scholarly journals AM Fungi Influences the Photosynthetic Activity, Growth and Antioxidant Enzymes in Allium sativum L. under Salinity Condition

2010 ◽  
Vol 2 (4) ◽  
pp. 64-71 ◽  
Author(s):  
Mahesh BORDE ◽  
Mayura DUDHANE ◽  
Paramjit Kaur JITE
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Enzymes ◽  
Allium Sativum ◽  
Growth Parameters ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Dry Weight ◽  
Salinity Level ◽  
Antioxidant Enzyme Activities ◽  
Moderate Salinity

Potential of Arbuscular mycorrhizal (AM) fungi in alleviating adverse salt effects on growth was tested in garlic (Allium sativum L.). Towards this objective we analyzed the AM root colonization and the activities of various antioxidant enzymes like peroxidase, catalase, and superoxide dismutase at 0, 100, 200 and 300 mM salinity levels. The activities of all the antioxidant enzymes studied were found to be increased in AM garlic plants. Antioxidant activity was maximum in 100 and 200 mM NaCl (sodium chloride) in AM and non-AM plants. Proline accumulation was induced by salt levels and it was more in leaves as well as roots of AM plants as compared to non-AM plants, this indicating that mycorrhiza reduced salt injury. Growth parameters of garlic plants like leaf area, plant fresh and dry weight and antioxidant enzyme activities were higher at moderate salinity level. This work suggests that the mycorrhiza helps garlic plants to perform better under moderate salinity level by enhancing the antioxidant activity and proline content as compared to non-AM plants.

scholarly journals Diversity of endomycorrhizal fungi and their synergistic effect on the growth of Acacia catechu Willd.

2009 ◽  
Vol 55 (No. 10) ◽  
pp. 461-468 ◽  
Author(s):  
V. Parkash ◽  
A. Aggarwal
Keyword(s):  
Growth Parameters ◽  
Trichoderma Viride ◽  
Phosphorus Uptake ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Dry Weight ◽  
Rhizosphere Microflora ◽  
Beneficial Effects ◽  
Acacia Catechu ◽  
Slow Growing

The diversity of arbuscular mycorrhizal (AM) fungi of <I>Acacia catechu</I> Willd. was studied. Dominant AM spores, the bacterium <I>Rhizobium</I> sp. along with the fungus <I>Trichoderma viride</I> were isolated from the rhizosphere of <I>A. catechu</I> and mass-produced in laboratory. The co-inoculation effect of <I>Glomus mosseae, Glomus fasciculatum</I>, mixed AM (<I>Glomus</I> spp. [except <I>G. mosseae, G. fasciculatum</I>] with <I>Acaulospora</I> spp., <I>Sclerocystis</I> spp. and <I>Gigaspora</I> spp.), <I>Rhizobium</I> sp. and <I>Trichoderma viride</I> was studied as exerted on the growth of <I>A. catechu</I> seedlings. All inoculated seedlings showed improved seedling growth compared to the control. Inoculated seedlings had a pronounced effect on all growth parameters such as height, fresh and dry weight of roots and shoots, AM spore count, per cent mycorrhizal colonization in roots and root nodule number in comparison with uninoculated seedlings. Phosphorus uptake was also higher in inoculated seedlings than in the control. This study provides a good scope for commercially utilizing the efficient strains of AM fungi for beneficial effects with other beneficial rhizosphere microflora in the primary establishment of slow growing seedlings ensuring better survival and improved growth.

Arbuscular mycorrhizal fungi improve the growth and nodulation of the annual legume messina (Melilotus siculus) under saline and non-saline conditions

2012 ◽  
Vol 63 (2) ◽  
pp. 164 ◽  
Author(s):  
B. A. L. Wilson ◽  
G. J. Ash ◽  
J. D. I. Harper
Keyword(s):  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Dry Weight ◽  
Salinity Level ◽  
P Nutrition ◽  
Uninoculated Control ◽  
Uptake Of Nutrients ◽  
Saline Land

