Textile Scaffolds for Tissue Engineering – Near Future or Just Vision?

In dental practice there is an increasing need for predictable therapeutic protocols able to regenerate tissues that, due to inflammatory or traumatic events, may suffer from loss of their function. One of the topics arising major interest in the research applied to regenerative medicine is represented by tissue engineering and, in particular, by stem cells. The study of stem cells in dentistry over the years has shown an exponential increase in literature. Adult mesenchymal stem cells have recently been isolated and characterized from tooth-related tissues and they might represent, in the near future, a new gold standard in the regeneration of all oral tissues. The aim of our review is to provide an overview on the topic reporting the current knowledge for each class of dental stem cells and to identify their potential clinical applications as therapeutic tool in various branches of dentistry.

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Tissue Engineering in Musculoskeletal Tissue: A Review of the Literature

Surgeries ◽

10.3390/surgeries2010005 ◽

2021 ◽

Vol 2 (1) ◽

pp. 58-82

Author(s):

Mary Bove ◽

Annalisa Carlucci ◽

Giovanni Natale ◽

Chiara Freda ◽

Antonio Noro ◽

...

Keyword(s):

Tissue Engineering ◽

Living Cells ◽

Physical Factors ◽

Clinical Use ◽

Muscle Loss ◽

Review Of The Literature ◽

Musculoskeletal Tissue ◽

Volumetric Muscle Loss ◽

Structural And Functional Properties ◽

Near Future

Tissue engineering refers to the attempt to create functional human tissue from cells in a laboratory. This is a field that uses living cells, biocompatible materials, suitable biochemical and physical factors, and their combinations to create tissue-like structures. To date, no tissue engineered skeletal muscle implants have been developed for clinical use, but they may represent a valid alternative for the treatment of volumetric muscle loss in the near future. Herein, we reviewed the literature and showed different techniques to produce synthetic tissues with the same architectural, structural and functional properties as native tissues.

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Functional Tissue Engineering Through Biofunctional Macromolecules and Surface Design

MRS Bulletin ◽

10.1557/mrs2010.526 ◽

2010 ◽

Vol 35 (8) ◽

pp. 584-590 ◽

Cited By ~ 9

Author(s):

Lorenzo Moroni ◽

Pamela Habibovic ◽

David J. Mooney ◽

Clemens A. van Blitterswijk

Keyword(s):

Tissue Engineering ◽

Tissue Formation ◽

Cellular Phenotype ◽

3D Scaffolds ◽

Surface Design ◽

Engineered Tissue ◽

Porous Biomaterials ◽

Near Future

AbstractTissue engineering is a rapidly developing discipline that has already entered the clinics and will tremendously change patient management in the near future. The aim of classical tissue engineering is to heal damaged or diseased tissues and organs through the combination of cells, biological factors, and porous biomaterials. The resulting, engineered tissue must possess appropriate functional properties to replace or supplement the targeted tissue. This is still a challenge to overcome before tissue-engineered products can be considered a complete success. Classical tissue engineering approaches rely on the use of mature cells expanded in vitro and transplanted alone or seeded in passive 3D scaffolds, which can lead to the loss of cellular phenotype and production of nonfunctional extracellular matrix. An emerging strategy involves the design of bioactive 3D scaffolds with instructive properties able to recruit cells in situ and direct tissue formation. Here, we present and discuss recent efforts to achieve smart scaffolds encompassing macromolecular biofunctionalization and surface design.

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Supramolecular Hydrogels for Protein Delivery in Tissue Engineering

Molecules ◽

10.3390/molecules26040873 ◽

2021 ◽

Vol 26 (4) ◽

pp. 873

Author(s):

Yaqi Lyu ◽

Helena S. Azevedo

Keyword(s):

