scholarly journals Effect of CuSO4 on the growth of Brassica juncea L.

2020 ◽  
Vol 10 (5) ◽  
pp. 245-247
Author(s):  
M.R. Pratik ◽  
B. Bavi ◽  
B. Bavi
Keyword(s):  
Seed Germination ◽  
Brassica Juncea ◽  
Copper Sulfate ◽  
Copper Sulphate ◽  
Germination Rate ◽  
High Concentration ◽  
Black Mustard ◽  
Germination And Growth

Seeds of Black Mustard (Brassica juncea) were sowed in a paper cup with copper sulfate at different concentration. This experiment aimed to determine the effect of the variation in concentration of copper sulphate on the germination and growth of seeds of Brassica juncea. We wanted to establish which is the highest concentration of copper that the seeds of Black Mustard can tolerate. Seedlings growth investigation and measurements were made after 5 days. The seed germination rate was low to no for the high concentration and for control that increased dramatically with the decrease in concentration. At high concentration, no growth was seen.

scholarly journals The Influence Of Zinc On Seed Germination And Growth In The First Ontogenetic Stages In The Species Cucumis Melo L.

2015 ◽  
Vol 9 (2) ◽  
pp. 215-228 ◽  
Author(s):  
Anişoara Stratu ◽  
Naela Costică
Keyword(s):  
Seed Germination ◽  
Cucumis Melo ◽  
Growth Process ◽  
Lateral Roots ◽  
Tolerance Index ◽  
High Concentration ◽  
Seedling Vigour ◽  
Ontogenetic Stages ◽  
Vigour Index ◽  
Germination And Growth

AbstractThe paper presents the results of a study regarding the influence of treatment with zinc in different concentrations (50 mg∕l, 100 mg∕l, 200 mg ∕l, 300mg/l, 400 mg ∕l, 500 mg/l, 600 mg ∕l) on seed germination and growth in early ontogenetic stages ofCucumis meloL. We analyzed the following indicators: the percentage of germinated seeds; the length of root, the length of the hypocotyl and the length of the seedling; the number of the laterale roots; the tolerance index and the seedling vigor index. The results underline the specific variations of analysed indicators, depending on the concentrations used for the treatments of seeds. The concentrations used for treatment do not influence negatively the seed germination, but affected the seedling growth (especially the root elongation), the formation and growth process of lateral roots and the seedling vigour index. The delay effect of growth process is very pronounced in the case of high concentration.

scholarly journals Inducing salt tolerance in sweet corn by magnetic priming

2017 ◽  
Vol 109 (1) ◽  
pp. 89 ◽  
Author(s):  
Soheil Karimi ◽  
Saeid ESHGHI ◽  
Saeid KARIMI ◽  
Saman HASAN-NEZHADIAN
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Plant Growth ◽  
Sweet Corn ◽  
Germination Rate ◽  
Nacl Stress ◽  
Proline Accumulation ◽  
Membrane Thermostability ◽  
Germination And Growth ◽  
Magnetic Priming

<p>This study evaluates seed germination and growth of sweet corn under NaCl stress (0, 50, and 100 mM), after exposing the seeds to weak (15 mT) or strong (150 mT) magnetic fields (MF) for different durations (0, 6, 12, and 24 hours). Salinity reduced seed germination and plant growth. MF treatments enhanced rate and percentage of germination and improved plant growth, regardless of salinity. Higher germination rate was obtained by the stronger MF, however, the seedling were more vigorous after priming with 15 mT MF. Proline accumulation was observed in parallel with the loss of plant water content under 100 mM NaCl stress. MF prevented proline accumulation by improving water absorption. Positive correlation between H<sub>2</sub>O<sub>2</sub> accumulation and membrane thermostability (MTI) was found after MF treatments, which revealed that MF primed the plant for salinity by H<sub>2</sub>O<sub>2</sub> signaling. However, over-accumulation of H<sub>2</sub>O<sub>2</sub> after prolonged MF exposure adversely affected MTI under severe salt stress. In conclusion, magnetic priming for 6 hours was suggested for enhancing germination and growth of sweet corn under salt stress.</p>

scholarly journals Symbiotic and Asymbiotic Germination of Dendrobium officinale (Orchidaceae) Respond Differently to Exogenous Gibberellins

