The Enhancement of Soybean Growth and Yield in a Field Trial through Introduction of Mixtures of Bradyrhizobium japonicum, Bacillus sp. and Pseudomonas chlororaphis

The effects of plant growth promoting (PGP) bacteria on soybean growth and yield were tested in field conditions using four treatments: (A) Bradyrhizobium japonicum strain 526, combined with cell-free supernatants (CFS) of Bacillus sp. strain Q10 and Pseudomonas chlororaphis strain Q16; (B) B. japonicum 526 + Bacillus sp. Q10; (C) B. japonicum 526 + P. chlororaphis Q16 and (D) commercial fertilizer containing B. japonicum, which served as a control. The average values of dry weight per nodule and shoot dry weight had the maximum values in the B treatment. In dry shoots collected at the flowering stage, nitrogen and carbon content was similar across all treatments, while that of sulphur decreased in treatment A. Relative to the control (D), all treatments showed positive effects on pods number and grain mass per plant, with the best results yielded by treatment A. Nitrogen and sulphur content in grain were significantly higher in treatment C, whereas maximum carbon content was measured in treatment B. In the control, it was obtained the yield of 4,000 kgha-1, which was in accordance with data reported by the seed producer for the same growing conditions (the maximum value). The yields of 4,229, as well as 4,286 and 4,400 kg ha-1 were measured for variants C, B and A, respectively, which were statistically significant higher (5.73 to 10%) than the commercial fertilizer (D). The improvement in soybean growth and yield in the field trial achieved by addition of PGP strains Bacillus sp. Q10 and P. chlororaphis Q16 to B. japonicum 526 can result in more productive agricultural practices.

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Pectin-Rich Amendment Enhances Soybean Growth Promotion and Nodulation Mediated by Bacillus Velezensis Strains

Plants ◽

10.3390/plants8050120 ◽

2019 ◽

Vol 8 (5) ◽

pp. 120 ◽

Cited By ~ 7

Author(s):

Mohammad K. Hassan ◽

John A. McInroy ◽

Jarrod Jones ◽

Deepak Shantharaj ◽

Mark R. Liles ◽

...

Keyword(s):

Plant Growth ◽

Bradyrhizobium Japonicum ◽

Field Trial ◽

Growth Promotion ◽

Orange Peel ◽

Dry Weight ◽

Shoot Length ◽

Soybean Seeds ◽

Bacillus Velezensis ◽

Greenhouse Tests

Plant growth-promoting rhizobacteria (PGPR) are increasingly used in crops worldwide. While selected PGPR strains can reproducibly promote plant growth under controlled greenhouse conditions, their efficacy in the field is often more variable. Our overall aim was to determine if pectin or orange peel (OP) amendments to Bacillus velezensis (Bv) PGPR strains could increase soybean growth and nodulation by Bradyrhizobium japonicum in greenhouse and field experiments to reduce variability. The treatments included untreated soybean seeds planted in field soil that contained Bv PGPR strains and non-inoculated controls with and without 0.1% (w/v) pectin or (1 or 10 mg/200 μL) orange peel (OP) amendment. In greenhouse and field tests, 35 and 55 days after planting (DAP), the plants were removed from pots, washed, and analyzed for treatment effects. In greenhouse trials, the rhizobial inoculant was not added with Bv strains and pectin or OP amendment, but in the field trial, a commercial B. japonicum inoculant was used with Bv strains and pectin amendment. In the greenhouse tests, soybean seeds inoculated with Bv AP193 and pectin had significantly increased soybean shoot length, dry weight, and nodulation by indigenous Bradyrhizobium compared to AP193 without pectin. In the field trial, pectin with Bv AP193 significantly increased the shoot length, dry weight, and nodulation of a commercial Bradyrhizobium japonicum compared to Bv AP193 without pectin. In greenhouse tests, OP amendment with AP193 at 10 mg significantly increased the dry weight of shoots and roots compared to AP193 without OP amendment. The results demonstrate that pectin-rich amendments can enhance Bv-mediated soybean growth promotion and nodulation by indigenous and inoculated B. japonicum.

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Effect of Compost Application on the Growth of Acacia nilotica

Cercetari Agronomice in Moldova ◽

10.2478/cerce-2019-0007 ◽

2019 ◽

Vol 52 (1) ◽

pp. 66-73

Author(s):

H.M.U. Saqib ◽

I. Ahmad ◽

M.H.U. Rashid ◽

T.H. Farooq ◽

M. Asif ◽

...

