scholarly journals COMPARATIVE STUDY OF DIFFERENT METHODS FOR DOG SEMEN CRYOPRESERVATION AND TESTING UNDER CLINICAL CONDITIONS

2000 ◽  
Vol 48 (3) ◽  
pp. 325-333 ◽  
Author(s):  
F. Szász ◽  
G. Gábor ◽  
László Solti
Keyword(s):  
Clinical Practice ◽  
Sperm Quality ◽  
Intrauterine Insemination ◽  
Fluorescein Isothiocyanate ◽  
Final Conclusion ◽  
Peanut Agglutinin ◽  
Semen Cryopreservation ◽  
Average Litter Size ◽  
Acrosome Integrity ◽  
Clinical Conditions

The extenders and freezing rates from three different freezing protocols were combined and compared to each other in order to study the post-thawing acrosome integrity and fertility of frozen dog sperm. A commercial bovine TRIS-base extender (TRILADYL) and two self-made canine semen extenders (Norwegian and Dutch) were combined with a conventional bovine and two canine freezing regimes, and acrosome integrity of frozen/thawed spermatozoa was assessed by fluorescein isothiocyanate conjugated peanut agglutinin staining (FITC-PNA). Differences between freezing/thawing protocols were reflected in the proportion of cells with acrosomal damage and not based on motility results. It was concluded that during dog semen cryopreservation extenders had less influence on the post-thawing sperm quality than did the freezing rates. The optimal extender/freezing rate combination (TRILADYL/Norwegian) was used in the clinical practice to evaluate the fertility of frozen sperm administered by intrauterine insemination using a surgical approach. The pregnancy rate was 57% (4/7), but the average litter size was low (2.8). This may have been due to the insufficient sperm numbers contained in an insemination dose and/or to the incorrect timing of artificial insemination (AI). The final conclusion is that the commercial bovine extender is useful for freezing dog semen, and the TRILADYL/Norwegian freezing protocol is recommended as the most advantageous combination for the freezing of canine semen in the clinical practice.

scholarly journals Antioxidants Significantly Improve Qinling Giant Panda (Ailuropoda Melanoleuca Qinlingensis) Sperm Quality During Cryopreservation

2021 ◽  
Author(s):  
Ruixue Zhang ◽  
Hemeng Dong ◽  
Pengpeng Zhao ◽  
Chunmei Shang ◽  
Hang Qi ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Laminaria Japonica ◽  
Mitochondrial Activity ◽  
Ailuropoda Melanoleuca ◽  
Lycium Barbarum ◽  
Giant Pandas ◽  
Semen Cryopreservation ◽  
Freezing Medium ◽  
Acrosome Integrity

Abstract Background: Semen cryopreservation has become an essential tool for the reproduction and long-term preservation of giant pandas (Ailuropoda melanoleuca). However, cryopreservation is severely detrimental to sperm quality, including motility, plasma membrane, acrosome integrity, and mitochondrial activity. It is necessary to screen a effective antioxidants for improvement of sperm quality. Eight groups of antioxidants were added to the freezing medium.Results:The results showed that lycium barbarum polysaccharide (LBP), Laminaria japonica polysaccharides (LJP), or goujresveratrol (RSV) added to the freezing medium significantly improved sperm motility, acrosome integrity, and mitochondrial activity during the cryopreservation process. Furthermore, the activities of glutathione and superoxide dismutase were also improved. However, the levels of reactive oxygen species and malondialdehyde in semen were notably reduced. Regarding fertility, acrosin activity was significantly increased in LBP-treated sperm. Unfortunately, sperm viability, DNA fragmentation, and hyaluronidase activity were not significantly different between the above groups. Conclusions: LBP (2.0 mg/mL) or RSV (50 μM) are the best candidate antioxidants for inclusion in the freezing medium for improving the quality of panda spermatozoa, during semen cryopreservation.

scholarly journals Improvement in Sperm Parameters With Traditional Iranian Remedy

2016 ◽  
Vol 22 (2) ◽  
pp. 223-226 ◽  
Author(s):  
Farnaz Sohrabvand ◽  
Somaye Mahroozade ◽  
Sodabe Bioos ◽  
Seyed Mohammad Nazari ◽  
Fataneh Hashem Dabaghian
Keyword(s):  
Male Infertility ◽  
Traditional Medicine ◽  
Assisted Reproductive Technology ◽  
Intracytoplasmic Sperm Injection ◽  
Sperm Quality ◽  
Intrauterine Insemination ◽  
Sperm Count ◽  
Reproductive Technology ◽  
Sperm Parameters ◽  
Medicinal Treatment

