarly journals DIRECT ORGANOGENESIS OF STEVIA REBAUDIANA IN VITRO USING NODAL EXPLANTS

AGROFOR ◽  
2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Kasundi Mekhala GUNASENA ◽  
Sandun SENARATH
Keyword(s):  
Seed Germination ◽  
Sodium Hypochlorite ◽  
Basal Medium ◽  
Stevia Rebaudiana ◽  
Multiple Shoot ◽  
Seedling Height ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Shoot Bud

Stevia rebaudiana Bertoni is a medicinal herb belonging to the family ofAsteraceae. It is a natural sweetener plant, which is estimated to be 300 timessweeter than cane sugar. In this study, reliable protocol was developed for directorganogenesis of S. rebaudiana using in vitro derived nodal explants. Seeds werecollected from mother plants and they were surface sterilized. To optimize thesurface sterilization procedure, dark color (fertile seeds) seeds were surfacesterilized using different concentrations and in different exposure time ofcarbendazim and sodium hypochlorite (Clorox). Out of different combinations0.2% carbendazim for 5 minutes, 10% sodium hypochlorite for 10 minutes and70% ethanol each followed by two successive washings in sterile distilled waterwas found to be the best for surface sterilization. Two sets of seeds (fresh, stored)were cultured on MS basal medium supplemented with different concentrations ofGA3 for seed germination. According to the results seed viability was lost withtime and it affected seed germination. Seed germination was not affected by GA3,but seedling height was affected by it. Seeds germinated on MS mediumsupplemented with 3.0 mg/L GA3 showed the highest seedling height after 10days. MS basal medium supplemented with different concentrations of BAP andKin were tested for shoot bud and multiple shoot induction. Out of different mediaMs basal medium supplemented with 2.0 mg/L BAP was found to be the bestmedium for shoot bud and multiple shoot induction within 60 days.

scholarly journals Establishment of seed in vitro conditions of Swietenia macrophylla to obtain aseptic seedlings

2019 ◽  
pp. 28-31
Author(s):  
Esmeralda J., Cruz-Gutiérrez ◽  
Luis A. Gómez-Reyes ◽  
Jesús Cortes-Aguilar
Keyword(s):  
Seed Germination ◽  
Laminar Flow ◽  
Sodium Hypochlorite ◽  
Economic Importance ◽  
Swietenia Macrophylla ◽  
Important Place ◽  
Running Water ◽  
Laminar Flow Hood ◽  
Seed Disinfection

The mahogany, Swietenia macrophylla, due to its economic importance, occupies an important place within the regional flora of southern and southeastern Mexico. The objective of this work is to obtain a mahogany seed disinfection protocol for its establishment in vitro. The seeds were left in imbibition for 24 hours, then the seed was washed with running water and soap for 3 minutes. Subsequently, within the laminar flow hood, different treatments were carried out, the control 30 min in captan, treatment 1: 30 min in capture 1.5 min in 70% ethanol and 3 min in 30% chlorine. Treatment 2: 30 min in captan 1.5 in 70% ethanol and 4 min in 30% chlorine. Treatment 3: 30 min in captan, 1.5 min in 70% ethanol and 9 min in 30% sodium hypochlorite. The data were subjected to analysis of variance and comparison test of Tukey means (Tukey, α = 0.05). The variables that were evaluated were germination and contamination in the environment. It was observed that there were no significant differences in seed germination. Regarding contamination, treatment three presented the least contamination.

An In Vitro Analysis of Sodium Hypochlorite Decontamination for the Reuse of Implant Healing Abutments

2020 ◽  
Author(s):  
Ahmad Almehmadi
Keyword(s):  
Sodium Hypochlorite ◽  
Bacterial Culture ◽  
Brain Heart Infusion ◽  
In Vitro Study ◽  
Vitro Study ◽  
Sem Images ◽  
Streptococcus Sanguis ◽  
Implant Dentistry ◽  
Vitro Analysis

