scholarly journals KEANEKARAGAMAN FENOTIPIK NGENGAT (Subordo Heterocera) BERDASARKAN KARAKTER MORFOLOGI DI KAWASAN UNIVERSITAS RIAU DAN DESA SIABU, PROVINSI RIAU

2019 ◽  
Vol 12 (1) ◽  
pp. 25-37
Author(s):  
Ennie Chahyadi ◽  
Nuryani Nuryani ◽  
Dewi Indriyani Roslim
Keyword(s):  
Genetic Diversity ◽  
Light Source ◽  
Genetic Similarity ◽  
Sampling Method ◽  
Secondary Forest ◽  
Light Trap ◽  
Morphological Characters ◽  
The Body ◽  
Science Faculty ◽  
Mathematics And Science

AbstrakPenelitian tentang ngengat masih sangat sedikit dilakukan di Indonesia terutama di kawasan Universitas Riau dan Desa Siabu Kampar belum ada datanya. Penelitian ini bertujuan untuk menganalisis keanekaragaman fenotip ngengat berdasarkan karakter morfologi untuk mengetahui jarak genetik ngengat di kawasan Universitas Riau dan Desa Siabu. Penelitian dilakukan pada bulan Desember 2016 hingga Mei 2017. Lokasi pengambilan sampel terdapat di dua kawasan dengan enam lokasi yang berbeda yaitu Universitas Riau (Arboretum, Kebun FMIPA, Rusunawa) dan Desa Siabu (perumahan warga, Bukit Tentado dan hutan sekunder). Metode sampling mengunakan perangkap layar (light trap) sebagai sumber cahaya digunakan lampu mercuri merk Philips 160 watt. Bagian tubuh ngengat yang diamati adalah kepala, torak, abdomen dan sayap, jumlah karakter morfologi yang diamati adalah 24 karakter. Setiap karakter morfologi diberi skor kemudian dianalisis menggunakan program NTSYS ps versi 2.02i. Hasil yang didapat yaitu 61 spesies dengan 414 individu dari 10 famili ngengat.  Keanekaragaman fenetik ngengat di Universitas Riau dan Desa Siabu berturut turut adalah 87% dan 78%. Ngengat dari kedua kawasan menunjukkan perbedaan pada karakter ukuran, namun tidak pada karakter morfologi dan warna. Terdapat adanya kemiripan sebesar 66% antara ngengat yang dikoleksi dari Universitas Riau (kebun FMIPA, rusunawa) dengan Desa Siabu (Bukit Tentado dan hutan sekunder).Abstract Research on moths is still very few in Indonesia. Especially in the area of Riau University and Siabu Kampar Village. There is no datum about moths. The objective of this research is to analyze the genetic diversity of moths in Riau University and Siabu Vilage, Riau Province based on morphological characters. The study was conducted from December 2016 to May 2017. The moths were collected from two areas with six different locations such as Riau University (arboretum of mathematics and science faculty) and Siabu Village (village residence, Tentado Hill, and secondary forest). The sampling method that used was the light trap, with 160 watts of mercury, was used as a light source. The parts of the body observed were head, thorax, abdomen, and wings with the number of morphological characters observed by 24 characters. Morphological characters were observed and scored and then analyzed using the NTSYSp c version 2.02i software. The results showed that there were 61 species with 414 individuals from 10 families of moths. The genetic diversity of moth in the Riau University and Siabu Village was 87% and 78%. Moths on both locations indicated no difference in the shape and color, but they were different in size. Moths from FMIPA garden and rusunawa had 66% genetic similarity to one from Tentado Hill and secondary forest.

scholarly journals Keragaman Genetik Klon Kakao Lokal Sulawesi Tenggara Berdasarkan Marka SSR dan Karakter Morfologi

2018 ◽  
Vol 5 (3) ◽  
pp. 95
Author(s):  
Nur Kholilatul Izzah ◽  
Budi Martono ◽  
Baharuddin Baharuddin ◽  
Edi Wardiana
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Genetic Similarity ◽  
Genetic Relationships ◽  
Development Program ◽  
Morphological Characters ◽  
Morphological Characterization ◽  
Biology Laboratory ◽  
High Diversity

