scholarly journals Isolation of Lactobacillus species from fermented Parkia biglobosa seed and screening for their probiotic activity

2021 ◽  
Vol 13 (Volume 13, Issue 2) ◽  
pp. 212-216
Author(s):  
K. Kuti ◽  
I.M. Hussaini ◽  
A. Usman ◽  
A. Isa
Keyword(s):  
Antibacterial Activity ◽  
Low Ph ◽  
Lactobacillus Species ◽  
Biochemical Tests ◽  
Antimicrobial Substances ◽  
Probiotic Potential ◽  
Gram Staining ◽  
Colonial Morphology ◽  
Lactobacillus Spp

Abstract. Food fermentation has been a tradition for decades due to its immense benefits. Lactic acid bacteria are known to possess probiotic potential due to various chemical antimicrobial substances they produce. This research was aimed at isolating Lactobacillus spp. from locally fermented locust beans and screening the isolates for their probiotic potential. A total of 20 samples of fermented locust beans (P. biglobosa) were collected and processed. The samples were inoculated onto de-Mann Rogosa Sharpe (MRS) media and incubated anaerobically. The isolates with characteristic colonial morphology of Lactobacillus sp. were characterized using Gram staining and biochemical tests. Lactobacillus spp. isolates were screened in-vitro for probiotic potential using the following parameters, tolerance of low pH, bile tolerance and antimicrobial activity. Four isolates of Lactobacillus species consisting of Lactobacillus fermentum and Lactobacillus plantarum were isolated from different fermented locust beans. All isolates showed tolerance to low pH and bile concentration of 3.04 and 0.3%, respectively. The Lactobacillus species isolates showed varying antibacterial activity against all test bacteria with isolate S06 (L. plantarum) showing the highest degree of antibacterial activity. In conclusion, the Lactobacillus species isolated from fermented locust beans can serve as probiotic candidate.

IN VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY AND PROBIOTIC POTENTIAL OF LACTOBACILLUS STRAINS AGAINST SOME HUMAN PATHOGENS

2018 ◽  
pp. 31-35
Keyword(s):  
Antimicrobial Activity ◽  
Bacterial Species ◽  
Acid Tolerance ◽  
Lactobacillus Species ◽  
Human Pathogens ◽  
Probiotic Potential ◽  
Probiotic Strains ◽  
Potential Methods ◽  
Lactobacillus Spp

Objective: Curd and yogurt are the major source of probiotic consumed by majority of the Indian population and Lactobacillus spp. are the predominant bacterial species in them. The present study was carried out to isolate Lactobacillus spp, from locally available dairy products and assess for the probiotic potential. Methods: The isolated were identified using biochemical assays and assessed for the properties such as Acid tolerance, Bile tolerance and NaCl tolerance. The identified strains were also subjected to antibiotic susceptibility and Antimicrobial activity using standard methodology. Results: A total of ten Lactic acid bacteria were isolated form different sources, belonging to different Lactobacillus species viz. L. acidophilus IAMb-3, L. brevis IAMb-6, L. bulgaricus IAMb-9, L. lactis IAMb-10, L. acidophilus IAMb-11, L. fermentum IAMb-13, L. casei IAMb-14, L. casei IAMb-15, L. plantarum IAMb-16 and L. rhamonsus IAMb-18. These identified strains were able to tolerate pH-3, 0.3% bile salt and upto 5-6% NaCl. Seven out of ten stains had antimicrobial activity against the pathogenic strain used in the study. Conclusion: In the present study the some of the potential probiotic strains have been isolated from local commercial and homemade sources. The isolated strains were capable of tolerating the harsh similar to human gut environment and found antagonistic to gut pathogens, hence can considered as potential probiotics.

scholarly journals In vitro assessment of antibacterial activity from Lactobacillus spp. strains against virulent Salmonella species isolated from slaughter animals in Benin

2019 ◽  
Vol 12 (12) ◽  
pp. 1951-1958
Author(s):  
Alidehou Jerrold Agbankpe ◽  
Tamegnon Victorien Dougnon ◽  
Roubaya Balarabe ◽  
Esther Deguenon ◽  
Lamine Baba-Moussa
Keyword(s):  
Antibacterial Activity ◽  
Breast Milk ◽  
Lactobacillus Delbrueckii ◽  
Salmonella Spp ◽  
Promising Alternative ◽  
Probiotic Potential ◽  
Virulent Strains ◽  
In Vitro Antibacterial Activity ◽  
Lactobacillus Spp

