scholarly journals PERBANYAKAN TANAMAN HIAS AIR Microsorum pteropus MELALUI KULTUR KANTONG SPORA PADA BERBAGAI SUBSTRAT

2019 ◽  
Vol 14 (4) ◽  
pp. 253
Author(s):  
Muhamad Yamin ◽  
Tutik Kadarini ◽  
Lili Solichah
Keyword(s):  
Rice Husk ◽  
Rice Husk Ash ◽  
Culture Media ◽  
Transparent Plastic ◽  
Wood Shavings ◽  
Novel Approach ◽  
Soil Media ◽  
Propagation Media ◽  
Young Sporophytes

Produksi massal tanaman hias air pakis jawa Microsorum pteropus melalui pemotongan rhizoma terlihat kurang efisien sedangkan melalui kultur in vitro spora masih sulit dilakukan masyarakat. Salah satu pendekatan baru dalam perbanyakan tanaman M. Pteropus melalui kultur kantong spora telah berhasil dilakukan. Tujuan penelitian adalah untuk menentukan media dalam perbanyakan tanaman M. pteropus melalui kultur kantong spora. Potongan daun yang mengandung satu kantong spora diletakkan di atas media tanam, ditutup dengan plastik transparan, dan ditaruh pada lingkungan di luar ruangan (outdoor). Media tanam yang digunakan yaitu: A) cacahan akar pakis; B) serutan kayu; C) cacahan akar pakis + serutan kayu; D) cacahan akar pakis + serutan kayu + kompos; E) pasir gunung berapi (pasir malang); F) abu sekam padi; G) pasir; dan H) tanah. Hasil penelitian menunjukkan bahwa perkembangan sporofit muda (young sporophye) sudah mulai terlihat pada bulan pertama sampai ketiga setelah tanam. Sporofit muda yang berkembang dari kantong spora yang dipelihara pada media pasir, media tanah dan media campuran serutan kayu + akar pakis + kompos menunjukkan rata-rata persentase tanaman hidup yang paling tinggi yaitu 48%. Sebaliknya sporofit muda paling sedikit berkembang pada media akar pakis, media abu sekam padi dan media campuran akar pakis, dan serutan kayu. Berdasarkan hasil tersebut maka media terbaik untuk perbanyakan M. pteropus di luar ruangan melalui kultur kantong spora adalah media tanah dan media campuran akar pakis + serutan kayu + kompos.Mass production of Microsorum pteropus through rhizome cuttings has been deemed not efficient while the application of in vitro culture of its spores is still technically difficult to be performed by farmers. A novel approach to mass-produce M. pteropus trough sori culture has been developed and is relatively easy to perform. This study was aimed to determine a suitable propagation media for sori culture of M. pteropus. Small cut fronds containing one sorus were laid on the culture media and covered with a transparent plastic sheet and left on outdoor conditions. The culture media used were: A) fern-root; B) wood shavings; C) fern-root + wood shavings; D) fern-root + wood shavings + compost; E) volcanic sand; F) rice husk ash; G) sand; and H) soil. The results showed that young sporophytes developed in the 1st to 3rd month after culture. The young sporophytes developed in the sand, soil and mixture of wood shavings + fern-root + compost medium showed higher numbers of live plants (48%). In contrast, the lower numbers of live young sporophyte were found in the fern-root, rice husk ash, and mixture of fern-root + wood shavings medium. Based on these results, the best alternative media for propagation of M. pteropus through sori culture on the outdoor conditions are soil media and the mixture of fern roots + wood shavings + compost media.

scholarly journals Pertumbuhan miselia jamur merang (Volvariella volvaceae) lokasi pacing dengan jenis media dan konsentrasi biakan murni secara in vitro

Jurnal Agro ◽  
10.15575/2426 ◽  
2018 ◽  
Vol 5 (2) ◽  
pp. 104-126
Author(s):  
Ani Lestari ◽  
Elia Azizah ◽  
Kuswarini Sulandjari ◽  
Abdulloh Yasin
Keyword(s):  
Rice Husk ◽  
Pure Culture ◽  
Culture Media ◽  
Block Design ◽  
Rice Husks ◽  
Colony Diameter ◽  
Randomized Block Design ◽  
Experiment Method ◽  
Straw Mushroom

