Evaluation of haploid diversity of sows of the Large White breed of the pedigree factory of the State enterprise “Experimental enterprise “imeni 9 Sіchnja”

2020 ◽  
pp. 68-73
Author(s):  
Ruslan Hodyna ◽  
Valeriia Matiiuk ◽  
Tetiana Buslyk ◽  
Konstiantyn Pochernyaev
Keyword(s):  
Rflp Analysis ◽  
Ion Exchange Resin ◽  
The State ◽  
State Enterprise ◽  
Production Environment ◽  
Large White ◽  
Mitochondrial Haplotypes ◽  
Large White Breed ◽  
Pcr Rflp ◽  
White Breed

The development and use of effective selection programs accelerated the genetic improvement of breeds, advances in feeding technology allowed for optimal nutrition, and the creation of identical and strictly controlled production conditions, a uniform and strictly controlled production environment led to the replacement of local pig breeds with highly productive transboundary breeds. This development of the industry has led to increased concern about the erosion of genetic resources. The number of purebred boars of the Large White breed in 1973 amounted to 98.3 % of the total number of boars of this breed, and sows - 38.9 %. These data allow us to assume that significant haploid diversity could persist in the individual pedigree factory of the Large White breed. Thus, the subject of our study was the assessment of the haploid diversity of the Large White sows of the pedigree factory of the State enterprise “Experimental enterprise “imeni 9 Sіchnja”, the Institute of Pig Breeding and AIP NAAS of Ukraine. For the study, samples of the bristles of 25 sows of the Large White breed of the SE "Experimental enterprise "imeni 9 Sіchnja" of the Institute of Pig Breeding and AIP NAAS of Ukraine, representing all families. DNA isolation from bristles was performed using a Chelex-100 ion exchange resin. Mitochondrial haplotypes were determined using PCR-RFLP analysis according K. F. Pochernyaev (2016). Among the sample of breeding pigs of the Large White breed of the SE “Experimental enterprise “imeni 9 Sіchnja”, mitochondrial haplotypes B1, B2, C, G, and J1 were identified. In previous studies, seven mitochondrial haplotypes were found among pigs of the Large White breed of Ukraine, which were found with different frequencies. The haplotypes G (14.1 %), J1 (13.3 %) and N (5.91 %) were found with the highest frequency; much less often - haplotypes A (0.3 %), B1 (1.9 %), C (9.2 %) and L (2.2 %). Thus, our studies have shown that the mitochondrial haplotypes B1, B2, C, G, J1 found in the sample of breeding pigs of the Large White breed of the SE «Experimental enterprise «imeni 9 Sіchnja» typical for pigs of the Large White breed of Ukraine except haplotype B2. Important for the restoration of the Mirgorod breed of pigs is the existence in the herd of pigs of the Large White breed of the SE “Experimental entewrprise“imeni 9 Sіchnja” animals having haplotype B1 inherent to Myrgorod breed. Thus, our studies have shown that in sows of the Large White breed breeding farm SE “Experimental enterprise “imeni 9 Sіchnja”, significant haploid diversity remains, which must be maintained at a high level in the future. Key words: pigs, the Large White breed, haplotype, mitochondrial genome, PCR-RFLP.

scholarly journals Association of LEP- and CTSF-genotypes with levels of meat quality PSE, NOR and DFD in pigs of large white breed of Ukrainian selection

2020 ◽  
Vol 7 (1) ◽  
pp. 14-23
Author(s):  
I. Bankovska ◽  
Y. Oliinychenko ◽  
V. Balatsky ◽  
T. Buslyk ◽  
S. Hryshchenko ◽  
...  
Keyword(s):  
Meat Quality ◽  
Genetic Markers ◽  
Population Analysis ◽  
Dna Typing ◽  
Rflp Analysis ◽  
Large White ◽  
Genetic Population ◽  
Large White Breed ◽  
White Breed ◽  
Meat Samples