Messina [Melilotus siculus (Turra) Vitman ex. B. D Jacks] is a salt- and waterlogging-tolerant annual legume that could be highly productive on saline land. Arbuscular mycorrhizal (AM) fungi form a symbiotic relationship with the majority of terrestrial plant species, and improved productivity of plants inoculated with AM fungi under saline conditions has been attributed to the increased uptake of nutrients such as phosphorus (P). However, the mycorrhizal status of M. siculus under saline or non-saline conditions is unknown, as is the role of AM in improved nutrition and nodulation. In this study, the role of AM fungi in growth improvement and nodulation of M. siculus was examined in saline and non-saline soil. The M. siculus plants were inoculated with either a single AM species or mixed AM species, or remained uninoculated, and were grown at three levels of sodium chloride (NaCl) (0, 80, and 250 mm NaCl). AM-inoculated plants had significantly greater nodulation than plants that did not receive AM inoculum, regardless of salinity level. Plants inoculated with mixed AM species at 250 mm NaCl showed improved survival (90%) compared with the plants inoculated with single AM species or uninoculated control plants (30%). Within each salinity level, plants inoculated with mixed AM species had significantly greater dry weight than all other treatments. In addition, plants inoculated with mixed AM species had increased total uptake of P. It is likely that the increased growth observed in AM-inoculated M. siculus plants is due to improved P nutrition, showing the potential of AM fungi to enhance the growth of M. siculus on saline land.

scholarly journals Growth and Antioxidant Responses in Iron-Biofortified Lentil under Cadmium Stress

Toxics ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 182
Author(s):  
Ruchi Bansal ◽  
Swati Priya ◽  
Harsh Kumar Dikshit ◽  
Sherry Rachel Jacob ◽  
Mahesh Rao ◽  
...  
Keyword(s):  
Growth Parameters ◽  
Dry Weight ◽  
Cadmium Stress ◽  
Antioxidant Enzyme Activities ◽  
Limited Information ◽  
Cd Stress ◽  
Fresh Weight ◽  
Cd Uptake ◽  
Cd Tolerance ◽  
Controlled Conditions

Cadmium (Cd) is a hazardous heavy metal, toxic to our ecosystem even at low concentrations. Cd stress negatively affects plant growth and development by triggering oxidative stress. Limited information is available on the role of iron (Fe) in ameliorating Cd stress tolerance in legumes. This study assessed the effect of Cd stress in two lentil (Lens culinaris Medik.) varieties differing in seed Fe concentration (L4717 (Fe-biofortified) and JL3) under controlled conditions. Six biochemical traits, five growth parameters, and Cd uptake were recorded at the seedling stage (21 days after sowing) in the studied genotypes grown under controlled conditions at two levels (100 μM and 200 μM) of cadmium chloride (CdCl2). The studied traits revealed significant genotype, treatment, and genotype × treatment interactions. Cd-induced oxidative damage led to the accumulation of hydrogen peroxide (H2O2) and malondialdehyde in both genotypes. JL3 accumulated 77.1% more H2O2 and 75% more lipid peroxidation products than L4717 at the high Cd level. Antioxidant enzyme activities increased in response to Cd stress, with significant genotype, treatment, and genotype × treatment interactions (p < 0.01). L4717 had remarkably higher catalase (40.5%), peroxidase (43.9%), superoxide dismutase (31.7%), and glutathione reductase (47.3%) activities than JL3 under high Cd conditions. In addition, L4717 sustained better growth in terms of fresh weight and dry weight than JL3 under stress. JL3 exhibited high Cd uptake (14.87 mg g−1 fresh weight) compared to L4717 (7.32 mg g−1 fresh weight). The study concluded that the Fe-biofortified lentil genotype L4717 exhibited Cd tolerance by inciting an efficient antioxidative response to Cd toxicity. Further studies are required to elucidate the possibility of seed Fe content as a surrogacy trait for Cd tolerance.

scholarly journals Arbuscular Mycorrhizal Fungi (AMF) as Buffer for Heavy Metals Phytoextraction by Cucurbita maxima Duch. Grown on Crude Oil Contaminated Soil

2018 ◽  
Vol 3 ◽  
pp. 1-12
Author(s):  
Okon G. Okon ◽  
J.E. Okon ◽  
G.D.O. Eneh
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Crude Oil ◽  
Mycorrhizal Fungi ◽  
Growth Parameters ◽  
Chemical Properties ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Heavy Metal Accumulation ◽  
Cucurbita Maxima

