scholarly journals The Effects of Terminalia catappa L. Leaves Extract on the Water Quality Properties, Survival and Blood Profile of Ornamental fish (Betta sp) Cultured

2016 ◽  
Vol 8 (2) ◽  
pp. 241 ◽  
Author(s):  
Rudy Agung Nugroho ◽  
Hetty Manurung ◽  
Dewi Saraswati ◽  
Deasy Ladyescha ◽  
Firman Muhammad Nur
Keyword(s):  
Water Quality ◽  
Blood Cells ◽  
White Blood Cells ◽  
Biology Education ◽  
Ornamental Fish ◽  
Blood Profile ◽  
Quality Properties ◽  
Terminalia Catappa ◽  
Protein Serum ◽  
Blood Profiles

<p>This research aimed to determine the phytochemicals content of <em>Terminalia catappa</em> leaves extract (TCL) and its effects on the survival and blood profiles of ornamental fish (<em>Betta</em> sp) Ninety fish were randomly assigned into six triplicates groups and reared in various concentration of TCL: 0 (control), 125, 250, 375, 500, 625 ppm for 30 days. Temperature, Dissolve oxygen (DO), and pH were monitored during the trial. After 30 days, survival, Red Blood Cells (RBC), White Blood Cells (WBC), haemoglobin (Hb), lymphocyte, and total protein serum (TPS) were analyzed. Based on the phytochemicals test, saponin, triterpenoid, quinon, phenolic, tannin, and flavonoid were detected on the TCL. Temperature and DO were not affected by any concentration of TCL. The lowest pH (5.05) was found in fish medium immersed with 625 ppm of TCL. Adding TCL above 375 ppm resulted in significantly higher survival, RBC, and Hb. The highest WBC was found in fish immersed with 625 ppm whereas the lowest lymphocyte was found in fish immersed with 375 of TCL. However, immersing any various concentration of TCL did not affect on the TPS. In summary, immersing TCL above 375 ppm is beneficial to enhance survival, RBC, WBC, and Hb of <em>Betta</em> sp.</p><p><strong>How to Cite</strong></p><p>Nugroho, R. A., Manurung, H., Saraswati, D., Ladyescha, D. &amp; Nur, F. M. (2016). The Effects of Terminalia catappa L. Leaves Extract on the Water Quality Properties, Survival and Blood Profile of Ornamental fish (<em>Betta</em> sp) Cultured. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(2), 240-247.</p>

scholarly journals The Effect of Breadfruit Leaf Flour (Artocarpus altilis) on Number of Blood Cells And Correlation Between Cholesterol Blood and Meat of Tegal Ducks 10 Weeks Age

2018 ◽  
Vol 19 (3) ◽  
pp. 179
Author(s):  
Elly Tugiyanti ◽  
Emmy Susanti
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Regression Coefficient ◽  
White Blood Cells ◽  
Blood Cholesterol ◽  
Blood Profile ◽  
Artocarpus Altilis ◽  
Randomized Design ◽  
Leaf Powder ◽  
Completely Randomized Design

The purpose of this research was to know the effect of breadfruit leaves flour in feed to blood profile and correlation between cholesterol, HDL and LDL of blood with meat cholesterol of maleTegal duck of 10 weeks old. The material used was Day Old Duck of male Tegal duck  as much as 120 heads with experimental research methods and Completely Randomized Design (CRD). There were 6 treatments: feed without breadfruit leaf powder, feed + breadfruit flour 3% / kg feed, feed + breadfruit flour 6% / kg feed, feed + breadfruit flour 9% / kg feed, feed + flour breadfruit 12% / kg feed, and feed + breadfruit flour 15% / kg feed. Each treatment was repeated 5 times and each replication consisted of 4 ducks. The variables observed were the number of red blood cells, white blood cells, HDL, LDL, blood triglycerides, correlation between cholesterol, HDL and LDL blood with meat cholesterol of male Tegal duck of 10 weeks old.  The result of the analysis showed that the supplementation of breadfruit leaves has significant effect (P <0,05) on the number of red blood cells, white blood cells, HDL, and blood triglyceride. There is a positive correlation between blood cholesterol with duck meat cholesterol which given breadfruit leaf powder. This research can be concluded that the supplementation of breadfruit leaf powder (Artocarpus altilis) can increase the number of red blood cells, white blood cells, blood HDL level but lowers blood cholesterol and triglyceride blood of male Tegal duck of 10 weeks old. Cholesterol meat is affected by blood cholesterol with regression coefficient of 0.780941, influenced by LDL (r = 0.407849), but negatively affected by HDL (r = -0.47022).

