STATUS OF INDICATORS OF HUMORAL LINK OF NATURAL IMMUNITY IN RATS UNDER OXIDATIVE STRESS AND ACTION OF LIPOSOMAL PREPARATION

The article gives the results of the research on the influence of a developed complex liposomal preparation on the dynamics of morphological and biochemical indicators of the blood of rats subjected to modeled oxidative stress caused by intermuscular injection of 50% carbon tetrachloride at a dose of 0.25 ml per 100 g of body mass. We found that in conditions of intoxication by carbon tetrachloride, the physiological level of hematological indicators of the researched animals’ body was disrupted. This is indicated by the reduction in the amount of erythrocytes, hemoglobin content, concentration of hemoglobin per erythrocyte, increase in the amount of leucocytes, mass of hemoglobin per erythrocyte and increase of colour indicator. Also, we detected suppression of protein synthesis function of the liver. The levels of total protein and albumin fraction were below the normal physiological level. High indicators were observed in the levels of creatinine, urea and total bilirubin. In order to normalize the functional state of the liver under oxidative stress it is reasonable to use a liposomal preparation which contains butafosfan, interferon, thistle and vitamins. In order to normalize morphological and biochemical indicators of the blood of rats subject to intoxication of carbon tetrachloride it is reasonable to use a liposomal preparation that contains butafosfan, selenium, thistle, methionine and vitamins. Using the liposomal preparation Butaselmevit for rats under oxidative stress the morphological and biochemical indicators in the blood normalized. On the 14th day the indictors of the number of erythrocytes, the hemoglobin content, the white blood cell count and red blood indexes compared to control came within the physiological indicators, indicating a recovery of hematopoietic function of the bone marrow and normalized indicators of the functional state of the liver.

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Activity of the T- and B-system of the cell immunity of animals under conditions of oxidation stress and effects of the liposomal drug

Ukrainian Journal of Ecology ◽

10.15421/2017_157 ◽

2017 ◽

Vol 7 (4) ◽

pp. 536-541 ◽

Cited By ~ 3

Author(s):

M. Khariv ◽

B. Gutyj ◽

N. Ohorodnyuk ◽

O. Vishchur ◽

I. Khariv ◽

...

Keyword(s):

Oxidative Stress ◽

T Lymphocytes ◽

B Lymphocytes ◽

Cellular Immunity ◽

Relative Number ◽

The Body ◽

T And B Lymphocytes ◽

Oxidation Stress ◽

Liposomal Preparation ◽

Experimental Group

The results of research on the influence of the elaborated complex immunotropic drug containing butafosfan, interferon, thistle and fat-soluble vitamins A, D3, E in the form of a liposomal emulsion on the activity of T- and B-cell chains of immunity in rats under the conditions of action on the body of oxidative stress are presented. It has been established that the introduction of 50% tetrachloromethane into the rats of the first and second experimental groups, with a dose of 0.25 ml per 100 g of body weight, causes oxidative stress in them which negatively affects the cellular immunity and functional activity of immunocompetent blood cells. Immunosuppressive effects of oxidative stress were manifested by a decrease in the blood of rats in the first and second experimental groups of the number of T- and B-lymphocytes and their regulatory subpopulations mainly on the 2nd and 5th day of the study. At the same time, in the blood of rats of the first experimental group in all research periods a decrease in the relative number of common, active and theophylline-resistant T-lymphocytes, as well as B-lymphocytes was observed with a noticeable increase in the number of their undifferentiated forms. At the same time, the obtained data suggest the positive effect of butafosfan, interferon, thistle and vitamins A, D3, E in the liposomal preparation on the relative amount of T- and B-lymphocytes and on the redistribution of avidity in the direction of strengthening the receptor field of plasma membranes immunocompetent cells. It was found that the normalization on the 2nd day of blood level in the second experimental group of common T-lymphocytes occurred due to the secondary forms of the blood and active T-lymphocytes by changes in the number of low-avid forms. In addition to the indicators characterizing the cellular immunity of rats, the components of the liposomal preparation showed regulatory influence on the humoral link of the immune response. In particular, on the 10th day of research in blood of rats of the second experimental group a tendency towards an increase in the relative number of B-lymphocytes and an increase in the number of cells with low and medium density of receptors was found, which, under the conditions of oxidative stress, indicates an increase in the body's ability to actively synthesize protective antibodies

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Dynamics of phagocytic activity of neutrophils in rats under oxidative stress and the action of liposomal preparation

The Animal Biology ◽

10.15407/animbiol19.01.119 ◽

2017 ◽

Vol 19 (1) ◽

pp. 119-124

Author(s):

M. Khariv ◽

◽

B. Gutyj ◽

Keyword(s):

Oxidative Stress ◽

Phagocytic Activity ◽

Liposomal Preparation

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Indicators of functional and antioxidant liver status of rats under oxidative stress conditions and on the action of the liposomal drug “Butaselmevit”

