Correlation between maize genotypes and the stalk rot caused by maize Fusarium

2007 ◽  
Vol 55 (4) ◽  
pp. 447-452 ◽  
Author(s):  
C. Szőke ◽  
T. Árendás ◽  
F. Rácz ◽  
J. Pintér ◽  
E. Nagy ◽  
...  
Keyword(s):  
Natural Infection ◽  
Female Parent ◽  
Weather Conditions ◽  
Artificial Inoculation ◽  
Resistance Level ◽  
Stalk Rot ◽  
Maize Genotypes ◽  
Female Component ◽  
Different Levels ◽  
Single Cross Hybrids

Six single-cross hybrids and their parental lines were inoculated with the FG36 Fusarium graminearum isolate in 2005 and 2006. In both years the degree of infection increased after artificial inoculation for both the hybrids and the inbred lines compared with the level of natural infection. The more severe stalk infection recorded in 2005 than in 2006 could be attributed to the weather conditions during flowering and harvesting.The 18 genotypes examined exhibited different levels of resistance to fusarium stalk rot. It could be concluded from the results that the resistance level of the female parent was decisive in the inheritance of the response to fusarium stalk rot (female component-hybrid r = 0.88, male component-hybrid r = 0.39).Some genotypes may be severely affected in epidemic years, while exhibiting a lower rate of infection in years with lower pathogen pressure. This suggests that successful breeding for resistance can only be carried out efficiently by means of artificial inoculation.

scholarly journals Studies on the Fusarium stalk rot infection of the maize genotypes using the Findex percentage and a computerised image analysis program

2011 ◽  
pp. 45-51
Author(s):  
Csaba Szőke ◽  
István Virág ◽  
Donát Magyar ◽  
Ferenc Rácz ◽  
Csaba L. Marton
Keyword(s):  
Image Analysis ◽  
Natural Infection ◽  
Grain Filling ◽  
Inbred Lines ◽  
Stalk Rot ◽  
Maize Genotypes ◽  
Accurate Picture ◽  
Computerised Image Analysis ◽  
Continental Climate ◽  
Plot Design

In a continental climate, the pathogens causing the most serious problems are species belonging to the Fusarium genus. When the pathogen attacks the stalk, the plant dies earlier, reducing grain filling and resulting in small, light ears. In addition, the stalks break or lodge, resulting in further yield losses from ears that cannot be harvested. During the three years of the experiment, 14 inbred lines were examined. The genotypes were sown in a two-factor split-plot design with four replications, with the genotypes in the main plots and four treatments in the subplots: two Fusarium graminearum isolates (1. FG36, 2. FGH4), 3. sterile kernels, 4. untreated control. The results experiments showed significant differences between the genotypes for resistance to fusarium stalk rot. Among the inbred lines the best resistance to fusarium stalk rot was exhibited by P06 and P07, both of which were related to ISSS. The precision and sensitivity of disease evaluations carried out visually and using image analysis software were compared in the experiment, and with two exceptions the CV values were lower for the image analysis. As the CV for measurements can be considered as a relative error, it can be stated that image analysis is the more precise of the two methods, so this technique gives a more accurate picture of the extent of stalk rot. The extent of stalk rot developing in response to natural infection is extremely environment-dependent, so the use of artificial inoculation is recommended for selection trials. 

Selection of cocoa clones for resistance to Phytophthora palmivora by artificial inoculation and natural infection in the field

2021 ◽  
Author(s):  
Vanusa Rodrigues de Souza ◽  
Edna Dora Martins Newman Luz ◽  
José Luis Pires ◽  
Marcos Vinicius Oliveira dos Santos ◽  
Elisângela dos Santos ◽  
...  
Keyword(s):  
Natural Infection ◽  
Artificial Inoculation ◽  
Phytophthora Palmivora ◽  
Selection Of