Aim. To study the distribution of genotypes by polymorphisms of the leptin (LEP SNP g.3469 T > C, LEP SNP g.2845 A > T) and cathepsin F (CTSF SNP g.22 C > G) genes according to the quality levels PSE, NOR and DFD meat in Large White pig breed of Ukrainian selection and to fi nd associations of genetic markers with the total indicator of meat quality. Methods. We studied meat samples (m. longissimus dorsi) from 102 pigs of Ukrainian Large White breed, raised to the weight at slaughter of 120 ± 5 kg in the experimental farm “Stepne” of the Poltava region, Ukraine. The ranking of muscle tissue was carried out according to the total indicator of meat quality (TM). PCR-RFLP analysis was used for DNA typing. The associations between genetic markers and TM were determined using ANOVA. Results. Genetic population analysis of Large White breed pigs of Ukrainian selection by genetic markers LEP SNP g.3469 T > C, LEP SNP g.2845 A > T and СTSF SNP g.22 C > G was carried out. The informative value of LEP SNP g.2845 A > T and СTSF SNP g.22 C > G, according to the calculated polymorphism information content, was optimal for associative studies (PIC = 0.311 and 0.373, respectively). The distribution of meat samples by quality levels PSE, NOR and DFD was performed. Most of them had traits of moderately expressed (n = 22) and weakly expressed (n = 59) PSE defect (light, soft, exudative meat). The calculated coeffi cients of Chuprov’s mutual conjugation between the genotypes for the studied SNPs and meat quality levels showed a moderate relationship between the genotypes for LEP SNP g.2845 A > T and CTSF SNP g.22 C > G and meat quality levels, К = 0.26 and 0.24, respectively. According to the results of ANOVA, the differences were found between homozygous and heterozygous CTSF SNP g.22 C > G genotypes in terms of the total indicator of meat quality. Conclusions. The meat of heterozygous pigs for CTSF SNP g.22 C > G (g.22GC) is characterized by a higher total indicator of meat quality (4.6) compared to the meat of homozygous animals g.22GG (4.2, p ≤ 0.05) and g.22CC (3.9, p ≤ 0.01).

Association between leptin receptor gene polymorphism and quality of both meat and back fat in large white pigs of ukrainian breeding

2016 ◽  
Vol 3 (2) ◽  
pp. 42-48
Author(s):  
V. Balatsky ◽  
I. Bankovska ◽  
A. Saienko
Keyword(s):  
Gene Polymorphism ◽  
Leptin Receptor ◽  
Receptor Gene ◽  
Intramuscular Fat ◽  
Large White ◽  
Pig Breeds ◽  
Lepr Gene ◽  
Large White Breed ◽  
White Breed

Leptin receptor is one of the components of the system of regulating energy homeostasis of the organism. Leptin receptor gene (LEPR) polymorphism is associated with pig carcass index of the content of intramus- cular fat in its valuable parts, which is particularly important when assessing the quality of their carcasses for processing. Intramuscular fat is associated with meat fl avor characteristics and partly determines its tenderness, juiciness, and other parameters. Aim. To analyze LEPR gene (SNP NM001024587.1, p. 1987 C > T) polymor- phism in populations of various pig breeds and to establish its relationship with the quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. Methods. Genetic-population analysis of nine pig breeds, associative analysis on the search connection of LEPR gene polymorphism with quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. LEPR locus genotyping was performed by High Resolution Melting (HRM). Results. All the studied breeds are characterized by polymorphism of the leptin receptor gene (SNP NM001024587.1, p. 1987 C > T), signifi cant breed specifi city in the distribution of frequencies of alleles was established. Statistically confi rmed effect (p < 0.05) of genotypes LEPR on the content of intramuscular fat, total dry matter and moisture in the meat, as well as the moisture content in the back fat of pigs of Ukrainian Large White breed was revealed. Higher content of intramuscular fat was found in the animals with genotype TT, while a smaller amount of intramuscular fat and more moisture in fat was revealed in heterozygotes. Conclusions. Genetic marker LEPR SNP NM001024587.1, p. 1987 C > T can be used in the marker-assisted selection to predict and improve the performance quality of the meat of pigs of Large White breed of the Ukrainian breeding. These results suggest that porcine leptin receptor gene controls the quality of fat comp- lex – inside muscles and in the dorsal part of the carcass.