scholarly journals Profil Darah Rusa Totol (Axis axis) Betina Sehat di Pusat Inovasi Agroteknologi (PIAT), Universitas Gadjah Mada

2020 ◽  
Vol 38 (1) ◽  
pp. 7
Author(s):  
Dhirgo Adji ◽  
Dian Astuti
Keyword(s):  
Blood Cells ◽  
Mean Corpuscular Volume ◽  
Jugular Vein ◽  
White Blood Cells ◽  
Corpuscular Volume ◽  
Experimental Animals ◽  
Axis Axis ◽  
Spotted Deer ◽  
Edta Tube ◽  
Blood Profiles

Center for Agrotechnology Innovation, Gadjah Mada University (PIAT), one of the place for development and breeding ground of spotted deer in Yogyakarta, Indonesia. The study of blood profiles was intended to allow veterinarian to understand the normal profile of the spotted deer. Fifteen healthy spotted deer belonging to PIAT were used as experimental animals. The deer were drawn its blood through the jugular vein without the use of anaesthesia. Blood was then accommodated in an EDTA tube, centrifuged at 2500 RPM and analyzed using a Mindray BC-2800 haematology analyzer machine. Based on the results of the study of the blood it were known that  Haemoglobine (Hb) was: 11.5 ± 1.703 g/dl, Red blood cells (RBC) was: 9.3 ± 3.580 106/ml, Packed cell volume (PCV) was: 30.8 ± 6.035 %, Mean corpuscular Volume (MCV) was: 36.8 ± 11.102 fl, Mean corpuscular haemoglobine (MCH) was: 15.0 ± 7.313 pg, Mean corpuscular haemoglobine concentration (MCHC) was: 40.0 ± 14.657 g/dl, White blood cells (WBC) was: 6.4 ± 3.096 103/ml , Neutrophils was: 43.4 ± 21.646, % Basophils was: 0.2 ± 0.168 %, eosinophils was: 0.4 ± 0.447 %, lymphocytes was: 53.4 ± 21.546 % and monocytes was: 2.6 ± 2.394 %. From all of the datas it indicated that blood profiles of PIAT’s spotted deers are different from similar spotted deer blood profiles in India.

scholarly journals Comparison the effect of aqueous leaves extract of Melia azedarach L. and Anastatica hierochuntic with antibiotic (Enrofloxaccin) on some blood and biochemical traits of broiler

2012 ◽  
Vol 36 (0A) ◽  
pp. 56-61
Author(s):  
زيادطارق محمد ضنكي
Keyword(s):  
Blood Cells ◽  
Tap Water ◽  
White Blood Cells ◽  
Cholesterol Concentration ◽  
Melia Azedarach ◽  
Biochemical Traits ◽  
Blood Tests ◽  
Serum Cholesterol Concentration ◽  
Wbc Count ◽  
Protein Serum