THE GROWTH PATTERN OF CORN IN RELATION TO RESISTANCE TO ROOT AND STALK ROT

1965 ◽  
Vol 43 (10) ◽  
pp. 1277-1283 ◽  
Author(s):  
R. E. Wall ◽  
C. G. Mortimore
Keyword(s):  
Growth Pattern ◽  
Dry Matter ◽  
Dry Weight ◽  
High Ratio ◽  
Vegetative Tissue ◽  
Physiological Maturity ◽  
Stalk Rot ◽  
Pith Tissue ◽  
Single Cross ◽  
Single Cross Hybrids

Single-cross hybrids resistant to root and stalk rot generally had greater growth rates, leaf areas, and stalk densities than susceptible single crosses. Differences in dry weight of tops were evident by the time of silk emergence and these differences became more pronounced as the hybrids approached maturity. Susceptible hybrids were characterized by a cessation of vegetative growth at pollination and a rapid senescence of leaves at or shortly after physiological maturity. Hybrids resistant to root and stalk rot were characterized by a continued increase in vegetative dry matter for several weeks after pollination, resulting in relatively heavy lower stalks and a high ratio of vegetative tissue to grain. The density of pith tissue from the lower stalk at physiological maturity was negatively correlated with subsequent stalk rot incidence.

scholarly journals KETAHANAN DELAPAN KULTIVAR TEMBAKAU LOKAL BONDOWOSO TERHADAP TIGA PATOGEN PENTING (Ralstonia solanacearum, Pectobacterium carotovorum, DAN Phytophthora nicotianae)

2020 ◽  
Vol 18 (3) ◽  
pp. 89
Author(s):  
TITIEK YULIANTI ◽  
NURUL HIDAYAH ◽  
SRI YULAIKAH
Keyword(s):  
Ralstonia Solanacearum ◽  
Block Design ◽  
Production Quality ◽  
Phytophthora Nicotianae ◽  
Pectobacterium Carotovorum ◽  
Resistance Level ◽  
Stalk Rot ◽  
Randomized Block Design ◽  
Tobacco Production ◽  
Screen House

