Hydroxycitric acid ameliorates high-fructose-induced redox imbalance and activation of stress sensitive kinases in male Wistar rats

2016 ◽  
Vol 0 (0) ◽  
Author(s):  
Ramalingam Sripradha ◽  
Magadi Gopalakrishna Sridhar ◽  
Nachimuthu Maithilikarpagaselvi
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Food Intake ◽  
Wistar Rats ◽  
Density Lipoprotein ◽  
Mitogen Activated Protein Kinase ◽  
Stress Index ◽  
Redox Imbalance ◽  
Hydroxycitric Acid ◽  
Male Wistar Rats

AbstractExcess fructose consumption causes dyslipidemia, oxidative stress, and various complications. Hydroxycitric acid (HCA), one of the principal components of the fruitMale Wistar rats (n=40) were randomly divided into four groups with 10 rats in each group. The rats were fed with either standard rodent diet or 60% fructose diet and administered with HCA at a dose of 400 mg/kg body wt/day for 10 weeks. Body weight was measured once a week, and food intake was noted daily. At the end of the study, lipid profile and oxidative stress parameters were estimated. Expressions of stress sensitive kinases were analyzed in liver homogenates.Fructose-fed rats displayed elevated body weight, higher levels of plasma total cholesterol (TC), triacylglycerol (TAG), non-high-density lipoprotein cholesterol (non HDL-C), malondialdehyde (MDA), total oxidant status (TOS), oxidative stress index (OSI), lower levels of HDL-C, glutathione (GSH), glutathione peroxidase (GPx), and total antioxidant status (TAS). Fructose feeding caused higher phosphorylation of stress sensitive kinases ERK ½ and p38. Administration with HCA lowered body weight, food intake, TAG, non-HDL-C, MDA, TOS, and OSI and elevated GSH, GPx, and TAS levels. Reduced phosphorylation of ERK ½ and p38 mitogen-activated protein kinase (MAPK) was observed upon HCA treatment.Thus, HCA improved fructose induced redox imbalance and activation of stress sensitive kinases through its hypolipidemic effects.

Effect of gallic acid on renal biochemical alterations in male Wistar rats induced by ferric nitriloacetic acid

2006 ◽  
Vol 25 (9) ◽  
pp. 523-529 ◽  
Author(s):  
Lakshmi Prasad ◽  
Tajdar Husain Khan ◽  
Tamanna Jahangir ◽  
Sarwat Sultana
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Gallic Acid ◽  
Antioxidant Enzyme ◽  
Wistar Rats ◽  
Nitrilotriacetic Acid ◽  
Decarboxylase Activity ◽  
Causative Role ◽  
Biochemical Alterations ◽  
Male Wistar Rats

The present study is an effort to identify a potent chemopreventive agent against various diseases (including cancer) in which oxidative stress and cell proliferation plays an important causative role. This study was designed to investigate the effect of gallic acid against ferric nitrilotriacetic acid (Fe-NTA)-induced carcinogen/drug metabolizing phase I and phase II enzymes, anti-oxidative parameters, kidney markers, tumour promotion markers and lipid peroxidation (LPO) in kidney of male Wistar rats. Fe-NTA (9 mg Fe/kg body weight, intraperitoneally) caused significant depletion in the detoxification and antioxidant enzyme armoury with concomitant elevation in renal LPO, serum creatinine, blood urea nitrogen, hydrogen peroxide generation, ornithine decarboxylase activity and [3H]thymidine incorporation into renal DNA. However, pretreatment of animals with gallic acid (10 and 20 mg/kg body weight) resulted in a significant decrease in the levels of the parameters measured (P < 0.001). Renal glutathione content (P < 0.001), glutathione metabolizing enzyme (P < 0.001) and antioxidant enzyme levels were also recovered to a significant level (P < 0.001). The enhanced reduced glutathione level and enzyme activities involved in xenobiotic metabolism and maintaining antioxidant status of cells are suggestive of a chemopreventive efficacy of gallic acid against Fe-NTA-mediated oxidative stress, toxicity and cell proliferative response in Wistar rats.

scholarly journals Protective role of melatonin against adipose-hepatic metabolic comorbidities in experimentally induced obese rat model

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0260546
Author(s):  
Mary J. Obayemi ◽  
Christopher O. Akintayo ◽  
Adesola A. Oniyide ◽  
Ayodeji Aturamu ◽  
Olabimpe C. Badejogbin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Food Intake ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Liver Lipid ◽  
Control Group ◽  
Metabolic Dysfunction ◽  
High Fat ◽  
Male Wistar Rats

