scholarly journals Urinary reproductive hormones influence seed germination within diluted urine of heifers: alternative pregnancy diagnostic method

2020 ◽  
Vol 46 (1) ◽  
pp. 8090-8099
Author(s):  
Iva Lázničková ◽  
Tamara Fedorova ◽  
Magdaléna Štolcová ◽  
Anna Kubátová
Keyword(s):  
Seed Germination ◽  
Dilution Rate ◽  
Mung Bean ◽  
Germination Rate ◽  
Diagnostic Techniques ◽  
Reproductive Hormones ◽  
Diagnostic Methods ◽  
Urine Samples ◽  
Wheat Germination ◽  
Germination And Growth

Experimental non-invasive pregnancy diagnostic techniques, such as seed germination testing in diluted urine, may serve as an alternative to standardized pregnancy diagnostic methods, but scientific validation of these methods is required. This study aimed to use Czech Fleckvieh heifers to investigate the influence of urinary reproductive hormones (oestrone sulphate, 17β-oestradiol, and pregnanediol3-glucuronide) on the germination success of seeds placed within their diluted urine, and further to verify the reliability of seed germination test for pregnancy diagnosis in this species. Mung bean and wheat seeds were germinated in two urine-water dilutions (1:4 and 1:14) for three days, using urine samples obtained from either pregnant or non-pregnant heifers. Germinated seeds were counted daily, and the shoot lengths were measured three days after placement in the urine. Levels of urinary reproductive hormones were determined using competitive heterogeneous enzyme immunoassays (EIAs). Despite the dilution rate used, final results indicated that the urine from pregnant heifers inhibited germination and growth in mung beans only. On the contrary, wheat germination rate was higher within the urine from pregnant females on days 1 and 2, when using a 1:14 dilution rate. For both seed species, correlations between urinary hormone profiles and seed germination parameters were found, depending on the day of the experiment and the rate of dilution used. The shoot lengths of mung bean seeds were significantly (P < 0.05) and negatively correlated with the profiles of all hormones analysed from the urine samples. Thus, urinary reproductive hormones influence seed germination and growth rate of wheat and mung beans, indicating the potential for this technique to be used for pregnancy determination in heifers.

scholarly journals Inducing salt tolerance in sweet corn by magnetic priming

2017 ◽  
Vol 109 (1) ◽  
pp. 89 ◽  
Author(s):  
Soheil Karimi ◽  
Saeid ESHGHI ◽  
Saeid KARIMI ◽  
Saman HASAN-NEZHADIAN
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Plant Growth ◽  
Sweet Corn ◽  
Germination Rate ◽  
Nacl Stress ◽  
Proline Accumulation ◽  
Membrane Thermostability ◽  
Germination And Growth ◽  
Magnetic Priming

<p>This study evaluates seed germination and growth of sweet corn under NaCl stress (0, 50, and 100 mM), after exposing the seeds to weak (15 mT) or strong (150 mT) magnetic fields (MF) for different durations (0, 6, 12, and 24 hours). Salinity reduced seed germination and plant growth. MF treatments enhanced rate and percentage of germination and improved plant growth, regardless of salinity. Higher germination rate was obtained by the stronger MF, however, the seedling were more vigorous after priming with 15 mT MF. Proline accumulation was observed in parallel with the loss of plant water content under 100 mM NaCl stress. MF prevented proline accumulation by improving water absorption. Positive correlation between H<sub>2</sub>O<sub>2</sub> accumulation and membrane thermostability (MTI) was found after MF treatments, which revealed that MF primed the plant for salinity by H<sub>2</sub>O<sub>2</sub> signaling. However, over-accumulation of H<sub>2</sub>O<sub>2</sub> after prolonged MF exposure adversely affected MTI under severe salt stress. In conclusion, magnetic priming for 6 hours was suggested for enhancing germination and growth of sweet corn under salt stress.</p>

scholarly journals Influence of microbial priming and seeding depth on germination and growth of native wildflowers

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Daniela Barrera ◽  
Juan Luera ◽  
Kaitlynn Lavallee ◽  
Pushpa Soti
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Plant Species ◽  
Biomass Allocation ◽  
Habitat Restoration ◽  
Soil Microbes ◽  
Germination Rate ◽  
Native Soil ◽  
The Impact ◽  
Germination And Growth

