scholarly journals Association of LEP- and CTSF-genotypes with levels of meat quality PSE, NOR and DFD in pigs of large white breed of Ukrainian selection

2020 ◽  
Vol 7 (1) ◽  
pp. 14-23
Author(s):  
I. Bankovska ◽  
Y. Oliinychenko ◽  
V. Balatsky ◽  
T. Buslyk ◽  
S. Hryshchenko ◽  
...  
Keyword(s):  
Meat Quality ◽  
Genetic Markers ◽  
Population Analysis ◽  
Dna Typing ◽  
Rflp Analysis ◽  
Large White ◽  
Genetic Population ◽  
Large White Breed ◽  
White Breed ◽  
Meat Samples

Aim. To study the distribution of genotypes by polymorphisms of the leptin (LEP SNP g.3469 T > C, LEP SNP g.2845 A > T) and cathepsin F (CTSF SNP g.22 C > G) genes according to the quality levels PSE, NOR and DFD meat in Large White pig breed of Ukrainian selection and to fi nd associations of genetic markers with the total indicator of meat quality. Methods. We studied meat samples (m. longissimus dorsi) from 102 pigs of Ukrainian Large White breed, raised to the weight at slaughter of 120 ± 5 kg in the experimental farm “Stepne” of the Poltava region, Ukraine. The ranking of muscle tissue was carried out according to the total indicator of meat quality (TM). PCR-RFLP analysis was used for DNA typing. The associations between genetic markers and TM were determined using ANOVA. Results. Genetic population analysis of Large White breed pigs of Ukrainian selection by genetic markers LEP SNP g.3469 T > C, LEP SNP g.2845 A > T and СTSF SNP g.22 C > G was carried out. The informative value of LEP SNP g.2845 A > T and СTSF SNP g.22 C > G, according to the calculated polymorphism information content, was optimal for associative studies (PIC = 0.311 and 0.373, respectively). The distribution of meat samples by quality levels PSE, NOR and DFD was performed. Most of them had traits of moderately expressed (n = 22) and weakly expressed (n = 59) PSE defect (light, soft, exudative meat). The calculated coeffi cients of Chuprov’s mutual conjugation between the genotypes for the studied SNPs and meat quality levels showed a moderate relationship between the genotypes for LEP SNP g.2845 A > T and CTSF SNP g.22 C > G and meat quality levels, К = 0.26 and 0.24, respectively. According to the results of ANOVA, the differences were found between homozygous and heterozygous CTSF SNP g.22 C > G genotypes in terms of the total indicator of meat quality. Conclusions. The meat of heterozygous pigs for CTSF SNP g.22 C > G (g.22GC) is characterized by a higher total indicator of meat quality (4.6) compared to the meat of homozygous animals g.22GG (4.2, p ≤ 0.05) and g.22CC (3.9, p ≤ 0.01).

scholarly journals Use of specialized breeds for increase of pork meat quality

1970 ◽  
Vol 21 ◽  
pp. 238-242
Author(s):  
O. V. Boiko ◽  
O. V. Vashchenko ◽  
M. S. Nebylytsia
Keyword(s):  
Meat Quality ◽  
Pork Meat ◽  
Selection Methods ◽  
Large White ◽  
Muscle Area ◽  
Large White Breed ◽  
National University ◽  
White Breed ◽  
Conventional Methods

Aim. Matching ability to study for reproductive, fattening and meat qualities of pigs and to identify the most effective combinations of genotypes national and foreign selection. Methods. Studies conducted in conditions of industrial technology of LLB "Mayak-Agro" Cherkassy region. Groups formed by from counterpart’s methods. Assessment fattening and meat quality was carried out by conventional methods (Polivoda A., 1977). The chemical composition and physical properties of meat were determined by conventional methods in condition of biotechnology laboratories Cherkassy National University. B. Khmelnitsky. Results. The highest quantity offspring obtained by crossing hybrids with boars red white girdle (RWG) and pietrain (P) (11.6–11.8 piglets), with big fetus – when two breed crossed Ukrainian large white breed (ULW) sows of landrace (L) boars and sows of crossbred boars two bread from (P) (1.8 kg, P>0.99). By weight of one head at weaning the best results are obtained by crossing two breeds ewes from boars (RWG) and (P) – 18.1–17.8 kg (P > 0.999). Maximum saving values obtained when combined hybrids (ULW x L) with boars breed Duroc Ukrainian selection “Steppes” (DUSS) – 94.8 %. Conclusions. To improve the meat quality of pork, the best option is a combination of two breeds pig breeds (ULW x L) of boars (P) and (RWG). Keywords: hybridization, a specialized type, industrial crossing, heterosis, muscle area.

