scholarly journals The Efficiency of Rectal Fluid Therapy in Moderately Dehydrated Horses

2018 ◽  
Vol 75 (1) ◽  
pp. 73
Author(s):  
Patricia AȘTELEAN ◽  
Eva DIUGAN ◽  
Cristian MOLNAR ◽  
Orsolya SÁRPATAKI ◽  
Răzvan CODEA ◽  
...  
Keyword(s):  
Fluid Therapy ◽  
Blood Count ◽  
Animal Species ◽  
Complete Blood Count ◽  
Rectal Administration ◽  
Response To Therapy ◽  
Blood Samples ◽  
Before And After ◽  
Life Threatening ◽  
Laboratory Investigations

Dehydration and hydro-electrolytic imbalances are relatively common and life threatening conditions in many animal species including horses. In the present study, we highlighted the efficacy of rectal rehydration in horses, nowadays less commonly used. The study was performed on eight non-pregnant mares (Romanian semigreus and Lipiţan breeds), from Beclean horse stud farm, affected by mild dehydration. The method consists in rectal administration in the form of a high enema of a balanced polyionic solution by using an esophageal tube; the solution contains water, sodium chloride and potassium chloride. Blood samples were collected on EDTA and procoagulant vacutainers, before and after fluid therapy. Laboratory investigations include complete blood count and electrolytes measurement, total protein, albumin, and creatinine. Expectedly, dehydrated animals shown borderline elevated hematocrit associated with mild relative hyperproteinemia and hypernatremia, and elevated uremia levels. In all cases, the parameters were restored in response to therapy, and no side effects were recorded.

scholarly journals Path of least resistance: how computerised provider order entry can lead to (and reduce) wasteful practices

2018 ◽  
Vol 7 (2) ◽  
pp. e000345 ◽  
Author(s):  
Joseph Choi ◽  
Cori Rebecca Atlin
Keyword(s):  
Blood Count ◽  
Complete Blood Count ◽  
Clinical Care ◽  
Work Flow ◽  
Quality Improvement Initiative ◽  
Order Entry ◽  
Clinical Value ◽  
Careful Planning ◽  
Order Sets ◽  
Before And After

BackgroundComputerised provider order entry (CPOE) is rapidly becoming the mainstay in clinical care and has the potential to improve provider efficiency and accuracy. However, this hinges on careful planning and implementation. Poorly planned CPOE order sets can lead to undetected errors and waste. In our emergency department (ED), lactate dehydrogenase (LDH) was bundled into various blood work panels but had little clinical value.ObjectivesThis quality improvement initiative aimed to reduce unnecessary LDH testing in the ED.MethodsA group of ED physicians reviewed CPOE blood work panels and uncoupled LDH in conditions where it was deemed not to provide any clinically useful information. We measured the daily number of LDH tests performed before and after its removal. We tracked the frequency of other serum tests as controls. We also analysed the number of add-on LDH (ie, to add LDH to samples already sent to the lab) as a balancing measure, since this can disrupt work flow and delay care.ResultsThrough this intervention, we reduced the number of LDH tests performed by 69%, from an average of 75.1 tests per day to 23.2 (P<0.0005). The baseline controls did not differ after the intervention (eg, a complete blood count was performed 197.7 and 196.1 times per day preintervention and postintervention, respectively (P=0.7663)). There was less than one add-on LDH per day on average.ConclusionsCPOE care templates can be powerful in shaping behaviours and reducing variability. However, close oversight of these panels is necessary to prevent errors and waste.

scholarly journals CORRELATION BETWEEN WDF, WNR, AND RET ABNORMAL SCATTERGRAM DETECTED BY SYSMEX XN-1000 AND PARASITEMIA OF MALARIA PATIENTS IN MERAUKE HOSPITAL

2019 ◽  
Vol 26 (1) ◽  
pp. 91
Author(s):  
Merylin Ranoko ◽  
Aryati Aryati ◽  
Arifoel Hajat
Keyword(s):  
General Hospital ◽  
Health Problem ◽  
Gold Standard ◽  
Blood Count ◽  
Complete Blood Count ◽  
Cross Sectional Study ◽  
Response To Therapy ◽  
Sectional Study ◽  
Cross Sectional ◽  
Degree Of Severity

