scholarly journals Phytochemical Composition, Antioxidant and Antibacterial Activities of Extracts from Different Parts of Brocchia cinerea (Vis.)

2021 ◽  
Vol 12 (4) ◽  
pp. 4432-4447
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Pharmaceutical Products ◽  
Aqueous Extracts ◽  
Phytochemical Screening ◽  
Antioxidant Power ◽  
Different Parts

Brocchia cinerea (Vis.) is an Asteraceae-family plant widely used in traditional medicine in Southeastern Morocco to treat several ailments. The aqueous extracts of flowers, leaves, stems and aerial parts of B. cinerea were subjected to a preliminary phytochemical screening test for various constituents. The polyphenols, flavonoids, and tannins contents were determined. Moreover, the antioxidant activity was evaluated by DPPH scavenging assay and ferric reduction antioxidant power. However, the antibacterial activity was evaluated against six pathogenic bacteria. Phytochemical screening of different parts of B. cinerea revealed alkaloids, flavonoids, saponins, tannins, and terpenoids. The leaves extract showed the highest polyphenols concentration (27.15±0.92 mg GAE/g of Ext) and flavonoids (17.46±0.66 mg QE/g of Ext), while flowers extract was highest in tannin (9.57±1.24 mg TAE/g of Extract). All extracts of B. cinerea showed moderate to high antioxidant power, among which the leaves extract demonstrated the strongest antioxidant activity, with an IC50 value of 0.99 mg/mL and an optical density of 1.15. In the case of antibacterial activity screening, leaves extract showed the highest inhibition zone diameters ranging from 9 to 27 mm against the tested bacteria. The results demonstrate that the aqueous extracts of different parts of B. cinerea, especially the leaves, could be developed as pharmaceutical products.

scholarly journals EVALUATION OF ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF FRESH AND FREEZE-DRIED SELECTED FRUIT JUICES

2017 ◽  
Vol 10 (9) ◽  
pp. 156 ◽  
Author(s):  
Naritsara Toobpeng ◽  
Pannapa Powthong ◽  
Pattra Suntornthiticharoen
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Fruit Juices ◽  
Fresh Fruit ◽  
Total Phenolic ◽  
Zone Of Inhibition ◽  
Schleichera Oleosa ◽  
Freeze Dried

  Objective: The objective of this investigation was to assess the biological activity of fresh fruit juices and freeze dried fruit juices including antioxidant and antibacterial activity.Methods: The fruits used in this study were Schleichera oleosa (Lour.) Oken, Carissa carandas L., and Sandoricum koetjape (Burm.f.) Merr. The fresh juice sample (FJS) was extracted and also prepared for freeze drying sample (FDS) by freeze dryer. The antioxidant capacity of FJS and FDS was assessed using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays and determination for total phenolic contents (TPCs) by Folin–Ciocalteu’s reagent. Antimicrobial tests were carried out by agar diffusion methods and evaluated by measuring the zone of inhibition against 10 isolates of pathogenic bacteria.Results: The highest antioxidant activity of FJS and FDS was found in S. oleosa and related to the scavenging effect on DPPH radical of FJS (100% concentration) and FDS (200 mg/ml) was found in the value of 93.05±0.31% and 82.18±0.33%, respectively. TPC of FJS and FDS from S. oleosa at the same concentration as in the DPPH assay was 1,003.53±1.96 ug GAE/300 g of matter and 827.77±23.15 ug GAE/100 ml of matter, respectively. Both FJS and FDS revealed antibacterial activity. The zone of inhibition of FJS was ranging from 8.3±0.6 to 24.0±1.0 mm and FDS values of 7.3±0.5-21.0±0.9 mm. The most potent sample against bacteria was FJS and FDS from S. oleosa which FJSs against Escherichia coli ESBL and FDS against Pseudomonas aeruginosa MDR2.Conclusion: Fruit juices of S. oleosa, C. carandas, and S. koetjape associated with antioxidant activity in the form of both fresh fruit juices and FDS. The result of this study showed that the value of DPPH and TPCs were slightly decreased in FDS compare with fresh juice. Screening of FJS and FDS of C. carandas revealed broad spectrum antibacterial activity. However, FJS of S. oleosa and S. koetjape showed inhibition of growth of bacteria, but few of FDS from these fruits could inhibit limited bacterial isolates. For antibacterial activity against pathogenic bacteria, the samples of fresh and freeze dried exhibited a different degree of activity.

scholarly journals ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF MANGROVE Brugueira gymnorrhiza STEM EXTRACTS AGAINST PATHOGENIC BACTERIA Vibrio cholerae

