scholarly journals EVALUASI PERFORMA BIBIT RUMPUT LAUT Gracilaria verrucosa HASIL KULTUR JARINGAN DI KABUPATEN LUWU, SULAWESI SELATAN

2018 ◽  
Vol 13 (1) ◽  
pp. 1
Author(s):  
Makmur Makmur ◽  
Sri Redjeki Hesti Mulyaningrum

Penelitian ini bertujuan untuk mengevaluasi performa bibit rumput laut Gracilaria verrucosa. hasil kultur jaringan yang dibudidayakan di salah satu daerah sentra produksi rumput laut di Sulawesi Selatan. Penelitian dilaksanakan di Desa Murante Kecamatan Suli Kabupten Luwu, Sulawesi Selatan pada 2014 selama tiga siklus pemeliharaan dengan lama pemeliharaan 40 hari/siklus. Luasan tambak yang digunakan pada siklus pertama adalah 1,2 ha; siklus kedua 2 ha; dan siklus ketiga 4 ha. Sebagai perlakuan adalah bibit rumput laut hasil kultur jaringan dibandingkan dengan rumput laut lokal sebagai kontrol. Padat penebaran bibit rumput laut adalah 1.000 kg/ha yang dipelihara dengan metode tebar dasar. Penimbangan rumput laut dilakukan pada awal dan akhir penelitian untuk mengetahui laju pertumbuhan harian (LPH) dan produksi, sedangkan kandungan agar dianalisis setelah panen. Analisis data dilakukan secara deskriptif dan disajikan dalam bentuk grafik dan tabel. Dari tiga siklus budidaya menunjukkan bahwa bibit rumput laut hasil kultur jaringan memiliki performa pertumbuhan, produksi, dan kandungan agar yang lebih tinggi dibandingkan bibit rumput laut lokal dengan perbedaan masing-masing 12,1%; 17,8%; dan 6,7%. Pertumbuhan tertinggi terjadi pada siklus kedua, sedangkan kandungan agar tertinggi terjadi pada siklus ketiga baik pada bibit hasil kultur jaringan maupun bibit lokal. Bibit rumput laut Gracilaria sp. hasil kultur jaringan dapat dijadikan alternatif sumber bibit yang berkualitas.This study was aimed to evaluate the performance of G. verrucosa seaweed seed produced from tissue culture and cultivated at the seaweed production center in Murante Village Suli District Luwu Regency South Sulawesi in 2014. The seeds were cultivated for three production cycles, with each cycle lasted for 40 days. The first culture period was conducted in 1.2 ha, the second in 2 ha, and the third in 4 ha of pond areas. The treatments consisted of two different seaweed seed sources, i.e: tissue cultured seaweed seed and local seaweed as a control. The seaweed stocking density used was 1.000 kg/ha cultivated using broadcast method. The daily growth rate (DGR) and biomass as production indicators were measured at the initial and the end of the culture period. The agar yield was measured after harvest. The data were analyzed descriptively and presented in graphical visualization and data tabulation. The present study showed that tissue cultured seaweed had a higher performance in terms of growth, biomass production, and agar yield with the values of 12.1%, 17.8%, and 6.7%, respectively, than that of the local seedling. The highest DGR was produced at the second cycle, and the highest agar yield was measured at the third cycle for both of seaweed seedlings. Given this result, tissue cultured seaweed can be an alternative source of quality seeds.