Evaluation of haploid diversity of sows of the Large White breed of the pedigree factory of the State enterprise “Experimental enterprise “imeni 9 Sіchnja”

2020 ◽  
pp. 68-73
Author(s):  
Ruslan Hodyna ◽  
Valeriia Matiiuk ◽  
Tetiana Buslyk ◽  
Konstiantyn Pochernyaev
Keyword(s):  
Rflp Analysis ◽  
Ion Exchange Resin ◽  
The State ◽  
State Enterprise ◽  
Production Environment ◽  
Large White ◽  
Mitochondrial Haplotypes ◽  
Large White Breed ◽  
Pcr Rflp ◽  
White Breed

The development and use of effective selection programs accelerated the genetic improvement of breeds, advances in feeding technology allowed for optimal nutrition, and the creation of identical and strictly controlled production conditions, a uniform and strictly controlled production environment led to the replacement of local pig breeds with highly productive transboundary breeds. This development of the industry has led to increased concern about the erosion of genetic resources. The number of purebred boars of the Large White breed in 1973 amounted to 98.3 % of the total number of boars of this breed, and sows - 38.9 %. These data allow us to assume that significant haploid diversity could persist in the individual pedigree factory of the Large White breed. Thus, the subject of our study was the assessment of the haploid diversity of the Large White sows of the pedigree factory of the State enterprise “Experimental enterprise “imeni 9 Sіchnja”, the Institute of Pig Breeding and AIP NAAS of Ukraine. For the study, samples of the bristles of 25 sows of the Large White breed of the SE "Experimental enterprise "imeni 9 Sіchnja" of the Institute of Pig Breeding and AIP NAAS of Ukraine, representing all families. DNA isolation from bristles was performed using a Chelex-100 ion exchange resin. Mitochondrial haplotypes were determined using PCR-RFLP analysis according K. F. Pochernyaev (2016). Among the sample of breeding pigs of the Large White breed of the SE “Experimental enterprise “imeni 9 Sіchnja”, mitochondrial haplotypes B1, B2, C, G, and J1 were identified. In previous studies, seven mitochondrial haplotypes were found among pigs of the Large White breed of Ukraine, which were found with different frequencies. The haplotypes G (14.1 %), J1 (13.3 %) and N (5.91 %) were found with the highest frequency; much less often - haplotypes A (0.3 %), B1 (1.9 %), C (9.2 %) and L (2.2 %). Thus, our studies have shown that the mitochondrial haplotypes B1, B2, C, G, J1 found in the sample of breeding pigs of the Large White breed of the SE «Experimental enterprise «imeni 9 Sіchnja» typical for pigs of the Large White breed of Ukraine except haplotype B2. Important for the restoration of the Mirgorod breed of pigs is the existence in the herd of pigs of the Large White breed of the SE “Experimental entewrprise“imeni 9 Sіchnja” animals having haplotype B1 inherent to Myrgorod breed. Thus, our studies have shown that in sows of the Large White breed breeding farm SE “Experimental enterprise “imeni 9 Sіchnja”, significant haploid diversity remains, which must be maintained at a high level in the future. Key words: pigs, the Large White breed, haplotype, mitochondrial genome, PCR-RFLP.

Polymorphisms in the regulatory region of the porcine MYLPF gene are related to meat quality traits in the Large White breed

Meat Science ◽  
2016 ◽  
Vol 113 ◽  
pp. 104-106
Author(s):  
M.T. Ryan ◽  
A.M. O'Halloran ◽  
R.M. Hamill ◽  
G.C. Davey ◽  
M. Gil ◽  
...  
Keyword(s):  
Meat Quality ◽  
Regulatory Region ◽  
Quality Traits ◽  
Large White ◽  
Meat Quality Traits ◽  
Large White Breed ◽  
White Breed

scholarly journals Продуктивність в’єтнамських вислочеревних свиней та їх помісей

2013 ◽  
pp. 73-75
Author(s):  
В. Є. Усачова
Keyword(s):  
Meat Quality ◽  
Growth And Development ◽  
Multiple Pregnancy ◽  
Large White ◽  
Slaughter Weight ◽  
Reproductive Ability ◽  
Large White Breed ◽  
White Breed

