scholarly journals Morphological and Molecular Characterization and Relationships of Turkish Local Eggplant Heirlooms

2015 ◽  
Vol 43 (1) ◽  
pp. 100-107 ◽  
Author(s):  
Hatice Filiz BOYACI ◽  
Volkan TOPCU ◽  
Akin TEPE ◽  
Isilay Karasahin YILDIRIM ◽  
Mehmet OTEN ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Similarity ◽  
Rapd Markers ◽  
Molecular Data ◽  
Morphological Data ◽  
Good Source ◽  
High Genetic Diversity ◽  
Local Genotypes ◽  
Morphological And Molecular Data ◽  
Morphological And Molecular Characterization

A total of 38 eggplant genotypes, of which 32 were heirloom accessions collected from different regions of Burdur province five were different local genotypes from other provinces, and one was a cultivar, were used as reference in this study. The phylogenetic relationships among these heirlooms were evaluated using 40 morphologic descriptors and five randomly amplified polymorphic RAPD markers. The horizontal dendrograms were created by using UPGMA with both morphologic and molecular data. Burdur heirloom accessions showed high genetic diversity based on morphological and molecular data. The genetic similarity rates ranged from 0.29 to 0.91 according to the morphological data, and ranged from 0.84 to 0.98 according to the molecular data. Molecular data generated by RAPD method, compared to morphological data, were insufficient to reveal genetic diversity. Therefore, in order to confirm genetic variations, studies based on other molecular methods are necessary. The regional genetic populations include a wide eggplant genetic diversity which can be good source for the breeding studies performed in the future.

scholarly journals Genetic Similarity of Commerson’s Anchovy across Segara Anakan Cilacap Assessed Using Randomly Amplified Polymorphic DNA (RAPD) Markers

Jurnal Biota ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 42-50
Author(s):  
Muhammad Khoerol Anam ◽  
Adi Amurwanto ◽  
Kusbiyanto Kusbiyanto ◽  
Hendro Pramono ◽  
M Husein Sastranegara ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Similarity ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Differences ◽  
Population Genetic Study ◽  
Randomly Amplified Polymorphic Dna ◽  
High Genetic Diversity ◽  
Impressive Result

Segara Anakan areas can be divided into three different regions according to their salinity. Salinity differences suggested that Commerson’s anchovy population in that area can be divided into three subpopulations due to genetic differences. Genetic differences among subpopulation can be assessed through a population genetic study using random amplified polymorphic DNA. This study aims to evaluate the genetic variation and differences of Commerson's anchovy (Stolephorus commersonnii) collected at three different water salinities in Segara Anakan estuary Cilacap Indonesia. Total genomic DNA was isolated using the Chelex method. Genetic diversity and differences were assessed using RAPD markers and were analyzed statistically using an analysis of molecular variance, as implemented in Arlequin software.  The results showed that high genetic diversity was observed within the subpopulations. However, no significant genetic differences were observed among subpopulations which indicate genetic similarity. A high number of offspring are likely to cause high genetic variation within subpopulations.  Adult and larvae migration is the cause of genetics similarity across Segara Anakan. Another impressive result is that water salinity did not affect the genetic characteristic of Commerson,s anchovy. Genetic similarity of Commerson’s anchovy indicates that Segara Anakan forms a single genetic conservation unit.

scholarly journals Genetic diversity in Sesamum indicum L.

2019 ◽  
Vol 48 (3) ◽  
pp. 497-506
Author(s):  
Maini Bhattacharjee ◽  
Adil Iqbal ◽  
Sanjana Singha ◽  
Disharee Nath ◽  
SH Prakash ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Geographical Origin ◽  
Genotypic Diversity ◽  
Molecular Data ◽  
Sesamum Indicum ◽  
Morphological Data ◽  
Oilseed Crop ◽  
Morphological And Molecular Data ◽  
Genetic Diversity Study ◽  
Diversity Study

Sesame (Sesamum indicum L.), is the most ancient and important oilseed crop for its good quality edible oil. Much variability among genotypes exists in sesame which helps the breeder to choose good parents in hybridization. In the present study, 30 genotypes were studied for 12 morphological traits. Correlation analysis exhibited that seed yield per plant was positive and significantly correlated with capsules per plant and 1000-seed weight. Apart from morphological data, initial genotyping was conducted for 32 microsatellites but finally only polymorphic primers were selected for genetic diversity study. Estimated polymorphism information content (PIC) value ranged from 0.07 to 0.87 and SSR 24 showed to be highest at 0.87. The dendrogram was constructed using the software NTSYS Pc Ver. 2.20 based on morphological and molecular data. Geographical diversity did not corroborate with genotypic diversity as the cluster composition revealed that genotypes belonging to different geographical origin grouped in the same cluster.