2021 ◽  
Vol 3 (2) ◽  
pp. 53-61
Author(s):  
Angga Crystal Loasana Yami ◽  
Irmanida Batubara ◽  
Kholis Abdurachim Audah
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Vibrio Cholerae ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Ic50 Value ◽  
Dpph Method

Background : The treatment of some diseases caused by free radicals and pathogenic bacteria usually by using antioxidants and antibiotics. Due to excessive use of antibiotics and other environmental cues, some bacteria are now resistant to certain antibiotics or even to multiple antibiotics. Some Vibrio cholerae bacterial strains are multiresistant to many antibiotics.Objective : The antioxidant and antibacterial activities of Brugueira gymnorrhiza stem extracts against pathogenic bacteria V. cholerae.Method : The B. gymnorrhiza stem was extracted by gradient maceration method. The DPPH method was used to determine the antioxidant activity and the disc diffusion method was used to determine the antibacterial activities. The column chromatography method was used to fractionate the selective extract with the best activity. The LC-MS/MS method was used to identify the compound obtained from the fraction with the best antioxidant and antibacterial activity.Result : Ethyl acetate extract of B. gymnorrhiza stem had the best antibacterial activity with MIC and MBC values of 62.50 mg/L. Ethyl acetate extract also showed the best value of antioxidant activity as indicated by an IC50 value of 255.03 mg/L. The results of fractions test showed that fraction 3 had the best antibacterial and the best antioxidant activities with both the MIC and MBC values of 7.90 mg/L and IC50 value of 348.91 mg/L, respectively.Conclusion : Ethyl acetate extract of B. gymnorrhiza stem has good potential as antioxidant and antibacterial. The compound which is thought as antioxidant and antibacterial from Ethyl acetate extract is 2-Ethyl-4-methyl-1H-imidazole.

scholarly journals Phenolic Content and Antioxidant, Antibacterial Activities of Ethanolic Extract From Lemon Balm and Oregano Plants

2014 ◽  
Vol 11 (1) ◽  
pp. 103-110
Author(s):  
Baghdad Science Journal
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Strong Relationship ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Resistant Isolate ◽  
Lemon Balm

The antioxidant and antibacterial activities of ethanolic extract and phenolic compounds extract of Lemon balm (Melissa officinalis) and Oregano (Oreganum vulgare) plants were studied; the phenolic content and the relationship between these compounds and the above activities were also investigated. The results showed that the Lemon balm had the highest phenolic content (56.5% mg g) and the phenolic content of Oregano was twice lower than Lemon balm. Lemon balm has the highest antioxidant activity which causes lipid peroxidation inhibition activity of linoleic acid (90.5%), this activity was more than ?-tocopherole antioxidant activity (79.3 %). It was found that the main source of antioxidant activity of these plants was belonging to phenolic compounds and the results proved the strong relationship between antioxidant activity and phenolic content. The ethanolic extract of Lemon balm was exhibited strong antibacterial activity ,the inhibition included all bacterial isolates , with highest inhibition zone against Bacillus cereus ( 26 mm), while Oregano did not exhibit clear antibacterial activity, Aeromonas hydrophila was the most resistant isolate . It was obvious from the results of effect of phenolic compounds on bacteria that no relationship between antibacterial activity and phenolic content and the inhibition may be due to other compounds.

scholarly journals Screening of Methanolic and Aqueous Extracts of Lasimorpha senegalensis for Antibacterial Activity

2019 ◽  
pp. 1-7
Author(s):  
Anumudu Obinna H. ◽  
Akaniro Ifunanya R. ◽  
Ofonegbu Mercy N. ◽  
Ibediala John Kennedy C.
Keyword(s):  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Biologically Active ◽  
Diffusion Method ◽  
Aqueous Extracts ◽  
Human Pathogens ◽  
Tetrazolium Chloride ◽  
Test Organisms ◽  
Stem Extract

Traditional medicine, though an old practice in disease prophylaxis and therapy, is still widely employed globally to treat various human ailments. In this study conducted at the Department of Microbiology, University of Nigeria between October 2018 and January 2019, methanolic and aqueous extracts of an aquatic plant Lasimorpha senegalensis were evaluated for antibacterial activities against human pathogens; Escherichia coli and Staphylococcus aureus. Agar well diffusion method was used to determine the potency of L. senegalensis against the test organisms at different concentrations. Also, minimum inhibitory concentration (MIC) was determined by tetrazolium chloride microtiter dilution assay. Results showed that; inhibition zone diameters ranging from 0-14 mm for both test organisms using the plant extracts was less than that of the control (septrin and chloramphenicol) ranging from 0-26 mm. MIC ranged from 62.5 mg/ml to 500 mg/ml, lowest MIC was obtained with methanolic stem extract. Preliminary phytochemical screening revealed the presence of flavonoids responsible for the antibacterial activity. Therefore, L. senegalensis should be considered medicinally important as they contain biologically active compounds with curative potentials against infectious diseases.