2020 ◽  
Vol 21 (17) ◽  
pp. 6104 ◽  
Author(s):  
Juan Chen ◽  
Bo Yan ◽  
Yanjing Tang ◽  
Yongmei Xing ◽  
Yang Li ◽  
...  
Keyword(s):  
Seed Germination ◽  
Mycorrhizal Fungi ◽  
Calcium Binding ◽  
Germination Rate ◽  
Dendrobium Officinale ◽  
Fungal Colonization ◽  
Fungal Mycelium ◽  
High Concentration ◽  
Symbiotic Germination ◽  
Asymbiotic Germination

Seeds of almost all orchids depend on mycorrhizal fungi to induce their germination in the wild. The regulation of this symbiotic germination of orchid seeds involves complex crosstalk interactions between mycorrhizal establishment and the germination process. The aim of this study was to investigate the effect of gibberellins (GAs) on the symbiotic germination of Dendrobium officinale seeds and its functioning in the mutualistic interaction between orchid species and their mycobionts. To do this, we used liquid chromatograph-mass spectrometer to quantify endogenous hormones across different development stages between symbiotic and asymbiotic germination of D. officinale, as well as real-time quantitative PCR to investigate gene expression levels during seed germination under the different treatment concentrations of exogenous gibberellic acids (GA3). Our results showed that the level of endogenous GA3 was not significantly different between the asymbiotic and symbiotic germination groups, but the ratio of GA3 and abscisic acids (ABA) was significantly higher during symbiotic germination than asymbiotic germination. Exogenous GA3 treatment showed that a high concentration of GA3 could inhibit fungal colonization in the embryo cell and decrease the seed germination rate, but did not significantly affect asymbiotic germination or the growth of the free-living fungal mycelium. The expression of genes involved in the common symbiotic pathway (e.g., calcium-binding protein and calcium-dependent protein kinase) responded to the changed concentrations of exogenous GA3. Taken together, our results demonstrate that GA3 is probably a key signal molecule for crosstalk between the seed germination pathway and mycorrhiza symbiosis during the orchid seed symbiotic germination.

scholarly journals The Effects of Temperature, Light and Moisture on the Seed Germination of Siphonostegia chinensis Benth.

2019 ◽  
Vol 1 (2) ◽  
Author(s):  
Yongji Wang
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Germination Index ◽  
High Concentration ◽  
Complete Darkness ◽  
Germination Potential ◽  
Medium Level ◽  
Different Temperatures ◽  
High Level ◽  
Germination Experiment

To explore the optimum temperature, light intensityand water conditions for seed germination of Siphonostegia chinensis Benth.,seed germination experiment were carried out under different temperatures（5/15, 10/20, 15/25, 20/30℃）, different light intensity（14h light/10h darkness, complete darkness）and different concentrations（0％, 5％, 10％, 15％, 20％）of PEG-6000 solution. In terms of concentration, 5% PEG was regarded as the low level, 10% and 15% as the medium level, and 20% as the high level. The results showed that (1) Germination rate, germination potential, and germination index were increased with the rise of temperature. In addition, seed germination was significantly higher under the dark conditions than that with the 14h light/10h darkness. (2) No seed germination occurred when the temperature was below 10/20 ºC at 14h light/10h darkness. (3) Under 14h light/10h darkness, the germination rate, germination potential and germination index first increased and then decreased with the increase of PEG concentration. The low concentration was more beneficial to the seed germination. (4) Under the condition of complete darkness, the germination rate, germination potential and germination index decline with fluctuation with the increase of PEG concentration. Seed germination of Siphonostegia chinensis Benth. was inhibited in high concentration of PEG.