scholarly journals Genetic Diversity of Castor Bean (Ricinus communis L.) Revealed by ISSR and RAPD Markers

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 457
Author(s):  
HyokChol Kim ◽  
Pei Lei ◽  
Aizhi Wang ◽  
Shuo Liu ◽  
Yong Zhao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Castor Bean ◽  
Genetic Similarity ◽  
Rapd Markers ◽  
Ricinus Communis ◽  
Similarity Coefficient ◽  
Morphological Data ◽  
Oilseed Crop ◽  
Ricinus Communis L ◽  
Polymorphic Bands

Castor (Ricinus communis L.), known as castor oil plant or castor bean, is a non-edible oilseed crop. In the present study, the genetic diversity among 54 samples (3 wild and 51 cultivated) collected worldwide was evaluated using inter-simple sequence repeats (ISSRs) and random amplified polymorphic DNA (RAPD) markers. A total of 9 ISSR primers produced 83 high-resolution bands with 61 (74.53%) as polymorphic. The percentage of polymorphic bands per primer and the genetic similarity coefficient ranged from 54.55% (UBC-836) to 100% (UBC-808) and from 0.74 to 0.96, respectively. A total of 11 out of 20 RAPD primers amplified unique polymorphic products with an average percentage of polymorphic bands of 60.98% (56 polymorphic bands out of a total of 90 bands obtained). The percentage of polymorphic bands per primer ranged from 25% (OPA-02 and B7) to 90.91% (B21) with the genetic similarity coefficient ranging from 0.73 to 0.98. The unweighted pair group method with arithmetic averages (UPGMA) dendrogram using two molecular markers divided 54 castor genotypes into three groups. Furthermore, based on morphological data, all 54 castor varieties were grouped into three main clusters. The genetic diversity analysis based on two molecular makers showed that most varieties from China were closely related to each other with three varieties (GUANGDONGwild, ZHEJIANGWild, and HANNANWild) belonging to a wild group separated from most of the cultivated castor samples from China, India, France, and Jordan. These results suggested that the cultivated castor contains a narrow genetic base. Accordingly, we recommend that wild castor genetic resources be introduced for breeding novel castor varieties. Furthermore, the Vietnam, Malaysia, Indonesia, and Nigeria accessions were clustered into the same group. The results of principal coordinate analysis (PCoA) and UPGMA cluster analysis were consistent with each other. The findings of this study are important for future breeding studies of castor.

scholarly journals Genetic polymorphism in brazilian microcystis spp. (Cyanobacteria) toxic and non-toxic through RFLP-PCR of the cpcBA-IGS

2009 ◽  
Vol 52 (4) ◽  
pp. 901-909 ◽  
Author(s):  
Maria do Carmo Bittencourt-Oliveira ◽  
Maristela Casé Costa Cunha ◽  
Ariadne do Nascimento Moura
Keyword(s):  
Genetic Diversity ◽  
Genetic Polymorphism ◽  
Genetic Variability ◽  
Intergenic Spacer ◽  
Single Species ◽  
Molecular Data ◽  
High Genetic Diversity ◽  
Toxic Blooms ◽  
Morphological And Molecular Data ◽  
Brazilian Populations

The escalating occurrence of cyanobacterial toxic blooms demands a better understanding of genetic variability as an auxiliary expedient in species identification, collaborating with the monitoring of water destined to public supply. This study aimed at the unraveling of genetic polymorphism in the toxic and nontoxic strains of Microcystis (Cyanobacteria) species, isolated from diverse Brazilian localities through the RFLP-PCR technique applied to the c-phycocyanin encoding operon and its intergenic spacer (cpcBA-IGS). Eighteen strains belonging to M. aeruginosa, M. panniformis, M. protocystis and M. wesenbergii, plus two other unidentified strains, were analyzed by means of the morphological and molecular data. The molecular data constituted three groups with low similarity values unrelated to the geographical origin, toxicity or morphospecies. A high genetic variability among the studied populations was unveiled by the results. Brazilian populations of Microcystis spp. displayed high genetic diversity when compared to those from Australia, Japan, United States and Europe. This ample genetic diversity could be observed through the diverse eletrophoretic profiles obtained among the strains from a single species. The presence of toxic and non-toxic strains was observed in the same species, as M. aeruginosa.

scholarly journals Comparative study of genetic diversity using RAPD markers in Salvia (Lamiaceae)

Caryologia ◽  
2021 ◽  
Author(s):  
Majid KHAYYATNEZHAD ◽  
Sayed Afzal Shah2
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Genetic Difference ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Data ◽  
Shannon Index ◽  
Neighbor Joining ◽  
The World ◽  
Morphological And Molecular Data ◽  
High Degree

