scholarly journals SHORT COMMUNICATION: THE EFFECT OF TANNIN SOLUTION IN PERFORMING SUCCESFULL EGG MICROINJECTION ON AFRICAN CATFISH (Clarias gariepinus)

2009 ◽  
Vol 4 (2) ◽  
pp. 153
Author(s):  
Muhammad Hunaina Fariduddin Ath-thar ◽  
Komar Sumantadinata ◽  
Alimuddin Alimuddin
Keyword(s):  
Short Communication ◽  
Clarias Gariepinus ◽  
Standard Operating Procedure ◽  
African Catfish ◽  
Hatching Rate ◽  
The Common ◽  
Transgenic Embryos ◽  
The Individual ◽  
Breeding And Genetics ◽  
Different Levels

In order to successfully perform a gene transfer activity using microinjection, an individual egg is needed. The common standard operating procedure to collect the individual egg is by applying chemical solution to remove the sticky layer covering the egg surface. Among several available solutions, tannin is one of alternative substances that can be used in performing egg microinjection on African catfish. In this study, the effect of different dosages of tannin solution to remove the sticky layer of eggs was observed. The study was conducted at the Laboratory of Fish Breeding and Genetics, Bogor Agricultural University. Prior to microinjection, the eggs were treated with different dosages of tannin solution right after fertilization. There were four different levels of dosage used as the treatments i.e.: 0.3, 0.5, 0.7, and 0.9 ppm. After the treatments, each individual egg was injected using DNA pmâactin-hrGFP plasmid with a concentration of 20 μg/mL. The results showed that the dosage of 0.5 ppm of tannin was the most effective treatment compared to the other dosages. This dosage produced 71.4% hatching rate and 57.1% transgenic embryos respectively.

scholarly journals Effect of Different Doses of Ovulin Hormone Suspended in Saline on the Induced Breeding Performance of African Catfishes Clarias anguillaris and Clarias gariepinus in Sokoto, Nigeria

2019 ◽  
pp. 1-8
Author(s):  
H. F. Maradun ◽  
L. A. Argungu ◽  
M. Y. Abubakar ◽  
M. Y. Abubakar ◽  
F. Umar ◽  
...  
Keyword(s):  
Significant Influence ◽  
Clarias Gariepinus ◽  
Fertilization Rate ◽  
African Catfish ◽  
Hatching Rate ◽  
Breeding Performance ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Different Levels ◽  
Different Doses

A study on the effect of different doses of Ovulin hormone suspended in saline on the breeding performance of Clarias anguillaris and Clarias gariepinus was carried out. The experiment was conducted in a 2x5 factorial experiment in a Completely Randomized Design at the Hatchery Unit of the Department of Fisheries and Aquaculture, Usmanu Danfodiyo University, Sokoto. Species and hormone dilutions constituted the factors with specie having 2 levels (C. anguillaris and C. gariepinus) and Ovulin suspended in saline at 5 levels (0%, 25%, 50%, 75% and 100%). The result showed that species levels did not significantly (P>0.05) affect the breeding performance in all the breeding performance parameters observed. However, fertilization rate, hatching rate and survival rate were significantly affected (P<0.05) by different levels of Ovulin suspended in saline, but did not have significant influence (P>0.05) on egg weight, spawning fecundity and relative fecundity. It could be concluded from this study that Ovulin suspended in saline can have significant influence on the breeding performance of African catfish.

scholarly journals Reproductive Performances and Egg Qualities in African Catfish (Clarias gariepinus) Broodstocks Supplemented with Curcumin and Thyroxine Hormone

Omni-Akuatika ◽  
2020 ◽  
Vol 16 (1) ◽  
pp. 32
Author(s):  
Livana Dethris Rawung ◽  
Damiana Rita Ekastuti ◽  
Muhammad Zairin Junior ◽  
Min Rahminiwati ◽  
Ade Sunarma ◽  
...  
Keyword(s):  
Reproductive Performance ◽  
Egg Quality ◽  
Gonadosomatic Index ◽  
Clarias Gariepinus ◽  
Density Lipoprotein ◽  
Cholesterol Concentration ◽  
Hepatosomatic Index ◽  
African Catfish ◽  
Hatching Rate ◽  
Significant Difference