<em>Molecular and morphological characterization of cacao clones obtained from exploration in Southeast Sulawesi is very important to know their superiority and genetic relationships. Analysis of genetic diversity using molecular markers is also useful for detecting duplication found among collected clones. The research aimed to determine the genetic diversity of local cacao clones derived from Southeast Sulawesi based on SSR markers and morphological characters. The research was conducted at Cacao Research Sub-Station, Lebojaya Village, Konda Subdistrict, South Konawe Regency, Southeast Sulawesi, and Integrated Laboratory of Indonesian Industrial and Beverage Crops Research Institute Sukabumi, and Molecular Biology Laboratory of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development Bogor, from April to November 2015. Genetic diversity analysis was performed on 21 cacao clones covering 19 local clones and 2 national varieties using 22 SSR markers. The molecular characterization results showed that 11 markers are polymorphic, and subsequently used to group cacao clones using NTSYS program. The grouping results divided the cacao clones into 4 main groups at 0.46 genetic similarity values. Based on genetic distance values &gt;0.7, 8 combinations of cacao clones can be selected as parental clones with the expectation to increase the effect of heterosis on progeny. On the other hand, result of morphological characterization generally indicated the diversity between the four cacao groups. Based on molecular and morphological characterization, it can be seen that cacao clones derived from Southeast Sulawesi have a high diversity and can be utilized in the development program of new improved varieties.</em>

scholarly journals Inventory and Characterization of Local Taro Genetic Resources in North Toraja

2018 ◽  
Vol 24 (2) ◽  
pp. 115
Author(s):  
Erina Septianti ◽  
Sahardi Sahardi
Keyword(s):  
Genetic Diversity ◽  
Genetic Resources ◽  
Growth Performance ◽  
Sampling Method ◽  
Morphological Characters ◽  
Optimum Growth ◽  
High Genetic Diversity ◽  
Purposive Sampling

<p>Taro (Colocasia esculenta (L.) Schott) has been cultivated and used as an alternative food in Toraja. Local taro of North Toraja has high genetic diversity, nevertheless, they have not been well characterized. The objective of this study was to inventorying and characterizing the morphological characters of local taro from North Toraja, that could be a key component for registered and maintained as a wealth of local genetic resources. The study was conducted in North Toraja District which includes highland areas in Parodo Baruppu Village, Baruppu District and lowland areas in Mantirotiku Village, Rantepao District. Each region was selected based on initial surveys which showed that these areas were taro cultivation areas. The study was conducted from April to June 2016. Samples were selected based on purposive sampling method and were descriptively observed in-situ in the field on their optimum growth performance. Five local taro varieties were successfully inventoried from survey activities, namely Upe Ungu, Upe Kuning, Upe Putih, Monggo, and Bite. Those taros have similar characters on the shape and leave color but varied on the size and flesh fibre color of the corm. Local taro from North Toraja is widely used as snacks or vegetables as rice substitution. The advantage of North Toraja local taro is easy to cultivate and also has a savory taste, soft, good fragrant aroma, the corm skin after being boiled is very easy to peel, and the texture of the corm is smooth. The price of this local taro is quite expensive because of still difficult to find and have not been widely cultivated.</p>

scholarly journals Evaluation of Potato Cultivars Grown in Saudi Arabia by Morphological Characters and RAPD Markers

2007 ◽  
Vol 12 ◽  
pp. 35
Author(s):  
Abd-Rahman Mohamed M. Al-Moshileh ◽  
Mohamed Zaky El-Shinawy ◽  
Mohamed Ibrahim Motawei
Keyword(s):  
Genetic Diversity ◽  
Genetic Similarity ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Dry Weight ◽  
Morphological Characters ◽  
Potato Cultivars ◽  
The Other ◽  
Polymerase Chain ◽  
Pair Wise Comparison

Genetic diversity of 10 potato cultivars was investigated at the DNA level with the random amplified polymorphic DNA (RAPD) procedure and at the phenotypic level using morphological characters. The results indicated that there were considerable variations among the different studied cultivars. Cultivars Victoria, Frizia and Safaren had the highest chlorophyll content compared to the other cultivars in both seasons. The largest leaf area and leaf dry weight were measured in cultivar Safaren and Mondial in both seasons. Cultivars Aboulx and Mondia produced the highest tuber yield in both seasons. On the other hand, cultivars Victoria and Edward were characterized by their considerably lower yield than other studied cultivars. Specific gravity varied considerably among the different potato cultivars. Thirteen random decamer primers were used to amplify DNA via the polymerase chain reaction and 75 RAPDs were generated. The RAPD profiles obtained were successfully used to differentiate potato cultivars. Based on the pair-wise comparison of amplification products, genetic similarity was estimated. The genetic similarity among all potato cultivars ranged from 50 to 92 %. Cultivars Victoria and Etfadoal presented the least similarity (0.50) while cultivars Mondial and Citrix had the greatest similarity (0.92). Etfadoal cultivar displayed the greatest genetic diversity of all cultivars. A dendrogram was constructed using UPGMA analysis. On the basis of this analysis, the cultivars were grouped into three clusters. The polymorphism detected suggests that RAPD markers are reliable for identification of potato cultivars and could be exploited in genetic mapping of populations to tag economically important traits. 