Keyword(s):

Plant Height ◽

Biomass Production ◽

Field Trial ◽

Organic Fertilizer ◽

Acacia Nilotica ◽

Growth And Yield ◽

Positive Effects ◽

Forestry Practices ◽

Compost Application ◽

Pot Trial

Abstract Acacia nilotica is an important agroforestry specie, which is used in both compact and linear forms. The objective of the current study was to evaluate the effect of compost on the growth performance and biomass production of A. nilotica. Completely randomized design (CRD) was used to analyze the variations among several growth morphological traits. Two parallel trials, pot trial (seedlings), field trial (saplings) were conducted simultaneously. Compost and litter mixture were applied in mentioned trials. Following treatments were used: T0 – control; T1 - 25% of compost and 75% of nursery soil; T2 - mixture of 50% nursery soil and 50% compost; T3 - mixture of 75% compost and 25% of nursery soil; T4 - where 100% compost was applied. Increase in plant growth was observed with the increases in the amount of compost mixture. In field trial maximum plant height, shoot length, root length, rootshoot ratio and biomass production was observed when 100% compost level was applied, while minimum was observed without any compost appli-cation. In pot trials, the maximum plant height, rootshoot ratio and biomass production was recorded when 75% compost level was applied. Overall, Acacia performed better with 100% of compost application in field trail and 75% of compost application in pot trial. The results of this study demonstrated the positive effects of compost on the growth of Acacia. The seedling development was improved considerably with different levels having greater percentage of organic fertilizer and it was concluded that compost improves soil fertility and it should be used as organic fertilizer in farming and forestry practices for improving crop growth and yield.

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Influence of Seed Inoculation with Commercial Bacterial Inoculants (Bradyrhizobium Japonicum) on Growth and Yield of Soybean

Legume Research - An International Journal ◽

10.18805/lr-485 ◽

2019 ◽

Cited By ~ 1

Author(s):

W Jarecki ◽

D Bobrecka Jamro

Keyword(s):

Field Experiment ◽

Seed Yield ◽

Bradyrhizobium Japonicum ◽

Seed Weight ◽

Dry Weight ◽

Growth And Yield ◽

Plant Analysis ◽

Area Index ◽

Bacterial Inoculants ◽

Seed Inoculation

A field experiment was carried out with three bacterial inoculants: HiStick® Soy, Nitragina, Nitrazon and the control. Number and dry weight of nodules were significantly higher with inoculation of bacterial inoculants compared to the control. In 2017, the most pods per plant were obtained after the use of Nitrazon or HiStick® Soy, while in 2018, after the use of HiStick® Soy or Nitragina. In 2018, the highest 1000 seed weight was obtained after the use of Nitragina. In 2017, the seed yield was the most favourably affected by the inoculants HiStick®Soy (3.31 t ha-1) and Nitrazon (3.37 t ha-1) and in 2018, by HiStick® Soy (3.92 t ha-1) and Nitragina (3.87 t ha-1). High Soil Plant Analysis Development (SPAD) and Leaf Area Index (LAI) readings, were observed with HiStick® Soy.

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Bradyrhizobium japonicum mutants allowing improved nodulation and nitrogen fixation of field-grown soybean in a short season area

The Journal of Agricultural Science ◽

10.1017/s0021859602001995 ◽

2002 ◽

Vol 138 (3) ◽

pp. 293-300 ◽

Cited By ~ 12

Author(s):

H. ZHANG ◽

F. DAOUST ◽

T. C. CHARLES ◽

B. T. DRISCOLL ◽

B. PRITHIVIRAJ ◽

...

Keyword(s):