This experiment were conducted to compare the effect of aqueous leaves extract of Melia azedarach and Anastatica hierochuntic with the antibiotic (Enrofloxaccin) for 49 days, 270 chicks one day old (Ross 308) were randomly distributed into 6 treatments with 3 replicates per treatment and 15 chicks per replicate (45 chicks/treatment). The first treatment were the control, antibiotic (enrofloxaccin) were used in the second treatment at rate of 0.5 ml per liter of tap water, in the 3rd and 4th treatment the aqueous leaves extract of Anastatica hierochuntic were used at rate of 10 and 15 mg/L respectively, and in the 5th and 6th treatment the aqueous leaves extract of Melia azedarach were used at rate of 10 and 15 mg / ml resp. At the end of experiment, blood samples were collected for whole blood tests; Red and White Blood Cells count, biochemical serum tests were also involved ; the concentrate of total cholesterol, glucose, and total protein. The Data showed that using aqueous leaves extract of Anastatica hierochuntic at rate of 10 and 15 mg/L leading to significant increase in serum cholesterol concentration , while , protein serum concentration were significantly decreased in control and in 6th treatment where the aqueous extract of Melia azedarach were used at rate of 15 mg /ml , and there is no significant differences between the treatments in RBC , WBC count , and glucose concentration

scholarly journals Does iron-fortified chewing gum influence the biochemical profile of school-going children (6–10 yrs.)?

2021 ◽  
Vol 33 (1) ◽  
pp. 29-38
Author(s):  
Muhammad Azeem ◽  
Mian Kamran Sharif ◽  
Fais-Ul-Hassan Shah ◽  
Maratab Ali ◽  
Muhammad Amer Nazir ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Blood Cells ◽  
Iron Status ◽  
White Blood Cells ◽  
Maximum Increase ◽  
Blood Profile ◽  
Mean Cell Volume ◽  
Chewing Gums ◽  
The Impact ◽  
Mean Cell Hemoglobin

Iron deficiency has become a common nutritional problem of developing countries, especially in children. This study approached to tackle the issue of iron deficiency by inexpensive fortified food such as chewing gums, which is commonly consumed by children. In this study, iron-fortified chewing gums were prepared by adding ferrous sulfate (FeSO4) and sodium iron EDTA (NaFeEDTA) 30 mg/100 g. An efficacy trial was conducted to determine the impact of iron-fortified chewing gums on the blood profile and iron status of school-going children (n = 300). Results showed maximum increase in blood profile and iron status that is, serum ferritin (10.43%), hemoglobin (3.22%), hematocrit (3.42%), red blood cells (3.05%), mean cell volume (1.55%), mean cell hemoglobin (5.43%), total white blood cells count (9.09%), and platelets count (4.40%) as compared with control whereas decrease in mean cell hemoglobin concentration (1.90%) and neutrophils (3.33%) was also observed. The study concluded that FeSO4 and NaFeEDTA (1:1) fortification of chewing gums is an appropriate approach for mitigating iron deficiency among the target population.

Growth performance and blood profile of West African dwarf goats fed urea treatedwild cocoyam (Colocasia esculentum) mea

2020 ◽  
Vol 45 (1) ◽  
pp. 360-366
Author(s):  
O. Y. Adedeji ◽  
S. O. Odukoya ◽  
O. M. Odetola ◽  
O. A. Awodele ◽  
A. A. Saka
Keyword(s):  
Growth Performance ◽  
Blood Cells ◽  
White Blood Cells ◽  
West African ◽  
Daily Weight Gain ◽  
Average Weight ◽  
Edible Plant ◽  
Blood Profile ◽  
West African Dwarf ◽  
Dietary Treatments