2019 ◽  
Vol 14 (3) ◽  
pp. 153
Author(s):  
Sri Redjeki Hesti Mulyaningrum ◽  
Andi Indra Jaya Asaad ◽  
Hidayat Suryanto Suwoyo ◽  
Erfan Andi Hendrajat

Penelitian ini bertujuan untuk menguji performa bibit rumput laut Gracilaria verrucosa hasil kultur jaringan yang diremajakan dengan metode seleksi massa. Penelitian dilaksanakan di tambak percobaan Marana, Maros, Sulawesi Selatan; menggunakan dua petakan tambak berukuran sekitar 2.500 m2. Seleksi dilakukan berdasarkan perhitungan laju pertumbuhan harian (LPH), menggunakan sistem long line selama dua siklus dengan durasi 30 hari/siklus. Bibit rumput laut hasil seleksi kemudian dipelihara dengan sistem tebar dasar dan dibandingkan dengan kontrol internal dan kontrol eksternal dengan memasang sembilan unit hapa berukuran 1 m x 1 m x 1 m, dengan tiga ulangan untuk masing-masing perlakuan. Perhitungan LPH, kandungan agar, dan kekuatan gel dilakukan setiap 30 hari dan setiap 15 hari dilakukan monitoring terhadap kualitas air yakni salinitas, suhu, nitrat, dan fosfat. Hasil yang diperoleh memperlihatkan adanya peningkatan LPH yang signifikan (P<0,05) dari siklus-I (2,67 ± 0,38%/hari) menjadi (3,56 ± 0,25%/hari) pada siklus-II, dengan respons seleksi sebesar 21,48%-22,34% dan diferensial seleksi sebesar 9,70%-10,16%. Pertumbuhan rumput laut hasil seleksi lebih tinggi (3,23 ± 0,50%/hari) dibandingkan dengan kontrol internal (2,12 ± 0,02%/hari), dan kontrol eksternal (1,69 ± 0,09%/hari) (P<0,05). Kontrol internal memiliki kandungan agar yang lebih tinggi (20,07 ± 12,97%) dari rumput laut hasil seleksi (16,07 ± 1,58%) dan kontrol eksternal (8,29 ± 1,69%) (P<0,05); namun hasil seleksi memiliki kekuatan gel yang lebih tinggi (761,15 ± 208,90 g/cm2) dari kontrol internal (322,44 ± 244,29 g/cm2), dan kontrol eksternal (297,42 ± 44,16 g/cm²) (P<0,05). Peremajaan bibit rumput laut hasil kultur jaringan dengan metode seleksi massa mampu meningkatkan performa bibit rumput laut G. verrucosa.This study was aimed to determine the performance of tissue-cultured seaweed seed Gracilaria verrucosa, which was propagated using mass selection method. The study was conducted in the experimental ponds in Marana, Maros,  South Sulawesi. The mass selection was conducted in two ponds of 2,500 m2. The selection was made by culturing the seaweed seed using the long line system for two cycles in 30 days/cycle duration during which the daily growth rates (DGR) of seaweed seeds were measured. The selected seaweed seeds were then cultivated in the ponds using broadcast system and the growth was compared with the internal and external controls consisted of nine units of 1 m x 1 m x 1 m hapa, with three replications for each treatment (selected seed, internal control, and external control). The measurements of DGR, agar yield, and gel strength were conducted every 30 days, while water quality monitoring i.e. salinity, temperature, nitrate, and phosphate were conducted every 15 days. The results showed that the DGR of seaweed seed increased significantly (P<0.05) from 2.67 ± 0.38%/day in cycle-I to 3.56 ± 0.25%/day in cycle-II, the selection response was 21.48%-22.34% and the differential selection was 9.70%-10.16%. The daily growth rate of selected seaweed seed was significantly higher (3.23 ± 0.50%/day) (P<0.05) compared to internal (2.12 ± 0.02%/day) and external controls (1.69 ± 0.09%/day). Internal control has higher agar yield (20.07 ± 12.97%) compared to the selected seed (16.07 ± 1.58%) and external control (8.29 ± 1.69%) (P<0,05). The selected seed has higher gel strength (761.15 ± 208.90 g/cm2) compared to internal control (322.44 ± 244.29 g/cm2), and external control (297.42 ± 44.16 g/cm2) (P<0,05). Propagated tissue-cultured seaweed seed using the  mass selection method could improve the overall performance of seaweed seed. 