Salvia has a high degree of environmental compatibility and is widespread around the world, especially in tropical and temperate regions. It is represented by 61 species in Iran including 19 endemic species. Salvia species are mostly shrubs or subshrubs, occasionally herbs, typically perennial, sometimes biennial or annual, and often aromatic. The genus has high medicinal, commercial and horticultural value. It is the largest and one of the taxonomically complicated genus of Lamiaceae. To determine the genetic diversity and understand the species’ limits within the Iranian Salvia, we produced both morphological and molecular data using 145 randomly collected plants representing 30 species from 18 provinces of Iran. A total of 107 reproducible bands were generated by 10 of 25 random amplified polymorphic DNA (RAPD) primers, with an average of 10.7 bands/primer and 44% polymorphism. Largest number of effective alleles (Ne), genetic diversity (H), and Shannon Index (I) were shown by S. reuterana. Our data depicted highest similarity between S. suffruticosa and S. hydrangea and lowest between S. aristata and S. oligphylla. Salvia limbata showed relatively low level of genetic variation. Finally, the Neighbor Joining (NJ) trees based on RAPD markers data divided the populations into two different clusters, indicating their genetic difference which is discussed in details.

scholarly journals Morphological and molecular characterization of three new Parastenocarididae (Copepoda: Harpacticoida) from caves in Southern Italy

2020 ◽  
Author(s):  
Maria Cristina Bruno ◽  
Vezio Cottarelli ◽  
Federico Marrone ◽  
Rosario Grasso ◽  
Erika Stefani ◽  
...  
Keyword(s):  
Southern Italy ◽  
Molecular Study ◽  
Molecular Data ◽  
Morphological Data ◽  
Ecological Knowledge ◽  
Mitochondrial Coi ◽  
Copepodid Stage ◽  
Morphological And Molecular Data ◽  
Morphological And Molecular Characterization

We describe three new parastenocaridid: Cottarellicaris sanctiangeli Bruno & Cottarelli sp. nov., Stammericaris vincentimariae Bruno & Cottarelli sp. nov. and Proserpinicars specincola Bruno & Cottarelli sp. nov., collected in the pools of five different caves located in Calabria (Southern Italy). We conducted a phylogenetic analysis based on the mitochondrial COI and ribosomal 18S sequences of C. sanctiangeli sp. nov. and S. vincentimariae sp. nov., and of four more species of Stammericaris and one Proserpinicaris available from literature. Based on the molecular study, the specimens of C. sanctiangeli sp. nov. are clearly separated from the species belonging to the closely-related genus Stammericaris. The morphological and molecular data indicate that the genera belonging to the two subfamilies Parastenocaridinae and Fontinalicaridinae form two monophyletic and distinct clades, thus supporting their status. We also provide insights on the genus Proserpinicaris based on morphological data only; in particular, the most important synapomorphic character of the genus, i.e., the taxonomic value of the hyaline structure inserted on the anterior surface of the male leg 4 basis, is discussed based on the development of the P4 endopod, as observed in the last copepodid stage of some species of this genus. Finally, we widen the biogeographic and ecological knowledge of the three genera.

scholarly journals Genetic diversity assessment in Indian cultivated pea (Pisum sativum L.) varieties using RAPD markers

2015 ◽  
Vol 7 (1) ◽  
pp. 316-323 ◽  
Author(s):  
Pravas Ranjan Kole ◽  
Manoj Kumar Sharma ◽  
Sanjay Kumar ◽  
Rakesh Kumar
Keyword(s):  
Genetic Diversity ◽  
Genetic Similarity ◽  
Rapd Markers ◽  
Germplasm Management ◽  
High Genetic Diversity ◽  
Pisum Sativum L ◽  
Diversity Assessment ◽  
High Level ◽  
Maximum Similarity ◽  
Clustering Pattern

In the present study, the genetic diversity and inter-relationships among 12 varieties of pea were analyzed by using RAPD markers. A total of 118 bands were generated with 20 RAPD primers, of which 107 bands were polymorphic (90.81%). The PIC value ranged from 0.602-0.863 with an average of 0.754. High level of polymorphism and low genetic similarity within pea varieties suggested that they have a high level of genetic diversity. Unique RAPD fragments (700bp-1500bp) were also observed in five varieties i.e., AP-01, Aparna, Uttra, Rachna and Rachna-1D. In the dendrogram, 12 varieties were broadly grouped into 2 main clusters consisting of 5 (Cluster-I) and 4 (Cluster-II) varieties, respectively, while other three varieties i.e., KPMR-522, Aparna and AP-03 were out of group. In cluster-I, Ambika and AP-01 and Rachna and Vikas showed 62% and 58% similarities whereas, HUDP-15 grouped with 56% similarities with rest two varieties. In cluster II, Uttara and Prakash showed maximum similarity (65%) whereas, Rachna1-D showed (58%) similarities with them. KPMR-400 showed 52% similarity in this group. KPMR-400, Aparna and AP-03 were more diverse in comparison to others. A two-dimensional plot generated from principal co-ordinate analysis of RAPD data also supported the clustering pattern of dendrogram. This study indicated the presence of high genetic diversity among pea varieties, which could be used for developing core collections of pea germplasm for breeding and germplasm management purposes.

