ISOLATION AND CHARACTERIZATION OF L. parafarraginis (KU495926) INHIBITING MULTIDRUG-RESISTANT AND EXTENDED SPECTRUM ?ETA-LACTAMASE GRAM-NEGATIVE BACTERIA

Multidrug-resistant Gram-negative bacteria, especially for extended-spectrum β-lactamases-producing and carbapenemase-producing Enterobacterales , are disseminating rapidly around the world. Treatment options for these infections are limited, which prompt the development of novel or combinational therapies to combat the infections caused by multidrug-resistant pathogens.

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Extended Spectrum β-Lactamase (ESBL) Producing Multidrug Resistant Gram-Negative Bacteria from Various Clinical Specimens of Patients Visiting a Tertiary Care Hospital

Tribhuvan University Journal of Microbiology ◽

10.3126/tujm.v4i0.21667 ◽

2018 ◽

Vol 4 ◽

pp. 1-8

Author(s):

Albert Ghimire ◽

Bipesh Acharya ◽

Reshma Tuladhar

Keyword(s):

Susceptibility Testing ◽

Multidrug Resistant ◽

Hospitalized Patients ◽

Diffusion Method ◽

Gram Negative Bacteria ◽

Care Hospital ◽

Gram Negative ◽

Clinical Specimens ◽

Extended Spectrum ◽

Esbl Producers

Objectives: The purpose of this study was to assess multidrug resistance and Extended Spectrum β-Lactamase (ESBL) production in Gram negative bacterial pathogens.Methods: The study included clinical specimens sent for routine culture and antibiotic susceptibility testing. A total of 469 different clinical specimens were processed according to the standard methodology. The isolates were identified by standard microbiological procedures and subjected to antimicrobial susceptibility testing by modified Kirby-Bauer disk diffusion method. Production of ESBL was determined by combined disk method.Results: Of the total sample processed, 80 (17.0%) Gram negative bacteria were isolated and 82.5% of them were multidrug resistant (MDR). From the total MDR isolates, 47% were ESBL positive. The higher rate of growth among Intensive Care Units (ICUs) patients was found statistically significant. Higher prevalence of MDR isolates was observed in blood and pus specimens. The majority of the ESBL producers were Escherichia coli (38.7%). Higher rate of ESBL producers was detected from blood (55.6%). Polymyxin B, imipenem and amikacin were the most effective antibiotics against Acinetobacter spp. and Pseudomonas aeruginosa whereas imipenem, amikacin, meropenem were the most effective antibiotics against Enterobacteriaceae.Conclusion: Higher prevalence of ESBL producing MDR Gram negative pathogens in hospitalized patients indicates these bacteria are important health care associated pathogens and requires proper infection control measures that check the transfer of MDR and β-lactamase producing bacterial pathogens among the hospitalized patients.

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Isolation and characterization of 3-acetamido-3,6-dideoxy-L-glucose from the core oligosaccharides obtained from the aquatic gram-negative bacteria Aeromonas hydrophila and Vibrio anguillarum

Canadian Journal of Biochemistry ◽

10.1139/o81-122 ◽

1981 ◽

Vol 59 (11-12) ◽

pp. 877-879 ◽

Cited By ~ 4

Author(s):

Joseph H. Banoub ◽

Derek H. Shaw

Keyword(s):

Aeromonas Hydrophila ◽

Vibrio Anguillarum ◽

Amino Sugar ◽

Gram Negative Bacteria ◽

Core Oligosaccharide ◽

Gram Negative ◽

The Core ◽

Isolation And Characterization ◽

Bacterial Lipopolysaccharides

The amino sugar 3-acetamido-3,6-dideoxy-L-glucose has been isolated and characterized from the core oligosaccharide obtained from the bacterial lipopolysaccharides of Aeromonas hydrophila and Vibrio anguillarum. This is the first occasion in which a dideoxyamino sugar has been confirmed as a constituent of the core oligosaccharide rather than the O-polysaccharide.

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Epidemiology and molecular characterization of multidrug-resistant Gram-negative bacteria in Southeast Asia

Antimicrobial Resistance and Infection Control ◽

10.1186/s13756-016-0115-6 ◽

2016 ◽

Vol 5 (1) ◽

Cited By ~ 40

Author(s):

Nuntra Suwantarat ◽

Karen C. Carroll

Keyword(s):

Southeast Asia ◽

Molecular Characterization ◽

Multidrug Resistant ◽

Gram Negative Bacteria ◽

Gram Negative

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Isolation and characterization of insertion sequence elements from gram-negative bacteria by using new broad-host-range, positive selection vectors.