Background Adipose and hepatic metabolic dysfunctions are critical comorbidities that also aggravate insulin resistance in obese individuals. Melatonin is a low-cost agent and previous studies suggest that its use may promote metabolic health. However, its effects on some comorbidities associated with obesity are unknown. Herein, we investigated the hypothesis that melatonin supplementation would attenuate adipose-hepatic metabolic dysfunction in high fat diet (HFD)-induced obesity in male Wistar rats. Materials and methods Twenty-four adult male Wistar rats (n = 6/group) were used: Control group received vehicle (normal saline), obese group received 40% high fat diet, melatonin-treated group received 4 mg/kg of melatonin, and obese plus melatonin group received 40% HFD and melatonin. The treatment lasted for 12 weeks. Results HFD caused increased food intake, body weight, insulin level, insulin resistance and plasma and liver lipid but decreased adipose lipid. In addition, HFD also increased plasma, adipose and liver malondialdehyde, IL-6, uric acid and decreased Glucose-6-phosphate dehydrogenase, glutathione, nitric oxide and circulating obestatin concentration. However, these deleterious effects except food intake were attenuated when supplemented with melatonin. Conclusion Taken together, the present results indicate that HFD exposure causes adipose-hepatic metabolic disturbance in obese animals, which are accompanied by oxidative stress and inflammation. In addition, the present results suggest that melatonin supplementation attenuates adipose-hepatic metabolic dysfunction, accompanying obesity by suppression of oxidative stress/inflammation-dependent mechanism and increasing circulating obestatin.

Preventive effect of curcumin on inflammation, oxidative stress and insulin resistance in high-fat fed obese rats

2016 ◽  
Vol 13 (2) ◽  
Author(s):  
Nachimuthu Maithilikarpagaselvi ◽  
Magadi Gopalakrishna Sridhar ◽  
Rathinam Palamalai Swaminathan ◽  
Ramalingam Sripradha
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Skeletal Muscle ◽  
Body Weight ◽  
Density Lipoprotein ◽  
High Fat ◽  
Oxidative Stress Parameters ◽  
Tnf Α ◽  
Male Wistar Rats ◽  
Fat Feeding

Abstract: The present study investigated the beneficial effects of curcumin on inflammation, oxidative stress and insulin resistance in high-fat fed male Wistar rats.: Five-month-old male Wistar rats (n=20) were divided into two groups (10 rats in each group). Among the two groups, one group received 30 % high-fat diet (HFD) and another group received 30 % HFD with curcumin (200 mg/kg body weight). Food intake, body weight and biochemical parameters were measured at the beginning and at the end of the study. After 10 weeks, oxidative stress parameters in skeletal muscle and hepatic triacylglycerol (TAG) content were estimated. Histological examinations of the liver samples were performed at the end of the experiment.: High-fat feeding caused increase in body weight, liver and adipose tissue mass. Rats fed with HFD showed increased levels of fasting plasma glucose, insulin, Homeostasis Model Assessment for Insulin resistance (HOMA-IR), total cholesterol (TC), TAG, very low density lipoprotein cholesterol (VLDL-c) and decreased high-density lipoprotein cholesterol (HDL-c). There was also increase in the plasma inflammatory markers [tumor necrosis factor-α (TNF-α), C-reactive protein (CRP)] and skeletal muscle oxidative stress parameters [malondialdehyde (MDA), total oxidant status (TOS)] in these rats. In addition, high-fat feeding increased liver TAG content and caused fat accumulation in the liver. Treatment with curcumin significantly reduced body weight, relative organ weights (liver, adipose tissue), glucose, insulin and HOMA-IR. Curcumin supplementation decreased plasma levels of TC, TAG, VLDL-c, TNF-α and increased HDL-c. Administration of curcumin also reduced MDA, TOS in skeletal muscle, hepatic TAG content and liver fat deposition.: Curcumin supplementation improved HFD-induced dyslipidemia, oxidative stress, inflammation and insulin resistance.

scholarly journals Effects of Consumption of Rooibos (Aspalathus linearis) and a Rooibos-Derived Commercial Supplement on Hepatic Tissue Injury bytert-Butyl Hydroperoxide in Wistar Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
B. D. Canda ◽  
O. O. Oguntibeju ◽  
J. L. Marnewick
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Wistar Rats ◽  
Tissue Injury ◽  
Body Weight Gain ◽  
The Body ◽  
Hepatic Tissue ◽  
Herbal Tea ◽  
Butyl Hydroperoxide ◽  
Male Wistar Rats