Наведено результати дослідження репродуктивних, відгодівельних і м’ясних якостей свиней в’єтнамської вислочеревної породи «в чистоті» та в поєднанні з плідниками великої білої породи. Встановлено, що за використання схрещування досягається поліпшення відтворювальної здатності свиноматок за багато-, великоплідністю, живою масою одного поросяти і гнізда при відлученні. Підсвинки в’єтнамської вислочеревної породи поступалися помісному молодняку за інтенсивністю росту і розвитку. Відгодівля помісних генотипів сприяла поліпшенню відгодівельних та м’ясних якостей. The results of the study of reproductive, fattening and meat quality of pigs in Vietnam loose belly breed «in a cleanness» and in crossing with the male hogs of large white breed are given in the article. It is set that the use of crossing leads to improvement of reproductive ability of sows on multiple pregnancy, large fetus, to living mass of one pigling. Gilts of the Vietnamese loose belly breed yielded to the crossbreed sapling on intensity of growth and development and slaughter-weight. Fattening of crossbreed genotypes was instrumental in the improvement of fattenings and meat qualities.

scholarly journals Influence of imported pigs breeds to Lithuanian white pigs meat production and quality

2007 ◽  
Vol 23 (5-6-1) ◽  
pp. 67-75 ◽  
Author(s):  
V. Jukna ◽  
C. Jukna ◽  
K. Saikevicius
Keyword(s):  
Meat Quality ◽  
Cross Section ◽  
Quality Parameters ◽  
Meat Production ◽  
Drip Loss ◽  
Pork Meat ◽  
Large White ◽  
Large White Breed ◽  
White Breed ◽  
Favorable Conditions

Pig breeding in Lithuania has still remained a traditional branch of agriculture. Pork meat in meat production balance of the country makes over 40 %. Many authors stated that in case of favorable conditions for crossbreeding combinations it becomes possible to improve successfully pigs? meat properties and meat quality (Michalska et al, 2000; Zaiadilov et al, 2005; Popov, 2005; Jukna et al, 2005; Suslina et al, 2005; Dziaugys et al, 1997). In order to improve pork meat quality in Lithuania it seems important to know which breeds of pigs are most purposeful for breeding with Lithuanian White pigs. The report presents the data about the crossing of Lithuanian White (LW) pigs with the Landraces (L), Yorkshires (Y) and boars of Large White breed. The most effective for the growth rate of crossbreeds are considered to be boars of Landraces and Yorkshires breeds (p<0.05-<0.001). Forage expenditure on the weight gain decreased only reproductors of Landraces breed (p<0.001). Crossbreeding led to the decreased amount of fat in LW pigs behind the terminal rib by 6.76-8.16 mm (p<0.01-<0.001), 6.7-8.44 cm increased the area of cross-section of the longest dorsal muscle (p<0.001) and by 2.83-4.2 % increased the muscularity of the carcass (p<0.01->0.05). The studied boars of the imported breeds also affected some meat quality parameters of Lithuanian White pigs. Drip loss of the crossbreeds of Yorkshires and Large White breed tended to be by 2.89-3.2 % lower than in Lithuanian White pigs (p<0.05-<0.001). The meat of all crossbreds tended to be harder than in Lithuanian White pigs (p<0.05-<0.01) and the tendency of lower water coherence capability (p<0.001->0.05) was also observed. The effect of crossbreeding on the other quality parameters in pigs was less considerable.

scholarly journals СOMBINATIONAL ABILITY OF SPECIALIZED BREEDS AND TYPES OF PIGS IN INDUSTRIAL CROSSBREEDING

2017 ◽  
Vol 53 ◽  
pp. 84-90 ◽  
Author(s):  
O. Vashchenko
Keyword(s):  
Meat Quality ◽  
Combining Ability ◽  
Live Weight ◽  
Individual Species ◽  
Large White ◽  
Large White Breed ◽  
Wide Range ◽  
White Breed ◽  
Conventional Methods