2018 ◽  
Vol 23 (1) ◽  
pp. 55
Author(s):  
Sri Redjeki Hesti Mulyaningrum ◽  
Hidayat Suryanto Suwoyo

Mass propagation of tissue culture produced seaweed seedling was conducted in the pond. The present study aims to evaluate the growth of tissue culture produced seaweed seedling of Gracilaria verrucosa, its agar yield and related water quality variables during propagation in pond. The seedling propagation was conducted in pond using long line method for 9 cycles (30 days per cycle). The daily growth rate was calculated by weighing 30% of the total amount of seaweed at ropes line every 15 days. Agar yield was analyzed every 30 days in hot water extraction method. Water quality monitoring was conducted every 15 days. The data of daily growth rate and agar yield were analyzed descriptively. A simple linear regression analysis was conducted in order to analyze the relationship between growth and agar yield as well relationship between water quality variables, growth and agar yield. The result showed that the average daily growth rate of nine cycles was 3.38±1.49%. The highest agar yield was yielded from cycle II (27.84±1.60%) and the lowest was in cycle I (10.30%±2.15). There is no relationship between daily growth rate and agar yield (R2=0.055). Daily growth rate was mostly influenced by phosphate (P<0.05), nitrate (P<0.05), salinity (P<0.05) and light penetration (P<0.05). Meanwhile, agar yield was mostly influenced by temperature (P<0.05) and light penetration (P<0.05). Present study promising for seaweed G. verrucosa mass cultivation of tissue culture product which is a feasible alternative method to supply seaweed seedlings in seaweed farming development.  Keywords: growth, agar yield, G. verrucosa, tissue culture, water quality


2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Darwis Darwis ◽  
Joppy D. Mudeng ◽  
Sammy N.J. Londong

This research aimed to determine the stocking density that support the best growth and survival rate of carp cultivated in aquaponic systems, and to study the water quality of carp culture with different stocking densities in aquaponic systems. The study used 12 aquaria measuring 40x40x40 cm each (water volume of 50 liters). The experimental fish are carp (Cyprinus carpio) weighing in average of 3.5 g/individual.  The fish were cultivated with different stocking densities as treatment, including A = 4; B = 7; C = 10 and D = 13 individuals/aquarium.  The fish were fed with pellet at 5%/body weight/day with a feeding frequency of two times a day. The study was conducted for 4 weeks. Data consisting of daily growth, survival and water quality parameters (temperature, pH, dissolved oxygen, ammonia, nitrite and nitrate) were measured once a week. The data obtained were analyzed by ANOVA. The results of the study showed that the difference in stocking density had no significant effect on the growth and survival of carp.  Water quality was in a reasonable range to support the growth and survival of carp cultivated with aquaponic systems.Keywords: cultivation, carp, aquaponics, stocking density, growth


2017 ◽  
Vol 12 (1) ◽  
pp. 19 ◽  
Author(s):  
Rachman Syah ◽  
Makmur Makmur ◽  
Mat Fahrur