Indexes of reproductive ability and their repeatability in sows of different genotypes, taking into account the polymorphism g.1426g> a of the Mc4r gene

2020 ◽  
pp. 49-62 ◽  
Author(s):  
Viktor Khalak ◽  
Vasyl Voloshchuk ◽  
Konstiantyn Pochernyaev ◽  
Serhii Smyslov ◽  
Maria Ilchenko
Keyword(s):  
Receptor Gene ◽  
Live Weight ◽  
Multiple Births ◽  
Large White ◽  
Mc4r Gene ◽  
Litter Weight ◽  
Reproductive Ability ◽  
Large White Breed ◽  
White Breed ◽  
Consolidation Coefficient

The results of studies of the reproductive ability of sows of different genotypes are given taking into account the polymorphism g.1426G> A of the MC4R gene, the factor of repeatability of characters is determined, and the economic efficiency of the research results is calculated. The experimental part of the research was carried out in the conditions of the «Druzhba-Kaznacheevka» LTD in Dnipropetrovsk region. The objects of research were sows of the Large White breed. It was determined that sows of the main herd, according to signs of reproductive ability, belong to class I and class elite. The experiment showed that sows of genotype AG exceeded their peers of others (GG and AA) in terms of “born piglets total, heads” by 1.6 and 0.7 heads, “Live piglets born (multiple births), heads - by 1 6 and 0.9 heads, “the mass of the litter at the time of birth, kg” - by 2.7 - 1.3 kg., “the mass of the litter at the time of weaning at the age of 28-35 days, kg” - by 5, 1 - 3.9 kg. The difference between animals of the indicated genotypes is: for large-foetus - 0.06-0.01 kg, the index "evenness of the sow’s litter in live weight of piglets at birth, points" - 1.05-1.84 points, index of N. D. Berezovsky - 3.75 -2.36 points. The index of the safety of piglets before weaning ranged from 84.4 to 93.0 %. It was proved that the minimum value of the index “evenness of the sow’s littar in live weight of piglets at the time of birth, points (7.76 ± 1,082 points) are characterized by sows of genotype AA, in which the rate of multiple pregnancy ranged from 8 to 14 pigs, and the value of the sign“ large-foetus, kg "equaled 1.37 kg. The repeatability coefficient for sows of the main herd and sows of the Large White breed of different genotypes for the melanocortin 4 (MC4R) receptor gene ranges from -0.916 ± 0.1418 (І-IV farrow, genotype - GG, sign - “piglet safety before weaning, %) to 0.978 ± 0.0738 (I-V farrow, genotype - GG, sign - “large foetus”, kg ”). The maximum value of the phenotypic consolidation coefficient was found in sows of genotype AA according to the following quantitative characteristics: “live piglets (multiple births), heads.”, “litter weight at the time of birth, kg”, “litter weight at weaning age of 28-35 days, kg "," the safety of piglets before weaning ,% ". The sows of the genotype GG belong to the unconsolidated group according to the signs of reproductive qualities. The use of sows of the genotype for the melanocortin 4 (MC4R) AG receptor gene provides additional products at the level of 5.15 %, and its cost is 165.41 UAH /head. Keywords: sow, breed, reproductive ability, MC4R gene, genotypic consolidation coefficient, variability, correlation, repeatability.

scholarly journals Hybridization as a method of improving quality of pork

2020 ◽  
pp. 31-35
Author(s):  
B. P. Kovalenko ◽  
◽  
O. B. Shevchenko ◽  
Keyword(s):  
Chemical Composition ◽  
Physical Properties ◽  
Ph Value ◽  
Longissimus Dorsi ◽  
Large White ◽  
Group Iii ◽  
Large White Breed ◽  
White Breed ◽  
A Share

