scholarly journals Antibacterial activity of an aqueous extracts of Alkanna tinctoria roots against drug resistant aerobic pathogenic bacteria isolated from patients with burns infections

2018 ◽  
Vol 7 (1) ◽  
pp. e0104 ◽  
Author(s):  
Ahmed Abduljabbar Jaloob Aljanaby
2018 ◽  
Vol 28 (3) ◽  
pp. 72
Author(s):  
Nehia Hussein ◽  
Noor Ameer Hanon

This study was done to evaluate the antibacterial activity of hot ethanolic and aqueous extracts of Syzygium aromatic (S.aromatic ) and Q.infectoria infectoria (gall) against pathogenic bacteria that cause urinary tract infection (UTI). Such as Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Qualitative detection of the active compounds of the plant extracts was done with chemical reagents. Also, the Qualitative detection of the active groups was further verified with (FTIR). The sensitivity of the UTI causing bacteria was examined against 12 different antibiotics. The results show that E.coli was the most resistant bacteria, resisting 8 from 12 antibiotics; P.aeruginosa resisted 5 from 12 antibiotics; while S.aureus showed resistance to 4 only. The antibacterial activity of the plant extracts was investigated by using four concentrations (40, 60, 80, 100 mg/ml) for each extract against two types of Gram negative bacteria (E.coli, P.aeruginosa), and one type of Gram-positive bacteria (S. aureus). The plant extracts showed different effects on the growth of all bacterial strains. The Minimum Inhibitory Concentration (MIC) and the Minimum bactericidal concentration (MBC) of the ethanolic extracts and aqueous extracts in the study were determined.The kill- time was determined also for each extract.The antioxidant activity of the plants in the study was investigated. Finally, the toxicity of the plant extracts was examined on human red blood cells.


2018 ◽  
Vol 23 (3) ◽  
pp. 131
Author(s):  
Suzana Kristy Satriani Fofied ◽  
Agus Sabdono ◽  
Diah Permata Wijayanti

Staphylococcus aureus and Escherichia coli are pathogenic bacteria agent of many human diseases. Those bacteria infect in various levels and also been antibiotic resistants. Bacterial resistance has become a serious global problem. The purposes of this study were to isolate and identify the symbiotic bacteria of the Sea Urchin that have an antibacterial activity of the Strain Multi-Drug Resistant (MDR) against Staphylococcus aureus and Escherichia coli. Sea Urchin was taken from Panjang island, Jepara Indonesia at 2-3 m depth. The symbiotic bacteria were isolated from Sea Urchin by using dilution method and spread plate method. Phenotypic characteristics was observed on colony shape, color and texture of growing bacteria. While the streak method was used to purify bacterial symbion. The antibacterial activity test was performed using overlay method. The results showed that 3 out of 37 isolates have antibacterial activity against Staphylococcus aureus and Escherichia coli. The BB.03.35 isolate was selected for molecular identification due to the largest inhibitory zone diameter. The sequence of this bacterium showed 97% homology and closely related to Pseudoalteromonas flavipulchra.


2021 ◽  
Author(s):  
Przemysław Biegański ◽  
Łukasz Szczupak ◽  
Manuel Arruebo ◽  
Konrad Kowalski

Recent developments in the field of organometalated antibacterial drugs and metal-based materials with antibacterial activity are reviewed. They emerge as attractive candidates for combating pathogenic bacteria including drug resistant strains.


2021 ◽  
Vol 12 (4) ◽  
pp. 4432-4447

Brocchia cinerea (Vis.) is an Asteraceae-family plant widely used in traditional medicine in Southeastern Morocco to treat several ailments. The aqueous extracts of flowers, leaves, stems and aerial parts of B. cinerea were subjected to a preliminary phytochemical screening test for various constituents. The polyphenols, flavonoids, and tannins contents were determined. Moreover, the antioxidant activity was evaluated by DPPH scavenging assay and ferric reduction antioxidant power. However, the antibacterial activity was evaluated against six pathogenic bacteria. Phytochemical screening of different parts of B. cinerea revealed alkaloids, flavonoids, saponins, tannins, and terpenoids. The leaves extract showed the highest polyphenols concentration (27.15±0.92 mg GAE/g of Ext) and flavonoids (17.46±0.66 mg QE/g of Ext), while flowers extract was highest in tannin (9.57±1.24 mg TAE/g of Extract). All extracts of B. cinerea showed moderate to high antioxidant power, among which the leaves extract demonstrated the strongest antioxidant activity, with an IC50 value of 0.99 mg/mL and an optical density of 1.15. In the case of antibacterial activity screening, leaves extract showed the highest inhibition zone diameters ranging from 9 to 27 mm against the tested bacteria. The results demonstrate that the aqueous extracts of different parts of B. cinerea, especially the leaves, could be developed as pharmaceutical products.