This study evaluated the influence of Arbuscular Mycorrhizal (Rhizophagus irregularis) fungi inoculation (M) on the growth ofCucurbita maximaand as a buffer against phytoextraction of selected heavy metals (HM) (Zn, Cu, Cr, Cd and Pb) from a soil contaminated with crude oil (C). The experiment was set up using four soil treatments, each with three replicates C+ M-, C+ M+, C-M+ and C-M- (control without oil and inoculum). The shoot length, petiole length, number of nodes, leaf area and percentage germination ofC. maximawere significantly (p=0.05) reduced in uninoculated crude oil treatment (C+ M-), unpolluted mycorrhizal inoculated treatments (C-M+) showed remarkable response in growth parameters above the control (C-M-), while the polluted and inoculated treatment (C+ M+) showed significant (p=0.05) increase in growth parameters when compared to the polluted uninoculated treatment (C+ M-). Heavy metals analysis revealed a significant (p=0.05) difference in the heavy metal accumulation ofC. maxima. The heavy metals analyzed in this study are present thus inC. maxima; Zn>Cu>Cr>Pb>Cd. Crude oil polluted uninoculated treatment (C+ M-) recorded the highest concentrations of heavy metals than crude oil polluted inoculated (R. irregularis) treatment (C+ M+). Mycorrhizal inoculated unpolluted treatment (C-M+) and unpolluted uninoculated treatment (C-M-) indicated the lowest heavy metal concentrations. Inoculation withR. irregularissignificantly (p=0.05) reduced heavy metals uptake byC. maximaas observed in this study. Also, the negative effect of crude oil on AMF root colonization ofC. maximabyR. irregulariswas observed in polluted and inoculated treatment. HM often accumulate in the top layer of soil, therefore, are available for uptake by plants via roots, which is a major entry point of HM that ultimately affects different physiological processes. AM fungi can impinge on the chemical properties of heavy metals in the soil, their absorption by the host plant, and their allocation to different plant parts, affecting plant growth and the bioremediation process, thus making the AM fungi a suitable buffer for mitigating heavy metal stress onC. maxima.

Concentrations of K, Ca and Mg in maize colonized by arbuscular mycorrhizal fungi under field conditions

2002 ◽  
Vol 82 (3) ◽  
pp. 272-278 ◽  
Author(s):  
A. Liu ◽  
C. Hamel ◽  
A. Elmi ◽  
C. Costa ◽  
B. Ma ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Sandy Loam ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Dry Weight ◽  
Soil Fumigation ◽  
Soil P ◽  
Shoot Dry Weight ◽  
Loamy Sand Soil

Little attention has been paid to the effect of arbuscular mycorrhizal (AM) fungi on the uptake of nutrients that move mainly by mass flow. The objective of this study was to assess the possible contribution of indigenous AM fungi to the K, Ca and Mg nutrition of maize (Zea mays L.) as influenced by soil P levels and its impact on plant dry mass. The field experiment had a split plot design with four replicates. Treatments included soil fumigation status (fumigation and non-fumigation) and three levels of P fertilization (0, 60 and 120 kg P2O5 ha-1) in a loamy sand soil in 1997 and a fine sandy loam soil in 1998. Soil fumigati on with Basamid® was used to suppress indigenous AM fungi. Plants were sampled at four different growth stages (6-leaf stage, 10-leaf stage, tasseling and silking). Soil fumigation decreased shoot dry weight, but P fertilization increased shoot dry weight at most sampling times. When no P fertilizer was added, fumigation in the loamy sand soil reduced shoot K and Ca concentrations while, in contrast, in the fine sandy loam soil only Mg concentration was reduced by soil fumigation. The concentration of K in maize shoots was positively correlated (P < 0.05) with extraradicular hyphal length in both soils. The correlation between the abundance of extraradicular hyphae and the concentrations of Ca and Mg in maize shoots was significant only for soils where available Ca or Mg was relatively low. Arbuscular mycorrhizal fungi could increase corn biomass production and K, Ca and Mg uptake in soil low in these elements and low in P. These results indicate that the contribution of mycorrhizae to maize K, Ca and Mg nutrition can be significant in a field situation and that the extent of this contribution depends on the availability of these nutrients and of P in soils. Key words: Arbuscular mycorrhizal fungi, soil fumigation, extraradicular hyphae, uptake of K, Ca, and Mg, soil P levels, maize

scholarly journals Response of Arbuscular Mycorrhizal Fungi to Hydrologic Gradients in the Rhizosphere ofPhragmites australis(Cav.) Trin ex. Steudel Growing in the Sun Island Wetland