<p>ABSTRAK<br />Tembakau bondowoso merupakan tembakau lokal rajangan yang<br />berkembang di Kabupaten Bondowoso, Jawa Timur. Saat ini ada delapan<br />kultivar dengan karakter produksi, mutu, dan ketahanannya terhadap<br />penyakit yang berbeda. Layu bakteri (Ralstonia solanacearum), busuk<br />batang berlubang (Pectobacterium carotovorum), dan lanas (Phytophthora<br />nicotianae) merupakan penyakit yang sering menyebabkan turunnya<br />produksi tembakau bondowoso. Evaluasi ketahanan delapan kultivar<br />tembakau bondowoso (Samporis, Serumpung, Marakot, Samporis Lokal,<br />Samporis AH, Samporis CH, Samporis B. Disbun, dan Deli) terhadap<br />ketiga patogen tersebut dilaksanakan di laboratorium dan rumah kasa Balai<br />Penelitian Tanaman Pemanis dan Serat (Balittas) mulai bulan April sampai<br />dengan Oktober 2011. Penelitian terhadap ketiga patogen tersebut<br />dilakukan secara terpisah. Masing-masing kultivar ditanam sebanyak 10<br />tanaman, 1 tanaman/polibag. Setiap perlakuan (kultivar) diulang 3 kali dan<br />disusun dalam rancangan acak kelompok (RAK). Inokulasi R.<br />solanacearum dan P. carotovorum dilakukan secara terpisah 24 jam<br />sebelum transplanting. Inokulasi P. nicotianae dilakukan dengan dua cara,<br />yaitu melalui akar dan pangkal batang. Inokulasi akar sama dengan cara<br />inokulasi bakteri. Inokulasi pangkal batang dilakukan pada tanaman<br />berumur 2 minggu setelah transplanting. Pengamatan intensitas penyakit<br />dilakukan setiap minggu selama 11 minggu. Hasil penelitian menunjukkan<br />bahwa kultivar Samporis CH, Samporis, dan Deli tahan terhadap P.<br />carotovorum, R. solanacearum, dan P. nicotianae. Kultivar Samporis CH.,<br />Samporis, dan Deli ketahanannya lebih tinggi terhadap ketiga patogen,<br />dengan intensitas penyakit berkisar antara 3,3%-6,7%. Kultivar Marakot<br />sangat rentan terhadap ketiga patogen tersebut dengan tingkat keparahan ≥<br />50%. Demikian pula kultivar Samporis AH yang rentan terhadap R.<br />solanacearum, P. nicotianae dan P. carotovorum dengan intensitas<br />penyakit 23,3-53,3%. Oleh karena itu, kultivar Samporis CH, Samporis,<br />dan Deli cocok dikembangkan pada lahan endemik penyakit tular tanah di<br />Kabupaten Bondowoso.<br />Kata kunci: tembakau  bondowoso, Pectobacterium  carotovorum<br />Phytophthora  nicotianae, Ralstonia  solanacearum,<br />ketahanan</p><p>ABSTRACT<br />Bondowoso tobacco is a local type of sliced tobacco which is<br />restrictedly cultivated in Bondowoso Regency, East Java. There are eight<br />cultivars known, ie. Samporis, Serumpung, Marakot, Samporis Lokal,<br />Samporis AH, Samporis CH, Samporis B. Disbun, and Deli with their own<br />distinctive characters on their production, quality, and resistance to<br />diseases. Bacterial wilt (Ralstonia solanacearum), hollow stalk rot<br />(Pectobacterium carotovorum), and blackshank (Phytophthora nicotianae<br />are the main cause of bondowoso tobacco production loss. Evaluation on<br />the resistance level of the cultivars to the three pathogens above has been<br />conducted at a laboratory and screen house scale in Indonesian Sweetener<br />and Fibre Crops Research Institute from April to October 2011. The<br />evaluation of each pathogen was conducted separately. Each evaluation of<br />the pathogen per cultivar used 10 plants planted individually in a polybag.<br />The experiment was arranged in a randomized block design with 3<br />replicates. R. solanacearum and P. carotovorum were separately<br />inoculated on the test plants 24 h before transplanting. The inoculation of<br />P. nicotianae was done twice via the root and stem. Disease intensity was<br />observed weekly for 11 weeks. The results showed that Samporis CH,<br />Samporis, and Deli cultivars were resistant to P. carotovorum, R.<br />solanacearum and P. nicotianae, whereas Samporis and Deli cultivars<br />were more resistant to the pathogens (disease intensity ranged 3.3-6.7%).<br />Marakot cultivar was very susceptible to all of the three pathogens (disease<br />intensity ≥ 50%). Similarly, Samporis AH cultivar was also susceptible to<br />the pathogens with disease intensity ranged 23.3-53.3%. The study<br />indicated that Samporis CH, Samporis, and Deli cultivars are suitable to be<br />cultivated in the endemic soil born pathogen areas of Bondowoso<br />Regency.<br />Key words: bondowoso  tobacco, Pectobacterium  carotovorum,<br />Phytophthora  nicotianae, Ralstonia  solanacearum,<br />resistance</p>

scholarly journals A Novel Source of Resistance to Tomato Yellow Leaf Curl Virus Exhibiting a Symptomless Reaction to Viral Infection

1998 ◽  
Vol 123 (6) ◽  
pp. 1004-1007 ◽  
Author(s):  
M. Friedmann ◽  
M. Lapidot ◽  
S. Cohen ◽  
M. Pilowsky
Keyword(s):  
Natural Infection ◽  
Tomato Yellow Leaf ◽  
Yellow Leaf ◽  
Susceptible Parent ◽  
Partial Dominance ◽  
Disease Symptoms ◽  
Leaf Curl Virus ◽  
Very High ◽  
Different Levels ◽  
Leaf Curl