Studies were carried out on the main indicators of the chemical composition and physical properties of muskulus longissimus dorsi of pigs of different genotypes during hybridization. The indicators of the chemical composition and physical properties of muskulus longissimus dorsi were determined by conventional methods. The materials of experimental studies were processed using methods of variation statistics. According to the amount of moisture, no significant difference between the control and experimental groups was established. According to the amount of protein in meat, a tendency to a slight decrease, compared with the control group by 1.4 (group III) … 1.9 % (group II) was established. The use of boars of Poltava meat breed led to a decrease in intramuscular fat in descendants by 0.25 % compared with peers of a large white breed (P <0.95), and precocious meat - to a real increase of this indicator by 0.43 % at P> 0 , 95. A tendency towards an increase in the amount of tryptophan (by 0.04 ... 0.05) and oxyproline (by 0.004 ... 0.005) in the muscles of hybrid animals was revealed in comparison with peers of pure breed of large white breed. In terms of the biological usefulness of meat proteins, animals of group III were the best - in terms of the quality of the protein of the longest muscle of the back, their advantage over peers of group II was 0.07, and compared with purebred peers. Group I - 0.12 (p <0.95). It was found that with the same area of the meat spot both in purebred large white breeds of animals and hydrides of different genotypes, there is a tendency to increase the latter compared to the control in a humid place: with a «share» of «blood» ½ pm by 2.2 %, and with a «share» of blood ½ cm - 1.4 %. A tendency has been established to increase the pH value of the longest back muscle in animals with a «share» of blood of specialized meat breeds compared with a large white breed. In hybrid animals with a «share» of blood of Poltava meat and precocious meat breeds, there is a tendency to increase the pH value in comparison with the large white breed by 0.15 (2.8 %) and by 0.17 (3.1 %), i.e. the meat of hybrid pigs, the pH of which has a shift towards alkalinity due to less accumulation of lactic acid, has a worse preservation.

scholarly journals ЗВ'ЯЗОК G.143C>T ПОЛІМОРФІЗМУ ГЕНА CTSL ІЗ ВІДГОДІВЕЛЬНИМИ І М’ЯСНИМИ ЯКОСТЯМИ СВИНЕЙ ВЕЛИКОЇ БІЛОЇ ПОРОДИ УКРАЇНИ

2014 ◽  
pp. 88-90
Author(s):  
К. Ф. Почерняєв ◽  
В. М. Балацький ◽  
І. К. Лядський
Keyword(s):  
Impact Force ◽  
Large White ◽  
Meat Yield ◽  
Large White Breed ◽  
White Breed ◽  
Different Populations ◽  
The Impact

Викладені результати досліджень зв’язку гена ctslіз відгодівельними і м’ясними якостями свиней вели-кої білої породи України. Проаналізовано особливостіалелофонду гена ctsl у різних популяціях свиней ВБпороди; проведено аналіз частот і розповсюдженнярізних генотипів; вивчено зв'язок g.143C>T полімор-фізму цього гена з товщиною відкладення сала посередині спини та в ділянці 6–7-го ребер, а також ізсередньодобовими приростами,товщиною найдовшо-го м’яза спини й виходом м’яса. Визначено силу впли-ву g.143C>T поліморфізму гена ctsl на ці ознаки.Встановлено достовірні відмінності (P<0,05) за де-якими з вищезгаданих ознак у свиней із різними гено-типами гена ctsl. На підставі проведених дослідженьзроблені висновки та подані пропозиції виробництву. The results of researches of the association of gene ctsl   with the formation of fattening and meat qualities in pigs of the Large White breed of Ukraine are given. It has been analysed the features of gene ctsl alelofund in different populations of pigs of LW breed, it was carried out the analysis of frequencies and distribution of different genotypes. The association g.143C>T polymorphism of this gene with thick deposits of fat in the middle of the back and in area of 6-7 ribs, as well as average daily gains , thickness of the longest muscles and meat yield was studied. It has been determined the impact force g.143C>T polymorphism of gene ctsl on these traits. It was found out significant differences (P <0.05) for some of the above traits in pigs with different genotypes of gene ctsl. On the basis of the researches it was made conclusions and advanced proposals to producers.

scholarly journals EFFICIENCY OF INTRAUTERINE INSEMINATION IN BREEDING SOWS

2017 ◽  
Vol 53 ◽  
pp. 254-259
Author(s):  
V. O. Melnik ◽  
O. O. Kravchenko ◽  
О. S. Kohut
Keyword(s):  
Artificial Insemination ◽  
Intrauterine Insemination ◽  
Live Weight ◽  
Reproductive Technologies ◽  
Breeding Value ◽  
Large White ◽  
Large White Breed ◽  
Significant Difference ◽  
White Breed ◽  
Breeding Farms

