scholarly journals Вплив препаратів з антиоксидантними властивостями на стан антиоксидантної системи сперматозоїдів при екскреторнo-токсичній формі неплідності чоловіків

2013 ◽  
Vol 4 (2) ◽  
pp. 52-56 ◽  
Author(s):  
O.K. Onufrovych ◽  
D.Z. Vorobets ◽  
Z.D. Vorobets
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Vitamin E ◽  
Vitamin C ◽  
Glutathione Peroxidase ◽  
Antioxidant System ◽  
Zinc Sulfate ◽  
Antioxidant Properties ◽  
Glutathione Antioxidant System ◽  
Male Sex

Since the development of many disorders of the reproductive function in men involves processes of free radical oxidation, the purpose of this study was to form an evaluation of the pro- and antioxidant status of sperm and to restore its biological usefulness in men with excretory-toxic forms of infertility by using drugs with antioxidant properties. It is shown that excretory-toxic forms of infertility in men are mostly caused by such infectious agents as Chlamydia (22%), Chlamydia + Ureaplasma (16%), Chlamydia + Trichomonas (13%), Ureaplasma (10%). This reduces the total number of sperm in the ejaculate by 2.7 times, and motility by 1.8 times. The number of abnormal forms increases by 1.75 times. With the development of chronic inflammation of the male sex organs sperm lipid peroxidation increases by 1.3 times while the activity of glutathione peroxidase decreases (by 2.3 times) and that of glutathione reductase (by 1.7 times). We observed a close correlation between the low biological quality of sperm (low concentration, low number and motility of sperm in the ejaculate) with activation of lipid peroxidation and inhibition of activity of the glutathione antioxidant system. In the case of superoxide dismutase, the negative impact of reactive oxygen species on this enzyme was not observed. A course of drugs with antioxidant properties – vitamin E, vitamin C and zinc sulfate leads to improvement in the indicators on the spermagram (mostly sperm mobility and morphology), to reduction of the number of peroxide compounds and activation of the glutathione antioxidant system. In this case, the activity of glutathione peroxidase is increased by 1.5 times and the activity of glutathione reductase by 1.3 times. The activity of superoxide dismutase at the same time approaches the norm for zoospermia. The data obtained show that one of the pathogenic factors of the chronic inflammation of male sex organs, considered as a main developmental reason for infertility in its excretory-toxic form, is the increase in activity of the peroxide oxygen lipids of the sperm membrane and decompensation of the enzyme activity of the glutathione antioxidant system. Our data indicate that the use as medicines of vitamin E, vitamin C and zinc sulfate combined with antibiotic therapy would be highly effective in the treatment of male infertility.

scholarly journals Вплив хлориду кадмію на стан антиоксидантної системи у печінці щурів

2013 ◽  
pp. 102-103
Author(s):  
Б. В. Гутий
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Glutathione Peroxidase ◽  
Antioxidant System ◽  
Cadmium Chloride

Розкрито особливості антиоксидантної системиорганізму щурів за хронічного кадмієвого токсикозу.Встановлено, що хлорид кадмію у токсичній дозісприяє зниженню активності ферментної й нефер-ментної системи антиоксидантного захисту, на щовказує зниження ферментів глутатіонпероксидази,глутатіонредуктази, супероксиддисмутази, катала-зи та відновленого глутатіону у печінці щурів. Ре-зультати досліджень вказують на те, що хронічнийкадмієвий токсикоз призводить до посиленої акти-вації процесів ліпопероксидації. The features of the antioxidant system of rats with chronic cadmium toxicosiare disclosed. It wasresearched that cadmium chloride in toxic doses reduces enzyme activity of antioxidant system, asindicated by the decrease in enzyme glutathione peroxidase, hlutationreduktazy, superoxide dismutase,catalase and restored glutathione in the liver and blood of rats. The results indicate that chroniccadmium toxicosis leads to enhanced activation of lipid peroxidation.

scholarly journals INFLUENCE OF «METISEVIT» ON THE ACTIVITY OF ENZYME AND NONENZYME LINK OF ANTIOXIDANT PROTECTION UNDER THE BULL’S BODY CADMIUM LOADING

2016 ◽  
Vol 18 (2(66)) ◽  
pp. 52-59
Author(s):  
B.V. Gutyj ◽  
Y. Lavryshyn ◽  
V. Binkevych ◽  
O. Binkevych ◽  
О. Paladischuk ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Vitamin E ◽  
Glutathione Peroxidase ◽  
Antioxidant Defense ◽  
Cadmium Stress ◽  
Antioxidant Defense System ◽  
The Body ◽  
Antioxidant Protection ◽  
Young Cattle