scholarly journals Influence of microbial priming and seeding depth on germination and growth of native wildflowers

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Daniela Barrera ◽  
Juan Luera ◽  
Kaitlynn Lavallee ◽  
Pushpa Soti
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Plant Species ◽  
Biomass Allocation ◽  
Habitat Restoration ◽  
Soil Microbes ◽  
Germination Rate ◽  
Native Soil ◽  
The Impact ◽  
Germination And Growth

Abstract Background Using native wildflowers for restoring marginal lands has gained considerable popularity. Establishment of wildflowers can be challenging due to several environmental factors. Restoring the microbial community in degraded habitats can potentially result in the native plant performance and habitat restoration. This study was conducted to investigate the impact of native soil microbes and seeding depth on germination of south Texas native wildflowers. Two wildflower species, Ratibida columnifera (Nutt.) (Mexican Hat) and Verbesina encelioides (Cav.) (cowpen daisy), were treated with microbial wash extracted from native soils, and germination rate was recorded for 14-day period. We further analyzed the growth, biomass allocation, and root colonization by mycorrhizal fungi in these two plants growing them in a plant growth chamber for 6 weeks. To determine the impact of seeding depth, we planted the seeds of the two plant species at 2-cm, 6-cm, and 12-cm depth and monitored germination and plant growth. Results The two species responded differently to the seeding depth and microbial wash treatments. Microbial wash treatment resulted in higher germination rate in R. columnifera compared to control, while it did not have any impact on V. encelioides seed germination. While microbial treatment did not influence the total biomass, it had a significant impact on the biomass allocation in both the plant species. R. columnifera seeds germinated at both 2-cm and 6-cm depth and did not germinate at 12 cm, while the V. encelioides seeds germinated only at 2 cm and did not germinate at 6-cm or 12-cm seeding depth. Conclusions While our results are species specific, our results indicate that native soil microbes can potentially improve the seed germination and growth of wildflowers. Our results also indicate the importance of specific seeding depth when sowing wildflower seeds for habitat restoration.

scholarly journals Effects of Plasma Focus on Seed Germination and Seedling Growth of 14 Thai Rice Varieties

2021 ◽  
Vol 18 (14) ◽  
Author(s):  
Vichai PURIPUNYAVANICH ◽  
Arlee TAMMAN ◽  
Piyanuch ORPONG ◽  
Roppon PICHA ◽  
Mayuree LIMTIYAYOTHIN ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Plasma Focus ◽  
Germination Rate ◽  
Growth Reduction ◽  
Rice Varieties ◽  
X Ray ◽  
Plasma Irradiation ◽  
Germination And Growth ◽  
Storage Energy

Thailand Plasma Focus 2 (TPF-2) is operated at 2.16 kJ of storage energy with argon as a filled gas and can emit sharp x-ray pulses to plant cells in nanosecond (10-9 s) duration. The effects of plasma focus x-ray on seed germination and seedling growth of 14 Thai rice varieties treated under 10 conditions; 0 (control), 2, 4, 6, 8, 10, 12, 14, 16, and 18 plasma focus shots, were investigated. The results show that the germination rates and growth reduction rates were significantly different among the rice varieties. The germination rate of 10 varieties (Jek Chuey, KDML105, LPT123, PTT1, RD41, RD47, RD49, RD67, RD79, and SPR2) decreased when treated with a high number of plasma focus shots. LD50 and GR50 values were calculated, except for KTH17, RD1, RD7, and RD31 whose germination only slightly decreased, or remained constant, even after they were treated by 18 plasma focus shots. HIGHLIGHTS Effects of plasma focus irradiation on germination and growth were assessed on 14 Thai rice varieties  Shoot and root lengths of rice seedlings were measured 10 days after of plasma treatment Plasma focus was operated at 12 kV and the number of plasma focus shots was varied from 0 (control) to 18 shots Sensitivity to plasma irradiation was found to differ among rice seeds tested GRAPHICAL ABSTRACT

scholarly journals Pengaruh Posisi Skarifikasi Benih dan Perendaman Air Kelapa terhadap Perkecambahan Biji dan Pertumbuhan Bibit Sirsak (Annona muricata L.)