Analysis of the differences between Syntormon pallipes and S. pseudospicatus (Diptera: Dolichopodidae): morphological and molecular data

2019 ◽  
Vol 28 (2) ◽  
pp. 305-316
Author(s):  
M.A. Chursina ◽  
I.Ya. Grichanov
Keyword(s):  
Dna Analysis ◽  
Distinct Species ◽  
Molecular Data ◽  
Wing Shape ◽  
Morphological Data ◽  
12S Rrna ◽  
Separate Species ◽  
Geometric Morphometric ◽  
Geometric Morphometric Analysis ◽  
Morphological And Molecular Data

The recent catalogues of the family Dolichopodidae considered Syntormon pallipes (Fabricius, 1794) and S. pseudospicatus Strobl, 1899 as separate species. In this study, we used three approaches to estimate the significance of differences between the two species: molecular analysis (COI and 12S rRNA sequences), analysis of leg colour characters and geometric morphometric analysis of wing shape. The morphological data confirmed the absence of significant differences between S. pallipes and S. pseudospicatus found in the DNA analysis. Significant differences in the wing shape of two species have not been revealed. Hence, according to our data, there is no reason to consider S. pseudospicatus as a distinct species.

Genetic Diversity among Calamus brandisii Becc. (Arecaceae) Populations in Kerala Part of Western Ghats

2021 ◽  
Vol 43 (1) ◽  
pp. 38-42
Author(s):  
Kavungal Priya ◽  
◽  
Indira . ◽  
Vadakkethil Balakrishnan Sreekumar ◽  
Renuka . ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Western Ghats ◽  
Rapd Markers ◽  
Gene Diversity ◽  
Issr Markers ◽  
High Genetic Diversity ◽  
Western Ghats Of India ◽  
The Mean ◽  
Two Parameters ◽  
Two Populations

Calamus brandisii Becc. is one of the endemic slender rattans found in the Western Ghats of India. The genetic diversity of two main populations available in Kerala was investigated using 20 RAPD and 9 ISSR markers. Two parameters viz., gene diversity and genetic diversity within and among populations were analyzed. ISSR analysis showed quite high genetic diversity in Pandimotta compared to Bonacaud population whereas in RAPD markers both these populations were moderately diverse. The percentage of total genetic differentiation (Gst) among two populations is relatively higher than the mean Gst value indicating high genetic diversity within the populations. The genetic distance between these two populations was 0.1739 with ISSR markers and 0.1971 with RAPD markers. Because of its high genetic diversity, Pandimotta population can be treated as an important population of gene diversity with potentially useful genes. This may be included in the high priority reservoir for genetic conservation also.

Revision of Autolytinae (Syllidae: Polychaeta)

Zootaxa ◽  
2004 ◽  
Vol 680 (1) ◽  
pp. 1 ◽  
Author(s):  
ARNE NYGREN
Keyword(s):  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Data Sets ◽  
Nominal Species ◽  
Strict Consensus Tree ◽  
Incertae Sedis ◽  
Nomina Dubia ◽  
Morphological And Molecular Data ◽  
Combined Data

Autolytinae is revised based on available types, and newly collected specimens. Out of 170 nominal species, 18 are considered as incertae sedis, 43 are regarded as junior synonyms, and 25 are referred to as nomina dubia. The relationships of Autolytinae is assessed from 51 morphological characters and 211 states for 76 ingroup-taxa, and 460 molecular characters from mitochondrial 16S rDNA and nuclear 18S rDNA for 31 ingroup-taxa; outgroups include 12 non-autolytine syllid polychaetes. Two analyses are provided, one including morphological data only, and one with combined morphological and molecular data sets. The resulting strict consensus tree from the combined data is chosen for a reclassification. Three main clades are identified: Procerini trib. n., Autolytini Grube, 1850, and Epigamia gen. n. Proceraea Ehlers, 1864 and Myrianida Milne Edwards, 1845 are referred to as nomen protectum, while Scolopendra Slabber, 1781, Podonereis Blainville, 1818, Amytis Savigny, 1822, Polynice Savigny, 1822, and Nereisyllis Blainville, 1828 are considered

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