Background and Aim: Salmonella spp. are among the world's leading foodborne pathogens, found naturally in the intestines of many animals. Lactic acid bacteria, mainly Lactobacillus, are a promising alternative to antibiotics for animal and human health. This study aimed to assess the in vitro antibacterial activity of Lactobacillus spp. strains against virulent Salmonella spp. isolated from slaughter animals in Benin. Materials and Methods: Eleven samples of raw cow's milk, five samples of breast milk, and six infant stool samples were taken. From these samples, strains of Lactobacillus were isolated and identified. The probiotic potential of each of the identified strains was characterized, and finally in vitro antibacterial activity of these strains was evaluated against three virulent strains of Salmonella spp. and a reference strain of Salmonella Typhimurium ATCC 14028. Results: Out of the 22 samples collected, 20 strains of Lactobacillus spp. were isolated and identified. These strains included Lactobacillus plantarum (30%), Lactobacillus delbrueckii (25%), Lactobacillus casei (25%), Lactobacillus salivarius (15%), and Lactobacillus acidophilus (05%). Characterization of the probiotic potential of these strains showed that only 16 strains were resistant to pH=1.5. Fourteen of them were able to withstand the simulated gastric juice (pH 1.5+pepsin). The 14 probiotic strains showed very good antibacterial activity against virulent strains of Salmonella spp. with inhibition zone diameters ranging from 12.36±0.03 mm to 35.33±0.05 mm (R values>6 mm). Conclusion: From this study, Lactobacillus strains isolated from raw cow milk, breast milk, and infantile stool might be used as some valid candidates for probiotics. It also represents good alternatives for antibiotics in the fight against animal and human salmonellosis.

scholarly journals Bacteriocin-mediated inhibition of some common pathogens by wild and mutant Lactobacillus species and in vitro amplification of bacteriocin encoding genes

ADMET & DMPK ◽  
2021 ◽  
Author(s):  
Arooj Ahsan ◽  
Bushra Mazhar ◽  
Muhammad Kamran Khan ◽  
Madiha Mustafa ◽  
Muhammad Hammad ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Diffusion Method ◽  
Lactobacillus Species ◽  
Lactobacillus Curvatus ◽  
Biochemical Tests ◽  
Specific Primers ◽  
Gram Staining ◽  
Salmonella Enteritis ◽  
Encoding Genes

Lactobacilli are the most common probiotics used in food and other industries because of their capability of producing bacteriocins. Bacteriocins are compounds that are used to kill pathogenic microorganisms. As most bacteria have become resistant to synthetic antibacterial tools, the importance of using probiotics as antibacterial agents has increased. This work was done to check the bacteriocin effect on some common pathogens and the influence of mutation on the bacteriocin activity of Lactobacilli was also investigated. Four strains were isolated, identified from meat and pickles samples via culturing methods, staining, biochemical tests, and ribotyping. Preliminary tests, including Gram staining and catalase test, were done for the confirmation of Lactobacillus species. All strains were gram-positive and catalase-negative. Antibacterial activity was checked against Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus thuringiensis, Escherichia coli, and Salmonella enteritis via agar well diffusion method. The mutations were done using ethidium bromide and the influence of wild and mutants were also checked. Interestingly, mutants developed more virulence than wild ones. It was also observed that they all were sensitive to pepsin. Protein estimation was done via Bradford method. Ribotyping of GCU-W-PS1 revealed 99 % homology with Lactobacillus plantarum and GCU-W-MS1 to Lactobacillus curvatus (99 % homology). Curvacin A, sakacin P, and plantaricin A genes were also amplified using specific primers. Gene sequence showed the presence of curvacin A gene in GCU-W-MS1. It was concluded that lactic acid bacteria could be used as antibacterial tools against common pathogens.

scholarly journals Antibacterial activity of lactobacillus species isolated from raw goat milk against Staphylococcus aureus