Introduction. Idiopathic male infertility is a global problem with almost no definite medicinal treatment. Most patients have to go through intrauterine insemination or assisted reproductive technology for achieving fertility. Unfortunately, success rates are low in cases with very low sperm count. Therefore it seems that improvement in sperm quality can have beneficial effects on assisted reproductive technology outcome. Case Report. A 39-year-old man with history of infertility for 6 years was referred to the traditional medicine clinic with a recurrent unsuccessful intracytoplasmic sperm injection trial. His sperm analysis showed severe oligoasthenoteratozoospermia. After taking a traditional remedy he had a remarkable improvement in his sperm parameters, which led to the formation of 8 embryos in the following intracytoplasmic sperm injection cycle. Conclusion. Traditional medicine presents various food and remedy options for treating male infertility. It seems that combination therapy can be beneficial in obtaining better results in treatment of male idiopathic infertility.

scholarly journals Clinical Practice of Hepatorenal Syndrome: A Brief Review on Diagnosis and Management

2021 ◽  
Vol 3 (2) ◽  
pp. 1-7
Author(s):  
. Rendy ◽  
. Febyan ◽  
Krisnhaliani Wetarini
Keyword(s):  
Clinical Practice ◽  
Hepatorenal Syndrome ◽  
Organ Transplant ◽  
Kidney Injury ◽  
Therapeutic Measures ◽  
Clinical Conditions ◽  
Underlying Pathophysiology

The hepatorenal syndrome is one of various potential causes of acute kidney injury in patients with decompensated liver disease. Hepatorenal syndrome is diagnosed based on reducing kidney function without any evidence of intrinsic kidney disease, including proteinuria, hematuria, or abnormal kidney ultrasonography. Clinically, hepatorenal syndrome is divided into two types named type 1 and type 2. The most favorable therapy for HRS cases is liver transplantation; however, only a few undergo this procedure due to the high mortality. Other modalities for hepatorenal syndrome therapy are pharmacology and non-pharmacology approaches. The purpose of management HRS is to optimize and stabilize the patient until an organ transplant available. This review aims to discuss the underlying pathophysiology and demonstrate the diagnostic approach of hepatorenal syndrome to determine the most appropriate therapeutic measures in clinical practice. The clinicians must be aware of management principles of hepatorenal syndrome to improve the quality of care for patients and optimize the clinical conditions.

scholarly journals Systematic evaluation of CdSe/ZnS Quantum Dots toxicity on the reproduction and offspring health in male BALB/c mice

2020 ◽  
Author(s):  
Li Li ◽  
Xiaotan Lin ◽  
Tingting Chen ◽  
Kan Liu ◽  
Zhiwen Yang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Body Weight ◽  
Early Stage ◽  
Sperm Quality ◽  
Systematic Evaluation ◽  
High Dose ◽  
Male Mice ◽  
Organ Index ◽  
Acrosome Integrity ◽  
Offspring Health

Abstract Background: Increased applications of quantum dots (QDs) in biomedical field have aroused attention for their potential toxicological effects. Although many studies have been carried out on the toxicity of QDs, their effects on reproductive and development are still unclear. In this study, we systematically evaluated the male reproductive toxicity and developmental toxicity of CdSe/ZnS QDs in BALB/c mice.Results: The male mice were injected intravenously with CdSe/ZnS QDs at the dosage of 2.5 mg/kg BW or 25 mg/kg BW, respectively, and the survival status, biodistribution of QDs in testes, serum sex hormone levels, histopathology, sperm motility and acrosome integrity was measured on Day 1, 7, 14, 28 and 42 after injection. On Day 35 after treatment, male mice were housed with non-exposed female mice, and then offspring number, body weight, organ index and histopathology of major organs, blood routine and biochemical tests of offspring were measured to evaluate the fertility and offspring health. The results showed that CdSe/ZnS QDs could rapidly distribute in the testis, and the fluorescence of QDs could still be detected on Day 42 post-injection. QDs had no adverse effect on the structure of testis and epididymis, but high-dose QDs could induce apoptosis of Leydig cells in testis at an early stage. No significant differences in survival of state, body weight organ index of testis and epididymis, sex hormones levels, sperm quality, sperm acrosome integrity and fertility of male mice were observed in QDs exposed groups. However, the development of offspring was obviously influenced, which was mainly manifested in the slow growth of offspring, changes of organ index of main organs, and the abnormality of liver and kidney function parameters.Conclusion: Our findings revealed that CdSe/ZnS QDs were able to cross the blood-testis barrier (BTB), produce no discernible toxic effects on the male reproductive system, but could affect the healthy growth of future generations to some extent. In view of the broad application prospect of QDs in biomedical fields, our findings might provide insight into the biological safety evaluation of the reproductive health of QDs.