For several decades, widespread industrial crossing different breeds of pigs. However, intensive importation foreign breeding of pig and use foreign technologies, a prerequisite for improving meat and slaughter quality of livestock. Success in solving this problem depends on identifying the best combinations of parental pairs with a comprehensive study of regularities succession and the descendants of preferred breeding traits are determined by genes and polymer characterized by a wide range of variability influenced by environmental conditions. In terms of industrial use of the most successful combinations of lines, genotypes, even a slight increase productivity, ensure, ultimately, significant economic effects. The aim of our study was to evaluate the general (GCA) and specific (SCA) combining ability fattening and meat quality of pigs and calves landed determine the best options for combinations of parental pairs when crossed. Studies were conducted in conditions of industrial technology at without belt dependent concentrations type of feeding and regulated microclimate on the basis of LLB "Mayak-Agro" Cherkassy region. Groups breeder was formed by unique origin, age, physiological condition, body weight. Assessment fattening, meat quality, physical and chemical properties of meat and fat conducted by conventional methods Polivoda AM and else. The morphological composition of carcasses studied by diking corps. The chemical composition and physical properties of meat were determined by conventional methods in condition of biotechnology laboratories Cherkassy National University. B. Khmelnitsky. In the first stage crossed sows of Ukrainian large white breed (UVB) with boars Landrace (LA) English selection, and received from them hybrids (hybrids F1) sires with specialized meat breeds: Pietrain (P) British breeding red white girdle (RWG) and Duroc Ukrainian selection “Steppes” (DUSS). It is established that the use of these industrial crossbreeding schemes leading to improved slaughter yield of 4.4 - 8.4% in the carcasses of local groups. Severity of heterosis for slaughter relies was in the range of 0.16 to 1.88%. Combining breeds LA and UVB has increased the length of the carcasses in hybrids by 2.5 cm (P> 0.95) at the lowest standard deviation - 0.14 cm. At the same time, crossing two-hybrids local pig breeds of boars’ P led rather to reduce the carcasses length compared to the original parental forms 2.6 cm (P> 0.95). Regarding mass index posterior third corps tends to increase it to 11.3 - 12.5 kg accurate advantage of using Landrace breeds (P> 0.95) and pietrain (P> 0.999). The fact that the carcasses obtained from pigs combination 1/4UVB x 1/4LA x 1/2P, were significantly shorter and had the largest area "muscle eye" is quite natural and due to the influence of breeds belonging boars. The efficacy of the combination of maternal two-breeds form hybrids with UVB × LA boars pietrain and red white girdle, confirmed the calculated values of general and specific combining ability. When using boars breed red white girdle highest positive effect of general combining ability was observed in terms of area "muscle eye" (4.94) meeting of body (0.93) slaughter yield (0.35). In addition, the use of the scheme for industrial crossing red white girdle helped reduce fat content in carcass and thick bacon (- 1.41 - 0.64). Increasing the length of the carcass at slaughter hogs promoted the use of Duroc Ukrainian selection “Steppes” (1.32) and Landrace (1.08). Boars used breeds influenced fattening and greasy meat quality of offspring with different force. Its value depended on the individual animal and breeds consolidation for the identified - from negative 8.49 to positive 6.71% age achieve live weight of 100 kg and from minus 9.34 to positive 6.34% in the average daily increments. Differential power of selective effects on offspring among individual species ranged from (- 9.34) to (+6,71)% (P> 0,999). Given the importance of meat quality indicators as signs core products finals pig should always check the structural elements of Ukrainian large white breed on the matching ability. This lets use the best combination to improve performance and avoid unwanted flocks crossbreeds combinations.

Association between leptin receptor gene polymorphism and quality of both meat and back fat in large white pigs of ukrainian breeding

2016 ◽  
Vol 3 (2) ◽  
pp. 42-48
Author(s):  
V. Balatsky ◽  
I. Bankovska ◽  
A. Saienko
Keyword(s):  
Gene Polymorphism ◽  
Leptin Receptor ◽  
Receptor Gene ◽  
Intramuscular Fat ◽  
Large White ◽  
Pig Breeds ◽  
Lepr Gene ◽  
Large White Breed ◽  
White Breed