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Li Wang ◽  
Jieting Wu ◽  
Fang Ma ◽  
Jixian Yang ◽  
Shiyang Li ◽  
...  
Keyword(s):  
Host Plant ◽  
Mycorrhizal Fungi ◽  
Plant Biomass ◽  
Growth Parameters ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
The Sun ◽  
Vegetative Period ◽  
Hydrologic Gradients ◽  
Mycorrhizal Association

Within the rhizosphere, AM fungi are a sensitive variable to changes of botanic and environmental conditions, and they may interact with the biomass of plant and other microbes. During the vegetative period of thePhragmites australisgrowing in the Sun Island Wetland (SIW), the variations of AM fungi colonization were studied. Root samples of three hydrologic gradients generally showed AM fungi colonization, suggesting that AM fungi have the ability for adaptation to flooded habitats. There were direct and indirect hydrological related effects with respect to AM fungi biomass, which interacted simultaneously in the rhizosphere. Though water content in soil and reed growth parameters were both positively associated with AM fungi colonization, only the positive correlations between reed biomass parameters and the colonization could be expected, or both the host plant biomass and the AM fungi could be beneficial. The variations in response of host plant to the edaphic and hydrologic conditions may influence the effectiveness of the plant-mycorrhizal association. This study included a hydrologic component to better assess the role and distribution of AM fungi in wetland ecosystems. And because of that, the range of AM fungi was extended, since they actually showed a notable adaptability to hydrologic gradients.

Overexpression of MdIAA24 improves apple drought resistance by positively regulating strigolactone biosynthesis and mycorrhization

2020 ◽  
Author(s):  
Dong Huang ◽  
Qian Wang ◽  
Guangquan Jing ◽  
Mengnan Ma ◽  
Chao Li ◽  
...  
Keyword(s):  
Drought Stress ◽  
Positive Impact ◽  
Growth Parameters ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Land Plant ◽  
Exchange Capacity ◽  
Cortical Cells ◽  
Relative Water ◽  
Am Symbiosis

Abstract Most land plant species have the ability to establish a symbiosis with arbuscular mycorrhizal (AM) fungi. These fungi penetrate into root cortical cells and form branched structures (known as arbuscules) for nutrient exchange. We cloned the MdIAA24 from apple (Malus domestica) following its up-regulation during AM symbiosis. Results demonstrate the positive impact of the overexpression (OE) of MdIAA24 in apple on AM colonization. We observed the strigolactone (SL) synthesis genes, including MdD27, MdCCD7, MdCCD8a, MdCCD8b and MdMAXa, to be up-regulated in the OE lines. Thus, the OE lines exhibited both a higher SL content and colonization rate. Furthermore, we observed that the OE lines were able to maintain better growth parameters under AM inoculation conditions. Under drought stress with the AM inoculation, the OE lines were less damaged, which was demonstrated by a higher relative water content, a lower relative electrolytic leakage, a greater osmotic adjustment, a higher reactive oxygen species scavenging ability, an improved gas exchange capacity and an increased chlorophyll fluorescence performance. Our findings demonstrate that the OE of MdIAA24 in apple positively regulates the synthesis of SL and the formation of arbuscules as a drought stress coping mechanism.

Growth, anatomy and enzyme activity changes in maize roots induced by treatment of seeds with low-temperature plasma

Biologia ◽  
2012 ◽  
Vol 67 (3) ◽  
Author(s):  
Mária Henselová ◽  
Ľudmila Slováková ◽  
Michal Martinka ◽  
Anna Zahoranová
Keyword(s):  
Antioxidant Enzymes ◽  
Low Temperature ◽  
Growth Parameters ◽  
Dry Weight ◽  
Surface Barrier ◽  
Low Temperature Plasma ◽  
Sod Activity ◽  
Temperature Plasma ◽  
Maize Seeds ◽  
Maize Roots

