scholarly journals EFFECTS OF PH SOAKING SOLUTIONS AND HYPOXIA/ANAEROBIC TREAMENT ON GABA ACCUMULATION IN GERMINATED MUNG BEAN (VIGNA RADIATA L.)

2017 ◽  
Vol 55 (2) ◽  
pp. 150 ◽  
Author(s):  
Trung Nhat Truong ◽  
Nguyen Tuong Danh ◽  
Dong Thi Anh Dao
Keyword(s):  
Anaerobic Condition ◽  
Vigna Radiata ◽  
Mung Bean ◽  
Ph Value ◽  
Aerobic Condition ◽  
Culture Conditions ◽  
Anaerobic Conditions ◽  
Gaba Production ◽  
Gaba Content ◽  
Gaba Accumulation

This study investigated the accumulation of γ-aminobutyric acid (GABA) in mung bean sprouts (Vigna Radiata L.) during germination under hypoxia/anaerobic conditions. Raw mung bean seeds were soaked in water at 35oC for 8 hours, dried and germinated under aerobic or hypoxia/anaerobic conditions at 35oC or 40oC for 0, 6, 12, 24, 36 and 48 hours.  Free amino acid and GABA contents were measured during the course of germination. Effect of soaking pH (5.3, 6.3 and 7.3) on GABA content of germinated mung beans under hypoxia/anaerobic condition was also investigated. The other objective of this study was to optimize the culture conditions of GABA production in germinated mung bean. A significant increase (p < 0.05) of GABA content was found in mung bean samples germinated under hypoxia/anaerobic condition. As this result, the combination of soaking pH 6.3 and 12 hour germination at 35oC was found to yield the highest GABA content (1519.07±19.58ppm).  This figure was 1.41 times as high as the content in aerobic condition and 25.32 times as high as the analysis of the raw mung beans. And experimental analysis showed that the optimal condition with aeration treatment for GABA accumulation in germinated mung bean was at a temperature of 36.6oC, time of 14.5 h and a pH value of 5.83; the highest GABA yield was 1638.67ppm, which was 27.77 times higher than raw seeds. In conclusion, germinated mung bean is a good source of GABA and hypoxia/anaerobic condition in combination with soaking pH, germination time and temperature can increase significantly GABA content in germinated mung beans.

Oxygen concentration affects frequency and range of transconjugants for the incompatibility (Inc) P-1 and P-7 plasmids pBP136 and pCAR1

2020 ◽  
Vol 85 (4) ◽  
pp. 1005-1015
Author(s):  
Kentaro Ochi ◽  
Maho Tokuda ◽  
Kosuke Yanagiya ◽  
Chiho Suzuki-Minakuchi ◽  
Hideaki Nojiri ◽  
...  
Keyword(s):  
Oxygen Concentration ◽  
Anaerobic Condition ◽  
Environmental Samples ◽  
Aerobic Condition ◽  
Recipient Strain ◽  
Donor Strain ◽  
Anaerobic Conditions ◽  
Filter Mating ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

ABSTRACT The frequency of transconjugants were compared for the incompatibility (Inc) P-1 and P-7 plasmids pBP136 and pCAR1 under aerobic and anaerobic conditions. Filter mating assays were performed with one donor strain and one recipient strain using different donors of Pseudomonas and recipient strains, including Pseudomonas, Pantoea, and Buttiauxella. Under anaerobic condition, frequencies of transconjugants for both plasmids were 101-103-fold lower than those under aerobic condition regardless of whether aerobically or anaerobically grown donors and recipients were used. To compare the transconjugant ranges under aerobic and anaerobic conditions, conjugation was performed between the donor of pBP136 and recipient bacteria extracted from environmental samples. Several transconjugants were uniquely obtained from each aerobic or anaerobic condition. Our findings indicate that a plasmid can differently spread among bacteria depending on the oxygen concentrations of the environment.

scholarly journals Incubation Studies on Exchangable Zn for Varying Levels of added Zn Under Aerobic and Anaerobic Conditions in Grey Terrace Soils, Non Calcarious Floodplain Soils and Calcarious Floodplain Soils

2013 ◽  
Vol 9 (1-2) ◽  
pp. 9-15 ◽  
Author(s):  
KH Talukder ◽  
IU Ahmed ◽  
MS Islam ◽  
MS Islam ◽  
M Asaduzzaman ◽  
...  
Keyword(s):  
Anaerobic Condition ◽  
Early Stage ◽  
Aerobic Condition ◽  
Anaerobic Conditions ◽  
Floodplain Soil ◽  
Zinc Fractions ◽  
Floodplain Soils ◽  
Soil Zn ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