This study investigated the antioxidative effect of rooibos herbal tea and a rooibos-derived commercial supplement ontert-butyl hydroperoxide- (t-BHP-) induced oxidative stress in the liver. Forty male Wistar rats consumed fermented rooibos, unfermented rooibos, a rooibos-derived commercial supplement, or water for 10 weeks, while oxidative stress was induced during the last 2 weeks via intraperitoneal injection of 30 µmole oft-BHP per 100 g body weight. None of the beverages impaired the body weight gain of the respective animals. Rats consuming the rooibos-derived commercial supplement had the highest (P<0.05) daily total polyphenol intake (169 mg/day) followed by rats consuming the unfermented rooibos (93.4 mg/day) and fermented rooibos (73.1 mg/day). Intake of both the derived supplement and unfermented rooibos restored thet-BHP-induced reduction and increased (P<0.05) the antioxidant capacity status of the liver, while not impacting on lipid peroxidation. The rooibos herbal tea did not affect the hepatic antioxidant enzymes, except fermented rooibos that caused a decrease (P<0.05) in superoxide dismutase activity. This study confirms rooibos herbal tea as good dietary antioxidant sources and, in conjunction with its many other components, offers a significantly enhanced antioxidant status of the liver in an induced oxidative stress situation.

scholarly journals Lipoic Acid Exacerbates Oxidative Stress and Lipid Accumulation in the Liver of Wistar Rats Fed a Hypercaloric Choline-Deficient Diet

Nutrients ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1999
Author(s):  
Lidia V. Kravchenko ◽  
Ilya V. Aksenov ◽  
Nikolay S. Nikitin ◽  
Galina V. Guseva ◽  
Ludmila I. Avrenyeva ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Liver Disease ◽  
Lipoic Acid ◽  
Lipid Accumulation ◽  
Wistar Rats ◽  
Weight Ratio ◽  
Body Weight Ratio ◽  
Deficient Diet ◽  
Male Wistar Rats

Non-alcoholic fatty liver disease (NAFLD) is currently estimated as the most prevalent chronic liver disease in all age groups. An increasing body of evidence obtained in experimental and clinical data indicates that oxidative stress is the most important pathogenic factor in the development of NAFLD. The study aimed to investigate the impact of α-lipoic acid (LA), widely used as an antioxidant, on the effects of a hypercaloric choline-deficient diet. Male Wistar rats were divided into three groups: control diet (C); hypercaloric choline-deficient diet (HCCD), and hypercaloric choline-deficient diet with α-lipoic acid (HCCD+LA). Supplementation of HCCD with LA for eight weeks led to a decrease in visceral adipose tissue/body weight ratio, the activity of liver glutathione peroxidase and paraoxonase-1, plasma, and liver total antioxidant activity, as well as an increase in liver/body weight ratio, liver total lipid and triglyceride content, and liver transaminase activities compared to the HCCD group without LA. In conclusion, our study shows that α-lipoic acid detains obesity development but exacerbates the severity of diet-induced oxidative stress and lipid accumulation in the liver of male Wistar rats fed a hypercaloric choline-deficient diet.

scholarly journals One-month of high-intensity exercise did not change the food intake and the hypothalamic arcuate nucleus proopiomelanocortin and neuropeptide Y expression levels in male Wistar rats

2019 ◽  
Vol 53 (1) ◽  
pp. 8-13 ◽  
Author(s):  
Nazli Khajehnasiri ◽  
Homayoun Khazali ◽  
Farzam Sheikhzadeh ◽  
Mahnaz Ghowsi
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Mrna Expression ◽  
High Intensity ◽  
Wistar Rats ◽  
Arcuate Nucleus ◽  
The Body ◽  
High Intensity Exercise ◽  
Hypothalamic Arcuate Nucleus ◽  
Male Wistar Rats