Leptin receptor is one of the components of the system of regulating energy homeostasis of the organism. Leptin receptor gene (LEPR) polymorphism is associated with pig carcass index of the content of intramus- cular fat in its valuable parts, which is particularly important when assessing the quality of their carcasses for processing. Intramuscular fat is associated with meat fl avor characteristics and partly determines its tenderness, juiciness, and other parameters. Aim. To analyze LEPR gene (SNP NM001024587.1, p. 1987 C > T) polymor- phism in populations of various pig breeds and to establish its relationship with the quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. Methods. Genetic-population analysis of nine pig breeds, associative analysis on the search connection of LEPR gene polymorphism with quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. LEPR locus genotyping was performed by High Resolution Melting (HRM). Results. All the studied breeds are characterized by polymorphism of the leptin receptor gene (SNP NM001024587.1, p. 1987 C > T), signifi cant breed specifi city in the distribution of frequencies of alleles was established. Statistically confi rmed effect (p < 0.05) of genotypes LEPR on the content of intramuscular fat, total dry matter and moisture in the meat, as well as the moisture content in the back fat of pigs of Ukrainian Large White breed was revealed. Higher content of intramuscular fat was found in the animals with genotype TT, while a smaller amount of intramuscular fat and more moisture in fat was revealed in heterozygotes. Conclusions. Genetic marker LEPR SNP NM001024587.1, p. 1987 C > T can be used in the marker-assisted selection to predict and improve the performance quality of the meat of pigs of Large White breed of the Ukrainian breeding. These results suggest that porcine leptin receptor gene controls the quality of fat comp- lex – inside muscles and in the dorsal part of the carcass.

Indexes of reproductive ability and their repeatability in sows of different genotypes, taking into account the polymorphism g.1426g> a of the Mc4r gene

2020 ◽  
pp. 49-62 ◽  
Author(s):  
Viktor Khalak ◽  
Vasyl Voloshchuk ◽  
Konstiantyn Pochernyaev ◽  
Serhii Smyslov ◽  
Maria Ilchenko
Keyword(s):  
Receptor Gene ◽  
Live Weight ◽  
Multiple Births ◽  
Large White ◽  
Mc4r Gene ◽  
Litter Weight ◽  
Reproductive Ability ◽  
Large White Breed ◽  
White Breed ◽  
Consolidation Coefficient

The results of studies of the reproductive ability of sows of different genotypes are given taking into account the polymorphism g.1426G> A of the MC4R gene, the factor of repeatability of characters is determined, and the economic efficiency of the research results is calculated. The experimental part of the research was carried out in the conditions of the «Druzhba-Kaznacheevka» LTD in Dnipropetrovsk region. The objects of research were sows of the Large White breed. It was determined that sows of the main herd, according to signs of reproductive ability, belong to class I and class elite. The experiment showed that sows of genotype AG exceeded their peers of others (GG and AA) in terms of “born piglets total, heads” by 1.6 and 0.7 heads, “Live piglets born (multiple births), heads - by 1 6 and 0.9 heads, “the mass of the litter at the time of birth, kg” - by 2.7 - 1.3 kg., “the mass of the litter at the time of weaning at the age of 28-35 days, kg” - by 5, 1 - 3.9 kg. The difference between animals of the indicated genotypes is: for large-foetus - 0.06-0.01 kg, the index "evenness of the sow’s litter in live weight of piglets at birth, points" - 1.05-1.84 points, index of N. D. Berezovsky - 3.75 -2.36 points. The index of the safety of piglets before weaning ranged from 84.4 to 93.0 %. It was proved that the minimum value of the index “evenness of the sow’s littar in live weight of piglets at the time of birth, points (7.76 ± 1,082 points) are characterized by sows of genotype AA, in which the rate of multiple pregnancy ranged from 8 to 14 pigs, and the value of the sign“ large-foetus, kg "equaled 1.37 kg. The repeatability coefficient for sows of the main herd and sows of the Large White breed of different genotypes for the melanocortin 4 (MC4R) receptor gene ranges from -0.916 ± 0.1418 (І-IV farrow, genotype - GG, sign - “piglet safety before weaning, %) to 0.978 ± 0.0738 (I-V farrow, genotype - GG, sign - “large foetus”, kg ”). The maximum value of the phenotypic consolidation coefficient was found in sows of genotype AA according to the following quantitative characteristics: “live piglets (multiple births), heads.”, “litter weight at the time of birth, kg”, “litter weight at weaning age of 28-35 days, kg "," the safety of piglets before weaning ,% ". The sows of the genotype GG belong to the unconsolidated group according to the signs of reproductive qualities. The use of sows of the genotype for the melanocortin 4 (MC4R) AG receptor gene provides additional products at the level of 5.15 %, and its cost is 165.41 UAH /head. Keywords: sow, breed, reproductive ability, MC4R gene, genotypic consolidation coefficient, variability, correlation, repeatability.

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