Fractions studies were done to know how the zinc applied to different soils was distributed in to various fractions when the soils incubated under aerobic and anaerobic condition. The added zinc provided significant increase in exchangeable Zn both under aerobic and anaerobic conditions although anaerobic condition gave lower results than aerobic condition. The higher results were obtained at early stage of incubation and it gradually reduced as the incubation period proceeded to 90 days. These results showed all most similar trends for all the soils under study. In general, added zinc showed significantly higher results to the different fractions of soil Zn both under anaerobic and aerobic incubation with very few exceptions. The highest amount of added Zn (12 kg/ha) always produced greater results than the lower doses. Only exchangeable Zn was found higher in the 1st measurement at 15 DAI then gradually decreased but in other cases, gradual increase in zinc fractions was seen as the incubation study proceed to longer duration provided with very few exceptions. In many cases, the exchangeable-Zn found higher only at 15 DAI but sharply reduced at 30 DAI. In general, the Gray Terrace Soil produced the highest results followed by Non Calcareous Gray Floodplain and the lowest results were observed in Dark Grey Floodplain & Brown Floodplain Soil. DOI: http://dx.doi.org/10.3329/jsf.v9i1-2.14643 J. Sci. Foundation, 9(1&2): 9-15, June-December 2011

scholarly journals Study of boosting gamma-Aminobutyric acid (GABA) content in germinated mung bean under hypoxia-anaerobic condition and evaluating the loss of GABA after boiling

2016 ◽  
Vol 19 (4) ◽  
pp. 88-96
Author(s):  
Trung Nhat Truong ◽  
Dao Thi Anh Dong
Keyword(s):  
Moisture Content ◽  
Water Temperature ◽  
Anaerobic Condition ◽  
Mung Bean ◽  
Loss Rate ◽  
Raw Material ◽  
Aminobutyric Acid ◽  
Gamma Aminobutyric Acid ◽  
Optimal Conditions ◽  
Gaba Content

In this study, we investigated some factors effected on GABA stimulation under hypoxia-anaerobic condition and evaluated the loss rate of GABA amount after boiling and drying. These factors include pH soaking water, temperature and time germination. We also carried out the germinated optimization based on above factors. The results showed the optimal conditions included: temperature was 36.6oC, time was 14.5 hours and pH was 5.83. The highest GABA was 1638.67ppm and higher 27.55 times than raw material. Seeds (after germinating) were boiled at 80oC in 15 minutes and dried at 50oC in 3 hours. Finally, we ground it to recover the germinated mung bean powder which moisture content was 6.13% and GABA content was 273.76ppm - 4.6 times higher than the raw powder.

The Influences of Aerobic and Anaerobic Conditions on PHB and Glycerin Yields in the Process of Lignin Degradation by Pseudomonas stutzeri P156

2013 ◽  
Vol 634-638 ◽  
pp. 1170-1174 ◽  
Author(s):  
Zhi Wang ◽  
Wei Zi Qin ◽  
Shuai Shuai Bao ◽  
Xiong Chen ◽  
Feng Lian Zhang ◽  
...  
Keyword(s):  
Anaerobic Condition ◽  
Degradation Rate ◽  
Lignin Degradation ◽  
Lignin Content ◽  
Aerobic Condition ◽  
Pseudomonas Stutzeri ◽  
Anaerobic Conditions ◽  
The Cost ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

The effects of aerobic and anaerobic conditions on polyhydroxybutyrate (PHB) and glycerin yields during the course of lignin degradation were studied with Pseudomonas stutzeri P156. The lignin degradation rate was 22.5% and 15%, respectively, under aerobic and anaerobic condition. However, the gradation rate/biomass (YL/X) was nearly no differences that diversified from 0.9% to 3%. In addition, the maximal anaerobic yield of PHB and glycerin yield was 0.42 g/g and 56.3 ug/ml, which was 176.6% and 36.7% higher than that under aerobic condition, respectively. Interestingly to point out, anaerobic lignin degradation rate was decreased by 33.3% relative to the aerobic one, but lignin content did decreased by 15% at the cost of higher yields of PHB and glycerin, which suggested biosyntheses of PHB and glycerin could balance the redox potential under anaerobic condition, facilitate lignin degradation by Pseudomonas stutzeri P156, persistently, and meet the process demands of straw-based anaerobic methane fermentation.

Linking changes in chlorophyll a fluorescence with drought stress susceptibility in mung bean [ Vigna radiata (L.) Wilczek]

2021 ◽  
Author(s):  
Hussan Bano ◽  
Habib‐ur‐Rehman Athar ◽  
Zafar Ullah Zafar ◽  
Hazem M. Kalaji ◽  
Muhammad Ashraf
Keyword(s):  
Drought Stress ◽  
Chlorophyll A ◽  
Chlorophyll A Fluorescence ◽  
Vigna Radiata ◽  
Mung Bean ◽  
Stress Susceptibility

Nanoplastic ingestion induces behavioral disorders in terrestrial snails: trophic transfer effects via vascular plants

2020 ◽  
Vol 7 (3) ◽  
pp. 975-983 ◽  
Author(s):  
Yooeun Chae ◽  
Youn-Joo An
Keyword(s):  
Vigna Radiata ◽  
Mung Bean ◽  
Vascular Plants ◽  
Trophic Transfer ◽  
Achatina Fulica ◽  
Transfer Effects ◽  
Terrestrial Environment ◽  
Plastic Debris ◽  
Terrestrial Snails ◽  
African Giant Snail

This study investigated the transfer of plastic debris in a terrestrial environment from the soil to a plant (the mung bean, Vigna radiata), and then to a consumer (the African giant snail, Achatina fulica).

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