Improving of fertility indicators and reproductive qualities of sows during artificial insemination in farms of different specializations is very topical issue. The introduction of artificial insemination of sows on breeding farms by the spermdoses of optimum volume, by the frozen-thawed and sex sperm requires the introduction of innovative reproductive technologies. The use of economical methods of artificial insemination of sows using a minimum number of sperm in a small volume of spermdose in order to achieve high rates of fertility and prolificacy was proven in numerous experiments of the authors. Significantly reduced spermdose may be sufficient if the sperm enters deep enough into the uterus. Vitality of sperm does not depend on the size of spermdose, but the best place for sperm to survive one oviducts where they keep the fertilizing capacity from 9 to 27 hours. So deep intrauterine insemination of sows improves conditions for sperm survival The aim was to study the feasibility and justification for widespread implementation in to production on breeding farms of intrauterine insemination of sows morder to increase their fertilization and prolificacy and  to save the boar sperm with the highest index of breeding values. Experiments were conducted in terms of selection and genetic center of Agrofirm "Mig-Service-Agro" in Mykolaiv region. In the experiment used 65 sows of live weight of 280-320 kg wiht 2-4 farrowing were. Sows in sexual hunting were showed once daily in the morning using a boar-prober. Artificial insemination was performed twice: the first time - in the afternoon and at 14-16 p.m. The second time – in the morning of the next day at 9-10 am. For artificial insemination of sows were ed using experimental spermodes with volume of 40 ml which contained 1.5 billion of a Active sperm. For the dilution of sperm was used Durasperm - KRUUSE (Denmark) the period of sperm perpetuation is 5-7 days. To enter the were semen used catheters Magaplus S, (Spain) for intrauterine insemination of sows. Analysis shows that the period from weaning to insemination has significant difference comparing sows of large White breed with Landrace breed (p <0.001), with genotype sows F1 (p <0.01) and sows of the Duroc breed (p <0.05). For all selected 65 sows duration of suckling period, was estimated which averaged 32.2 days and the average time from weaning of pigs to their sexual inclination and the first intrauterine insemination 6.8 days that meets the physiological norm. After intrauterine insemination of sows of then 48 farrowed, which wich made for 73.9%. іncluding live 5 emergency farrow  were obtained, representing 10.4% of all amount. Percentage of farrow is considered physiologically normal – 80%, or more of total insemined sows. The very low percentage of farrow 53.3% had of sows F1, and the highest percentage was found by sows of the Duroc breed – 85.7%. Pregnency of sows were received just 17, which made 26.1% and highest percentage – 46.7% was set by sows F1. Analysis of the pregnancy sows shows that on average it is 116.2 days was the longest – 117.1 days was set in Landrace breed sows and the short est 115.5 days in Large White breed, but the difference is not significant. 571 pigs were received, іncluding live 451 head, which is 78.9%. The largest percentage of іncluding live piglets obtained from sows F1 – 82.5%, and the lowest in Landrace breeds – 77.0% and Large White – 77.3%. Exit of all piglets per sow without emergency farrowings is 12.2, іncluding live – 9,8. The highest yield were obtained piglets from sows F1 – 13.1, іncluding live – 10.9, the lowest yield of sows of the Duroc breed – 10.6, іncluding live 9.1, which has significant difference compared with the control (IDPs) and other breeds.  After intrauterine insemination 8 sows showed cyclic deregulation in 20-25 days, ie repeated sexual hunt took place on average 22.3 days. These sows were inseminated by not fractional way, they farrowed and an average litter just 13.1 piglets per sow, іncluding live - 11.3 was obtained. Repeating after intrauterine insemination on 45-48-49 day in the sexual hunt came about three sows for artificial insemination by not fractional method 3 farrowed and was obtained output – 13.3 piglets, іncluding live – 11.7. It should be noted that the best sow Large White breed №12 after intrauterine insemination bore 16 pigs, іncluding live 11, sow of Landrace breed №1556 – 18 pigs, іncluding live 12, Duroc №5888 – 13 pigs, іncluding live 11, sow F1 №167 – 20 pigs, іncluding live 14 pigs. Breeding requires more careful handling with major sows taking into account their breeding value and cost, that’s why we believe that there is no need to risk causing injury genitals with intrauterine insemination if a sufficient number of spermdoses of boars-sires exist.