Tissue Engineering ◽

Protein Delivery ◽

Building Blocks ◽

Water Soluble ◽

High Expectations ◽

Hydrogel Network ◽

Non Covalent Interactions ◽

Near Future ◽

Direct Incorporation ◽

Covalent Interactions

Therapeutic proteins, such as growth factors (GFs), have been used in tissue engineering (TE) approaches for their ability to provide signals to cells and orchestrate the formation of functional tissue. However, to be effective and minimize off-target effects, GFs should be delivered at the target site with temporal control. In addition, protein drugs are typically sensitive water soluble macromolecules with delicate structure. As such, hydrogels, containing large amounts of water, provide a compatible environment for the direct incorporation of proteins within the hydrogel network, while their release rate can be tuned by engineering the network chemistry and density. Being formed by transient crosslinks, afforded by non-covalent interactions, supramolecular hydrogels offer important advantages for protein delivery applications. This review describes various types of supramolecular hydrogels using a repertoire of diverse building blocks, their use for protein delivery and their further application in TE contexts. By reviewing the recent literature on this topic, the merits of supramolecular hydrogels are highlighted as well as their limitations, with high expectations for new advances they will provide for TE in the near future.

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Effects of Hydroxyapatite/Silk Fibroin/Chitosan Ratio on Physical Properties of Scaffold for Tissue Engineering Application

Materials Science Forum ◽

10.4028/www.scientific.net/msf.872.261 ◽

2016 ◽

Vol 872 ◽

pp. 261-265 ◽

Cited By ~ 1

Author(s):

Wassanai Wattanutchariya ◽

Atitaya Oonjai ◽

Kittiya Thunsiri

Keyword(s):

Tissue Engineering ◽

Physical Properties ◽

Pore Structure ◽

Silk Fibroin ◽

Engineering Application ◽

Porous Scaffolds ◽

Tissue Engineering Application ◽

Pass Through ◽

Near Future ◽

Interconnected Pore

This study reports the effects of the mixing ratio of hydroxyapatite (HA), silk fibroin (SF) and chitosan (CS) on the physical properties of the scaffold used in tissue engineering. Experimental design based on mixture design was implemented to investigate the degradation rate of the scaffolds fabricated from various ratios of those biomaterials. Furthermore, pore morphology and pore size were evaluated to confirm the compatibility of the scaffold topography for cell growth and adhesion. The results from the study showed that all ratios, except pure HA solution, can be fabricated into porous scaffolds with an interconnected pore structure and appropriate pore sizes to allow all types of human cells to pass through. Furthermore, the scaffold solutions with high CS ratio resulted in a uniform pore structure and lower rates of biodegradation. Therefore, CS is recommended as the main structure because it provides the highest resistance to biodegradation. The scaffolds from various ratios may be applied for different tissue replacements in the near future.

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Tissue Engineering in Musculoskeletal Tissue: Review of Literature

10.20944/preprints202011.0697.v1 ◽

2020 ◽

Author(s):

Mary Bove ◽

Annalisa Carlucci ◽

Giovanni Natale ◽

Chiara Freda ◽

Antonio Noro ◽

...

Keyword(s):

Tissue Engineering ◽

Living Cells ◽

Physical Factors ◽

Clinical Use ◽

Muscle Loss ◽

Review Of Literature ◽

Musculoskeletal Tissue ◽

Volumetric Muscle Loss ◽

Structural And Functional Properties ◽

Near Future

Tissue engineering, also called “regenerative medicine”, refers to attempt to create functional human tissue from cells in laboratory. This is a field that uses living cells, biocompatible materials, suitable biochemical and physical factors and their combinations, to create tissue-like structures.. To date, no tissue engineered skeletal muscle implants have been developed for clinical use, but it may represent a valid alternative to treat volumetric muscle loss in the near future. Herein, we reviewed the literature and showed different techniques to produce synthetic tissues with the same architectural, structural and functional properties of native tissues.

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Surgery and Operating Room for Restoring Organs: Organ Regeneration by Tissue Engineering in the Near Future

Surgery and Operating Room Innovation ◽

10.1007/978-981-15-8979-9_6 ◽

2020 ◽

pp. 57-62

Author(s):

Mitsuo Miyazawa ◽

Masato Watanabe ◽

Yoshihisa Naito ◽

Yasumitsu Hirano ◽

Keizo Taniguchi ◽

...