An experiment was designed to study the effects of curcumin and thyroxine hormone supplementation in the diet to improve the reproductive performance of catfish broodstock by improving egg quality that eventually increases the production of seedlings. Catfish used in this experiment were supplemented with curcumin and thyroxine hormone through their feeds for 12 weeks. The results showed that there was no significant difference (p > 0.05) in the hepatosomatic index (HSI), gonadosomatic index (GSI), percentage of gonad maturity, total cholesterol concentration in the spawned eggs, high density lipoprotein (HDL) concentration in the spawned eggs, fertilization rate of spawned eggs, and hatching rate of fertilized eggs.  However, there were significant differences (p < 0.05) in the concentration of vitellogenin in the spawned eggs, egg diameter of the spawned eggs, and the triglycerides contents of spawned eggs. It was concluded that curcumin and thyroxine supplementations of African catfish increased vitellogenin concentrations and diameters of spawned eggs that have great potential to improve the reproductive performance.

scholarly journals Variasi Lama Kejutan Panas pada Suhu yang Sama terhadap Tingkat Penetasan Telur (HR) Ikan Lele Dumbo (Clarias gariepinus)

2020 ◽  
Vol 3 (1) ◽  
pp. 37
Author(s):  
Anny Rimalia
Keyword(s):  
Heat Shock ◽  
Clarias Gariepinus ◽  
Shock Duration ◽  
African Catfish ◽  
Hatching Rate ◽  
Egg Hatching ◽  
Temperature Shock ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Different Climates

<p><em>Species, temperature shock duration, fertilization temperature from different climates can cause different hatching results, so it is necessary to do research on the treatment of variations in heat shock at the same temperature, to obtain information about the hatching rate (HR) of African catfish (Clarias gariepinus). The method used is completely randomized design (3x3). The results of the egg hatching rate (HR), with variations in the heat shock at the same temperature (35⁰C), </em><em>showed the highest rate of hatching dumbo catfish eggs at heat shock one minute 233.00%, then heat shock two minutes 212.00%, and heat shock three minutes 200.00% respectively.</em><em></em></p>

scholarly journals Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus

2019 ◽  
Vol 21 (1) ◽  
Author(s):  
Hasan Nasrullah ◽  
Yanti Inneke Nababan ◽  
Ika Safitri ◽  
Dwi Yanti ◽  
Sri Nuryati ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Aeromonas Hydrophila ◽  
Short Communication ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Lysozyme Gene ◽  
Resistant Group ◽  
Susceptible Group

Abstract. Authors. 2020. Short Communication: Single nucleotide polymorphism in C-type lysozyme gene and its correlation with Aeromonas hydrophila resistance in African catfish Clarias gariepinus. Biodiversitas 21: 311-317. The chicken-type lysozyme (LYSC) gene has been demonstrated to play important roles in the fish protection system against bacterial infection. In this present study, we aimed to identify the single nucleotide polymorphism (SNP) within the LYSC gene of African catfish Clarias gariepinus and its possible association with Aeromonas hydrophila resistance, a major pathogenic bacterium in African catfish. The gDNA of the African catfish LYSC coding sequence was 1559 bp long, comprising of four exons and three introns. Six SNPs were identified in African catfish LYSC, namely SNP1-6. After the A. hydrophila challenge, we regarded the surviving individuals after the infection as the resistant group and the dead fish as the susceptible group. High-resolution melting (HRM) analysis on SNP2 revealed that the allele frequencies of TT, CC, and TC were of 27.78%, 5.55%, and 66.67% in the resistant group, while the frequencies of TT, CC, and TC were 16.67%, 27.78%, and 55.55% in the susceptible group, respectively. The expression of LYSC and other immune-genes in the resistant group was also higher in the liver, head kidney, and spleen. These results indicated that the LYSC gene might play an essential role in bacterial resistance, and the SNP2 within the LYSC gene may be associated with the resistance to A. hydrophila in African catfish.