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.32718/nvlvet8919 ◽

2018 ◽

Vol 20 (89) ◽

pp. 100-107 ◽

Cited By ~ 1

Author(s):

T. V. Martyshuk ◽

B. V. Gutyj ◽

O. I. Vishchur

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Glutathione Peroxidase ◽

Reduced Glutathione ◽

Feed Additives ◽

Male Rats ◽

Intramuscular Administration ◽

Liposomal Drug ◽

Liposomal Preparation ◽

Experimental Group

The purpose of the work was to study the parameters of the functional and antioxidant state of the liver in rats under conditions of oxidative stress and at the actions of the liposomal drug “Butaselmevit”. The research was carried out on young white laboratory male rats of the Vistar line with a body weight of 180-200 g, which were kept in the standard conditions of the institute vivarium of the State Scientific Research Control Institute of Veterinary Preparations and Feed Additives. Animals were divided into three groups of 20 animals in each: 1st group (K) intact animals; 2nd group (D1) – rats, affected with tetrachloromethane; group 3 (D2) – rats, affected with tetrachloromethane and treated with Botselmevit liposomal preparation. Toxic lesion of rats was caused by intramuscular administration of 50% tetrachloromethane at a dose of 0.25 ml per 100 g of animal body weight for the first and third day of the study. Animals of the experimental group D2 on the first and third day of the study, an hour after the introduction of tetrachloroethane, additionally administered a liposomal preparation at a dose of 2 ml per 1 kg of body weight of the animal. The composition of this preparation includes the following substances: butafosfan, selenium, methionine, thistle injection and vitamins A, E and D3. The development of oxidative stress in rats caused by intramuscular administration of tetrachloromethane was accompanied by suppression of protein synthesis of the liver, as evidenced by the low level of total protein and the decrease in the albumin-globulin factor. Low levels of albumin and high levels of globulins in the blood of diseased rats indicate an albumin-globulin disproportion. The indicators of liver function were quite high, namely: the level of creatinine increased by 46%, urea by 74% and total bilirubin by 34%. When applying the liposomal drug "Butaselmevit" to rats, under conditions of oxidative stress during research, the normalization of biochemical parameters occurs in the blood. It has been proven that modeling of the stress response in rats of the experimental group leads to a probable decrease in the level of reduced glutathione and the activity of glutathione peroxidase in the second and fifth days of the experiment. When applying the liposomal drug Butaselmevit to rats, under conditions of oxidative stress during the studies, activation of the glutathione linkage of the antioxidant system occurs in the blood, indicating an increase in the level of reduced glutathione and the activity of glutathione peroxidase in the blood of animal data. At the 14th day of the research, the level of reduced glutathione and the activity of glutathione peroxidase in blood of the experimental group D2 were the highest. The results of the research indicate the antioxidant properties of the new liposomal drug “Butaselmevit”.

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Influence of the liposomal preparation Butaintervite on protein synthesis function in the livers of rats under the influence of carbon tetrachloride poisoning

Visnyk of Dnipropetrovsk University Biology medicine ◽

10.15421/021622 ◽

2016 ◽

Vol 7 (2) ◽

pp. 123-126

Author(s):

M. I. Hariv ◽

B. V. Gutyj

Keyword(s):

Oxidative Stress ◽

Protein Synthesis ◽

Carbon Tetrachloride ◽

Total Protein ◽

The Body ◽

Control Group ◽

Blood Levels ◽

Carbon Tetrachloride Poisoning ◽

Liposomal Preparation ◽

Experimental Group

This article presents the results of research into the influence of the complex liposomal preparation Butaintervit on protein synthesis function in the livers of rats under the influence of carbon tetrachloride poisoning. Intramuscular injection of carbon tetrachloride into rats at a dose of 0.25 ml per100 gof body weight causes antigenic load on the body and leads to disruption of protein synthesis function in the liver. This is shown by reduction in blood levels of total protein and its fractions. Thus, the level of albumin in the serum of rats under the conditions of oxidative stress was 70% lower than in clinically healthy animals. However, the level of total protein in the serum was only 10% lower. This is because, along with the decrease of albumin content in the serum, the levels of globulin protein fraction increased by 8.8%. This has led to albumin/globulin disparities in the serum of sick animals. As a result, the value of A/G coefficient was 0.28 ± 0.03, compared to 0.52 ±0.02 inclinically healthy rats. For the normalization of functional state of the liver under oxidative stress it is advisable to apply the liposomal preparation Butaintervite, which in its structure contains butafosfan, interferon, thistle and vitamins A, D and E. Under conditions of oxidative stress and under the action of the liposomal preparation in the rats from the second experimental group we have found significant increase in the levels of total protein and albumins and a decrease in serum globulin in the animals on the fifth and tenth days of the experiment. On the fourteenth day of the experiment under the conditions of oxidative stress and under the action of the liposomal preparation in the rats from the second experimental group the normalization of protein synthesis function in the liver was observed. The level of indicators of total protein, albumin, globulin and the coefficient of albumin/globulin compared with the control group of animals were within normal values.