Study of genetic diversity in wild raspberry (Rubus idaeus L.) germplasm collection using morphological characters and RAPD markers

Biologija ◽  
2008 ◽  
Vol 54 (2) ◽  
pp. 66-74 ◽  
Author(s):  
Jolanta Patamsytė ◽  
Donatas Žvingila ◽  
Juozas Labokas ◽  
Virgilijus Baliuckas ◽  
Laimutė Balčiūnienė ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Germplasm Collection ◽  
Morphological Characters ◽  
Rubus Idaeus

Three new species, two new genera, and new family Biphragmosagittidae (Сhaetognatha) from Southwest Pacific Ocean

2011 ◽  
Vol 20 (1) ◽  
pp. 161-173
Author(s):  
A.P. Kassatkina
Keyword(s):  
New Species ◽  
Type Species ◽  
Morphological Characters ◽  
The Body ◽  
New Order ◽  
New Genera ◽  
New Family ◽  
The Family ◽  
Three New Species

Resuming published and own data, a revision of classification of Chaetognatha is presented. The family Sagittidae Claus & Grobben, 1905 is given a rank of subclass, Sagittiones, characterised, in particular, by the presence of two pairs of sac-like gelatinous structures or two pairs of fins. Besides the order Aphragmophora Tokioka, 1965, it contains the new order Biphragmosagittiformes ord. nov., which is a unique group of Chaetognatha with an unusual combination of morphological characters: the transverse muscles present in both the trunk and the tail sections of the body; the seminal vesicles simple, without internal complex compartments; the presence of two pairs of lateral fins. The only family assigned to the new order, Biphragmosagittidae fam. nov., contains two genera. Diagnoses of the two new genera, Biphragmosagitta gen. nov. (type species B. tarasovi sp. nov. and B. angusticephala sp. nov.) and Biphragmofastigata gen. nov. (type species B. fastigata sp. nov.), detailed descriptions and pictures of the three new species are presented.

scholarly journals Genetic Similarity of Avena sativa L. Varieties as an Example of a Narrow Genetic Pool of Contemporary Cereal Species

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1424
Author(s):  
Magdalena Cieplak ◽  
Sylwia Okoń ◽  
Krystyna Werwińska
Keyword(s):  
Genetic Diversity ◽  
Avena Sativa ◽  
Genetic Similarity ◽  
Breeding Programs ◽  
Central European ◽  
Cereal Species ◽  
Genetic Pool ◽  
Selection Of ◽  
High Genetic Similarity

The assessment of the genetic diversity of cultivated varieties is a very important element of breeding programs. This allows the determination of the level of genetic differentiation of cultivated varieties, their genetic distinctiveness, and is also of great importance in the selection of parental components for crossbreeding. The aim of the present study was to determine the level of genetic diversity of oat varieties currently grown in Central Europe based on two marker systems: ISSR and SCoT. The research conducted showed that both these types of markers were suitable for conducting analyses relating to the assessment of genetic diversity. The calculated coefficients showed that the analyzed cultivars were characterized by a high genetic similarity. However, the UPGMA and PCoA analyses clearly indicated the distinctiveness of the breeding programs conducted in Central European countries. The high genetic similarity of the analyzed forms allow us to conclude that it is necessary to expand the genetic pool of oat varieties. Numerous studies show that landraces may be the donor of genetic variation.

scholarly journals Genetic diversity of Avena strigosa Schreb. ecotypes on the basis of isoenzyme markers

2009 ◽  
Vol 15 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Katarzyna Kubiak
Keyword(s):  
Genetic Diversity ◽  
Genetic Similarity ◽  
Avena Strigosa ◽  
Total Genetic Diversity ◽  
Geographical Regions ◽  
The World ◽  
Total Variability ◽  
The Mean ◽  
Intrapopulation Diversity ◽  
Very High