scholarly journals Evaluation of Probiotic-Fermented Feed Addition and LaserFiring to Accelerate Mature Broodstocks and Seed Productions of African Catfish (Clarias gariepinus)

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
Pungky Slamet Wisnu Kusuma ◽  
Dyah Hariani ◽  
Akhmad Taufiq Mukti
Keyword(s):  
Clarias Gariepinus ◽  
Survival Rates ◽  
Fertilization Rate ◽  
Female Gonad ◽  
African Catfish ◽  
Hatching Rate ◽  
Male And Female ◽  
Gonadal Maturity ◽  
Fermented Feed ◽  
Laser Firing

This study aimed to determine the effects of fermented feed using probiotics and laser-firing to accelerate the mature broodstocks and seed productions of African catfish (Clarias gariepinus). Fish has used male and female broodstocks of catfish. The method was used a completely randomized design with three treatments: unfermented feed as control, probiotic-fermented feed (PFF), and probioticfermented feed+laser firing (PFF+Li). In the first study, a laser-firing dose of 1.125 Joule was performed on fish broodstocks every 15 days. The gonadal maturity time of male and female broodstocks was observed. The second study, treated female broodstocks, was mated with mature male broodstocks without any prior treatments (control). Fertilization rate, hatching rate, and seed production performances such as survival rate and total length were measured. The results showed that treatment of PFF+Li has a significant effect (P<0.05) on the gonadal maturity time of males and females. This treatment reaches the fastest time to mature of the female gonad (31-41 days) and the male gonad (32-37 days) than other treatments (P<0.05). This treatment also produced the highest fertilization, hatching, and survival rates of more than 90%, respectively, and the highest seed yield of 2.1-3.0 cm size compared to other treatments (P<0.05) in African catfish.

scholarly journals Effect of different water pH on hatching and survival rates of African catfish Clarias gariepinus (Pisces: Clariidae)

2019 ◽  
Vol 4 (2) ◽  
pp. 80-88
Author(s):  
Kasi Marimuthu ◽  
Harysooria Palaniandya ◽  
Zainal A. Muchlisin
Keyword(s):  
Water Quality ◽  
Incubation Period ◽  
Clarias Gariepinus ◽  
Survival Rates ◽  
Larval Survival ◽  
Low Ph ◽  
African Catfish ◽  
Hatching Rate ◽  
Water Ph ◽  
Ph Level

Water quality parameters influence the growth and survival of different stages of fishes and hence determining the optimal water quality variables is greatly important for any aquaculture farming. Among the variables, water pH is considered the key factors and plays an important role in the maintenance of the homeostasis in fishes. A study was conducted to determine the influence of different water pH on the incubation period, hatching rate and survival rate of African catfish (Clarias gariepinus) larvae. The fertilized eggs were incubated at 28.0 ± 1.0 °C with different levels of water pH ranging from 3-10. Twenty four pH levels were tested for incubation period and egg hatchability while 15 pH levels were tested for larval survivability in a completely randomized design with three replicates for each treatment. Just hatched larvae were used for this study and the effect was observed until 72 h post-hatching. Water pH was maintained by the addition of NaOH or H2SO4solutions. The incubation times of fertilized eggs were recorded to be 23.5–25.0 h at pH levels of 6.1–8.8. The increased incubation time was noticed at the rest of the acidic and alkaline pH levels. Significantly highest hatching rate was observed at the pH levels of 6.7–7.6 compared to those at lower and higher pH levels (P0.05). However, no significant differences (P0.05) were recognized in the hatching rates at the pH levels of 6.7 – 7.6. No hatching occurred at low pH levels between 3.1 and 3.4 and at high pH of 10. After 72 h of exposure to different pH levels, no larvae survived at pH levels below 4.5 and above 9.0.  Highest larval survival (98%) was observed at pH 7.0 followed by 94% at pH 7.5 and 92% at pH 6.5. The results obtained in the present study revealed embryos and larvae can survive and tolerate to a low pH level of 3.7 and 4.5, respectively but the survival rates decreased with decreasing pH levels. Therefore, a water pH level of 6.7–7.5 is highly recommended for optimal hatching and highest larval viability of C. gariepinus.