Synthesis, antioxidant and antibacterial activity of azo dye-stilbene hybrid compounds

2019 ◽  
Vol 48 (1) ◽  
pp. 84-88 ◽  
Author(s):  
Esmail Rezaei-Seresht ◽  
Aboulfazl Salimi ◽  
Behnam Mahdavi
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Azo Dyes ◽  
Radical Scavenging ◽  
Coupling Reaction ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Butylated Hydroxytoluene ◽  
Azo Compounds ◽  
Content Type

Purpose The purpose of this paper is synthesis and evaluation of antioxidant and antibacterial activities of a series of new azo dyes derived from 4-aminostilbene. Design/methodology/approach First, the starting material 4-aminostilbene was prepared via two successive Wittig and reduction reactions from 4-nitrobenzyl bromide. The obtained 4-aminostilbene was then reacted with some phenols under the normal azo coupling reaction conditions to give five new azo products. Antioxidant activity of the azo compounds was determined by radical scavenging assay using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. Also, the antimicrobial activity of the compounds against one gram-positive and eight gram-negative strains was evaluated based on the inhibition zone using disc diffusion assay. Findings The structures of the azo dyes were identified and characterized by fourier-transform Infrared, 1H nuclear magnetic resonance (NMR) and ultraviolet-visible (UV-V) is spectroscopic methods. All the compounds showed higher antioxidant activity than ascorbic acid (Asc) and butylated hydroxytoluene (BHT) as positive controls. Moreover, the compounds showed lower antibacterial activity than the standard antibiotic vancomycin. Research limitations/implications Excellent antioxidant activity, along with antibacterial activity against Streptococcus pneumoniae and Pseudomonas aeruginosa, was observed for the two synthesized azo dyes. Originality/value Five novel azo dyes based on 4-aminostilbene were synthesized. The dyes have a highly p-extended conjugated structure comprising the phenolic and stilbenic segments, and they indicated good antioxidant activity, so that the two dyes (2c and 2d) even showed much more scavenging activity compared to BHT which is used as an antioxidant agent in food industries. These compounds with highest antioxidant activity also inhibited the growth of S. pneumoniae and P. aeruginosa.

scholarly journals Chemical Composition and Antibacterial Activity of Essential Oils ofTagetes minuta(Asteraceae) against Selected Plant Pathogenic Bacteria

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Martin Muthee Gakuubi ◽  
John M. Wagacha ◽  
Saifuddin F. Dossaji ◽  
Wycliffe Wanzala
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Scientific Basis ◽  
Diffusion Method ◽  
Phytopathogenic Bacteria ◽  
Xanthomonas Axonopodis

The objective of this study was to determine the chemical composition and antibacterial activity of essential oils (EOs) ofTagetes minutaagainst three phytopathogenic bacteriaPseudomonas savastanoipv.phaseolicola,Xanthomonas axonopodispv.phaseoli, andXanthomonas axonopodispv.manihotis. The essential oils were extracted using steam distillation method in a modified Clevenger-type apparatus while antibacterial activity of the EOs was evaluated by disc diffusion method. Gas chromatography coupled to mass spectrometry (GC/MS) was used for analysis of the chemical profile of the EOs. Twenty compounds corresponding to 96% of the total essential oils were identified with 70% and 30% of the identified components being monoterpenes and sesquiterpenes, respectively. The essential oils ofT. minutarevealed promising antibacterial activities against the test pathogens withPseudomonas savastanoipv.phaseolicolabeing the most susceptible with mean inhibition zone diameters of 41.83 and 44.83 mm after 24 and 48 hours, respectively.The minimum inhibitory concentrations and minimum bactericidal concentrations of the EOs on the test bacteria were in the ranges of 24–48 mg/mL and 95–190 mg/mL, respectively. These findings provide a scientific basis for the use ofT. minutaessential oils as a botanical pesticide for management of phytopathogenic bacteria.

scholarly journals Antibacterial activity of Actinomycetes symbiont with seaweeds: a prosperous agent of animal antibacterial

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Bertoka Fajar S.P. Negara ◽  
. Riyanti ◽  
Bintang Marhaeni ◽  
Aradea B. Kusuma
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Pathogenic Bacteria ◽  
Pcr Amplification ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
E Coli ◽  
Amplification Method ◽  
The Relationship