Wild cocoyam is a non edible plant found growing around riversides and can serve as source of energy to ruminants. Hence, a twelve weeks study was conducted to investigate the effect of wild cocoyam – urea meal on the growth performance and blood profile of West African dwarf (WAD) goats. Twelve WAD goats with an average weight of 7.25 ± 0.04kg were randomly allotted into four dietary treatments: T1 (raw wild cocoyam), T2 (urea treated wild cocoyam), T3 (urea treated cooked wild cocoyam) and T4 (urea treated fermented wild cocoyam), replicated thrice in a completely randomized design. Results obtained showed that the experimental diets significantly (p < 0.05) influenced the growth performance. Goats fed urea treated cooked wild cocoyam had the best feed conversion ratio (10.35), the highest values of dry matter intake (405.35 g/day) and daily weight gain (39.17 g/day) when compared to other dietary treatments. The dietary treatments did not significantly (p < 0.05) influence the haematological parameters except the red blood cells (9.62 - 11.67×106 mm3),white blood cells (4.07-9.05×106 mm3) and monocytes (1.00-2.50%). No significant (p>0.05) differences among the dietary treatments were observed in all the serum biochemical indices evaluated except for urea which ranged from 15.50 – 28.70mg/dl. It can therefore be concluded that addition of urea with processing, further improved the utilization of the experimental diets thus improving performance of the animals.

Blood Profile of Lymphocyte Lineage Determining Transcription Factor Gata3 May Influence Malaria Disease Outcome of Children in the South of Benin

2019 ◽  
Author(s):  
Capo-chichi CD
Keyword(s):  
Transcription Factor ◽  
Immune System ◽  
Infectious Diseases ◽  
Blood Cells ◽  
White Blood Cells ◽  
African Countries ◽  
Blood Profile ◽  
Two Samples ◽  
Gata3 Expression ◽  
High Level

Background: Child morbidity in African countries including the Republic of Benin, is mostly attributed to parasitic infectious diseases involving malaria. Molecular disorder often underlies the vulnerability of children immune system to infectious diseases. This reduces the ability of immune system to combat appropriately exogen organisms including parasites larva, bacteria or viruses. GATA-3 is a nuclear protein expressed by immune cells and belongs to a family member of the lymphocyte CD4 lineage determining transcription factors inducible by retinoic acid. Thus, GATA3 is one of the main regulators of lymphocyte T-helper 2 (Th2) cell differentiation into mature CD4+ T cells that mediate immunity tocombat exogenmicroorganism involving plasmodium falciparum (pf). The objective of the study is to investigate if children with low or absence of GATA3 expression will be more vulnerable to pf infection and malaria. Methods: This study has received an institutional ethical approval.Preliminary study carried out on cell lysates of peripheral white blood cells isolated from blood samples used for pf counts in Benin Zonal hospital of Calavi/So-Ava diagnosis laboratory involving eighteen children (5-9 years) admitted for fever associated or not to malaria. Immunoblotting (western blot) technic was used to evaluate GATA3 expression in all samples.The presence of pf was determined with microscopic observation of blood thick smears stained with Giemsa. Results: Among the eighteen samples, normal expression of GATA3 was observed in ten, low expression in six while no expression was observed in two samples. Overall, seven samples were positive for pf counts ranging from 350-1500 per mm3 of red blood cells. Infection with pf was not observed in eleven samples. Conclusion: Immune system of children with low GATA3 and high level of pf count will be more vulnerable to infection. Thus, the absence of GATA3 transcription factor could influence the outcome of malaria disease and render children too weak to fight pf infection.The absence of GATA3 may also render them vulnerable to develop allergy or lymphoma in the future.

scholarly journals Pre and post-gestation blood indices of rabbit does fed graded levels of neem (Azadirachta indica) leaf meal

2020 ◽  
Vol 45 (5) ◽  
pp. 15-22
Author(s):  
O. Y. Ayo-Ajasa ◽  
L. T. Egbeyale ◽  
J. A. Abiona ◽  
A. O. Fafiolu
Keyword(s):  
Azadirachta Indica ◽  
Total Protein ◽  
Blood Cells ◽  
Control Diet ◽  
White Blood Cells ◽  
Live Weight ◽  
Blood Profile ◽  
Blood Indices ◽  
Leaf Meal ◽  
Randomized Design