1970 ◽  
Vol 5 (1) ◽  
pp. 104-112
Author(s):  
Arya Agung Pranata ◽  
Asil Barus ◽  
Meiriani
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Block Design ◽  
Dry Weight ◽  
Germination Percentage ◽  
Coconut Water ◽  
Hard Seed ◽  
Randomized Block Design ◽  
Long Time ◽  
Germination And Growth

The problem of generative propagation of soursop is its seeds take a long time to germinate which are caused by the hard seed coat. The solution to solve that problem is scarification and coconut water soaking to simply the process of imbibition and seed germination and also stimulate the growth of shoot. This objective of the research was to determine the effect of the scarification position and the soaking with various concentrations of coconut water on seed germination and growth of soursop seedling. This research was held at the Faculty of Agriculture field, the University of Sumatera Utara in March - July 2017. The research used a factorial randomized block design with two factors and three replication. The first factor is the scarification position (without scarification, stomach scarification, top scarification) and the second factor is coconut water soaking (0%; 25%; 50%; 75 %). The result of the research showed that the germination rate parameter, germination percentage, crop height, number of leaves, root volume and dry weight of crop significantly higher on without scarification treatment than with scarification treatment. The interaction between the scarification position and soaking with various coconut water concentrations was not significantly affecting the seed germination and growth of soursop seedling.

scholarly journals Effects of Exogenous Calcium on Datura Seed Germination under Drought Stress

2019 ◽  
Vol 1 (2) ◽  
Author(s):  
Yongji Wang
Keyword(s):  
Drought Stress ◽  
Seed Germination ◽  
Germination Rate ◽  
Inhibitory Effect ◽  
Control Group ◽  
High Concentration ◽  
Moisture Condition ◽  
Moderate Drought ◽  
Exogenous Calcium ◽  
Effects Of Drought

With polyethylene glycol (PEG-6000), of 0% (CK), 5%, 10%, 15%, 25% used to simulate drought stress, and CaCl2 concentration 0 (CK), of 15, 20, 25 and 30mmol/L as ion gradient of exogenous calcium, the effects of drought, exogenous calcium and the interaction between the two on the Datura seed germination, so as to explore the optimal application amount of exogenous calcium to ease the suppression of drought stress on Datura seed germination. The results showed that the germination rate, germination potential and germination index of the Datura seeds were significantly lower than those of the control group. Under the normal moisture condition, exogenous calcium of moderate and low concentration had no significant effect on the Datura seed germination, while that of high concentration showed an inhibitory effect on the seed germination. Under drought stress, with the increasing concentration of exogenous calcium, the three indicators of Datura seeds showed a trend of increasing first and then decreasing. When the exogenous calcium had the concentration of 20 mmol/L, all the indicators of seed germination reached the maximum value, while showed a downward trend when exogenous calcium concentration was 25-30 mmol/L, and even increasingly sharp with drought intensifying. Therefore, in the production and utilization of Datura, 20 mmol/L of exogenous calcium can be used to soak seeds before sowing to improve the emergence rate under low and moderate drought conditions.

scholarly journals Monitoring the viability of citrus rootstocks seeds stored under refrigeration

2013 ◽  
Vol 35 (1) ◽  
pp. 238-245 ◽  
Author(s):  
Sérgio Alves de Carvalho ◽  
Luis Fernando Carvalho Silva
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Oxygen Demand ◽  
Poncirus Trifoliata ◽  
Trifoliate Orange ◽  
Rangpur Lime ◽  
Average Value ◽  
Volkamer Lemon ◽  
Expression Rate ◽  
Germination And Growth