2021 ◽  
Vol 6 (2) ◽  
pp. 065-072
Author(s):  
Emeka Okechukwu ◽  
Joy Nkeiruka Dike-Ndudim ◽  
Chizaram Winners Ndubueze ◽  
Charlotte Chinwendu Iwuji-Aguzie
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Growth Inhibition ◽  
Test Organism ◽  
Goat Milk ◽  
Diffusion Method ◽  
Lactobacillus Species ◽  
Biochemical Tests ◽  
Lactobacillus Spp ◽  
Agar Well Diffusion Method

Lactobacillus spp. are known to have many health benefits and are mostly used as probiotics. They can cause inhibition of certain micro-organisms by the reduction of pH due to production of lactic acid, hydrogen peroxide and antimicrobial compounds such as bacteriocins. The antibacterial; activity of Lactobacillus spp. Against Staphylococcus aureus was evaluated using agar well diffusion method. Three raw goat milk samples were pooled together, serially diluted, cultured on MRS (De Man, Rogosa, Sharpe) Agar, and purified. Four Lactobacillus species were isolated and identified by biochemical tests as L. lactis, L. plantarum, L. Casei and L. Fermentum. Antibacterial activity of the four isolates against the test organism, Staphylococcus aureus, was determined by agar well diffusion method following the extraction of crude and precipitated bacteriocin from the isolates. All the isolates produced antagonistic effects against the test organism with maximum zones of growth inhibition produced by precipitated bacteriocin [14.0mm (L. casei) to 16.0mm (L. lactis)] whereas minimum zones of growth inhibition was produced by crude bacteriocin [8.0mm (L. casei) to 11.0mm (L. lactis)]. The results obtained confirmed that the four Lactobacilli isolated have growth inhibitory effects against Staphylococcus aureus and can therefore serve as important probiotic organism. It also goes further to show the importance of Lactobacillus bacteriocins as food bio-preservatives. This therefore justifies an in-depth investigation for the identification and application of these organisms in generating alternative antibacterial therapies for human and animal diseases to counter the problem of bacteria antibiotic resistance in recent years.

scholarly journals Antibiotic sensitivity and in vitro antimicrobial activity of plant extracts to pseudomonas fluorescens isolates collected from diseased fish

1970 ◽  
Vol 1 (4) ◽  
pp. 82-88 ◽  
Author(s):  
MJ Foysal ◽  
MM Rahman ◽  
M Alam
Keyword(s):  
Antibacterial Activity ◽  
Pseudomonas Fluorescens ◽  
Plant Extracts ◽  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Leaf Extracts ◽  
Sensitivity Pattern ◽  
Medicinal Plant Extracts

Studies were conducted to identify Pseudomonas fluorescens isolates from a collection of bacteria isolated from bacterial haemorrhagic septicaemia infected carp and catfish, evaluate their antibiotic sensitivity pattern and screen the antibacterial activity of some medicinal plant extracts against the isolates.. A total of 10 isolates were identified as P. fluorescens by morphological, physiological and biochemical tests. In vitro antibiotic sensitivity test of the P. fluorescens isolates were conducted by disc diffusion method for seven antibiotics where, all of the isolates were found to be sensitive only against streptomycin and gentamycin but, most of the isolates (80%) were found resistant to chloramphenicol (C). Moreover, eighty percent of the isolates showed resistance to multiple antibiotics. A total of 118 plant extracts were screened for their antibacterial activity against the P. fluorescens isolates where the isolates exhibited sensitivity to 30 samples. Leaf extracts of Tamarindus indicus, Terminalia chebula, Citrus aurantifolia, Eugenia caryophyllata and Spondias pinnata were found to inhibit the growth of all of the P. fluorescens isolates. DOI: http://dx.doi.org/10.3329/ijns.v1i4.9733 IJNS 2011 1(4): 82-88

scholarly journals Use of indigenous beneficial bacteria (Lactobacillus spp.) as probiotics in shrimp (Penaeus monodon) aquaculture