scholarly journals Artificial propagation and larval breeding tests of the african sharptooth catfish, Clarias gariepinus conducted in captive conditions

2021 ◽  
Vol 4 (1) ◽  
Keyword(s):  
Clarias Gariepinus ◽  
Survival Rates ◽  
Wire Mesh ◽  
Individual Growth ◽  
Natural Food ◽  
African Catfish ◽  
Hatching Rate ◽  
Water Lettuce ◽  
Artificial Food ◽  
Artificial Propagation

The African catfish Clarias gariepinus is the second most cultured species in many African countries, including Senegal. Despite its economic importance in local aquaculture, artificial propagation of the species that would allow obtaining quality seed throughout the year has not yet been mastered adequately. This study aimed at producing C. gariepinus quality and mass supply seed to enhance local production. Three different types of incubation (Macdonald's bottle, water lettuce and wire mesh frame) were tested to identify the most efficient and accessible method for the producers at low cost and with a high hatching rate. The hatching rates obtained for the three different incubation methods were 26.9%, 10.0% and 6.3% for the Macdonald bottle (MB), wire frame (WF) and water lettuce (WL), respectively, while the corresponding survival rates were 91.6%, 70.4% and 77.8%.77.8%. The high hatching rate obtained with the MB could be due to the influence of permanent water circulation, which is absent in the other two hatching systems. The survival rates decreased 15 days after hatching and were 3.24%, 2.04% and 7.16%, respectively. The survival rates obtained three days after hatching were satisfactory, but the significant decrease at 15 days after hatching especially during the post- weaning phase seems to be due to the food quality, and unrelated to the hatching system. The daily individual growth, weight gain and body size of the larvae was significantly higher for larvae fed with natural food compared those that received artificial food. These results indicate that larvae preferred and fed the natural food which was permanently available in the rearing tanks. The artificial food deposited on the bottom of the tank changes appearance over time and may not be appreciated and efficiently consumed by the larvae, which may negatively impact their growth performance.

scholarly journals 6-Desaturase-Like Encoding Gene Introduction in Catfish (Clarias gariepinus)

Omni-Akuatika ◽  
2017 ◽  
Vol 13 (2) ◽  
Author(s):  
Anny Hary Ayu ◽  
Alimuddin Alimuddin ◽  
Dedi Jusadi
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Biosynthesis ◽  
Clarias Gariepinus ◽  
Pulse Length ◽  
Transgenic Fish ◽  
Pulse Interval ◽  
African Catfish ◽  
Hatching Rate ◽  
Sperm Viability ◽  
Δ6 Desaturase

African catfish (Clarias gariepinus) is one of the economically valuable aquaculture fish species in Indonesia. This research was aimed to produce F0 transgenic catfish carrying masou salmon Δ6-desaturase-like (OmΔ6FAD) gene. The Δ6-desaturase enzyme is involved in highly unsaturated fatty acid biosynthesis. Transgenic catfish was produced by sperm-mediated gene transfer using electroporation method. In this study, as the first step, sperms were electroporated with three different OmΔ6FAD concentration (25, 50, and 100 µg mL-1) to have the highest sperm viability after electroporation (125 V/cm, pulse frequency 5 times, pulse length 30 millisecond, pulse interval 0.1 second). The highest sperm viability and sperm carrying OmΔ6FAD were obtain at 100 µg mL-1. This concentration was then used to produce F0 transgenic catfish in the second step. Sperm motility, sperm viability, fertilization rate, hatching rate, and larval survival at 14 days after hatching were the same as the controls (p>0.05). Genomic DNA was extracted from caudal fin and then used as template to identify transgenic F0 by PCR method using specific primer for OmΔ6FAD gene. The PCR result showed that 53.84% of F0 carried OmΔ6FAD gene. The result of fatty acid analysis showed that EPA and DHA contents of F0 transgenic fish and non-transgenic fish were similar. Keywords: catfish, Δ6-desaturase-like gene, fatty acids, electroporation

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