Bio-electrosprays and Aerodynamically Assisted Bio-jets, Flow Cytometry Concepts for Interrogating Living Cells and Whole Organisms

2009 ◽  
Vol 1239 ◽  
Author(s):  
Suwan N. Jayasinghe
Keyword(s):  
Tissue Engineering ◽  
Flow Cytometry ◽  
Regenerative Medicine ◽  
Single Cell ◽  
Living Cells

AbstractBio-electrospraying and aerodynamically assisted bio-jetting are rapidly evolving approaches for directly handling living cells and organisms. In this article we demonstrate how these technologies now elucidated as being safe for handling living cells and organisms can be explored not only for tissue engineering and regenerative medicine but also in biology for single cell and organism diagnostics.

scholarly journals Applications of 3D Bioprinting in Tissue Engineering and Regenerative Medicine

2021 ◽  
Vol 10 (21) ◽  
pp. 4966
Author(s):  
Gia Saini ◽  
Nicole Segaran ◽  
Joseph L. Mayer ◽  
Aman Saini ◽  
Hassan Albadawi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Regenerative Medicine ◽  
Living Cells ◽  
Clinical Applications ◽  
3D Bioprinting ◽  
Future Directions ◽  
Specialized Tissue ◽  
Tissue Models ◽  
Functional Components

Regenerative medicine is an emerging field that centers on the restoration and regeneration of functional components of damaged tissue. Tissue engineering is an application of regenerative medicine and seeks to create functional tissue components and whole organs. Using 3D printing technologies, native tissue mimics can be created utilizing biomaterials and living cells. Recently, regenerative medicine has begun to employ 3D bioprinting methods to create highly specialized tissue models to improve upon conventional tissue engineering methods. Here, we review the use of 3D bioprinting in the advancement of tissue engineering by describing the process of 3D bioprinting and its advantages over other tissue engineering methods. Materials and techniques in bioprinting are also reviewed, in addition to future clinical applications, challenges, and future directions of the field.

scholarly journals Editorial for the Special Issue on 3D Printing for Tissue Engineering and Regenerative Medicine

Micromachines ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 366 ◽  
Author(s):  
Vahid Serpooshan ◽  
Murat Guvendiren
Keyword(s):  
Tissue Engineering ◽  
Additive Manufacturing ◽  
3D Printing ◽  
Regenerative Medicine ◽  
Three Dimensional ◽  
Living Cells ◽  
3D Bioprinting ◽  
Special Issue ◽  
3D Structures ◽  
Manufacturing Techniques

Three-dimensional (3D) bioprinting uses additive manufacturing techniques to fabricate 3D structures consisting of heterogenous selections of living cells, biomaterials, and active biomolecules [...]

DOZ047.67: Comparison of mesenchymal stem cells from pediatric normal and esophageal atresia patients for use in regenerative medicine

2019 ◽  
Vol 32 (Supplement_1) ◽  
Author(s):  
T Jensen ◽  
H Wanczyk ◽  
S Thaker ◽  
A Mitchell ◽  
I Sharma ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Flow Cytometry ◽  
Mesenchymal Stem Cells ◽  
Regenerative Medicine ◽  
Esophageal Atresia ◽  
Growth Kinetics ◽  
Population Doubling ◽  
Patient Specific ◽  
Stem Cell Markers

Abstract Esophageal atresia (EA) is a congenital defect in which the esophagus is not fully connected. Current treatments to bring together long gaps between the esophagus have very high complication rates and the replacement tissue does not possess the correct structure or physiologic properties. Tissue engineering and regenerative medicine have been suggested as a new patient-specific treatment for EA utilizing the patient's own cells and a tubular scaffold. Our group recently published on the successful regeneration of an esophagus using a retrievable scaffold seeded with adipose derived mesenchymal stem cells (AD-MSC) in a porcine model of esophageal loss. The goal of this study is to evaluate phenotypic differences between AD-MSCs from normal (N-AD-MSC) and EA patients (EA-AD-MSC) to determine if EA-AD-MSC could be used for autologous tissue engineering approaches. Subcutaneous fat was obtained from pediatric patients undergoing surgery (IRB#). The cells from all patients expressed mesenchymal stem cell markers CD90, CD105, CD73, CD44 and did not express hematopoietic markers CD45, CD34 and HLA-DR by flow cytometry. Growth kinetics revealed similar population doubling times and morphology was similar between the two groups. Some of the EA-AD-MSC demonstrated upregulated expression of ATNX1, BMI1, FOXF1, GLI2, GLI3, MYC, HOXC4 and NOG, known genotypic abnormalities in EA patients. None of the N-AD-MSCs had upregulated expression of these genes. Furthermore N-AD-MSCs and EA-AD-MSCs underwent osteogenic and adipogenic differentiation with positive staining for Oil Red O and Alizarian Red after 2 weeks in culture. The integrin profiles of these cells were also similar across all patients as demonstrated by flow cytometry. In conclusion, in vitro, the EA-AD-MSCs behaved similarly to N-AD-MSCs in growth kinetics, mesenchymal phenotype and differentiation ability. Genotypically there were some differences and how this will impact the growth of these cells on scaffolds is currently being investigated.