scholarly journals Analysis of prolificacy in sows of hyperprolific lines of Large White breed

2011 ◽  
Vol 50 (No. 4) ◽  
pp. 155-162
Author(s):  
V. Matoušek ◽  
A. Čermáková ◽  
N. Kernerová ◽  
P. Králová
Keyword(s):  
Candidate Genes ◽  
Reproductive Performance ◽  
Reproductive Traits ◽  
Breeding Value ◽  
Large White ◽  
Large White Breed ◽  
Lifetime Performance ◽  
White Breed ◽  
Positive Effect ◽  
Esr Gene

The objective of the paper was to evaluate the results of reproductive performance of sows in some elite breeding herds of the Large White breed included in experimental herds for the production of hyperprolific lines of dam breeds. The set consisted of 98 sows with the known genotypes of ESR, FSH&acirc; and PRLR genes. The dendrogram shows that on the basis of their genetic outfit the sows can be divided into two clusters. The first cluster can be described as a cluster with marked dominance of HPL sows and the second cluster with marked dominance of the sows of basic herd. The first cluster consisted of individuals in which the preferred genotype AA of PRLR gene was not detected. As for FSH&acirc; gene, the beneficial genotype BB was found out in 74.10% of sows. In ESR gene the beneficial genotype DD was recorded only in 11.10% of individuals. On average for the lifetime performance they delivered by 2.08 piglets more in all born piglets and by 1.96 piglets more in live-born piglets per litter. Differences in the reproductive traits between HPL sows and the sows of basic herd in the first cluster were statistically highly significant. On the contrary, genotype AA of PRLR gene was identified in all sows of the second cluster, 61.36% of animals possessed beneficial genotype BB of FSH&acirc; gene. As for ESR gene, beneficial genotype DD was identified within the whole cluster in 31.82% of sows. In lifetime performance the HPL sows had on average by 1.10 individuals more in all born piglets and by 1.01 more in live-born piglets (statistically significantly higher values). The unambiguous expression of a positive effect of preferred genotypes of selected candidate genes failed to be confirmed by the results of statistical analyses testing the associations of candidate genes for pig reproduction with selected parameters of breeding value and prolificacy of sows. &nbsp;

Culture-dependent diversity profiling of spoilage yeasts species by PCR-RFLP comparative analysis

2019 ◽  
Vol 25 (8) ◽  
pp. 671-679
Author(s):  
Kereng M Corbett ◽  
Olga de Smidt
Keyword(s):  
Comparative Analysis ◽  
Rflp Analysis ◽  
Action Planning ◽  
Economic Losses ◽  
Process Equipment ◽  
Small Scale ◽  
Control Panel ◽  
Production Environment ◽  
Spoilage Yeasts ◽  
Pcr Rflp

Spoilage caused by yeasts is a constant, widespread problem in the beverage industry that can result in major economic losses. Fruit juices provide an environment that allows the proliferation of yeast. Some factories in South Africa are not equipped with laboratory facilities to identify spoilage yeasts and outsourcing becomes a prolonged process which obstructs corrective action planning. This study aimed to establish yeast diversity and apply a rapid method for preliminary identification of spoilage yeasts associated with a small-scale fruit juice bottling factory. Yeast population in the factory was determined by isolation from the production environment, process equipment and spoiled products. PCR-RFLP analysis targeting the 5.8S-ITS region and D1/D2 sequencing was used for identification. A total of 207 yeasts belonging to 10 different genera ( Candida, Lodderomyces, Wickerhamomyces, Yarrowia, Zygosaccharomyces, Zygoascus, Cryptococcus, Filobasidium, Rhodotorula/Cystobasidium and Trichosporon) were isolated and identified from the production environment and processing equipment. Candida intermedia, C. parapsilosis and Lodderomyces elongisporus were widely distributed in the factory. Zygosaccharomyces bailii, Z. bisporus, Zygoascus hellenicus and Saccharomyces cerevisiae were isolated from the spoiled products. The data provided a yeast control panel that was used successfully to identify unknown yeasts in spoiled products from this factory using polymerase chain reaction-restriction length polymorphism (PCR-RFLP) comparative analysis.

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