Abstract The re-use of healing abutments (HAs) has become common practice in implant dentistry for economic concerns and the aim of this in-vitro study was to assess the effect of sodium hypochlorite (NaOCl) in decontamination of HAs. 122 HAs (Used and sterilized n=107; New n=15) were procured from 3 centers, of which 3 samples were discarded due to perforation in sterilization pouch.  For sterility assessment, the used HAs (n=80) were cultured in Brain Heart Infusion Broth (BHI) and Potato Dextrose Agar (PDA), bacterial isolates were identified in 7 samples. Also, 24 used HAs were stained with Phloxine B, photographed and compared to new HAs (n=5). Scanning electron microscope (SEM) assessed the differences between the two sets of HAs, following which the 7 contaminated HAs along with 24 used HAs from staining experiment (Total=31) were subsequently treated with sodium hypochlorite (NaOCl) and SEM images were observed. About 8.75% of HAs tested positive in bacterial culture; Streptococcus sanguis, Dermabacter hominis, Staphylococcus haemolyticus, and Aspergillus species were isolated. Phloxine B staining was positive for used and sterilized HAs when compared to controls. The SEM images revealed deposits in the used HAs and although treatment with NaOCl eliminated the contamination of cultured HAs, the SEM showed visible debris in the HA thread region. This in-vitro study concluded that SEM images showed debris in used HAs at screw-hole and thread regions even though they tested negative in bacterial culture. The treatment with NaOCl of used HAs showed no bacterial contamination but the debris was observed in SEM images. Future studies on the chemical composition, biological implications, and clinical influence is warranted before considering the reuse of HAs.

Comparative Study to Evaluate the Surface Detail Reproduction of Dental Stone after Immersion in Various Different Disinfectant Solutions, Under Stereomicroscope 10 X Magnification –An In-Vitro Study

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Rathika Rai ◽  
M. A. Easwaran ◽  
K. T. Dhivya
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Control Group ◽  
Immersion Method ◽  
Significant Difference ◽  
Surface Irregularities ◽  
Disinfectant Solution ◽  
Surface Detail ◽  
Group B

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde

Seed-borne fungi of cowpea [Vigna unguiculata (L.) Walp] and their possible control in vitro using locally available fungicides in Botswana.

2016 ◽  
Vol 5 (11) ◽  
pp. 5016 ◽  
Author(s):  
K. B. Khare* ◽  
Loeto D. ◽  
Wale K. ◽  
Salani M.
Keyword(s):  
Seed Germination ◽  
Continuous Light ◽  
Sodium Hypochlorite Solution ◽  
Distilled Water ◽  
Percentage Germination ◽  
Department Of Agriculture ◽  
Hypochlorite Solution ◽  
Cowpea Cultivars ◽  
Petri Plate

Seeds of three cowpea cultivars namely Black eye, ER 7 and Tswana obtained from the Department of Agriculture Research, Gaborone were tested for the presence of seed-borne fungi, and their possible control in vitro using locally available fungicides. Four hundred fifty seeds of each cultivar of cowpea were disinfected with 2% sodium hypochlorite solution for 10 min and washed three times with sterile distilled water before placing them in PDA plates (5 seeds/9 cm Petri plate), incubated at 22±2o C for 12 hour each under continuous light and dark. A total of eight fungi were detected from seeds of cowpea. These were Aspergillus flavus, A. niger, Cylindrocarpon sp., Fusarium equisiti, F. oxyaporum, Penicillium chyrosogenum, Rhizopus oligosporus and R. stolonifer. Rhizopus spp. were dominant fungi recovered from seeds, followed by Penicillium, Aspergillus, Fusarium and Cylindrocarpon. The fungi detected resulted in decay and rotting of seeds, and thereby reducing percentage germination of seeds (22%, 37% and 63 % seed germination in Black eye, ER7 and Tswana varieties of cowpea respectively). Out of four fungicides tested, benlate, captan, dithane M 45 and chlorothalanil. Dithane M45 effectively controlled seed-borne fungi, and enhanced seed germination to an average of 86% (93% germination with no fungi detected in Tswana variety) as compared to chlorothalonile (79%), benlate and captan (77%) and un-treated seeds (45%). The fungal incidence was reduced to 2.3%, 4.3%, 5.3% and 5.3% when seeds were treated with dithane M-45, chlorothalonil, benlate and captan respectively as compared to 62% in non-treated seeds.

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