Tomato yellow leaf curl virus (TYLCV), transmitted by the tobacco whitefy (Bemisia tabaci Genn.), can be devastating to tomato (Lycopersicon esculentum L.) crops in tropical and subtropical regions. The development of resistant cultivars is the best option for control of TYLCV. However, all the available resistant commercial cultivars tested at the Volcani Center, when inoculated with TYLCV, developed different levels of disease symptoms. In this study, we report the development of a breeding line, TY172, which is a symptomless carrier of TYLCV. Line TY172, whether infected in the greenhouse with viruliferous whiteflies, or when grown in the field under natural infection, showed no symptoms of the disease. Viral DNA was detected in infected TY172 plants, albeit at much lower levels than a susceptible infected control. In addition, grafting experiments using infected susceptible scions grafted onto TY172 stocks, showed that even when exposed continuously to very high levels of virus, line TY172 did not develop disease symptoms, nor did it accumulate high levels of the virus. When TY172 was crossed with susceptible lines, the hybrids exhibited milder symptoms and lower viral content than the susceptible parent, yet higher than that of TY172, suggesting a partial dominance for the TY172 resistance. Upon inoculation of F2 populations, the amount of symptomless individuals appeared in a ratio of≈7:64. This suggests that at least three genes may account for the resistance.

scholarly journals Genetic Testing of Inbred Lines and Single Cross Hybrids against Fusarium Stalk Rot Caused by Fusarium moniliforme in Maize (Zea mays L.)

2021 ◽  
Vol 10 (11) ◽  
pp. 352-361
Author(s):  
R. Archana H. C. Lohithaswa ◽  
R. Pavan B. N. Swathi ◽  
N. Mallikarjuna
Keyword(s):  
Zea Mays ◽  
Genetic Testing ◽  
Fusarium Moniliforme ◽  
Zea Mays L ◽  
Inbred Lines ◽  
Yield Potential ◽  
Disease Score ◽  
Stalk Rot ◽  
Single Cross ◽  
Single Cross Hybrids

Globally, Maize (Zea mays L.) is a third major cereal food crop. It is a multipurpose crop with 26% of its production is used as food by human beings. Maize is known as “queen of cereals”, because of its high genetic yield potential, efficient utilization of radiant energy and wider adaptability. About 65 different phytopathogens affect the maize production in different stages of life cycle. Among which Fusarium moniliforme is one such soil borne pathogen causes Fusarium stalk rot (FSR) disease that ultimately reduces maize yield potential over the world. In any breeding program, screening and genetic testing of available germplasm resources against pathogens is necessary to prevent yield losses. Hence, the present research screened around 114 maize inbred lines and 45 single cross hybrids (SCHs) against FSR under artificial epiphytotic conditions. Among 114 inbreds, only four inbreds viz., CM 202, 10878, MAI-759 and MAI-766 (mean disease score of 3-4) showed moderately resistant reaction and out of 45 SCHs, only one hybrid combination i.e., MAI329 × CM202 (mean disease score was 2.60) exhibited resistance reaction against Fusarium stalk rot. Nevertheless, these resistance sources could be utilized in maize breeding programs for obtaining high yielding cultivars with resistance towards FSR disease.

scholarly journals Evaluation of efficacy of modern fungicides against fusarium head blight on winter wheat

2019 ◽  
pp. 51-59
Author(s):  
M. Dzham
Keyword(s):  
Winter Wheat ◽  
Fusarium Head Blight ◽  
Weather Conditions ◽  
Artificial Inoculation ◽  
Head Blight ◽  
Protection Measures ◽  
Growing Seasons ◽  
Biological Preparation ◽  
Significant Difference ◽  
Untreated Check