Circulation of synthetic antibacterial chemicals which is used continuously can cause resistance to bacteria. Alternative synthetic antibiotics research then comes up as an urgent need. Recently, the discovery of new antibacterial agents is looking for Actinomycetes. Actinomycetes are one of the active marine bacteria which produce secondary metabolites that could be used as antibacterial. The objective of this study was to evaluate the antibacterial activities of Actinomycetes symbiont with Halimeda macroloba, Gelidiella acerosa, and Turbinaria ornata in Pangandaran beach, Indonesia. A total of 41 Actinomycetes were isolated from 3 species of seaweed. Rep PCR amplification method was used to characterize and identify the relationship between samples. Primer BOX A1R (5’-CTACGGCAAGGCGACGCTGACGCTGACG-3’), 27F (5'-AGTTTGATCMTGGCTCAG-3') and 1492R (5'-TACGGYTACCTTGTTACGACTT-3') was used in amplification 16s DNA. Six isolates from the representative cluster dendogram were selected for secondary metabolites extraction using ethyl acetate solvent, meanwhile agar diffusion method was used as Antibacterial activity assay using E. coli, Proteus sp., and Enterobacter sp. as the bacterial test. The results showed that the activity of secondary metabolite extracts of Actinomycetes symbiont with Halimeda macroloba presented the highest inhibition zone on 3 bacterial tests. In the future, it could be a potential antibacterial agent against animal pathogenic bacteria.

scholarly journals Antibacterial Activity of Aqueous Extracts of Malus Domestica Against Some Bacterial Pathogens

2019 ◽  
Vol 3 (2) ◽  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Malus Domestica ◽  
Maximum Yield ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Water Soluble ◽  
Aqueous Extracts ◽  
Peel Extract

The present study was designed to evaluate the antibacterial activity of aqueous extracts of Malus domestica (peel and pomace) against food-borne pathogens (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus feacalis). Extraction conditions (temperature and time) were optimized to obtain maximum yield by using response surface methodology. Antibacterial potentials of the extracts were examined through disc inhibition zone technique and the results were compared with commercial antibiotic. Results Indicated that maximum antibacterial activity was shown by peel extract against Staphylococcus aureus with average zone of inhibition 19.25±0.89 mm while pomace 12.75±0.71 mm. Maximum recovery for water soluble compounds from peel was obtained at 70ºC followed by 90ºC and 50ºC, which was 16.33, 14.67 and 12.67%, respectively. The extraction yield was also increased but after 30 minutes. The antibacterial activities of peel extract were comparable with commercial antibiotic (20.13±0.83 mm). Escherichia coli were appeared to be the most resistant with inhibition induced by the extracts of peel 15.00±1.07 mm and pomace11.50±0.53mm.

scholarly journals Phytochemical screening and antibacterial activity of rosmarinus officinalis l. against Escherichia coli. local isolates

2015 ◽  
Vol 4 (4) ◽  
pp. 413
Author(s):  
Saima Johar ◽  
Shazia Irfan ◽  
Shehnaz Sher Ahmed ◽  
Rukhsana Jabeen
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Reducing Sugar ◽  
Rosmarinus Officinalis ◽  
Aqueous Extracts ◽  
Phytochemical Screening ◽  
E Coli ◽  
Methanolic Extracts ◽  
Rosmarinus Officinalis L

<p>Rosmarinus officinalis is a medicinal plant which belongs to family Lamiaceae. It is an evergreen bush. It was analyzed for its phytochemical constitution and antibacterial activity. For this purpose flowers, leaves and stem of aqueous and methanolic extracts were used. The tests of phytochemical screening showed that flavonoids, terpenoids, reducing sugar and saponins were present in different concentrations, while tannin was absent in all parts of plant. The antibacterial activity was determined against E. coli with different concentrations (i-e 0.5gm, 1gm and 1.5gm) kept for 24 hours and 48 hours duration. The results showed that the highest mean inhibition zone was observed in the methanolic extracts of leaves 1.5gm kept for 48 hours (28.6 ± 4.261mm).In the stem the methanolic extracts showed the highest inhibition zone with the concentration of 1.5gm (13.4 ± 1.624mm) kept for 24 hours. In the flower the highest inhibition zone in methanolic extracts was observed in 1gm (25.4 ± 2.416mm) kept for 48 hours. In the aqueous extracts the highest inhibition zone values were (17.6 ± 4.029mm, 15.8 ± 2.993mm, 14.4 ± 2.33mm) for stem, flower and leaves respectively.</p>

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