Jamur merang merupakan salah satu jamur konsumsi yang sangat diminati, sehingga kebutuhan jamur merang semakin meningkat. Namun untuk memenuhi kebutuhan tersebut banyak ditemukan kendala, salah satunya dalam penyediaan biakan murni terkait jenis dan konsentrasi media biakan murni. Penelitian ini bertujuan untuk mendapatkan jenis dan konsentrasi media biakan murni yang memberikan pertumbuhan koloni miselia jamur merang tertinggi. Penelitian dilaksanakan di Laboratorium Bioteknologi Tanaman Fakultas Pertanian UNSIKA dari Maret sampai Agustus 2017, menggunakan rancangan acak kelompok faktorial dengan 3 ulangan. Faktor pertama : jenis media dengan 4 taraf  m1 = PDA, m2 = arang sekam padi, m3 = jerami, m4 = campuran arang sekam padi dan jerami. Faktor kedua : konsentrasi media biakan murni dengan 3 taraf : k1 = 200 g l-1, k2 = 250 g l-1, k3 = 300 g l-1. Hasil penelitian ini menunjukkan bahwa, media arang sekam padi dan PDA dengan konsentrasi media biakan murni 200 g l-1 memberikan pengaruh yang sama baiknya bagi pertumbuhan miselium jamur merang dengan diameter sebesar 8 cm.  Media arang sekam padi dapat dijadikan sebagai alternatif pengganti media PDA. Straw mushroom is one of the most popular mushroom. So needs of straw mushroom more  increasing. However, to meet those needs had been founded obstscles, one of them is type and consentration of straw mushroom pure culture media. The aims of this research was to find out the type and consentration of pure culture media which could give the best growth of straw mushroom mycelia. The research was conducted in laboratory of plant bioctehnology, faculty of Agriculture, University of Singaperbangsa Karawang from March until August 2017. The experiment method used the factorial randomized block design with 3 replications. The first factor was the type of media, consisted of 4 levels m1 = Potato dextrose agar (PDA), m2 = rice husks m3= Rice Straw m4 = Mixture of rice husk and straw. The second factor was the concentration of media, consisted of 3 levels k1 = 200 g l-1, k2 = 250 g l-1, k3 = 300 g l-1. The results of the research showed that rice husk media and PDA with consentration’s 200 g l-1 gave good affect to mycelia colony diameter of straw mushroom by 8 cm. The rice huskwas an alternative as pure culture media for straw mushroom, substitute PDA.

scholarly journals An Intracellular Tripeptide Arg-His-Trp of Serum Origin Detected in MCF-7 Cells Is A Possible Agonist to β2 Adrenoceptor

2021 ◽  
Vol 28 ◽  
Author(s):  
Hritik Chandore ◽  
Ajay Kumar Raj ◽  
Kiran Bharat Lokhande ◽  
K. Venkateswara Swamy ◽  
Jayanta K. Pal ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Culture Media ◽  
Positive Control ◽  
Vertical Tube ◽  
Chronic Obstructive ◽  
Obstructive Pulmonary Disease ◽  
Intracellular Metabolites ◽  
Novel Approach ◽  
Mcf 7

Background: The need of agonists and antagonists of β2 adrenoceptor (β2AR) is warranted in various human disease conditions including cancer, cardiovascular and other metabolic disorders. However, the sources of agonists of β2AR are diverse in nature. Interestingly, there is a complete gap in the exploration of agonists of β2AR from serum that is a well-known component of culture media which supports growth and proliferation of normal and cancer cells in vitro. Methods: In this paper, we employed a novel vertical tube gel electrophoresis (VTGE)-assisted purification of intracellular metabolites of MCF-7 cells grown in vitro in complete media with fetal bovine serum (FBS). Intracellular metabolites of MCF-7 cells were then analyzed by LC-HRMS. Identified intracellular tripeptides of FBS origin were evaluated for their molecular interactions with various extracellular and intracellular receptors including β2AR (PDB ID: 2RH1) by employing molecular docking and molecular dynamics simulations (MDS). A known agonist of β2AR, isoproterenol was used as a positive control in molecular docking and MDS analyses. Results : We report here identification of a few novel intracellular tripeptides, namely Arg-His-Trp, (PubChem CID-145453842), Pro-Ile-Glu, (PubChem CID-145457492), Cys-Gln-Gln, (PubChem CID-71471965), Glu-Glu-Lys, (PubChem CID-11441068) and Gly-Cys-Leu (PubChem CID-145455600) of FBS origin in MCF-7 cells. Molecular docking and MDS analyses revealed that among these molecules, the tripeptide Arg-His-Trp shows a favorable binding affinity with β2AR (-9.8 Kcal/mol). The agonistic effect of Arg-His-Trp is significant and comparable with that of a known agonist of β2AR, isoproterenol. Conclusion: In conclusion, we identified a unique Arg-His-Trp tripeptide of FBS origin in MCF-7 cells by employing a novel approach. This unique tripeptide Arg-His-Trp is suggested to be a potential agonist of β2AR and it may have applications in the context of various human diseases like bronchial asthma and chronic obstructive pulmonary disease (COPD).