Goal. To study the influence of modern biological and chemical preparations on the development of fungi of the genus Fusariumunder conditions of artificial inoculation. Methods. The efficacy of fungicides was studied in 2018—2019 in the Zhytomyr region, Pulinsky district under the conditions of artificial inoculation on winter wheat of the Bogdan variety. Mixture of conidia of the fusarium fungi (F. graminearum, F. culmorum, F. poae, F. avenaceum) was used for ear inoculation. The plot area was 2 m2 in four replications, the plot placement was randomized. The inoculation of winter wheat ears was carried out at the phase of mass flowering. At the phase of milky-wax maturity, records were taken to determine the spread and development of the disease. The following fungicides were used to protect wheat crops: Folicur BT EC, 1.2 l/ha, Magnello 350 EC, 1.0 l/ ha, Haupsin, 0.2 l/ha, Fitodoktor, 0.05 kg/ha, Alliot 250 EC, 0.5 l/ ha, Bumper Super EC, 1.2 l/ha and mixtures of Magnello 350 EC, 0.75 l/ha + Haupsin 0.2 l/ha, Magnello350 EC, 0.75 l/ha + Fitodoktor, 0.05 kg/ha. Results. The results obtained during the years of investigationsindicate that the used preparations are efficient for the protection of crops from fusarium head blight on winter wheat. However,not all fungicides tested had high enough efficacy. The most effective against the pathogens F.graminearum, F. culmorum, F. poae, F. avenaceumunder conditions of Zhytomyr region were fungicides Folicur BT EC, 1.2 l/ha, Magnello 350 EC, 1.0 l/ha and mixtures of fungicide and biological preparation (Magnello 350 EC, 0.75 l/ ha + Haupsin, 0.2 l/ha). Efficacy of fungicides used against these pathogens ranged from 69.2% to 75.2%. After fungicide application the weight of 1000 grains increased by 7.3—13.6 g. Significant difference between experimental variants and untreated check on 1000 grain weight was noted. With the use of fungicides, the yield increased by 22.3—24.8%. Conclusions. The analysis of the protection against fusarium head blight convinces the need to apply chemical protection measures when weather conditions are favorable for the development of the disease. In the growing seasons of 2018—2019, under conditions of the Zhytomyr region, the most promising and effective against the pathogens F.graminearum, F. culmorum, F. poae, F. avenaceum were fungicides Folicur BT EC, 1.2 l/ha, Magnello 350 EC, 1.0 l/ ha and mixtures of fungicide and biological preparation Magnello 350 EC, 0.75 l/ ha + Haupsin, 0.2 l/ha.

scholarly journals Development and Release of Two Mid-Late Maturing Maize Hybrids for the Highland Agro-Ecologies of Uganda

2020 ◽  
Vol 7 (3) ◽  
pp. 115-124
Author(s):  
Frank Kagoda ◽  
Daniel B. Kwemoi ◽  
Lawrence Owere ◽  
Mildred Ochwo-Ssemakula ◽  
Godfrey Asea
Keyword(s):  
Maize Yield ◽  
Inbred Lines ◽  
Pests And Diseases ◽  
Input Use ◽  
Cropping Season ◽  
Maize Genotypes ◽  
Single Cross ◽  
Highland Maize ◽  
Yield Trials ◽  
Single Cross Hybrids

The maize yield in the highland areas of Uganda (1500 to 3000 masl), rarely exceeding 2 t ha-1. The use of unimproved maize genotypes, the prevalence of pests and diseases, and low levels of input use has tremendously contributed to the low grain yields.  Accordingly, we obtained 100 maize inbred lines majorly from CIMMYT in the year 2013. The inbred lines were evaluated for adaptability and those selected were intercrossed to generate 80 single cross hybrids by January 2015. The 80 single cross hybrids were evaluated in preliminary yield trials in three sites namely, Kachwekano, Kalengyere and Buginyanya in 2015a season. The best combiners were crossed with the selected males to obtain 122 three-way cross hybrids. These were evaluated for adaptability in advanced yield trials in 2016a in Kachwekano, Kalengyere and Bukwo, from where the best five hybrids (BH33, BH31, BH27, BH104 and BH38) were selected for National Performance Trials (NPT)  in 2016b and 2017a seasons in Bukwo, Buginyanya, Zombo, Fort portal and Kalengere. Two candidate varieties namely BH31 and BH33 exhibited good agronomic qualities such as earlier maturity, tolerance to MLN and other diseases. The selected hybrids yielded 8.2 t ha-1 and 7.2 t ha-1 for BH31 and BH33, respectively. The two varieties are appropriate for cultivation in major highland and transitional maize growing regions in Uganda and were subsequently released as NAROMAIZE 731 and NAROMAIZE 733, respectively. The newly released varieties are expected to reduce the highland maize cropping season from over six months to five months.