The article contains the research results of the effect of cadmium chloride on the indexes of enzyme and nonenzyme systems of  antioxidant defense system in young cattle, such as the activity of catalase, superoxide dismutase, glutathione peroxidase, glutathione levels of vitamins A and E. It is established that feeding calves at a dose of toxicant 0.04 mg / kg activity of catalase, superoxide dismutase, glutathione peroxidase, glutathione levels of vitamins A and E in the blood of experimental animals decreased throughout the experiment. The lowest indicators of antioxidant in the blood of young cattle is set on the twenty -fourth day of the experiment, which is associated with increased activation of lipid peroxidation and the balance between antioxidant system and lipid peroxidation intensity. Given the cadmium load of young cattle it is used a new integrated drug with antioxidant action «Metisevit», which includes metifen, sodium selenite and vitamin E wich is founded as stimulating effects on the activity of antioxidant protection. In particular,it is established probable increase in activity of catalase, superoxide dismutase, glutathione peroxidase, glutathione levels, vitamin A and vitamin E in the blood of young cattle, which has performed cadmium stress. These changes occur through comprehensive action components of the drug «Metisevit» that leads to the normalization of metabolic processes and free radical in the body of the bull. The results of the research indicate antioxidant drug «Metisevit» in the application of its young cattle and the validity of his administration to improve the body's antioxidant status of chronic cadmium toxicosis.

Estrogen administration, postexercise tissue oxidative stress and vitamin C status in male rats

1998 ◽  
Vol 76 (10-11) ◽  
pp. 952-960 ◽  
Author(s):  
Peter M Tiidus ◽  
Eric Bombardier ◽  
Nick Hidiroglou ◽  
Rene Madere
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Vitamin E ◽  
Vitamin C ◽  
Glutathione Peroxidase ◽  
Antioxidant Status ◽  
Male Rats ◽  
Glutathione Status ◽  
Estrogen Administration ◽  
Tissue Vitamin

Estrogen can putatively act as an antioxidant and protect tissues from exercise-induced oxidative stress. To test the in vivo efficacy of estrogen, the effects of 2 weeks of daily estrogen (40 µg·kg-1 body weight beta-estradiol 3-benzoate) injection on indices of immediate postexercise oxidative stress and antioxidant status were determined in adult male rats, with and without 8 weeks of prior dietary vitamin E deprivation. The treadmill running protocol (60 min at 21 m·min-1, 12% grade) induced significant oxidative stress as indicated by muscle glutathione status. Estrogen administration had little effect on postexercise tissue glutathione status, superoxide dismutase and glutathione peroxidase activity, and vitamin E levels. Estrogen administration induced significant reductions in muscle, liver, and heart vitamin C concentrations following exercise, as well as in unexercised male rats. Tissue vitamin C loss was not directly mediated through liver glycogen or glutathione status. Thus, estrogen administration generally did not appear to influence postexercise tissue indices of oxidative stress or antioxidant status and may have contributed to a decline in overall antioxidant protection by inducing losses in tissue vitamin C content.Key words: glutathione, vitamin E, muscle, superoxide dismutase, glutathione peroxidase.

scholarly journals Protective Effect of Dorema glabrum on Induced Oxidative Stress by Diazinon in Hippocampus of Rat

2019 ◽  
pp. 1-7
Author(s):  
Mina Adampourezare ◽  
Parisa Sistani ◽  
Homeira Hatami Nemati
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Catalase Activity ◽  
Antioxidant Properties ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Male Wistar Rats ◽  
Pre Treatment