In vitro cytocompatibility testing of oxidative degradation products

2021 ◽  
pp. 088391152110031
Author(s):  
Scott M Herting ◽  
Mary Beth B Monroe ◽  
Andrew C Weems ◽  
Sam T Briggs ◽  
Grace K Fletcher ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Culture ◽  
Culture Media ◽  
Degradation Products ◽  
Oxidative Degradation ◽  
Cell Culture Media ◽  
Novel Approach ◽  
Gene And Protein Expression

Implantable medical devices must undergo thorough evaluation to ensure safety and efficacy before use in humans. If a device is designed to degrade, it is critical to understand the rate of degradation and the degradation products that will be released. Oxidative degradation is typically modeled in vitro by immersing materials or devices in hydrogen peroxide, which can limit further analysis of degradation products in many cases. Here we demonstrate a novel approach for testing the cytocompatibility of degradation products for oxidatively-degradable biomaterials where the materials are exposed to hydrogen peroxide, and then catalase enzyme is used to convert the hydrogen peroxide to water and oxygen so that the resulting aqueous solution can be added to cell culture media. To validate our results, expected degradation products are also synthesized then added to cell culture media. We used these methods to evaluate the cytocompatibility of degradation products from an oxidatively-degradable shape memory polyurethane designed in our lab and found that the degradation of these polymers is unlikely to cause a cytotoxic response in vivo based on the guidance provided by ISO 10993-5. These methods may also be applicable to other biocompatibility tests such as tests for mutagenicity or systemic toxicity, and evaluations of cell proliferation, migration, or gene and protein expression.

scholarly journals Tunable Extracellular Self-Assembly of Multi-Protein Conjugates from Bacillus subtilis

2016 ◽  
Author(s):  
Charlie Gilbert ◽  
Mark Howarth ◽  
Colin R. Harwood ◽  
Tom Ellis
Keyword(s):  
Bacillus Subtilis ◽  
Self Assembly ◽  
Culture Media ◽  
Protein Complexes ◽  
Modular System ◽  
Protein Conjugation ◽  
Novel Approach ◽  
Multifunctional Enzymes

The ability to stably and specifically conjugate recombinant proteins to one another is a powerful in vitro technique for engineering multifunctional enzymes, protein therapeutics and novel biological materials. However, for many applications spontaneous in vivo protein conjugation would be preferable to in vitro methods. Exploiting the recently described SpyTag-SpyCatcher system, we describe here how enzymes and structural proteins can be genetically-encoded to covalently conjugate in culture media following programmable secretion by Bacillus subtilis. Using this novel approach, we demonstrate how self-conjugation of a secreted industrial enzyme, XynA, dramatically increases its resilience to boiling and we show that cellular consortia can be engineered to self-assemble functional multi-protein complexes with tunable composition. This genetically-encoded modular system provides a new, flexible strategy for protein conjugation harnessing the substantial advantages of extracellular self-assembly.

scholarly journals Direct conversion of human fibroblast to hepatocytes using a single inducible polycistronic vector

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Maria Ballester ◽  
Miguel Bolonio ◽  
Ramon Santamaria ◽  
José V. Castell ◽  
Carmen Ribes-Koninckx ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Lentiviral Vector ◽  
Culture Media ◽  
Human Fibroblasts ◽  
Direct Conversion ◽  
Metabolic Functions ◽  
Novel Approach ◽  
Different Genetic Background ◽  
Green Fluorescent

Abstract Background Human fibroblasts can be reprogrammed into induced hepatocyte-like cells through the expression of a set of transcription factors. Although the generation of induced hepatocyte-like cells by HNF4A, HNF1A, and FOXA3 expression has proven to be a robust experimental strategy, using multiple lentivirus results in a highly variable heterogeneous population. Methods We designed and implemented a novel approach based on the delivery of reprogramming factors and green fluorescent protein in a single doxycycline-inducible lentiviral vector using 2A self-cleaving peptides. Results Fibroblasts infected with the lentiviral vector can be amplified in basic fibroblast culture media in the absence of doxycycline without induction of hepatic genes. Upon switching to hepatic maturation media containing doxycycline, cells stop proliferating, activate hepatic gene transcription, and perform metabolic functions characteristic of hepatocytes. Conclusion Our strategy can generate an unlimited source of homogeneously induced hepatocyte-like cells from different genetic background donors, capable of performing typical hepatic functions suitable for drug research and other in vitro applications.