scholarly journals Response of Barley Genotypes to Fusarium Head Blight under Natural Infection and Artificial Inoculation Conditions

2021 ◽  
Vol 37 (5) ◽  
pp. 455-464
Author(s):  
Raja Khanal ◽  
Thin Meiw Choo ◽  
Allen G. Xue ◽  
Bernard Vigier ◽  
Marc E. Savard ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Prince Edward Island ◽  
Natural Infection ◽  
Spring Barley ◽  
Artificial Inoculation ◽  
High Yield ◽  
Conidial Suspension ◽  
Head Blight ◽  
One Year ◽  
Barley Genotypes

Forty-eight spring barley genotypes were evaluated for deoxynivalenol (DON) concentration under natural infection across 5 years at Harrington, Prince Edward Island. These genotypes were also evaluated for Fusarium head blight (FHB) severity and DON concentration under field nurseries with artificial inoculation of <i>Fusarium graminearum</i> by the grain spawn method across 2 years at Ottawa, Ontario, and one year at Hangzhou, China. Additionally, these genotypes were also evaluated for FHB severity under greenhouse conditions with artificial inoculation of <i>F. graminearum</i> by conidial suspension spray method across 3 years at Ottawa, Ontario. The objective of the study was to investigate if reactions of barley genotypes to artificial FHB inoculation correlate with reactions to natural FHB infection. DON concentration under natural infection was positively correlated with DON concentration (<i>r</i> = 0.47, <i>P</i> < 0.01) and FHB incidence (<i>r</i> = 0.56, <i>P</i> < 0.01) in the artificially inoculated nursery with grain spawn method. Therefore, the grain spawn method can be used to effectively screen for low DON. FHB severity, generated from greenhouse spray, however, was not correlated with DON concentration (<i>r</i> = 0.12, <i>P</i> > 0.05) under natural infection and it was not correlated with DON concentration (<i>r</i> = -0.23, <i>P</i> > 0.05) and FHB incidence (<i>r</i> = 0.19, <i>P</i> > 0.05) in the artificially inoculated nursery with grain spawn method. FHB severity, DON concentration, and yield were affected by year, genotype, and the genotype × year interaction. The effectiveness of greenhouse spray inoculation for indirect selection for low DON concentration requires further studies. Nine of the 48 genotypes were found to contain low DON under natural infection. Island barley had low DON and also had high yield.

scholarly journals Dependence of pea root mass distribution on weather conditions under varying levels of phosphorus application

2018 ◽  
Vol 32 (3) ◽  
pp. 365-372 ◽  
Author(s):  
Agnieszka Klimek-Kopyra ◽  
Krzysztof Rębilas
Keyword(s):  
Mass Distribution ◽  
Water Availability ◽  
Soil Depth ◽  
Weather Conditions ◽  
Root Mass ◽  
Pea Root ◽  
Application Rates ◽  
Phosphorus Application ◽  
Very High ◽  
Different Levels

Abstract The aim of the study was to describe the distribution of pea root mass in the soil, over a three-year period, under varying weather conditions and at different levels of phosphorus application, by means of evaluating and comparing parameters of a mathematical model characterising cumulative root mass distribution. A two-factor experiment was conducted in Prusy, near Krakow: the first factor was the level of phosphorus application (0-70-140 kg P2O5 ha−1) and the second was the cultivars (six cultivars were tested). Experimental data produced using the model indicated that the root distribution was strongly differentiated by water availability in the years of the study. This appeared in some cases to be a more important factor than phosphorus application rates. The estimated soil depth at which 50% of the root mass was accumulated differed significantly for the dry and the wet year. In the wet year, only very high phosphorus application rates contributed to an increase in root mass distribution. The estimation of root mass distribution from the presented data can be used to improve phosphorus application depending on the amount of precipitation.

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