Upaya meningkatkan produktivitas lahan tambak dapat dilakukan dengan meningkatkan padat penebaran disertai dengan pemberian akuinput yang prima serta dukungan teknologi yang memadai. Tiga padat penebaran yaitu 750; 1.000; dan 1.250 ekor/m2, diaplikasikan pada tambak dengan luasan 1.000 m2 dengan kedalaman air 1,8 m dilengkapi dengan sistem aerasi berupa kincir dan root blower, pompa submersible, automatic feeder, central drain dan collector drain serta Instalasi Pengolah Air Limbah (IPAL). Kapasitas sistem aerasi adalah 500 kg biomassa udang/HP. Udang dipelihara selama 105 hari. Hasil penelitian menunjukkan bahwa padat penebaran yang diaplikasikan menghasilkan bobot akhir udang yang relatif sama berkisar 15,48-16,30 (15,78±0,45) g/ekor dengan nilai pertumbuhan harian 0,16-0,18 (0,17±0,01) g/hari. Produksi yang diperoleh adalah 7.862; 10.699; dan 12.163 kg/petak, masing-masing pada padat penebaran 750; 1.000 dan 1.250 ekor/m2. Nilai rasio konversi pakan 1,4; 1,36; 1,55 dan kebutuhan listrik 3,2; 2,5; 2,4 kw/kg udang serta kebutuhan air 2,24; 1,66; 1,60 m3/kg udang. Biaya produksi udang terendah adalah Rp. 30.526/ kg udang pada padat penebaran 1.000 ekor/m2 dengan laba operasional sebesar Rp. 630.687.094/th. Padat penebaran 1.000 ekor/m2 menghasilkan kinerja lebih baik sehingga disarankan menjadi acuan padat penebaran untuk budidaya udang vaname superintensif. Teknologi ini memiliki potensi dampak terhadap lingkungan perairan, sehingga perlu dilengkapi sarana Instalasi Pengolah Air Limbah (IPAL) untuk pengolah air buangan tambak.In order to increase of brackishwater pond’s productivity, an effort can be reached through high stocking density of shrimp accompanied by application of high quality inputs and supported by an appropriate technology. Three different stocking densities, were applied i.e., 750; 1,000; and 1,250 ind/m2. The shrimp were reared for 105 days in three ponds with sizing of 1,000 m2 each and the water depth of 1.8 m facilitated with aeration systems consisted of paddlewheels, root blower, submersible water pump, automatic feeder, central drainage, collector drainage and waste water treatment plan. The capacity aeration systems was 500 kg of shrimp biomass/HP. The results showed that all stocking densities produced the similar final body weight of shrimp which ranged between 15.48 to 16.30 (15.78±0.45) g/shrimp with daily growth rates were 0.16-0.18 (0.17±0.01) g/day. The total harvested shrimps from each stocking density were 7,862; 10,699, 12,163 kg/pond, respectively. The feed conversion ratio was 1.4, 1.36, and 1.55, whereas consumed electricities were 3.2, 2.5, and 2.4 kw/kg shrimp and water demands were 2.24, 1.66, and 1.60 m3/kg harvested shrimp. The lowest production cost was IDR 30,526/kg harvested shrimp which was spent for stocking density of 1,000 ind/m2, whereas the annual profit was IDR 630,687,094. The stocking density of 1,000 ind/m2 showed high performances, and then eventually is recommended for the L. vannamei super-intensive aquaculture. This technology is potential in affecting the adjacent environment, however the impacts might be minimized through the application of deploying wastewater treatment plan.


2020 ◽  
Vol 29 (2) ◽  
pp. 137-145
Author(s):  
Md Mizanur Rahman ◽  
Gouri Mondal ◽  
MD Marufujjaman Mithu ◽  
Md Golam Rabbane ◽  
Md Ghulam Mustafa

Experiment was conducted to evaluate the high density culture of Mystus cavasius and its effects on growth performances, survival rate and proximate composition of the fish in recirculating aquaculture system. Fishes were cultured at 571, 714, 857 and 1000 fries/m3 as the initial stocking density. No significant differences (p > 0.05) were found in specific growth rate, average daily gain, food conversation ratio, protein efficiency ratio, condition factor (k) and survival rate (%) of fish under the culture period of 120 days among the different stocking densities. The average survival rate was ≥ 99% among the treatments at the end of the culture period. The moisture contents were between 77.10 and 77.75%, ash content was 2.58 and 2.61%, crude protein was 15.86 and 16.07%, crude lipid was 5.45 and 5.68% with no significant differences (p > 0.05) among the treatments. There were no significant variations in DO, TAN, NO3-N, NO2-N and pH among the treatments during culture. This study showed that stocking density of at least 1000 fries/m3 was the best option as the total gain was highest compared to other lower densities under similar facilities. Dhaka Univ. J. Biol. Sci. 29(2): 137-145, 2020 (July)


Author(s):  
SN Mona ◽  
S Sultana ◽  
KK Ahmed ◽  
N Khan ◽  
KA Huq ◽  
...  