The citrus nursery tree is produced through the bud grafting process, in which rootstock is usually grown from seed germination. The objective of this research was to evaluate, in two dissimilar environmental conditions, the viability and polyembryony expression of five citrus rootstocks seeds stored in different periods under refrigeration. The rootstock varieties evaluated were: Rangpur lime (Citrus limonia Osb. cv. Limeira), Trifoliate orange (Poncirus trifoliata Raf. cv. Limeira), Citrumelo (P. trifoliata x C. paradisi Macf. cv. Swingle), Sunki mandarin (C. sunki Hort. ex Tanaka) and Volkamer lemon (C. volkameriana Ten. & Pasq. cv. Catania 2). The experimental design was the randomized blocks in a 11 x 5 x 2 factorial scheme, evaluating from time zero to the tenth month of storage, the five varieties of rootstock in two environments: germination and growth B.O.D type chamber (Biological Oxygen Demand - Eletrolab Brand Model FC 122) at 25 °C; and greenhouse seedbed with partial temperature control (22 °C to 36 °C) and humidity control (75-85%). The plot had 24 seeds in four replicates, using trays with substrate in greenhouse and Petri dishes with filter paper in B.O.D. chamber. The seed germination rate and polyembryony expression were evaluated monthly. It was concluded that Trifoliate and Citrumelo Swingle seeds can be stored for up to seven months, while Volkamer lemon, Rangpur lime and Sunki seeds can be stored for up to ten months. The polyembryony expression rate was slightly higher when measured in greenhouse than in B.O.D. chamber and remained stable in both environments until the seventh month, from which dropped sharply. Citrumelo Swingle seeds expressed the highest polyembryony rate (18.8%), followed by Rangpur lime and Volkamer lemon (average value of 13.7%), Sunki (9.4%) and Trifoliate (3.2%). Despite some differences among varieties, the viability of rootstock stored seeds can be monitored either in the greenhouse or in B.O.D. germination chamber, the latter being the faster and more suitable method.

scholarly journals Urinary reproductive hormones influence seed germination within diluted urine of heifers: alternative pregnancy diagnostic method

2020 ◽  
Vol 46 (1) ◽  
pp. 8090-8099
Author(s):  
Iva Lázničková ◽  
Tamara Fedorova ◽  
Magdaléna Štolcová ◽  
Anna Kubátová
Keyword(s):  
Seed Germination ◽  
Dilution Rate ◽  
Mung Bean ◽  
Germination Rate ◽  
Diagnostic Techniques ◽  
Reproductive Hormones ◽  
Diagnostic Methods ◽  
Urine Samples ◽  
Wheat Germination ◽  
Germination And Growth

Experimental non-invasive pregnancy diagnostic techniques, such as seed germination testing in diluted urine, may serve as an alternative to standardized pregnancy diagnostic methods, but scientific validation of these methods is required. This study aimed to use Czech Fleckvieh heifers to investigate the influence of urinary reproductive hormones (oestrone sulphate, 17β-oestradiol, and pregnanediol3-glucuronide) on the germination success of seeds placed within their diluted urine, and further to verify the reliability of seed germination test for pregnancy diagnosis in this species. Mung bean and wheat seeds were germinated in two urine-water dilutions (1:4 and 1:14) for three days, using urine samples obtained from either pregnant or non-pregnant heifers. Germinated seeds were counted daily, and the shoot lengths were measured three days after placement in the urine. Levels of urinary reproductive hormones were determined using competitive heterogeneous enzyme immunoassays (EIAs). Despite the dilution rate used, final results indicated that the urine from pregnant heifers inhibited germination and growth in mung beans only. On the contrary, wheat germination rate was higher within the urine from pregnant females on days 1 and 2, when using a 1:14 dilution rate. For both seed species, correlations between urinary hormone profiles and seed germination parameters were found, depending on the day of the experiment and the rate of dilution used. The shoot lengths of mung bean seeds were significantly (P < 0.05) and negatively correlated with the profiles of all hormones analysed from the urine samples. Thus, urinary reproductive hormones influence seed germination and growth rate of wheat and mung beans, indicating the potential for this technique to be used for pregnancy determination in heifers.

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