Journal of Bacteriology ◽

10.1128/jb.173.4.1502-1508.1991 ◽

1991 ◽

Vol 173 (4) ◽

pp. 1502-1508 ◽

Cited By ~ 53

Author(s):

R Simon ◽

B Hötte ◽

B Klauke ◽

B Kosier

Keyword(s):

Positive Selection ◽

Host Range ◽

Insertion Sequence ◽

Broad Host Range ◽

Gram Negative Bacteria ◽

Gram Negative ◽

Isolation And Characterization ◽

Sequence Elements ◽

Insertion Sequence Elements

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475. High Rate of Extended-Spectrum β-Lactamase Producing Gram-Negative Infections and Associated Mortality in Ethiopia: A Systematic Review and Meta-Analysis

Open Forum Infectious Diseases ◽

10.1093/ofid/ofz360.548 ◽

2019 ◽

Vol 6 (Supplement_2) ◽

pp. S232-S232 ◽

Cited By ~ 1

Author(s):

Tafese B Tufa ◽

Takele Beyene Tufa ◽

Fuchs André ◽

Feldt Torsten

Keyword(s):

Systematic Review ◽

Meta Analysis ◽

Multidrug Resistant ◽

High Rate ◽

Clinical Samples ◽

Published Data ◽

Gram Negative Bacteria ◽

Salmonella Spp ◽

Gram Negative ◽

Extended Spectrum

Abstract Background Extended-spectrum β-lactamase (ESBL)-producing Gram-negative bacteria have become a serious threat to global health. The rapid increase of ESBL-producing bacteria is associated with high mortality due to ineffective antibiotic treatment. To date, regular surveillance of multidrug-resistant (MDR) pathogens is lacking in Ethiopia. For this report, published data regarding ESBL-producing bacteria in different regions of Ethiopia were reviewed systematically. To our knowledge, this is the first systematic review from Ethiopia on ESBL-producing infections and associated mortality in the country. Methods A literature search was conducted in PubMed, PubMed Central, and Google Scholar from January 1, 1990 to April 28, 2019, using the following search terms: “ESBL producing Enterobacteriaceae,” “Gram-negative bacteria infection associated mortality,” and “Ethiopia.” Patient mortality associated with infections by ESBL-producing Gram-negative bacteria was recorded. Results Fourteen publications qualified for review. Totally, 1,782 Gram-negative bacteria isolated from 5,191 clinical samples were included. The phenotypic pooled rate of ESBL-producing Gram-negatives was estimated to be 52.9(95% CI: 50.5%–55.4%). Among different species, ESBL rates were 65. 7% (262/399) Klebsiella spp., 60.6% (20/33) for Enterobacter spp., 47.8% (22/46) for Citrobacter spp., 47.0% (383/815) for E. coli, 45.7% (85/186) for Salmonella spp., 27.8%(15/54) for Proteus spp., 16.7%(4/24) for P. aeruginosa, 14.3% (3/21) for Acinetobacter spps., and 40.5% (15/37) for others, respectively. ESBL genes were confirmed in three studies. blaCTX-M-1 and blaTEM were the predominately detected genes. Two studies reported mortality associated with Gram-negative infections and 86% (12/14) of the patients infected with ESBL-producing bacteria died. Conclusion In this meta-analysis, the pooled phenotypic prevalence of ESBL-producing pathogens is considerably high. Also, the mortality due to ESBL-producers is high but data are scarce. This highlights the need for establishing and upgrading of clinical microbiology laboratories in the country for routine antibiotic susceptibility testing. The capacity to detect ESBL genes is desirable for continuous surveillance of MDR. Disclosures All authors: No reported disclosures.

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Heavy-metal resistance in Gram-negative bacteria isolated from Kongsfjord, Arctic

Canadian Journal of Microbiology ◽

10.1139/cjm-2014-0803 ◽

2015 ◽

Vol 61 (6) ◽

pp. 429-435 ◽

Cited By ~ 18

Author(s):

C.S. Neethu ◽

K.M. Mujeeb Rahiman ◽

A.V. Saramma ◽

A.A. Mohamed Hatha

Keyword(s):

Heavy Metals ◽

Inhibitory Concentration ◽

Heavy Metal Resistance ◽

Metal Resistance ◽

The Arctic ◽

Gram Negative Bacteria ◽

Gram Negative ◽

Isolation And Characterization ◽

Tube Dilution

Isolation and characterization of heterotrophic Gram-negative bacteria was carried out from the sediment and water samples collected from Kongsfjord, Arctic. In this study, the potential of Arctic bacteria to tolerate heavy metals that are of ecological significance to the Arctic (selenium (Se), mercury (Hg), cadmium (Cd), copper (Cu), lead (Pb), and zinc (Zn)) was investigated. Quantitative assay of 130 isolates by means of plate diffusion and tube dilution methods was carried out by incorporation of different concentrations of metals. Growth in Se and Pb at a concentration of 3000 μg/L was significantly lower (P ≤ 0.0001) than at 2000 μg/L. The minimum inhibitory concentration for Cd and Hg was 50 μg/L (P ≤ 0.0001, F = 264.23 and P ≤ 0.0001, F = 291.08, respectively) even though in the tube dilution test, Hg-containing tubes showed much less growth, revealing its superior toxicity to Cd. Thus, the level of toxicity of heavy metals was found to be in the order of Hg > Cd > Cu > Zn > Pb > Se. Multiple-metal-resistant isolates were investigated for their resistance against antibiotics, and a positive correlation was observed between antibiotic and metal resistance for all the isolates tested. The resistant organisms thus observed might influence the organic and inorganic cycles in the Arctic and affect the ecosystem.

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