Malaria remains a health problem in Indonesia. Microscopic examination with Giemsa staining is the gold standard for diagnosing malaria. The density of parasites correlates with the degree of severity and response to therapy of malaria. Malaria-causing plasmodium can be detected by Sysmex XN-1000 which is marked by abnormalities in the WDF, WNR and RET scattergram. This research aimed to determine the correlation of WDF, WNR and RET abnormal scattergram detected by Sysmex XN-1000 and the parasitemia index of malaria at the Merauke General Hospital. This was a cross-sectional study with observational approach conducted between November 2017 – February 2018 at the Merauke General Hospital. Positive malaria samples were stained with Giemsa, their parasitemia index was calculated, routine complete blood count using Sysmex XN-1000 was performed, and the scattergram abnormalities were then analyzed. There were 65 positive malaria samples as follows: P.falciparum (35%), P.vivax (60%), P.ovale (3.1%), and P.malariae (1.5%), but the species did not correlate with parasitemic index (p=0.691). Abnormalities of WDF and WNR scattergram were predominantly found than RET scattergram (80% vs. 27.7%). P.vivax predominantly caused abnormalities of the WDF and WNR scattergram in 36 of 39 samples (92.3%), whereas P.falciparum predominantly caused abnomalities of the RET scattergram in 14 of 23 samples (60.9%). There was 95% positivity of an abnormality in WDF/WNR/RET scattergram with a cut-off of > 5,0165.5/µL. There was correlation between WDF, WNR, RET scattergram detected by Sysmex XN-1000 and the parasitemia index.

scholarly journals Effect of Integrated Ayurveda and Yoga Therapy (IAYT) on remission management of non-Hodgkin lymphoma: A single case study

2020 ◽  
Vol 1 (1) ◽  
pp. 13
Author(s):  
Tripathi Satyam ◽  
Shenoy Kiran ◽  
Munamarty Bhavani
Keyword(s):  
Hodgkin Lymphoma ◽  
Perceived Stress ◽  
Blood Count ◽  
Complete Blood Count ◽  
Response To Therapy ◽  
Post Intervention ◽  
Current Case ◽  
Yoga Therapy ◽  
Non Hodgkin Lymphoma

Non-Hodgkin lymphomas are lymphoid malignant neoplasms and the prognosis depends on the staging and response to therapy. Stress is an independent factor which can affect the prognosis of the disease. The purpose of the current case study was to evaluate the impact of Yoga and Ayurveda as complementary therapy on the prognosis of the disease and the effect of the same on perceived stress level. A 36 year patient diagnosed with relapsed Non-Hodgkin Lymphoma (NHL) after receiving 3 rounds of chemotherapy and bone marrow transplant visited Union Yoga Ayurveda Clinic in Singapore for therapy. Perceived stress, complete blood count and hemoglobin were assessed pre and post intervention. A 6 month follow up was also done with similar evaluations. The patient was given holistic Yoga therapy and Ayurveda herbal treatment including patrapindasweda (herbal compress) and pichu(herbal soaked gauze pieces). The patient reported improvement in day to day energy levels and immunity with increase in WBC count. The perceived stress scores also showed distinct improvement post intervention. The current case study suggests that the integrative yoga and Ayurveda therapy was feasible and showed improvement in stress and complete blood count in patients with Non-Hodgkin lymphomas.

scholarly journals Impact of Fasting on Complete Blood Count Assayed in Capillary Blood Samples

2017 ◽  
Vol 48 (4) ◽  
pp. 357-361 ◽  
Author(s):  
Barbara K Kościelniak ◽  
Aleksandra Charchut ◽  
Magdalena Wójcik ◽  
Krystyna Sztefko ◽  
Przemysław J Tomasik
Keyword(s):  
Blood Count ◽  
Complete Blood Count ◽  
Capillary Blood ◽  
Blood Samples

Evaluation of the accuracy of complete blood count for insufficient blood samples

2016 ◽  
Vol 54 (11) ◽  
Author(s):  
Lin-Lin Pan ◽  
Chia-Hsin Shih ◽  
Chih-Yi Lee ◽  
Fang-Yi Chuang ◽  
Cheuk-Kwan Sun
Keyword(s):  
Blood Count ◽  
Complete Blood Count ◽  
Blood Samples

scholarly journals Effects of Theileria Orientalis Infection on Health Status and Productivity of Dairy Cows Reared inside Barns

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 650
Author(s):  
Yuki Fukushima ◽  
Tomoya Minamino ◽  
Yoko Mikurino ◽  
Kazuyuki Honkawa ◽  
Yoichiro Horii ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Blood Count ◽  
Complete Blood Count ◽  
Dairy Farm ◽  
The Other ◽  
Pcr Analysis ◽  
Dry Period ◽  
Theileria Orientalis ◽  
Blood Samples ◽  
Longitudinal Observational Study