2018 ◽  
Vol 5 (1) ◽  
pp. 127-135 ◽  
Author(s):  
Fatema Ahmmed ◽  
Mirja Kaizer Ahmmed ◽  
Md Saifuddin Shah ◽  
Ghausiatur Reza Banu
Keyword(s):  
Penaeus Monodon ◽  
Challenge Test ◽  
Tolerance Test ◽  
Antagonistic Effect ◽  
In Vitro Test ◽  
Biochemical Tests ◽  
Probiotic Treatment ◽  
Vibrio Spp ◽  
Lactobacillus Spp

The present study was conducted to study in-vitro the antagonistic effect of Lactobacillus spp. against the pathogenic bacterial vibrio harveyi on shrimp. For this purpose, shrimp samples were collected from three different Ghers at Batiaghata upazilla, Khulna. Gills and intestines were taken out from the samples to identify the load of Lactobacillus spp. and Vibrio spp. The results revealed that the load of Lactobacillus spp. was found more than Vibrio spp. both in gills and intestines; the gills also contained higher load of Vibrio spp. than in the intestines. V. harveyi was separated from the isolated Vibrio spp. with different types of biochemical tests: Gram stain, Motility test, Indole test, VP test, MR test, Arginine dihydrolase, Salt tolerance test, growth at different temperature ranges and colony color on TCBS agar media. The isolated V. harveyi was subjected for in-vitro test. In in-vitro challenge test, the potential antagonistic effect of Lactobacillus spp. against V. harveyi was gradually obtained at 0, 4th, 8th, 12th hour of treatments. Interesting finding was that, with the time, the load of V. harveyi was reduced gradually and the lowest load was obtained after 12 hours of probiotic inoculation. The present study revealed an excellent in-vitro antagonistic probiotic effect of Lactobacillus spp. on V. harveyi. Therefore the result suggested that probiotic treatment might be an effective alternative to the use of antibiotics in treatments of bacterial diseases in shrimp aquaculture.Res. Agric., Livest. Fish.5(1): 127-135, April 2018

HISTOLOGICAL STUDY OF LETTUCE AND BASIL BY INFECTION WITH PS. AERUGINOSA AND PS. FLUORESCENS IN VITRO

2020 ◽  
pp. 56-69
Author(s):  
G.V. GODOVA ◽  
◽  
A.A. OVOD ◽  
N.V. ASTAKHOVA ◽  
E.A. KALASHNIKOVA ◽  
...  
Keyword(s):  
Virulent Strain ◽  
Histological Study ◽  
Plant Cells ◽  
Ultrastructural Changes ◽  
Cytotoxic Action ◽  
Biochemical Tests ◽  
Potential Threat ◽  
Gram Staining ◽  
Electronic Microscope

Studies based on the use of an electronic microscope revealed ultrastructural changes in plant cells of lettuce and Basil in interaction with Ps.aeruginosa ATCC B3994, expressed in deformation and violation of the integrity of the cell wall, increasing the size of chloroplasts, sometimes full destruction accompanied by the increased number of plastoglobuli. Ps.fluorescens ATСC948 were localized in the cytoplasm and chloroplasts of cells without a cytotoxic action. Plant isolates were identified with gram staining, biochemical tests and PCR. Contamination of non-heattreated leaf vegetables with virulent strain Ps. aeruginosa is a potential threat to human health and life.

Isolation and screening of Lactobacillus species from dogs for probiotic action

2017 ◽  
Author(s):  
N. Mohammad Sharif ◽  
B. Sreedevi ◽  
R. K. Chaitanya ◽  
D. Sreenivasulu
Keyword(s):  
Antibacterial Activity ◽  
16S Rrna ◽  
Cell Surface Hydrophobicity ◽  
Lactobacillus Species ◽  
Nucleotide Sequence Analysis ◽  
Rrna Gene ◽  
Acid And Bile Tolerance ◽  
In Vitro Antibacterial Activity ◽  
Pcr Targeting