Abstract Background Using native wildflowers for restoring marginal lands has gained considerable popularity. Establishment of wildflowers can be challenging due to several environmental factors. Restoring the microbial community in degraded habitats can potentially result in the native plant performance and habitat restoration. This study was conducted to investigate the impact of native soil microbes and seeding depth on germination of south Texas native wildflowers. Two wildflower species, Ratibida columnifera (Nutt.) (Mexican Hat) and Verbesina encelioides (Cav.) (cowpen daisy), were treated with microbial wash extracted from native soils, and germination rate was recorded for 14-day period. We further analyzed the growth, biomass allocation, and root colonization by mycorrhizal fungi in these two plants growing them in a plant growth chamber for 6 weeks. To determine the impact of seeding depth, we planted the seeds of the two plant species at 2-cm, 6-cm, and 12-cm depth and monitored germination and plant growth. Results The two species responded differently to the seeding depth and microbial wash treatments. Microbial wash treatment resulted in higher germination rate in R. columnifera compared to control, while it did not have any impact on V. encelioides seed germination. While microbial treatment did not influence the total biomass, it had a significant impact on the biomass allocation in both the plant species. R. columnifera seeds germinated at both 2-cm and 6-cm depth and did not germinate at 12 cm, while the V. encelioides seeds germinated only at 2 cm and did not germinate at 6-cm or 12-cm seeding depth. Conclusions While our results are species specific, our results indicate that native soil microbes can potentially improve the seed germination and growth of wildflowers. Our results also indicate the importance of specific seeding depth when sowing wildflower seeds for habitat restoration.

scholarly journals Effects of Plasma Focus on Seed Germination and Seedling Growth of 14 Thai Rice Varieties

2021 ◽  
Vol 18 (14) ◽  
Author(s):  
Vichai PURIPUNYAVANICH ◽  
Arlee TAMMAN ◽  
Piyanuch ORPONG ◽  
Roppon PICHA ◽  
Mayuree LIMTIYAYOTHIN ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Plasma Focus ◽  
Germination Rate ◽  
Growth Reduction ◽  
Rice Varieties ◽  
X Ray ◽  
Plasma Irradiation ◽  
Germination And Growth ◽  
Storage Energy

Thailand Plasma Focus 2 (TPF-2) is operated at 2.16 kJ of storage energy with argon as a filled gas and can emit sharp x-ray pulses to plant cells in nanosecond (10-9 s) duration. The effects of plasma focus x-ray on seed germination and seedling growth of 14 Thai rice varieties treated under 10 conditions; 0 (control), 2, 4, 6, 8, 10, 12, 14, 16, and 18 plasma focus shots, were investigated. The results show that the germination rates and growth reduction rates were significantly different among the rice varieties. The germination rate of 10 varieties (Jek Chuey, KDML105, LPT123, PTT1, RD41, RD47, RD49, RD67, RD79, and SPR2) decreased when treated with a high number of plasma focus shots. LD50 and GR50 values were calculated, except for KTH17, RD1, RD7, and RD31 whose germination only slightly decreased, or remained constant, even after they were treated by 18 plasma focus shots. HIGHLIGHTS Effects of plasma focus irradiation on germination and growth were assessed on 14 Thai rice varieties  Shoot and root lengths of rice seedlings were measured 10 days after of plasma treatment Plasma focus was operated at 12 kV and the number of plasma focus shots was varied from 0 (control) to 18 shots Sensitivity to plasma irradiation was found to differ among rice seeds tested GRAPHICAL ABSTRACT

scholarly journals Pengaruh Posisi Skarifikasi Benih dan Perendaman Air Kelapa terhadap Perkecambahan Biji dan Pertumbuhan Bibit Sirsak (Annona muricata L.)

1970 ◽  
Vol 5 (1) ◽  
pp. 104-112
Author(s):  
Arya Agung Pranata ◽  
Asil Barus ◽  
Meiriani
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Block Design ◽  
Dry Weight ◽  
Germination Percentage ◽  
Coconut Water ◽  
Hard Seed ◽  
Randomized Block Design ◽  
Long Time ◽  
Germination And Growth

The problem of generative propagation of soursop is its seeds take a long time to germinate which are caused by the hard seed coat. The solution to solve that problem is scarification and coconut water soaking to simply the process of imbibition and seed germination and also stimulate the growth of shoot. This objective of the research was to determine the effect of the scarification position and the soaking with various concentrations of coconut water on seed germination and growth of soursop seedling. This research was held at the Faculty of Agriculture field, the University of Sumatera Utara in March - July 2017. The research used a factorial randomized block design with two factors and three replication. The first factor is the scarification position (without scarification, stomach scarification, top scarification) and the second factor is coconut water soaking (0%; 25%; 50%; 75 %). The result of the research showed that the germination rate parameter, germination percentage, crop height, number of leaves, root volume and dry weight of crop significantly higher on without scarification treatment than with scarification treatment. The interaction between the scarification position and soaking with various coconut water concentrations was not significantly affecting the seed germination and growth of soursop seedling.