scholarly journals Bioinspired Networks of Communicating Synthetic Protocells

2021 ◽  
Vol 8 ◽  
Author(s):  
Patrick J. Grimes ◽  
Agostino Galanti ◽  
Pierangelo Gobbo
Keyword(s):  
Tissue Engineering ◽  
Synthetic Biology ◽  
Regenerative Medicine ◽  
Living Cells ◽  
Chemical Signals ◽  
Biological Cells ◽  
Bottom Up ◽  
Grand Challenges ◽  
The Past

The bottom-up synthesis of cell-like entities or protocells from inanimate molecules and materials is one of the grand challenges of our time. In the past decade, researchers in the emerging field of bottom-up synthetic biology have developed different protocell models and engineered them to mimic one or more abilities of biological cells, such as information transcription and translation, adhesion, and enzyme-mediated metabolism. Whilst thus far efforts have focused on increasing the biochemical complexity of individual protocells, an emerging challenge in bottom-up synthetic biology is the development of networks of communicating synthetic protocells. The possibility of engineering multi-protocellular systems capable of sending and receiving chemical signals to trigger individual or collective programmed cell-like behaviours or for communicating with living cells and tissues would lead to major scientific breakthroughs with important applications in biotechnology, tissue engineering and regenerative medicine. This mini-review will discuss this new, emerging area of bottom-up synthetic biology and will introduce three types of bioinspired networks of communicating synthetic protocells that have recently emerged.

Tissue engineering in regenerative medicine

2015 ◽  
Vol 6 (5) ◽  
pp. 291-298
Author(s):  
Barbara Różalska ◽  
Bartłomiej Micota ◽  
Małgorzata Paszkiewicz ◽  
Beata Sadowska
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine

In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts

2017 ◽  
Vol 68 (6) ◽  
pp. 1341-1344
Author(s):  
Grigore Berea ◽  
Gheorghe Gh. Balan ◽  
Vasile Sandru ◽  
Paul Dan Sirbu
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Endothelial Cells ◽  
Single Cell ◽  
Cell Line ◽  
Bone Repair ◽  
Umbilical Vein ◽  
Human Fibroblasts ◽  
Three Dimensional ◽  
Adipose Derived Stem Cells

Complex interactions between stem cells, vascular cells and fibroblasts represent the substrate of building microenvironment-embedded 3D structures that can be grafted or added to bone substitute scaffolds in tissue engineering or clinical bone repair. Human Adipose-derived Stem Cells (hASCs), human umbilical vein endothelial cells (HUVECs) and normal dermal human fibroblasts (NDHF) can be mixed together in three dimensional scaffold free constructs and their behaviour will emphasize their potential use as seeding points in bone tissue engineering. Various combinations of the aforementioned cell lines were compared to single cell line culture in terms of size, viability and cell proliferation. At 5 weeks, viability dropped for single cell line spheroids while addition of NDHF to hASC maintained the viability at the same level at 5 weeks Fibroblasts addition to the 3D construct of stem cells and endothelial cells improves viability and reduces proliferation as a marker of cell differentiation toward osteogenic line.

Graphene and Graphene-Based Materials in Biomedical Applications

2019 ◽  
Vol 26 (38) ◽  
pp. 6834-6850 ◽  
Author(s):  
Mohammad Omaish Ansari ◽  
Kalamegam Gauthaman ◽  
Abdurahman Essa ◽  
Sidi A. Bencherif ◽  
Adnan Memic
Keyword(s):  
Tissue Engineering ◽  
Thermal Properties ◽  
Gene Delivery ◽  
Regenerative Medicine ◽  
Biomedical Research ◽  
Biomedical Applications ◽  
Biomedical Application ◽  
Two Dimensional ◽  
Drug And Gene Delivery ◽  
Biomedical Fields

: Nanobiotechnology has huge potential in the field of regenerative medicine. One of the main drivers has been the development of novel nanomaterials. One developing class of materials is graphene and its derivatives recognized for their novel properties present on the nanoscale. In particular, graphene and graphene-based nanomaterials have been shown to have excellent electrical, mechanical, optical and thermal properties. Due to these unique properties coupled with the ability to tune their biocompatibility, these nanomaterials have been propelled for various applications. Most recently, these two-dimensional nanomaterials have been widely recognized for their utility in biomedical research. In this review, a brief overview of the strategies to synthesize graphene and its derivatives are discussed. Next, the biocompatibility profile of these nanomaterials as a precursor to their biomedical application is reviewed. Finally, recent applications of graphene-based nanomaterials in various biomedical fields including tissue engineering, drug and gene delivery, biosensing and bioimaging as well as other biorelated studies are highlighted.

2D, 3D and 4D Active Compound Delivery in Tissue Engineering and Regenerative Medicine

2015 ◽  
Vol 21 (12) ◽  
pp. 1506-1516 ◽  
Author(s):  
Nicolas Hanauer ◽  
Pierre Latreille ◽  
Shaker Alsharif ◽  
Xavier Banquy
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Active Compound
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