Genetic diversity ofAvena strigosaSchreb. ecotypes on the basis of isoenzyme markersGenetic diversity was analyzed in 19 ecotypes of the diploid oatA. strigosaoriginating from various geographical regions of the world. Six isoenzyme systems (AAT, ACP, EST, LAP, MDH, PX) were studied and 16 loci were identified. Only two loci (Est4andMdh2) were polymorphic. Ecotypes were characterized by the percentage of polymorphic loci (P=3.3%), the mean number of alleles per locus (A=1.04) and intrapopulation diversity (HS=0.013). Total genetic diversity (HT=0.07) and interpopulation diversity (DST=0.057) were examined as well. The value of the coefficient of gene differentiation (GST=0.821) indicated that diversity among populations was an important contributor to total variability. Genetic similarity betweenA. strigosapopulations was very high (IN=0.94). Cluster analysis did not demonstrate strongly differentiated groups among the ecotypes examined.

Steinernema borjomiense n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Georgia

Nematology ◽  
2018 ◽  
Vol 20 (7) ◽  
pp. 653-669 ◽  
Author(s):  
Oleg Gorgadze ◽  
Elena Fanelli ◽  
Manana Lortkhipanidze ◽  
Alberto Troccoli ◽  
Medea Burjanadze ◽  
...  
Keyword(s):  
New Species ◽  
18S Rrna ◽  
Entomopathogenic Nematode ◽  
Phylogenetic Analyses ◽  
Tail Length ◽  
Morphological Characters ◽  
18S Rrna Gene ◽  
The Body ◽  
Rrna Gene ◽  
A New Species

Summary A new species of entomopathogenic nematode, Steinernema borjomiense n. sp., was isolated from the body of the host insect, Oryctes nasicornis (Coleoptera: Scarabaeidae), in Georgia, in the territory of Borjomi-Kharagauli. Morphological characters indicate that the new species is closely related to species of the feltiae-group. The infective juveniles are characterised by the following morphological characters: body length of 879 (777-989) μm, distance between the head and excretory pore = 72 (62-80) μm, pharynx length = 132 (122-142) μm, tail length = 70 (60-80) μm, ratio a = 26.3 (23.0-29.3), H% = 45 (40-51), D% = 54 (47-59), E% = 102 (95-115), and lateral fields consisting of seven ridges (eight incisures) at mid-body. Steinernema borjomiense n. sp. was molecularly characterised by sequencing three ribosomal regions (the ITS, the D2-D3 expansion domains and the 18S rRNA gene) and the mitochondrial COI gene. Phylogenetic analyses revealed that S. borjomiense n. sp. differs from all other known species of Steinernema and is a member of the monticolum-group.

ANALISA LOGAM BESI (Fe) DI SUNGAI PASAR DAERAH BELANGWETAN KLATEN DENGAN METODE SPEKTROFOTOMETRI SERAPAN ATOM

2017 ◽  
Vol 2 (1) ◽  
pp. 39
Author(s):  
Rahmi Nurhaini ◽  
Arief Affandi
Keyword(s):  
Heavy Metals ◽  
Sampling Method ◽  
Research Result ◽  
The Body ◽  
Clean Water ◽  
Observational Method ◽  
The Mean ◽  
The Republic

Iron (Fe) is one of many heavy metals that is corrosive resistant, dense, and has a low melting point. If accumulated in the body, the metal can cause some medical conditions, such as irritation to skin and eyes, breathing problems, and in the long term, cancer. This research aims to know generally the spread of metallic iron (Fe) in the river Pasar in Belangwetan, Klaten. This study was conducted using an observational method in which researchers did not examine the effects of interventions. Sampling was done using purposive sampling method taken from three points, namely the upper, middle, lower. Determination of iron levels by Atomic Absorption Spectrophotometer (AAS) obtained a positive result, and the data was processed using SPSS to determine the Mean and Standard Deviation. Of the research result, it could be known the Mean score was 2.33 ppm and SD was 0.0352. The result of this research indicated that the levels of iron in the river Pasar in Belangwetan were 2.33 ppm. It means that the levels violate the regulation of the Minister of Health of the Republic of Indonesia, which is not more than 1mg/L (1ppm) in the clean water

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