Author(s):  
Bijayanta Sircar ◽  
Shyamapada Mandal

Background: Medicinal plants possess several active components having antimicrobial activity. This study was undertaken to explore the antibacterial activity of Indian olive, Elaeocarpus floribundus, fruit extracts against potential food-borne bacterial isolates.Methods: The ethanolic extracts of olive seed (OSE) and mesocarp-epicarp (OMeE), and the aqueous extracts of olive seed (AqOSE) and mesocarp-epicarp (AqOMeE) were prepared, and analysed qualitatively for phytochemicals. The antibacterial activity of the extracts against food-borne pathogenic bacteria: Bacillus sp., Bacillus cereus, Enterococcus sp. and Corynebacterium sp., was determined by agar-well diffusion method, and minimum inhibitory concentration (MIC) values by agar dilution method.Results: The concentration dependent activity of the extracts against the bacteria was recorded with zone diameter of inhibition 6 - 28 mm for ethanolic extracts, and 7 - 23 mm for aqueous extracts. The ethanolic extracts were confirmed positive for the presence of cardiac glycosides, anthraquinone glycosides, steroids, terpenoids and quinones, while cardiac glycosides, anthraquinone glycosides, steroids, terpenoids, quinones and phenol were detected in the aqueous extracts. The MICs of OSE and OMeE ranged 9.375-12.5mg/ml, and 1.875 - 3.125 mg/ml, respectively, for the test bacteria.Conclusions: The olive fruit extracts contained various bioactive components, and had excellent antibacterial activity against food-borne bacteria. The plant might be useful in the preparation of non-antibiotic antibacterial agents and in the storage of food as well.


Author(s):  
Shumin Sun ◽  
Weichang Dai ◽  
Hansong Yu ◽  
Yuhua Wang ◽  
Xuelin Wang ◽  
...  

As the problems of bacterial resistance and safety of dairy products have become more prominent, alternative medicine for antibiotics in treating cow mastitis is needed. In the present study, antibacterial activity of aqueous and ethanolic extracts of Portulaca oleracea L. and Taraxacum mongolicum Hand.-Mazz against the main pathogenic bacteria (Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae and Streptococcus dysgalactiae) of cow mastitis was evaluated using disc diffusion method. The results showed that aqueous and ethanolic extracts of the two herbs both could inhibit the four pathogenic bacteria of cow mastitis at different level. All extracts of the two herbs displayed the highest antibacterial activities against Escherichia coli than other bacteria. For Portulaca oleracea L., ethanolic extracts had higher antibacterial activities than aqueous extracts except for against Escherichia coli. However, for Taraxacum mongolicum Hand.-Mazz, ethanolic extracts had lower antibacterial activities than aqueous extracts. These results indicate that extracts of Portulaca oleracea L. and Taraxacum mongolicum Hand.-Mazz have the potential to be used in treating cow mastitis.


2021 ◽  
Vol 9 (1) ◽  
pp. 26
Author(s):  
Endang Sulistyarini Gultom ◽  
Tri Hartanti ◽  
Hasnaul Maritsa ◽  
Eko Prasetya

The resistance of pathogenic bacteria to antibiotics is increasing due to antibiotics with incorrect doses, wrong diagnostics, and the wrong target. Bacteria that have been resistant to several antibiotics are called multi-drug resistant organisms (MDRO) bacteria. Bacterial resistance to some antibiotics requires alternative herbal treatments, one of which is the Chromolaena odorata L. Research must therefore be conducted on the antibacterial activity of the ethanol extract fraction of C. odorata L. leaves for MDRO bacteria, such as Staphylococcus lugdunensis methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa extended-spectrum beta-lactamase (ESBL), and Klebsiella pneumoniae ESBL. This study aims to determine the antibacterial activity of the ethanol extract of Kirinyuh leaves (C. odorata L.) and the antibacterial activity of the fractionation against MDRO bacteria. Test results of kirinyuh leaf ethanol extract for S. lugdunensis MRSA, P. aeruginosa ESBL, and K. pneumoniae ESBL bacteria each resulted in an inhibition zone with an average diameter of 11.6 mm (strong), 11.5 mm (strong), and 11.13 mm (strong), respectively. Testing the antibacterial activity of the ethanol fraction against MDRO bacteria can show antibacterial activity against all tested bacteria, namely Fraction 5. The results of the antibacterial activity of fraction 5 against K. pneumoniae ESBL, P. aeruginosa ESBL, and S. lugdunensis MRSA bacteria with the formation of inhibition zones formed of 10.2 mm (strong), 8.8 mm (moderate), and 7.9 mm (moderate), respectively. The results of thin-layer chromatography showed that the secondary metabolites contained in the fifth fraction were terpenoids, steroids, and flavonoids.


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