The objective of the present study was to investigate the effects of Theileria orientalis on the severity of anemia, the prevalence of disease within 21 days after calving and productivity in cows raised inside barns. This longitudinal observational study, which was conducted on a commercial dairy farm in Japan, involved 627 Holstein cows subjected to PCR analysis for T. orientalis. In study 1, we collected blood samples from 156 sick cows within 21 days after calving, and we found the prevalence of T. orientalis infection to be 65.4%. In study 2, we randomly selected 471 cows during the dry period and collected blood samples to conduct PCR analysis for T. orientalis and determined the prevalence of T. orientalis infection to be 69.0%. Compared with the values for the T. orientalis-uninfected group, the T. orientalis-infected cows had significantly decreased hemoglobin concentrations and hematocrit, but there were no differences in the other complete blood count indexes between the two groups. In addition, there were no differences in productivity and the prevalence of major diseases between the T. orientalis-infected and uninfected cows. In summary, T. orientalis had few effects on anemia, productivity and the health of cows raised inside a barn.

scholarly journals Investigating the genetic architecture of disease resilience in pigs by genome-wide association studies of complete blood count traits collected from a natural disease challenge model

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Xuechun Bai ◽  
Tianfu Yang ◽  
Austin M. Putz ◽  
Zhiquan Wang ◽  
Changxi Li ◽  
...  
Keyword(s):  
Blood Cell ◽  
Genetic Architecture ◽  
Blood Count ◽  
Complete Blood Count ◽  
Association Studies ◽  
Genome Wide Association ◽  
Genome Wide Association Studies ◽  
Genome Wide ◽  
Before And After ◽  
Genomic Regions

Abstract Background Genetic improvement for disease resilience is anticipated to be a practical method to improve efficiency and profitability of the pig industry, as resilient pigs maintain a relatively undepressed level of performance in the face of infection. However, multiple biological functions are known to be involved in disease resilience and this complexity means that the genetic architecture of disease resilience remains largely unknown. Here, we conducted genome-wide association studies (GWAS) of 465,910 autosomal SNPs for complete blood count (CBC) traits that are important in an animal’s disease response. The aim was to identify the genetic control of disease resilience. Results Univariate and multivariate single-step GWAS were performed on 15 CBC traits measured from the blood samples of 2743 crossbred (Landrace × Yorkshire) barrows drawn at 2-weeks before, and at 2 and 6-weeks after exposure to a polymicrobial infectious challenge. Overall, at a genome-wise false discovery rate of 0.05, five genomic regions located on Sus scrofa chromosome (SSC) 2, SSC4, SSC9, SSC10, and SSC12, were significantly associated with white blood cell traits in response to the polymicrobial challenge, and nine genomic regions on multiple chromosomes (SSC1, SSC4, SSC5, SSC6, SSC8, SSC9, SSC11, SSC12, SSC17) were significantly associated with red blood cell and platelet traits collected before and after exposure to the challenge. By functional enrichment analyses using Ingenuity Pathway Analysis (IPA) and literature review of previous CBC studies, candidate genes located nearby significant single-nucleotide polymorphisms were found to be involved in immune response, hematopoiesis, red blood cell morphology, and platelet aggregation. Conclusions This study helps to improve our understanding of the genetic basis of CBC traits collected before and after exposure to a polymicrobial infectious challenge and provides a step forward to improve disease resilience.

scholarly journals Differential and diagnostic significance of monocytosis in treatment of moderate forms of COVID-19

2021 ◽  
Author(s):  
M. Shperling ◽  
E. Shperling ◽  
A. Kovalev ◽  
A. Vlasov ◽  
A. Polyakov ◽  
...  
Keyword(s):  
Antibiotic Therapy ◽  
Bacterial Infection ◽  
Blood Count ◽  
Complete Blood Count ◽  
Venous Blood ◽  
Clinical Signs ◽  
Response To Therapy ◽  
Control Group ◽  
Group 2 ◽  
Group 1