This study was conducted to investigate the effect of neem leaf meal on the blood status of rabbit does before and after gestation. Atotal of twenty-four (24) rabbit does of mixed breeds, with initial average live weight of 1975g were randomly allocated into four (4) experimental dietary treatments; each of which was replicated three (3) times with two (2) rabbits per replicate. The rabbits were fed diets containing 0%, 2.5%, 5.0%, and 7.5% neem (Azadirachta indica) leaf meal. Parameters measured included packed cell volume (PCV), haemoglobin, red blood cells (RBC), white blood cells (WBC) and its differentials for haematology while total protein, globulin, albumin, cholesterol, alkaline phosphate (ALP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured for biochemical indices. All data generated were subjected to one-way Analysis of Variance in a Completely Randomized Design. The findings from this study at pre-gestation showed that neem leaf meal (NLM) significantly (p<0.05) influenced basophil and serum total protein, globulin and glucose. Basophil was significantly reduced in rabbits fed 5.0% NLM (0.33%) while the serum components were affected in rabbits fed 2.5% NLM. The result at post-gestation showed no significant (p>0.05) difference in all the parameters measured exceptneutrophils. Neutrophils were significantly (p<0.05) reduced in rabbits on the control diet (26.67%) while rabbits on NLM diets recorded similar values. From the serum component, cholesterol was significantly (p<0.05) highest in rabbits fed 5.0% NLM (62.33mg/dL) and lowest in those fed 2.5% NLM (49.67mg/dL). It was, therefore, concluded that inclusion of NLM in rabbit does' diet up to 7.5% had no adverse effect on the health status of the animals as the values obtained for neutrophils and cholesterol were still within the normal range of rabbit blood profile.

scholarly journals Toxicity bioassay and sub-lethal effects of diazinon on blood profile and histology of liver, gills and kidney of catfish, Clarias gariepinus

2019 ◽  
Vol 79 (2) ◽  
pp. 326-336 ◽  
Author(s):  
A. M. Al-Otaibi ◽  
H. F. A. Al-Balawi ◽  
Z. Ahmad ◽  
E. M. Suliman
Keyword(s):  
Blood Cells ◽  
Clarias Gariepinus ◽  
White Blood Cells ◽  
Blood Parameters ◽  
Activity Level ◽  
Blood Profile ◽  
Histological Changes ◽  
Blood Indices ◽  
Secondary Lamellae ◽  
Wbc Count

Abstract The catfish, Clarias gariepinus, was exposed to different acute concentrations (5-10 mg/l) of diazinon and the Lc50 value was recorded as high as 7.3 mg/l for 96 hours. The fishes exposed to three sub-lethal levels of diazinon (0.73, 1.095 and 1.46 mg/l) for 30 days showed that the pesticide induces changes in different blood parameters. Number of red blood cells (RBC), haemoglobin level and haematocrit values were elevated whereas white blood cells (WBC) count was reduced. Various blood indices like mean corpuscular volume (MCV), mean corpuscular haemoglobine (MCH) and mean corpuscular haemoglobine concentration (MCHC) vary insignificantly in the fish treated with diazinon. Glucose level and activity level of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) was increased in Clarias gariepinus exposed to diazinon. Protein level in plasma of fish treated with pesticide was changed but the change was insignificant. Histological changes in the liver, gills and kidney of fishes exposed to diazinon were apparent when compared to control. Hepatocytes necrosis and bleeding were more distinct in the fishes exposed to pesticide. Glomerulus hypertrophy and bleeding in kidneys; and fusion and degeneration of secondary lamellae and epithelial hyperplasia in gills were also observed in the exposed fish.

scholarly journals Blood Profile of Growing Rabbits Fed Pro-Vitamin A Fortified Cassava Peel Meal Based Diets

Agricultura ◽  
2021 ◽  
Vol 18 (1-2) ◽  
pp. 1-8
Author(s):  
Peter-Damian Chukwunomso JIWUBA ◽  
Stanley Uzochukwu ILO ◽  
Wisdom AMADURUONYE ◽  
Nonye Lilian AZODO ◽  
Chinenye UZOMA
Keyword(s):  
Vitamin A ◽  
Blood Cells ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Blood Parameters ◽  
Metabolizable Energy ◽  
Blood Profile ◽  
Randomized Design ◽  
Serum Biochemical ◽  
Cassava Peel