Neurologist, 30 years’ experience, Italy

2020 ◽  
pp. 257-259
Author(s):  
Markus Reuber ◽  
Gregg H. Rawlings ◽  
Steven C. Schachter
Keyword(s):  
At Risk ◽  
Clinical Practice ◽  
Case Reports ◽  
Epileptic Seizures ◽  
Correct Treatment ◽  
Risk Of Death ◽  
Convulsive Seizures ◽  
Clinical Conditions

This chapter assesses how, in clinical practice, the diagnostic and therapeutic borders between epileptic and Psychogenic Non-Epileptic Seizures (PNES) are ill-defined and sometimes contrast with the schematic views offered by scientific reports. It specifically considers two case reports that illustrate how it is difficult to distinguish the two clinical conditions at the time of the diagnosis and, when the diagnosis is apparently clear, to start the correct treatment. The first patient was referred with seizures characterized by “convulsions” accompanied by loss of awareness. His seizures started when he was caught in an emergency and was at risk of death. The second patient, a 24-year-old girl, was hospitalized because she had experienced “convulsive” seizures. She received contrasting opinions regarding her diagnosis.

scholarly journals Techniques for sperm evaluation using fluorescent probes

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4365
Author(s):  
Andrielle Thainar Mendes Cunha ◽  
José Oliveira Carvalho ◽  
Margot Alves Nunes Dode
Keyword(s):  
Fluorescent Probes ◽  
Fluorescent Microscopy ◽  
Membrane Integrity ◽  
Fluorescein Isothiocyanate ◽  
Mitochondrial Potential ◽  
Sperm Analysis ◽  
Merocyanine 540 ◽  
Plasmatic Membrane ◽  
Chromatin Integrity ◽  
Acrosome Integrity

A variety of laboratory tests were developed to obtain more reliable results of sperm evaluation and increase the accuracy of sperm fertility predictions. These tests detected damage of sperm specific compartments or organelles, which cannot be detected in routine sperm analysis. The use of fluorescent probes and detection using fluorescent microscopy or flow cytometry is an important tool but a more precise and accurate laboratory test is needed. Propidium iodide and 6-carboxyfluorescein diacetate are used for evaluations of plasmatic membrane integrity. Fluorescein isothiocyanate, associated with conjugated lecithin Psium sativum or Arachis hypogaea, are used for evaluations of acrosome integrity. Two probes, MitoTracker or Rhodamine123, are generally used to measure the absence or presence of mitochondrial potential. However, a better option is 5,5’; 6,6’ - tetrachloro - 1,1’; 3,3’ -tetraetilbenzimidazolil-carbocyanine (JC-1) dye, which assesses not only the presence of mitochondrial potential and distinguished spermatozoa with poorly and highly functional mitochondria. Two techniques, TUNEL or COMETA, and the Acridine Orange Test (AOT) dye are used to evaluate chromatin integrity. A fluorescence technique based on chlortetracycline (CTC) or Merocyanine 540 is used to estimate whether sperm pass by or through the capacitation process. This review focuses on the fluorescent probes that are most widely used to evaluate plasma membrane integrity, capacitation, acrosome integrity, chromatin integrity and mitochondrial potential.

scholarly journals Effect of whole and clarified egg yolk based extenders on post-thaw semen quality in bulls.

2021 ◽  
Author(s):  
Prosper Kamusasa ◽  
Eddington Gororo ◽  
Fungayi Primrose Chatiza
Keyword(s):  
Semen Quality ◽  
Sperm Quality ◽  
Egg Yolk ◽  
Quality Parameters ◽  
Normal Morphology ◽  
Semen Cryopreservation ◽  
Bull Semen ◽  
Inclusion Level ◽  
Whole Egg

Abstract This study was conducted to evaluate the comparative cryoprotective effects of whole egg yolk and clarified egg yolk on post thaw sperm quality parameters and to determine the optimum clarified egg yolk inclusion level (10-20%) in semen extenders for Mashona bull semen cryopreservation. It was shown that there was a significant decrease in sperm quality variables following cryopreservation. Semen quality increased with the concentration of clarified egg yolk, indicating a positive relationship between egg yolk LDL concentration and maintenance of in vitro sperm quality. The 20% clarified egg yolk (CEY20) extender treatment gave post-thaw motility, viability and normal morphology values which were comparable to the control (20% whole egg yolk, WEY20). The 10% clarified egg yolk concentration gave the least post-thaw quality values and the greatest proportion of defective spermatozoa. This experiment found no advantage of replacing whole egg yolk with up to 15% clarified egg yolk in Mashona bull semen cryopreservation. However, 20% clarified and 20% whole egg yolk performed similarly in the maintenance of post-thaw sperm motility, viability and normal morphology.

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