Introduction: Diazinon (DZN) administration produces lipid peroxidation as an indicator of oxidative stress in the brain. Some medicinal plants such as Dorema glabrum has antioxidant properties, so can be used as an antioxidant that may protect neurons from oxidative stress. The aim of present study was to investigate the effect of D. glabrum against DZN-induced oxidative stress in hippocampus. Methods: Twenty-four adult male Wistar rats were used in this study. The rats randomly were divided into four groups including a control group, and two groups received different doses of D. glabrum (40 and 80 mg/kg) as pre-treatment for 21 days with DZN (100 mg/Kg) that was injected intraperitoneally (ip) in last day of D. glabrum usage, and one group received only DZN. Thiobarbituric acid reactive substances (TBARS), which are the indicators of lipid peroxidation, and the activities of antioxidant enzymes (glutathione peroxidase, superoxide dismutase and catalase) were determined in the ratsʼ hippocampus. Results: Administration of DZN significantly increased TBARS levels and superoxide dismutase activity and decreased glutathione peroxidase activity but there were no significant changes in catalase activity in the hippocampus. Combined D. glabrum and DZN treatment, caused a significant increase in glutathione peroxidase, a significant decrease of TBARS and a significant decrease in superoxide dismutase and again no significant changes in catalase activity in the rats’ hippocampus when compared to the rats treated with DZN. Conclusion: Our study demonstrated that D. glabrum had an amelioratory effect on oxidative stress induced by DZN.

The Effect of Hemodialysis and Acetate-Free Biofiltration on Anemia

2000 ◽  
Vol 23 (3) ◽  
pp. 173-180 ◽  
Author(s):  
J. Eiselt ◽  
J. Racek ◽  
K. Opatrnyjr
Keyword(s):  
Lipid Peroxidation ◽  
Renal Failure ◽  
Superoxide Dismutase ◽  
Free Radical ◽  
Chronic Renal Failure ◽  
Glutathione Peroxidase ◽  
Antioxidant Capacity ◽  
Antioxidant System ◽  
Transferrin Saturation ◽  
Significant Difference

The authors monitored, for a period of 12 months, anemia-, nutrition-, and free radical-related parameters and the rHuEPO dose required to maintain target hemoglobin (Hb) in 20 patients with chronic renal failure. Ten patients each were randomized for treatment by either acetate-free biofiltration (AFB) or low-flux hemodialysis (HD). At baseline, Hb levels were 102±2 (AFB) vs. 98±2 g/L (HD) (not significant difference, NS), the rHuEPO dose was 4050±976 vs. 5100±1538 IU/week (NS). Compared with baseline and with HD, lower rHuEPO doses were required during AFB at months 8, 9, 10 and 11, and 12 when they were 2100±510 (AFB) vs. 6000±1153 (HD), p=0.008. Prealbumin, transferrin and cholinesterase levels rose in the AFB group. Kt/V, albumin, transferrin saturation, aluminium, bicarbonate in serum, superoxide dismutase and glutathione peroxidase in erythrocytes, and malondialdehyde and antioxidant capacity in plasma did not differ between the AFB and HD groups. In terms of anemia control, AFB using an AN69 membrane was found to be more advantageous than low-flux HD, AFB improves some nutritional parameters. The compared methods do not differ in their effect on lipid peroxidation and the antioxidant system. (Int J Artif Organs 2000; 23: 173–80)

scholarly journals Effects of Pre-Exposure To Waterborne Selenium On Redox Homeostasis and Humoral Innate Immune Parameters in African Catfish, Clarias Gariepinus (Burchell, 1822) Experimentally Challenged With Serratia Marcescens

2021 ◽  
Author(s):  
Joseph Adeyemi ◽  
Germaine A. Ogunwole ◽  
Olufemi S. Bamidele ◽  
Chris O. Adedire
Keyword(s):  
Nitric Oxide ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Respiratory Burst ◽  
Reduced Glutathione ◽  
Redox Homeostasis ◽  
Clarias Gariepinus ◽  
Antioxidant Properties

Abstract Bacterial infections have been associated with immune dysfunction and oxidative stress in cultured fish species while essential elements could boost immunity and exhibit antioxidant properties in fish. This study was therefore aimed at determining the effects of pre-exposure to waterborne selenium on humoral immunity and redox status of Clarias gariepinus experimentally challenged with Serratia marcescens. Juveniles C. gariepinus were pre-exposed to 50 µg/L selenium for 14 days after which they were challenged with 5 × 103 CFU/mL of S. marcescens via oral gavage for 24 or 48 h. The control fish were not exposed to selenium and not challenged with bacteria. Thereafter, fish were sacrificed, blood collected into EDTA bottles for the determination of plasma nitric oxide levels and respiratory burst, and the liver excised for the determination of reduced glutathione, lipid peroxidation, and activities of catalase, superoxide dismutase, and glutathione peroxidase. Fish that were challenged with bacteria without pre-exposure to selenium had elevated levels of nitric oxide, reduced glutathione, lipid peroxidation, and a higher activity of glutathione peroxidase and respiratory burst but a significant decrease in the activity of superoxide dismutase compared to the fish that were pre-exposed to selenium prior to bacterial challenge at both 24 and 48 h post-infection periods. The results from this study showed that infection with S. marcescens is capable of disrupting the immune system and redox homeostasis in C. gariepinus, while pre-exposure to selenium has the ability to improve the physiological status of fish that were challenged with bacteria probably through its antioxidant properties.