The effect of feeding pro-vitamin A cassava peel meal (PCPM) based diets to growing rabbits (n=48) for 61 days on their haematology and serum biochemical parameters was investigated. T1, T2, T3, and T4 diets were formulated with inclusion levels of 0, 15, 30, and 45%, respectively. In a completely randomized design, the rabbits were randomly allocated to four experimental groups of twelve animals each, with four rabbits constituting a replicate. On the last day of the study, blood samples were taken from each animal and analyzed for haematological and serum biochemical indices. The results of the proximate composition of experimental diets revealed that T2, T3, and T4 had high (p<0.05) ash values. T1 diet had higher (p<0.05) metabolizable energy (ME) in comparison with T3, and T4 diets. Red blood cells (RBC) and white blood cells (WBC) were improved (p<0.05) significantly in 15, 30, and 45% PCPM inclusions. T3 and T4 had better (p<0.05) packed cell volume (PCV) values, haemoglobin (Hb), mean cell haemoglobin (MCH), and mean cell haemoglobin concentration (MCHC) than T1 and T2. The treatment groups (T2, T3 and T4) had significantly higher (p<0.05) total protein, globulin, creatinine, total bilirubin, and aspartate amino transferase (AST). Cholesterol and urea levels were lowered significantly (p<0.05) in T3 and T4. All of the blood parameters were within the normal physiological range for clinically healthy rabbits, indicating that PCPM was beneficial to the rabbits' blood formation and health. T4 group had the best results and was recommended for enhanced rabbit production.

scholarly journals The Effects of Ketapang (Terminalia catappa) Bark Crude Extract on Inhibition of Aeromonas hydrophila Growth and Blood Cells of the Infected Carp (Cyprinus carpio)

Rekayasa ◽  
2018 ◽  
Vol 11 (2) ◽  
pp. 87
Author(s):  
Maftuch Maftuch ◽  
Heny Suprastyani ◽  
Ellana Sanoesi ◽  
Gede A. A. K. Putra ◽  
Mirsa F. Putra ◽  
...  
Keyword(s):  
Water Quality ◽  
Cyprinus Carpio ◽  
Crude Extract ◽  
Aeromonas Hydrophila ◽  
Blood Cells ◽  
Infected Fish ◽  
Terminalia Catappa ◽  
Carp Cyprinus Carpio

<em><span lang="IN">The objectives of the research were to investigate the effect of ketapang (Terminalia catappa) bark crude extract (KBCE) on Aeromonas hydrophila growth and blood cells of the A. hydrophila-infected carp (Cyprinus carpio). The Research was conducted by using completely randomized design. There were four (750, 850, 950, and 1,050 ppm) and three treatments (730, 750, and 770 ppm) were used for in vitro and in vivo experiments respectively. Data were analyzed by analysis of variation and LSD. The dose to investigate MIC of KBCE were 750, 850, 950 and 1,050 ppm.  The MIC of KBCE was 750 ppm. On in vivo investigation, number of erythrocytes were 224.67x10, 332.67x10, and 417.00x10 cells/mm for 730, 750 and 770 ppm KBCE, respectively. For leukocytes, the amount were 735.89x10, 684.78x10, and 652.67x10 cell/mm for 730, 750 and 770 ppm KBCE, respectively. In addition, the water quality were as follows 24.0 – 25.9C of temperature, 7.0 – 7.7 of pH, and 3.9 – 4.8 mg/L of DO. In conclusion, we revealed that KBCE able to act as antibacterial against A. hydrophila. Furthermore, it also induced the total erythrocyte in infected fish without giving a notable effect on water quality.</span></em>

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