scholarly journals Monitoring the viability of citrus rootstocks seeds stored under refrigeration

2013 ◽  
Vol 35 (1) ◽  
pp. 238-245 ◽  
Author(s):  
Sérgio Alves de Carvalho ◽  
Luis Fernando Carvalho Silva
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Oxygen Demand ◽  
Poncirus Trifoliata ◽  
Trifoliate Orange ◽  
Rangpur Lime ◽  
Average Value ◽  
Volkamer Lemon ◽  
Expression Rate ◽  
Germination And Growth

The citrus nursery tree is produced through the bud grafting process, in which rootstock is usually grown from seed germination. The objective of this research was to evaluate, in two dissimilar environmental conditions, the viability and polyembryony expression of five citrus rootstocks seeds stored in different periods under refrigeration. The rootstock varieties evaluated were: Rangpur lime (Citrus limonia Osb. cv. Limeira), Trifoliate orange (Poncirus trifoliata Raf. cv. Limeira), Citrumelo (P. trifoliata x C. paradisi Macf. cv. Swingle), Sunki mandarin (C. sunki Hort. ex Tanaka) and Volkamer lemon (C. volkameriana Ten. & Pasq. cv. Catania 2). The experimental design was the randomized blocks in a 11 x 5 x 2 factorial scheme, evaluating from time zero to the tenth month of storage, the five varieties of rootstock in two environments: germination and growth B.O.D type chamber (Biological Oxygen Demand - Eletrolab Brand Model FC 122) at 25 °C; and greenhouse seedbed with partial temperature control (22 °C to 36 °C) and humidity control (75-85%). The plot had 24 seeds in four replicates, using trays with substrate in greenhouse and Petri dishes with filter paper in B.O.D. chamber. The seed germination rate and polyembryony expression were evaluated monthly. It was concluded that Trifoliate and Citrumelo Swingle seeds can be stored for up to seven months, while Volkamer lemon, Rangpur lime and Sunki seeds can be stored for up to ten months. The polyembryony expression rate was slightly higher when measured in greenhouse than in B.O.D. chamber and remained stable in both environments until the seventh month, from which dropped sharply. Citrumelo Swingle seeds expressed the highest polyembryony rate (18.8%), followed by Rangpur lime and Volkamer lemon (average value of 13.7%), Sunki (9.4%) and Trifoliate (3.2%). Despite some differences among varieties, the viability of rootstock stored seeds can be monitored either in the greenhouse or in B.O.D. germination chamber, the latter being the faster and more suitable method.

scholarly journals Effects of Zn2+ Stress on Seed Germination and growth of seedling

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Wenbin Liu ◽  
Aizhen Shao ◽  
Chuanying Wang
Keyword(s):  
Seed Germination ◽  
Chlorophyll Content ◽  
Root Length ◽  
Stress Condition ◽  
Zinc Concentration ◽  
Germination Rate ◽  
Germination Index ◽  
Stem Length ◽  
Zinc Fertilizer ◽  
Germination And Growth

The effects of Zn2+ stress on seed germination, seedling growth and chlorophyll content were studied in order to better understand the effect of heavy metal Zn on the growth and development of green plants. The concentration gradient of Zn2+ was 20, 50,100,150,200,300,500,700mg / L, and deionized water was used as control. The results showed that under the Zn2+ stress condition, the germination index of the rhubarb seeds increased with the increase of Zn2+ concentration. Germination potential, germination rate and germination index were the highest when Zn2+concentration was 100mg / L, the conductivity was the lowest at zinc concentration of 100mg / L, the root length, stem length and chlorophyll content of Zn2+ gradually reduced. The results showed that the amount of Zn2+ could promote seed germination, but the root length, bud length and chlorophyll content of seedlings could be affected by different degrees. The zinc fertilizer should be used in the production.

scholarly journals Effect of CuSO4 on the growth of Brassica juncea L.

2020 ◽  
Vol 10 (5) ◽  
pp. 245-247
Author(s):  
M.R. Pratik ◽  
B. Bavi ◽  
B. Bavi
Keyword(s):  
Seed Germination ◽  
Brassica Juncea ◽  
Copper Sulfate ◽  
Copper Sulphate ◽  
Germination Rate ◽  
High Concentration ◽  
Black Mustard ◽  
Germination And Growth

Seeds of Black Mustard (Brassica juncea) were sowed in a paper cup with copper sulfate at different concentration. This experiment aimed to determine the effect of the variation in concentration of copper sulphate on the germination and growth of seeds of Brassica juncea. We wanted to establish which is the highest concentration of copper that the seeds of Black Mustard can tolerate. Seedlings growth investigation and measurements were made after 5 days. The seed germination rate was low to no for the high concentration and for control that increased dramatically with the decrease in concentration. At high concentration, no growth was seen.