AbstractObjective. The hypothalamic arcuate nucleus proopiomelanocortin (POMC) and neuropeptide Y (NPY) circuitries are involved in the inhibition and stimulation of the appetite, respectively. The aim of this study was to investigate the effects of one-month lasting high-intensity exercise on the POMC mRNA and NPY mRNA expression in the above-mentioned brain structure and appetite and food intake levels.Methods. Fourteen male Wistar rats (250±50 g) were used and kept in the well-controlled conditions (22±2 °C, 50±5% humidity, and 12 h dark/light cycle) with food and water ad libitum. The rats were divided into two groups (n=7): 1) control group (C, these rats served as controls) and 2) exercised group (RIE, these rats performed a high-intensity exercise for one month (5 days per week) 40 min daily with speed 35 m/min. The total exercise time was 60 min. The body weight and food intake were recorded continuously during the experiments.Results. The results showed relative mRNA expression of POMC and NPY estimated in the hypothalamic arcuate nucleus. There were no significant differences in the NPY and POMC mRNAs expression levels and food intake between C and RIE groups.Conclusions. The present data indicate that one-month regular intensive exercise did not alter the levels of NPY and POMC mRNAs expression (as two important factors in the regulation of appetite) in the hypothalamic arcuate nucleus and food intake suggesting that this type of exercise itself is not an appropriate procedure for the body weight reduction.

scholarly journals Changes in body composition after thermal injury in the rat

1979 ◽  
Vol 42 (3) ◽  
pp. 367-375 ◽  
Author(s):  
G. A. Al Shamma ◽  
C. C. Goll ◽  
T. B. Baird ◽  
J. Broom ◽  
G. A. Nicholas ◽  
...  
Keyword(s):  
Weight Loss ◽  
Wound Healing ◽  
Body Composition ◽  
Body Weight ◽  
Food Intake ◽  
Body Fat ◽  
Wistar Rats ◽  
Thermal Injury ◽  
Energy Deficit ◽  
Male Wistar Rats

1. The effects on body composition, measured by direct techniques, of a controlled 25% body-surfacearea thermal injury have been studied in two groups of forty male Wistar rats.2. The extent of weight loss in the animals was directly related to their energy deficit resulting from a combination of injury, food intake and rate of wound healing.3. Body fat proved the most labile source of tissue energy, decreasing to a minimum of approximately 30 g/kg body-weight.4. Relationships between water and fat, and water and protein seen in control animals were not significantly different in the traumatized group.

scholarly journals Ameliorative Effect of Pleurotus ostreatus on Lipid Levels and Atherogenic Indices in Hyperlipidemic Rats

2019 ◽  
pp. 1-6
Author(s):  
N. L. Nwobi ◽  
O. S. Usiobeigbe ◽  
R. O. Osaro ◽  
J. C. Nwobi
Keyword(s):  
Body Weight ◽  
Pleurotus Ostreatus ◽  
Wistar Rats ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Lipid Levels ◽  
Reduced Body ◽  
Atherogenic Indices ◽  
Male Wistar Rats

Aim: To evaluate the effects of Pleurotus ostreatus on the lipid profile and atherogenic indices in Hyperlipidemic rats. Study Design, Place and Duration of Study: This case-control study was done for 60 days between March and April, 2017 at the department of Medical Laboratory Science and Department of Chemical Pathology, Babcock University, Ogun State, Nigeria. Methodology: Thirty male wistar rats weighing 117-130 g were divided randomly into 3 groups: Normolipidemic (NL) rats (fed with standard rodent chow), Hyperlipidemic (HL) rats (fed with standard rodent chow + duck yolk and reused oil), Hyperlipidemic Treated (HL+T) rats (fed with standard rodent chow + duck yolk and reused oil + 5% Pleurotus ostreatus powder).  Changes in the animal body weights were measured in this study. Serum was obtained from fasting blood samples for the standard biochemical analyses of total cholesterol (TC), triglycerides (TG), High density lipoprotein cholesterol (HDL-C), creatinine, urea, Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST). Low density lipoprotein (LDL), very low density lipoprotein (VLDL), TC/HDL, LDL/HDL and Log (TG/HDL) ratios were calculated. Results: The HL+T rats compared to HL rats had significantly reduced body weight, TC, TG, LDL, VLDL, TC/HDL, LDL/HDL and Log(TG/HDL) by 19.59%, 14.38%, 15.82%, 25.52%, 15.83%, 28.89%, 20.24% and 27.27% respectively (p ≤ 0.05) but recorded no significant change in HDL-C (p > 0.05). Creatinine, urea, AST and ALT did not show any significant change in HL rats and HL+T rats (p > 0.05). Conclusion: Treatment of hyperlipidemic male wistar rats with Pleurotus ostreatus reduced body weight, lipid levels (TC, TG, LDL, VLDL) and atherogenic indices (TC/HDL, LDL/HDL, Log (TG/HDL)) and appeared to have no detrimental effects on the liver and kidneys. These findings may provide insights and scientific basis for the promotion of the use of Pleurotus ostreatus in controlling hyperlipidemia and associated complications.

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