Treatment of COVID-19-associated pneumonia in the overwhelming majority of cases is accompanied by empirical prescription of antibiotic therapy. According to a number of studies carried out, the addition of a bacterial infection in this disease is noted less often than in other viral pneumonias, in particular, caused by the influenza virus. In addition, the occurrence of leukocytosis in response to therapy with glucocorticosteroids (GCS) is often perceived as an attachment of bacterial flora and is the reason for initiating antibiotic therapy. Therefore, an urgent task is the correct interpretation of leukocytosis in response to GCS therapy in COVID-19. The purpose of the work was to study the dynamics of changes in the number of leukocytes, neutrophils and monocytes of venous blood in patients with moderate COVID-19 with systemic use of GCS. Also we aimed to determine the differences in these indicators between the group of patients with indirect signs of bacterial infection and the group of patients receiving GCS. We analyzed the indicators of the complete blood count of 154 patients in the temporary infectious diseases hospital in the “PATRIOT” Park of the Moscow region with confirmed moderate form of COVID-19. The comparison group (1) consisted of 128 patients without clinical signs of bacterial infection and leukocytosis on admission, who were prescribed GCS therapy. The control group (2) consisted of 26 people who, upon admission, showed signs of a bacterial infection - a cough with purulent sputum in combination with neutrophilic leukocytosis. The dynamics of cells in venous blood was assessed in patients of group (1) before the start, 3 and 6 days after the start of GCS therapy. We also compared the number of leukocytes, neutrophils and monocytes between patients with developed leukocytosis from group (1) in response to GCS therapy and group (2). As a result of the study, an increase in the number of leukocytes, neutrophils and monocytes was revealed according to the data of the complete blood count test in patients of the group (1) on days 3 and 6 of GCS therapy. All patients with developed leukocytosis (103 people) had no clinical signs of bacterial infection. In patients with developed leukocytosis from group (1), an increase in the number of monocytes was revealed (0.90 (0.84; 1.02) on day 3 of GCS and 0.94 (0.87; 1.26) on day 6 of GCS) compared with group (2) (0.61 (0.50; 0.71)), p <0.001. The number of leukocytes and neutrophils did not differ between the groups. The appearance of monocytosis when taking GCS may be due to the presence of macrophage activation syndrome in the pathogenesis of COVID-19 and, therefore, increased activation of monocytes. The use of GCS in this case leads to inhibition of the migration of monocytes to the inflammation area and to the stimulation of the production of their anti-inflammatory pool (M2 cells) by the bone marrow. This fact causes an increase in the number of monocytes in the peripheral blood. Monocytosis in response to GCS therapy can be a differential diagnostic criterion between glucocorticoid-induced leukocytosis and the addition of a bacterial infection. This may be one of the factors influencing the decision to prescribe antibiotic therapy, and may also be a criterion for the effectiveness of GCS immunosuppressive therapy in COVID-19, which requires further study.

scholarly journals Investigating the genetic architecture of disease resilience in pigs by genome-wide association studies of complete blood count traits collected from a natural disease challenge model

2020 ◽  
Author(s):  
Xuechun Bai ◽  
Tianfu Yang ◽  
Austin Putz ◽  
Zhiquan Wang ◽  
Changxi Li ◽  
...  
Keyword(s):  
Blood Cell ◽  
Genetic Architecture ◽  
Blood Count ◽  
Complete Blood Count ◽  
Association Studies ◽  
Genome Wide Association ◽  
Genome Wide Association Studies ◽  
Genome Wide ◽  
Before And After ◽  
Genomic Regions

Abstract BackgroundGenetic improvement for disease resilience is anticipated to be a practical method to improve efficiency and profitability of the pig industry, as resilient pigs maintain a relatively undepressed level of performance in the face of infection. However, multiple biological functions are known to be involved in disease resilience and this complexity means that the genetic architecture of disease resilience remains largely unknown. Here, we conducted genome-wide association studies (GWAS) of 465,910 autosomal SNPs for complete blood count (CBC) traits that are important in an animal’s disease response. The aim was to identify the genetic control of disease resilience.ResultsUnivariate and multivariate single-step GWAS were performed on fifteen CBC traits measured from the blood samples of 2743 crossbred (Landrace × Yorkshire) barrows drawn at 2-weeks before, and at 2 and 6-weeks after exposure to a polymicrobial infectious challenge. Overall, at a genome-wise false discovery rate of 0.05, five genomic regions located on Sus scrofa chromosome (SSC) 2, SSC4, SSC9, SSC10, and SSC12, were significantly associated with white blood cell traits in response to the polymicrobial challenge, and nine genomic regions on multiple chromosomes (SSC1, SSC4, SSC5, SSC6, SSC8, SSC9, SSC11, SSC12, SSC17) were significantly associated with red blood cell and platelet traits collected before and after exposure to the challenge. By functional enrichment analyses using Ingenuity Pathway Analysis (IPA) and literature review of previous CBC studies, candidate genes located nearby significant single-nucleotide polymorphisms were found to be involved in immune response, hematopoiesis, red blood cell morphology, and platelet aggregation.ConclusionsThis study helps to improve our understanding of the genetic basis of CBC traits collected before and after exposure to a polymicrobial infectious challenge and provides a step forward to improve disease resilience.

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