Effects of Biogas Slurry Pretreatment on Germination and Seedling Growth of Vicia faba L.

2014 ◽  
Vol 955-959 ◽  
pp. 208-212 ◽  
Author(s):  
Yan Zhao ◽  
Qing Song Yang ◽  
Shu Yang ◽  
Hong Mei Zhao ◽  
Qing Song Duan ◽  
...  
Keyword(s):  
Seed Germination ◽  
Vicia Faba ◽  
Seedling Growth ◽  
Germination Rate ◽  
Germination Index ◽  
Biogas Slurry ◽  
Seed Soaking ◽  
Germination Potential ◽  
Faba Beans ◽  
Germination And Growth

In order to study the effect of seed soaking with biogas slurry on seed germination and growth of faba beans (Vicia faba L.), the experiment was conducted. The seeds were soaked in biogas slurry for various soaking durations (0, 2, 4, 6 and 8 hours) and different concentration (0, 25, 50, 75 and 100 %) along with control to determine the optimal soaking duration and find out the best growing condition. The highest germination rate, germination potential and germination index were obtained when the faba beans seeds soaked for 4 hours in 75 % biogas slurry. Seedling growth was recorded in plants where seeds soaked for 6 hours in 75 % biogas slurry. It was concluded that the seed soaking of biogas slurry with lower concentration (25, 50 and 75 %) showed best results regarding maximum germination and enhanced growth. However, under the higher concentration (100 %) conditions of biogas slurry had a tendency of decrease on seed germination and seedling growth.

scholarly journals Isolation and Identification of Some Biocontrol Fungi and Evaluation of Their Efficacy Against Charchol Rot Disease Caused by Macrophomina phaseolina on Mung Bean

2021 ◽  
Vol 39 (3) ◽  
pp. 197-203
Author(s):  
Abdel Nabi Matrood ◽  
◽  
Hala Abdel Jaber Abdulhassan ◽  
Mohamad Imad Khrieba ◽  
Mountaser Adam Mohamad Amin ◽  
...  
Keyword(s):  
Seed Germination ◽  
Disease Severity ◽  
Mung Bean ◽  
Fungal Pathogen ◽  
Pathogenic Fungus ◽  
Germination Rate ◽  
Macrophomina Phaseolina ◽  
Bean Seed ◽  
Charcoal Rot ◽  
Rot Disease

This study aimed to isolate and identify Macrophomina phaseolina fungus which was the causal aget of Charcol rot disease on mung bean (Vigna radita Wilczek L) from Elgadisia Governorate in order to isolate, diagnose, and evaluate efficacy of biological control fungal agents isolalated from the rhizosphere of the host plant. The biocontrol agents T. koningii, C. globosum and A. carbonarius were effective in inhibiting the pathogenic fungus under laboratory and greenhouse conditions. Two isolates of M. phaseolina fungal pathogen were identified in most samples of infected mung bean. Pathogenicity tests showed the ability of M. phaseolina to attack the seeds and cause seed rot. Isolates 1 and 2 of the fungal pathogen reduced the seed germination rate to 71.56 and 63.75%, respectively, compared with 86.66% for the control. The results obtained indicated the presence of a significant difference between the two isolates in inducing seedlings damping-off which reached 40% and 30%, respectively, compared with 0% for the control. The disease severity of isolate 1 reached 75.3% and that of isolate 2 reached 63.6%. The inhibitory effect of the fungal antagonists T. koningii and C. globosum varied. A. carbonarius demonstrated level 3 of inhibition to the pathogenic fungus M. phaseolina, whereas C. globosum and T. koningii demostrated inhibitory levels of 2 and 1, respectively. Results obtained also indicated that treatments with antagonistic fungi T. koningii, C. globosum and A. carbonarius increased the growth parameters of the mung bean plant. The antagonistic fungus C. globosum increased the plant height, root fresh and dry weight and chlorophyll content compared with the control. Furthermore, the results showed that treatment with T. koningii increased the mung bean seed germination rate to 100% and decreased disease severity of mung bean charcoal rot disease to 45.88% as compared to 89.86% severity for the contol. Likewise, the other antagonistic fungi A. carbonarius and C. globosum also increased the mung bean seed germination , and decreased mung bean charcoal rot disease severity. Keywords : Charcoal rot disease, mung bean, Macrophomina phaseolina, T. koningii, C. globosum, A. carbonarius.

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