scholarly journals Influence of cadmium loading on the state of the antioxidant system in the organism of bulls

2016 ◽  
Vol 24 (1) ◽  
pp. 96-102 ◽  
Author(s):  
B. V. Gutyj ◽  
S. D. Mursjka ◽  
D. F. Hufrij ◽  
I. I. Hariv ◽  
N. D. Levkivska ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Antioxidant System ◽  
Cadmium Chloride ◽  
Antioxidant Defense ◽  
Reduced Glutathione ◽  
Antioxidant Defense System ◽  
Defense System ◽  
Young Cattle

This article presents the results of research on the influence of cadmium loading on the state level of enzymatic and non-enzymatic antioxidant links of the antioxidant defense system of the organisms of young cattle, such as the activity of catalase, superoxide dismutase, glutathione peroxidase, glutathione levels, selenium, vitamins A and E. It was found that feeding bull calves with cadmium chloride at doses of 0.03 and 0.05 mg/kg of body weight helped to reduce both the enzymatic and non-enzymatic link of antioxidant protection (superoxide dismutase 31%, catalase 13%, glutathione peroxidase 23%, reduced glutathione 10%, vitamin A 28%, vitamin E 31%, selenium 20%). Toxic effects of cadmium promotes change in steady-state concentrations of radical metabolites О2–,ОН˙, НО2˙, which, in turn, trigger the process of lipid peroxidation. The lowest level of indicators of antioxidant defense system in the blood of young cattle was registered on the sixteenth and twenty-fourth days of the experiment, which is associated with increased activation of lipid peroxidation and the disturbaance of the balance between the antioxidant system and lipid peroxidation intensity. The activity of the antioxidant defense system in the blood was different for calves fed with cadmium chloride at doses of 0.03 and 0.05 mg/kg of animal mass. The more cadmium chloride in the feed, the lower the activity of the antioxidant defense system of the calves’ organisms was registered. Thus cadmium chloride depresses the antioxidant defense system, which specifically involves lowering the activity of enzymatic links (catalase, superoxide dismutase, glutathione peroxidase) and non-enzymatic links (reduced glutathione, selenium, vitamins A and E).

scholarly journals CONDITION OF LIPID PEROXIDE OXIDATION AND ANTIOXIDANT SYSTEM IN PATIENTS WITH INFECTIOUS MONONUCLEOSIS

2018 ◽  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Glutathione Peroxidase ◽  
Blood Plasma ◽  
Glutathione Reductase ◽  
Antioxidant System ◽  
Infectious Mononucleosis ◽  
Malonic Dialdehyde

The indicators of the activity of lipid peroxidation and the antioxidant system were studied in dynamics in 158 patients with infectious mononucleosis depending on the severity of the clinical course of the disease. It is proved that lipid peroxidation is significantly activated in patients with infectious mononucleosis as the severity of the disease increases and therefore increases the oxidative activity of blood plasma, the concentration of dyne conjugates and malondialdehyde in the blood, decreases the activity of antioxidant enzymes of erythrocytes (catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase), glutathione peroxidase and glutathione reductase activity in plasma and lowering the concentration of total and reduced glutathione in the blood, as well as reduce the antioxidant activity of blood plasma and erythrocytes. Lipid peroxidation significantly increase and the activity of the antioxidant system decrease in patients with infectious mononucleosis in the acute period of the disease, as evidenced by the increased concentration of dyne conjugates, malonic dialdehyde and total oxidative plasma activity in the blood, reduced activity of the total antioxidant activity of plasma and erythrocytes, the decrease in the activity of antioxidant enzymes of erythrocytes catalase, superoxide dismutase, glutathione peroxidase, glutathione peroxidase and glutathione reductase of blood plasma, the decrease in the concentration of glutathione in the blood. The most pronounced disorders of lipid peroxidation and antioxidant system activity were found in patients with moderate-severe and severe infectious mononucleosis. The development of cytolytic syndrome in infectious mononucleosis is associated with the action of reactive oxygen forms species and lipid hydroperoxide.

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