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Lycopene alleviates sulfamethoxazole-induced hepatotoxicity in grass carp (Ctenopharyngodon idellus) via suppression of oxidative stress, inflammation and apoptosis

Food & Function ◽

10.1039/d0fo01638a ◽

2020 ◽

Vol 11 (10) ◽

pp. 8547-8559

Author(s):

Hongjing Zhao ◽

Yu Wang ◽

Mengyao Mu ◽

Menghao Guo ◽

Hongxian Yu ◽

...

Keyword(s):

Oxidative Stress ◽

Secondary Metabolites ◽

Human Health ◽

Grass Carp ◽

Aquatic Environment ◽

Ctenopharyngodon Idellus

Antibiotics are used worldwide to treat diseases in humans and other animals; most of them and their secondary metabolites are discharged into the aquatic environment, posing a serious threat to human health.

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Copper-dependent ATP7B up-regulation drives the resistance of TMEM16A-overexpressing head-and-neck cancer models to platinum toxicity

Biochemical Journal ◽

10.1042/bcj20190591 ◽

2019 ◽

Vol 476 (24) ◽

pp. 3705-3719 ◽

Cited By ~ 2

Author(s):

Avani Vyas ◽

Umamaheswar Duvvuri ◽

Kirill Kiselyov

Keyword(s):

Oxidative Stress ◽

Head And Neck ◽

Transcriptional Activation ◽

Platinum Resistance ◽

Mrna Levels ◽

Strong Positive Correlation ◽

Platinum Compounds ◽

Copper Chelation ◽

Novel Approach ◽

Cancer Models

Platinum-containing drugs such as cisplatin and carboplatin are routinely used for the treatment of many solid tumors including squamous cell carcinoma of the head and neck (SCCHN). However, SCCHN resistance to platinum compounds is well documented. The resistance to platinum has been linked to the activity of divalent transporter ATP7B, which pumps platinum from the cytoplasm into lysosomes, decreasing its concentration in the cytoplasm. Several cancer models show increased expression of ATP7B; however, the reason for such an increase is not known. Here we show a strong positive correlation between mRNA levels of TMEM16A and ATP7B in human SCCHN tumors. TMEM16A overexpression and depletion in SCCHN cell lines caused parallel changes in the ATP7B mRNA levels. The ATP7B increase in TMEM16A-overexpressing cells was reversed by suppression of NADPH oxidase 2 (NOX2), by the antioxidant N-Acetyl-Cysteine (NAC) and by copper chelation using cuprizone and bathocuproine sulphonate (BCS). Pretreatment with either chelator significantly increased cisplatin's sensitivity, particularly in the context of TMEM16A overexpression. We propose that increased oxidative stress in TMEM16A-overexpressing cells liberates the chelated copper in the cytoplasm, leading to the transcriptional activation of ATP7B expression. This, in turn, decreases the efficacy of platinum compounds by promoting their vesicular sequestration. We think that such a new explanation of the mechanism of SCCHN tumors’ platinum resistance identifies novel approach to treating these tumors.

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Clinical aspects of reactive oxygen and nitrogen species

Biochemical Society Symposium ◽

10.1042/bss0710121 ◽

2004 ◽

Vol 71 ◽

pp. 121-133 ◽

Cited By ~ 25

Author(s):

Ascan Warnholtz ◽

Maria Wendt ◽

Michael August ◽

Thomas Münzel

Keyword(s):

Oxidative Stress ◽

Reactive Oxygen Species ◽

Risk Factor ◽

Endothelial Dysfunction ◽

Vascular Tissue ◽

Rapid Development ◽

Reactive Oxygen ◽

Clinical Aspects ◽

No Production ◽

Oxygen Species

Endothelial dysfunction in the setting of cardiovascular risk factors, such as hypercholesterolaemia, hypertension, diabetes mellitus and chronic smoking, as well as in the setting of heart failure, has been shown to be at least partly dependent on the production of reactive oxygen species in endothelial and/or smooth muscle cells and the adventitia, and the subsequent decrease in vascular bioavailability of NO. Superoxide-producing enzymes involved in increased oxidative stress within vascular tissue include NAD(P)H-oxidase, xanthine oxidase and endothelial nitric oxide synthase in an uncoupled state. Recent studies indicate that endothelial dysfunction of peripheral and coronary resistance and conductance vessels represents a strong and independent risk factor for future cardiovascular events. Ways to reduce endothelial dysfunction include risk-factor modification and treatment with substances that have been shown to reduce oxidative stress and, simultaneously, to stimulate endothelial NO production, such as inhibitors of angiotensin-converting enzyme or the statins. In contrast, in conditions where increased production of reactive oxygen species, such as superoxide, in vascular tissue is established, treatment with NO, e.g. via administration of nitroglycerin, results in a rapid development of endothelial dysfunction, which may worsen the prognosis in patients with established coronary artery disease.

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