The research was carried out with small indigenous fish species (SIS) named mola (Amblypharyngadan mola) in monoculture system to intensify production in farmers’ homestead ponds with 50% household women involvement during June to December, 2016. Brood mola was stocked at the rate of 2, 4 and 6 m-2 with three replications in each treatment. Supplementary feed (27.1% protein) consisted of commonly available agricultural by-products was provided daily at the rate of 2% of the total biomass along with natural feed as mola is planktivorous species. Seven different water quality parameters were measured fortnightly and found within cultivable range. Gross production of mola were obtained 520.59±23.4, 599.06±258.87, 1063.94±42.87 kg ha-1 from T1, T2 and T3, respectively after 4 months’ culture period. The yield of T3 was significantly greater (P < 0.05) than T1 and T2. The highest gross production was obtained in T3 where stocking density was 6 brood mola m-2. Int. J. Agril. Res. Innov. Tech. 9(2): 23-29, December 2019


2009 ◽  
Vol 8 (2) ◽  
pp. 199 ◽  
Author(s):  
S. Sarah ◽  
. Widanarni ◽  
Agus Oman Sudrajat

<p>This experiment was conducted to study the stocking density effect on growth and survival rate of giant gouramy seed in the aquarium. Fish were stocked at the density of 2.5; 5.0; 7.5 and 10 individual/l with average initial weight and length were 0.013 g and 5.56 mm, respectively. Fish were fed with silkworm (<em>Tubifex</em> sp.) <em>ad libitum</em> in the morning and evening for 22 days of experiment. Aquarium was siphoned and water was changed everyday for maintaining good water quality. Result showed that survival rate remain high in all treatment (93.5 - 95.5%). Increasing stocking density from 2.5 to 10 individual/I did not affect survival rate. On the other hand growth rate and feed efficiency decreased, while fish yield increased. Stocking density of 2,5 individual/l gave  the highest daily growth rate, individual growth rate, growth in length and feed efficiency of 12.94%, 0.0081 g/day, 16.84 mm and 12.51%; respectively. The highest value for fish yield (0,0360 g/l/day) was obtained from stocking density of 10 individual/l</p> <p>Key words: stocking density, seed, survival rate, growth rate, giant gouramy <em>Osphrenemus goramy</em></p> <p> </p> <p>ABSTRAK</p> <p>Keterbatasan pengadaan benih ikan gurame (<em>Osphronemus gourame </em>Lac.) disebabkan oleh tidak seimbangnya jumlah benih yang tersedia dengan kebutuhan usaha pembesaran. Teknik pembenihan yang digunakan sampai saat ini relatif sederhana dengan cara tradisional. Pemeliharaan gurame secara terkontrol di akuarium dapat menjawab tantangan dalam teknologi pembenihan gurame sekaligus sebagai sarana pola budidaya secara bertahap yang sedang berkembang saat ini. Produksi yang tinggi akan dicapai dengan pemeliharaan pada kepadatan yang tinggi. Pada keadaan lingkungan yang baik dan pakan yang mencukupi, peningkatan kepadatan akan disertai dengan peningkatan hasil. Jumlah ikan yang ditebar pada penelitian ini disesuaikan berdasarkan perlakuan yaitu 2,5; 5,0; 7,5 dan 10 ekor/l. Pengamatan terhadap ikan dilakukan sampai hari ke-22. Hasil pengamatan menunjukkan bahwa peningkatan kepadatan 2,5 - 10 ekor/l pada pemeliharaan benih gurame di akuarium mempengaruhi pertumbuhan, hasil dan efisiensi pakan, namun tidak berpengaruh terhadap kelangsungan hidup. Dengan meningkatnya padat penebaran, laju pertumbuhan dan efisiensi pakan semakin menurun, sedangkan hasil (<em>yield</em>) semakin meningkat. Padat penebaran 2,5 ekor/l menghasilkan laju pertumbuhan harian, laju pertumbuhan individu, pertumbuhan panjang mutlak dan efisiensi pakan tertinggi, masing-masing mencapai 12,94 %, 0,0081 g/hari, 16,84 mm dan 12,51 %. Sedangkan nilai tertinggi untuk hasil (<em>yield</em>) sebesar 0,0360 g/l/hari diperoleh pada padat penebaran 10,0 ekor/l.</p> <p>Kata kunci : padat tebar, pembenihan, gurame dan <em>Osphronemus gouramy</em></p>


PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245224
Author(s):  
Caroline Robé ◽  
Katrin Daehre ◽  
Roswitha Merle ◽  
Anika Friese ◽  
Sebastian Guenther ◽  
...  

The colonization of broilers with extended-spectrum β-lactamase- (ESBL-) and plasmid-mediated AmpC β-lactamase- (pAmpC-) producing Enterobacteriaceae has been extensively studied. However, only limited data on intervention strategies to reduce the colonization throughout the fattening period are available. To investigate practically relevant management measures for their potential to reduce colonization, a recently published seeder-bird colonization model was used. Groups of 90 broilers (breed Ross 308) were housed in pens under conventional conditions (stocking of 39 kg/m2, no enrichment, water and feed ad libitum). Tested measures were investigated in separate trials and included (I) an increased amount of litter in the pen, (II) the reduction of stocking density to 25 kg/m2, and (III) the use of an alternative broiler breed (Rowan x Ranger). One-fifth of ESBL- and pAmpC- negative broilers (n = 18) per group were orally co-inoculated with two E. coli strains on the third day of the trial (seeder). One CTX-M-15-positive E. coli strain (ST410) and one CMY-2 and mcr-1-positive E. coli strain (ST10) were simultaneously administered in a dosage of 102 cfu. Colonization of all seeders and 28 non-inoculated broilers (sentinel) was assessed via cloacal swabs during the trials and a final necropsy at a target weight of two kilograms (= d 36 (control, I-II), d 47 (III)). None of the applied intervention measures reduced the colonization of the broilers with both the ESBL- and the pAmpC- producing E. coli strains. A strain-dependent reduction of colonization for the ESBL- producing E. coli strain of ST410 by 2 log units was apparent by the reduction of stocking density to 25 kg/m2. Consequently, the tested management measures had a negligible effect on the ESBL- and pAmpC- colonization of broilers. Therefore, intervention strategies should focus on the prevention of ESBL- and pAmpC- colonization, rather than an attempt to reduce an already existing colonization.


2021 ◽  
Author(s):  
Musa Sarıca ◽  
Koray Karakoç ◽  
Kadir Erensoy

Abstract This study aimed to determine the changes in the performance, welfare, and productivity level of broiler chickens reared at various group sizes and non-grouped (single flock) under intensive field conditions. Two treatments were tested as a concept in all trials. In the GF (grouped flock) house, the grouping was applied at 6000 (GF 1 or large GF), 4000 (GF 2 or medium GF), and 3000 (GF 3 or small GF) broilers in the first, second and third trials, respectively, without changing the stocking density. In the SF (single flock) house, classical intensive rearing was applied without grouping the whole 20000 broilers during each of 3 trials (SF 1, 2, and 3). The results showed that large and medium GF chickens had higher BW than SFs at slaughter age. In SF 2 chickens, only HB level was significantly higher in the second trial (1.39 vs. 1.17). In the third trial, the FPD, HB, and BB scores of SF 3 chickens were higher when compared to small GFs at 1.85 to 1.41, 1.48 to 1.22, and 2.27 to 1.89, respectively. Chickens reared in GFs had more BW, FI, and better FCR and EPEF values when compared to SF chickens. BW, FI, and EPEF were observed to be lower—while FCR was higher—when SF chickens were compared to GFs. Upon considering the EPEF value along with welfare parameters, it has been observed that rearing chickens in groups of 3000 may increase productivity by up to 54.8% according to intensive conditions.


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