The present study aimed at the detection of suitable Lactobacillus species from dogs for usage as probiotic. A total of 67 rectal swabs from healthy pups were analyzed and 49 (73.1%) Lactobacillus isolates were identified based on morphological, biochemical characteristics and confirmed by genus specific PCR targeting 16S rRNA gene. A total of 20 isolates that showed strong aggregation, high cell surface hydrophobicity, acid and bile tolerance were screened for in-vitro antibacterial activity by agar well diffusion assay, where prominent inhibitory zones were observed against majority of the test pathogens. Reduction in antibacterial activity was noticed after neutralization, proteinase K and heat treatment of supernatants. Partial 16S rRNA nucleotide sequence analysis of seven Lactobacillus isolates that showed prominent in-vitro antibacterial activity revealed maximum sequence homology with Lactobacillus fermentum (for six isolates) and Lactobacillus agilis (for one isolate). The in-vitro antibacterial activity results highlighted the need for in-vivo studies in India to establish probiotic potential of canine faecal Lactobacillus species in the near future.

scholarly journals In vitro probiotic potential of Lactobacillus spp. isolated from fermented milks

2013 ◽  
Vol 65 (6) ◽  
pp. 1876-1882 ◽  
Author(s):  
A.F. Cunha ◽  
L.B. Acurcio ◽  
B.S. Assis ◽  
D.L.S. Oliveira ◽  
M.O. Leite ◽  
...  
Keyword(s):  
Bile Salts ◽  
Quality Standards ◽  
Gastric Ph ◽  
Multiple Resistance ◽  
Pathogenic Microorganisms ◽  
Indicator Microorganisms ◽  
Probiotic Potential ◽  
Belo Horizonte ◽  
Lactobacillus Spp

The potential of in vitro probiotic Lactobacillus spp. was evaluated in fermented milks marketed in Belo Horizonte, MG, Brazil. Of the samples analyzed, 86.7% had at least 10(6) CFU/mL of Lactobacillus spp., complying with the Brazilian quality standards for fermented milks. Furthermore, 56.7% had minimum count ranging from 10(8) to 10(9) CFU/mL, which is in accordance with legal parameters. The remaining 43.3% would not be able to satisfactorily guarantee benefits to consumers. The amount of Lactobacillus spp. varied between batches of products, which may indicate failures in monitoring during manufacture, transport or storage. All strains of Lactobacillus spp. showed some inhibitory activity against the indicator microorganisms, being more pronounced against pathogenic microorganisms than against non-pathogenic (P<0.05). Samples of Lactobacillus spp. showed different profiles of antimicrobial susceptibility, with an occurrence of cases of multidrug resistance. All strains tested showed sensitivity to bile salts (0.3%) and resistance to gastric pH (2.0). Lactobacillus spp. of commercial fermented milks should be present in higher amounts in some brands, be resistant to bile salts and have no multiple resistance to antimicrobials.

scholarly journals Ιn Vitro Screening of γ-Aminobutyric Acid and Autoinducer-2 Signalling in Lactic Acid Bacteria Exhibiting Probiotic Potential Isolated from Natural Black Conservolea Olives

Foods ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 640 ◽  
Author(s):  
Foteini Pavli ◽  
Eleni Gkana ◽  
Oluwabunmi Adebambo ◽  
Kimon-Andreas Karatzas ◽  
Efstathios Panagou ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Growth Conditions ◽  
Low Ph ◽  
Aminobutyric Acid ◽  
Bile Salt Hydrolase ◽  
Haemolytic Activity ◽  
Autoinducer 2 ◽  
Probiotic Potential ◽  
Bile Salt Hydrolase Activity

In the present study, 33 strains of lactic acid bacteria (LAB) previously isolated from natural black Conservolea olives were assessed for their probiotic potential in vitro, as well as for their autoinducer-2 (AI-2) activity under standard growth conditions and the production of γ-aminobutyric acid (GABA). The probiotic tests included the in vitro resistance to low pH and resistance to bile salts, the evaluation of bile salt hydrolase activity, as well as safety tests regarding their possible haemolytic activity and their antimicrobial activity against pathogens. The results indicated that 17 strains were able to survive in low pH and in the presence of bile, with 15 of them also exhibiting partial bile salt hydrolase activity. None of the strains exhibited haemolytic activity or inhibited the growth of any of the examined pathogens. Moreover, the strains displayed generally low AI-2 activity under the growth conditions tested, regardless of the species. Interestingly, in contrast to what has been found in most foods, none of the isolates were found to produce GABA after 48 h of growth. The results from the AI-2 activity and extracellular GABA detection were considered as unexpected for LAB with probiotic attributes.

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