Development of white brick fuel cell using rice husk ash agricultural waste for sustainable power generation: A novel approach

2021 ◽  
Author(s):  
Verjesh Kumar Magotra ◽  
S.J. Lee ◽  
Akbar I. Inamdar ◽  
T.W. Kang ◽  
Pundalik D. Walke ◽  
...  
Keyword(s):  
Power Generation ◽  
Fuel Cell ◽  
Rice Husk ◽  
Rice Husk Ash ◽  
Agricultural Waste ◽  
Novel Approach ◽  
Sustainable Power

Bioactive Porous Silica Ceramics Prepared Using Rice Husk Ash by Gelcasting Method

2012 ◽  
Vol 548 ◽  
pp. 12-16 ◽  
Author(s):  
Jyoti Prakash Nayak ◽  
Japes Bera
Keyword(s):  
Rice Husk ◽  
Rice Husk Ash ◽  
Porous Silica ◽  
Silica Xerogel ◽  
Silica Body ◽  
In Vitro Bioactivity ◽  
Casting Method ◽  
Gel Casting ◽  
Cross Linker

Silica-based porous bioactive ceramics was prepared by gel-casting method using silica xerogel powder. Xerogel was derived from rice husk ash. 42 vol.% solid containing slurry was prepared in 1:30 (MBAM:AM) monomer cross-linker solution. The srurry was thixotropic. Gel-casted body was machined efficiently. Dired cast body was sintered at 1100oC. Apatite layer was formed on silica body during In vitro bioactivity experiment. The results suggest that the gel-casted silica ceramics can be used as a bioceramics.

Structural integrity after cold storage of human epidermal model in hypothermosol: A correlative ultrastructural and fluorescent assay

1994 ◽  
Vol 52 ◽  
pp. 270-271
Author(s):  
Henry H. Eichelberger ◽  
John G. Baust ◽  
Robert G. Van Buskirk
Keyword(s):  
Cold Storage ◽  
Structural Integrity ◽  
Culture Media ◽  
Cell Structure ◽  
Natural State ◽  
Sodium Cacodylate ◽  
Ruthenium Tetroxide ◽  
Cacodylate Buffer ◽  
Before And After

For research in cell differentiation and in vitro toxicology it is essential to provide a natural state of cell structure as a benchmark for interpreting results. Hypothermosol (Cryomedical Sciences, Rockville, MD) has proven useful in insuring the viability of synthetic human epidermis during cold-storage and in maintaining the epidermis’ ability to continue to differentiate following warming.Human epidermal equivalent, EpiDerm (MatTek Corporation, Ashland, MA) consisting of fully differentiated stratified human epidermal cells were grown on a microporous membrane. EpiDerm samples were fixed before and after cold-storage (4°C) for 5 days in Hypothermosol or skin culture media (MatTek Corporation) and allowed to recover for 7 days at 37°C. EpiDerm samples were fixed 1 hour in 2.5% glutaraldehyde in sodium cacodylate buffer (pH 7.2). A secondary fixation with 0.2% ruthenium tetroxide (Polysciences, Inc., Warrington, PA) in sodium cacodylate was carried out for 3 hours at 4°C. Other samples were similarly fixed, but with 1% Osmium tetroxide in place of ruthenium tetroxide. Samples were dehydrated through a graded acetone series, infiltrated with Spurrs resin (Polysciences Inc.) and polymerized at 70°C.

Mechanoactivation Of Rice Husk Ash In Laboratory Conditions

2019 ◽  
pp. 20-26
Keyword(s):  
Mechanical Activation ◽  
Rice Husk ◽  
Amorphous Silica ◽  
Silicon Oxide ◽  
Rice Husk Ash ◽  
Pozzolanic Activity ◽  
Maximum Solubility ◽  
Laboratory Conditions ◽  
Before And After ◽  
Mineral Additive

In many rice producing countries of the world, including in Vietnam, various research aimed at using rice husk ash (RHA) as a finely dispersed active mineral additive in cements, concrete and mortars are being conducted. The effect of the duration of the mechanoactivation of the RHA, produced under laboratory conditions in Vietnam, on its pozzolanic activity were investigated in this study. The composition of ash was investigated by laser granulometry and the values of indicators characterizing the dispersion of its particles before and after mechanical activation were established. The content of soluble amorphous silicon oxide in rice husk ash samples was determined by photocolorimetric analysis. The pizzolanic activity of the RHA, fly ash and the silica fume was also compared according to the method of absorption of the solution of the active mineral additive. It is established that the duration of the mechanical activation of rice husk ash by grinding in a vibratory mill is optimal for increasing its pozzolanic activity, since it simultaneously results in the production of the most dispersed ash particles with the highest specific surface area and maximum solubility of the amorphous silica contained in it. Longer grinding does not lead to further reduction in the size of ash particles, which can be explained by their aggregation, and also reduces the solubility of amorphous silica in an aqueous alkaline medium.

Desalination Technology of Water by using Rice Husk Ash

2014 ◽  
Vol 27 (2) ◽  
pp. 148-160
Author(s):  
Hassan K. Hassan ◽  
Najla J. Al-Amiri ◽  
Mohammed M. Yassen
Keyword(s):  
Rice Husk ◽  
Rice Husk Ash ◽  
Desalination Technology
Sign in / Sign up

Export Citation Format

Share Document