Keyword(s):

Tissue Engineering ◽

Operating Room ◽

Organ Regeneration ◽

Near Future

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Innovative Biomaterials in Bone Tissue Engineering

Materials International ◽

10.33263/materials11.002012 ◽

2019 ◽

Vol 1 (1) ◽

pp. 002-012

Author(s):

Denisa- Alexandra Florea ◽

Ecaterina Andronescu ◽

Alexandru Mihai Grumezescu

Keyword(s):

Tissue Engineering ◽

Bone Tissue ◽

Cost Effective ◽

Healthcare Systems ◽

Tissue Level ◽

Bone Trauma ◽

The World ◽

The Usa ◽

Innovative Biomaterials ◽

Near Future

Currently, the transplant crisis is one of the main concerns in the healthcare systems all over the world, the lack of donors and the persons which are on the waiting list for a transplant being higher from year to year. The diseases at the bone tissue level are affecting about 75M in USA, Europe and Japan, the need of treatments in this field becoming clear. During the last decades, the USA spent more than 20 billion dollars on treatments for bone trauma and more than 300.000 spinal fusions were conducted only in 2005. Moreover, the International Osteoporosis Foundation stated that the number of hip fractures may increase by four by 2050. Therefore, considering the data reported for the last decades and the predictions made for the near future, there are two main directions which must be considered: the drawbacks of the current treatments and the economic impact of the available options. In this regards, Tissue Engineering is relatively new field in the regenerative medicine area, which aims to develop cost-effective alternatives for different diseases/trauma in order to restore the function of a tissue and to undertake the transplant crisis.

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Recombinant Proteins-Based Strategies in Bone Tissue Engineering

Biomolecules ◽

10.3390/biom12010003 ◽

2021 ◽

Vol 12 (1) ◽

pp. 3

Author(s):

Marina Paulini ◽

Iván Nadir Camal Ruggieri ◽

Melina Ramallo ◽

Matilde Alonso ◽

José Carlos Rodriguez-Cabello ◽

...

Keyword(s):

Tissue Engineering ◽

Regenerative Medicine ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Recombinant Proteins ◽

Complex Structure ◽

High Impact ◽

Special Importance ◽

Near Future ◽

Very High

The increase in fracture rates and/or problems associated with missing bones due to accidents or various pathologies generates socio-health problems with a very high impact. Tissue engineering aims to offer some kind of strategy to promote the repair of damaged tissue or its restoration as close as possible to the original tissue. Among the alternatives proposed by this specialty, the development of scaffolds obtained from recombinant proteins is of special importance. Furthermore, science and technology have advanced to obtain recombinant chimera’s proteins. This review aims to offer a synthetic description of the latest and most outstanding advances made with these types of scaffolds, particularly emphasizing the main recombinant proteins that can be used to construct scaffolds in their own right, i.e., not only to impregnate them, but also to make scaffolds from their complex structure, with the purpose of being considered in bone regenerative medicine in the near future.

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Recombinant Collagens for Tissue Engineering Scaffolds

10.17488/rmib.30.1.7 ◽

2017 ◽

Author(s):

◽

A. Paredes-Puerto

Keyword(s):

Tissue Engineering ◽

Biomedical Applications ◽

Production Capacity ◽

Clinical Applications ◽

Research Development ◽

Expression Systems ◽

Tissue Engineering Scaffolds ◽

Structure Quality ◽

Broad Application ◽

Near Future

Due to its biocompatibility, handling and industrial production capacity, collagens have been increasingly attractive in the manufacture of scaffolds for Tissue Engineering. The aim of the present work was to present an analysis on the progress in research, development and production of human recombinant collagens, expression systems and their uses in Tissue Engineering. A review of the international scientific peer-reviewed literature in databases such as Scopus, PubMed and Google Scholar was done and that relevant to our objective was employed. The development of human recombinant collagens was found to be significant, and currently the expression systems, like bacteria and plants, show advantages over structure quality and biocompatibility, albeit with still restricted yields. However, there is narrow information about its applications in Tissue Engineering, mostly studied for cartilage and bone, in animal models and clinical studies. We did not include patents in the study, thus our findings are limited to scholar data. The present work presents the most recent advances in the engineering of recombinant collagens and their biomedical applications in the manufacture of tissues with potential clinical applications. The potential of recombinant collagens in regenerative medicine is promising and more research is needed that might allow a broad application in the near future.

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