AbstractThe seeds of Zea mays L. cv. KWS were exposed to low-temperature plasma (LTP) by using Diffuse Coplanar Surface Barrier Discharge (DCSBD) for 60 and 120 seconds respectively. Growth parameters, anatomy of roots and activity of some enzymes (CAT, G-POX, SOD and DHO) isolated from roots grown from the seeds treated by LTP were evaluated. Our results indicate that LTP treatment of maize seeds affects post-germination growth of seedlings and this effect depends on the duration of LTP treatment. LTP treatment in duration of 60 seconds significantly increased the length, fresh and dry weight of the roots. However, the increase in time of LTP treatment to 120 seconds had inhibitive effect on the studied growth parameters. The activities of all studied antioxidant enzymes significantly increased with the age of maize seedlings in control conditions. On the other hand the application of LTP resulted in small, mostly non significant changes in the activity of antioxidant enzymes. Significant decrease in CAT activity was observed both in 3 and 6-day old maize roots and G-POX activity in 3-day old maize roots grown from seeds exposed to LTP for 60 seconds. A small, significant increase was detected only in SOD activity in 3-day old maize roots grown from seeds treated with LTP for 120 seconds and in 6-day old maize roots treated with LTP for 60 seconds. Significantly higher DHO activity was determined in embryos isolated from seeds treated with LTP for 60 seconds. On the contrary, in roots the DHO activity decreased with the time of LTP treatment. LTP treatment of seeds did not affect the anatomy of maize roots and caused only minor changes in the isoenzyme composition of G-POX and SOD.

Contribution of arbuscular mycorrhizal (AM) fungi and hedgerow trees to the yield and nutrient uptake of cassava in an alley-cropping system

1998 ◽  
Vol 131 (1) ◽  
pp. 79-85 ◽  
Author(s):  
O. FAGBOLA ◽  
O. OSONUBI ◽  
K. MULONGOY
Keyword(s):  
Nutrient Uptake ◽  
Alley Cropping ◽  
Cropping System ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Dry Weight ◽  
P Uptake ◽  
Root Competition ◽  
Woody Legumes ◽  
Woody Legume

A field trial on alley-cropping was conducted at the University of Ibadan research farm in the 1990/91 cropping season to assess the contributions of arbuscular mycorrhizal (AM) fungi and hedgerow woody legumes to the yield and nutrient uptake of cassava (Manihot esculenta Crantz) as an intercrop in an infertile soil. The trial also investigated the influence of AM fungi on the interplanting of a non-nodulating woody legume Senna siamea (syn. Cassia siamea) with a nodulating woody legume (Leucaena leucocephala).AM contributions to cassava were greater than the hedgerow contributions, which demonstrated that AM associations are an essential component in the nutrition of cassava. In contrast to cassava, AM inoculation only influenced the leaf dry weight and uptake of nutrients of non-interplanted woody legumes but not the above-ground biomass and P uptake of interplanted woody legumes. However, non-inoculated interplanted Leucaena benefited more from indigenous AM fungi than the competing Senna. The negative contributions to the nutrient uptake (K, Ca and Mg) of cassava by hedgerows and the lack of response to AM inoculation in interplanted hedgerow woody legumes could be attributed to root competition among the different plant species growing in close proximity to each other. The present results show that cassava benefits more from AM association than Leucaena which in turn benefits more than Senna in an alley-cropping system.

scholarly journals Impact of Vermicompost and Chemical Fertilizer on Yield, Growth and Essential oil of Garlic (Allium sativum L.)

2015 ◽  
Vol 9 (4) ◽  
pp. 44-48
Author(s):  
Sajedeh Golmohammadzadeh ◽  
Sobhanallah Ghanbari ◽  
Seyede Roghaye Hosseini Valiki ◽  
Hasan Hasannia
Keyword(s):  
Essential Oil ◽  
Allium Sativum ◽  
Agronomic Traits ◽  
Growth Parameters ◽  
Dry Weight ◽  
Chemical Fertilizer ◽  
Chemical Fertilizers ◽  
Npk Fertilizers ◽  
Shoot Dry Weight ◽  
The Cost

In order to investigate the influence of vermicompost and chemical fertilizers on growth parameters and essential oil of garlic (Allium sativum), an experiment based on randomized complete blocks design with 7 treatments, 4 replications and 21 plots was carried out in Qaemshahr, Iran 2012.Treatments were included witness (control), 5, 10, 15, 20and25t/ha vermicompost and NPK fertilizers. Results showed that all agronomic traits were significantly affected by vermicompost and chemical fertilizers compared to the control. The maximum shoot wet weight and shoot dry weight recorded in chemical and vermicompost treatments, respectively.The maximum number ofbulbletsper plantand bulb dry weigh observed vermicompost treatment (20 t/ha). Among all treatment, vermicompost had highest effect on essential oil compared to chemical fertilizer. In general, 15 t/ha of vermicompost treatment compared to other treatments was appropriate for yield andproduction essential oil and reducing the cost of crop production.DOI: http://dx.doi.org/10.3126/ijls.v9i4.12675

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