Nitrogen Fixation ◽

Total Nitrogen ◽

Bradyrhizobium Japonicum ◽

Dry Weight ◽

Growth And Yield ◽

Research Centre ◽

Soybean Production ◽

Nodule Number ◽

Nitrogen Yield ◽

Short Season

In short-season soybean production areas, low soil temperature is potentially a major factor limiting plant growth and yield. Although Bradyrhizobium japonicum strain 532 C is widely used in inoculants in Canadian soybean production, and USDA 110 is widely used in American soybean production, they are both inhibited by low temperatures. Genistein is an important plant-to-bacteria signal compound secreted by soybean roots. The addition of genistein has proven to be an effective means of generating increases in nitrogen fixation and yield but genistein is expensive. We used UV mutagenesis to make 10 mutants from USDA 110 that express nod genes without the addition of plant-to-bacteria signal compounds such as genistein. A field experiment was conducted at the Lods Agronomy Research Centre in southwestern Quebec in 1998 and 1999. The treatments consisted of factorial combinations of inoculant type (no inoculant (control) and inoculants containing the mutants Bj 30050, Bj 30051, Bj 30052, Bj 30053, Bj 30054, Bj 30055, Bj 30056, Bj 30057, Bj 30058, Bj 30059 and the wild type USDA110 or 532 C) and soybean cultivar (Bayfield and Maple Glen). The experiment was structured following a randomized complete block design with four blocks. Data were collected on nodule number, nodule dry weight, shoot nitrogen yield and total nitrogen fixation at five development stages. Averaged over the 2 years of the study, when pods were 2 cm long at one of the four uppermost nodes on the main stem (R4), inoculation with Bj 30055 and Bj 30058 resulted in greater nodule number (23 and 14%, respectively), nodule dry weight (16 and 13%, respectively), shoot nitrogen yield (19 and 21%, respectively) and total nitrogen fixation (10·9 and 12·7%, respectively) than 532 C, which is currently used in Canadian inoculants. The cultivar Bayfield produced more nodules, and higher nodule weight than Maple Glen, but there were no differences between the cultivars for shoot nitrogen yield and total nitrogen fixation.

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Aplikasi Bacillus sp. untuk mengendalikan penyakit layu fusarium pada tanaman tomat

Jurnal Agro ◽

10.15575/5397 ◽

2019 ◽

Vol 6 (2) ◽

pp. 144-152

Author(s):

Endang Mugiastuti ◽

Abdul Manan ◽

Ruth Feti Rahayuniati ◽

Loekas Soesanto

Keyword(s):

Fusarium Wilt ◽

Block Design ◽

Dry Weight ◽

Wilt Disease ◽

Growth And Yield ◽

Bacillus Sp ◽

Phenol Compounds ◽

Shoot Dry Weight ◽

Randomized Block Design ◽

Potential Alternative

Fusarium oxysporum merupakan penyebab penyakit layu fusarium yang dapat menurunkan produksi tomat. Pengendalian hayati dengan menggunakan bakteri antagonis seperti Bacillus sp. merupakan alternatif pengendalian yang potensial dan ramah lingkungan. Penelitian bertujuan untuk mengetahui kemampuan Bacillus sp. dalam mengendalikan penyakit layu fusarium pada tanaman tomat di lapangan. Percobaan menggunakan rancangan acak kelompok dengan 5 perlakuan dan 5 ulangan, meliputi: kontrol, Bacillus sp. B42, Bacillus sp. B64, gabungan Bacillus sp. B42 dan B64, serta fungisida. Hasil pengujian menunjukkan bahwa Bacillus sp. B.64 merupakan bakteri antagonis terbaik dalam menekan intensitas penyakit layu fusarium, karena dapat menunda masa inkubasi 15,76%, menekan intensitas penyakit 38,77%, meningkatkan kandungan fenol tanaman (tanin, saponin dan glikosida), serta meningkatkan pertumbuhan dan hasil tanaman dengan meningkatkan bobot kering tajuk 32,05%, bobot kering akar 15,23%, dan bobot buah per tanaman 46,48%.ABSTRACTFusarium oxysporum is the causal agent of fusarium wilt disease which decreases the production of tomatoes. Biological control managenement using bacterial antagonists is a potential alternative to prevent the infection of the disease. The aim of this research was to determine the ability of Bacillus sp. to control tomato fusarium wilt in the field. Randomized block design (RBD) experiment was used consisting of 5 treatments and 5 replications i.e. control, Bacillus sp. B42, Bacillus sp. B64, combination of Bacillus sp B42 + B64, and fungicide. The results showed that Bacillus sp. B64 was the best bacterial antagonist agent to control tomato wilt disease by delaying incubation period (15.76%), decreasing disease intensity (38.77%), increasing phenol compounds (tannin, saponin, glycosides) and improving plant growth and yield. Furthermore, the results showed the increasement of shoot dry weight to 32.05%, root dry weight to 15.23%, and yield to 46.48% as well.

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Endofit Bakteri Uygulamalarının Farklı Kuraklık Stresi Koşulları Altındaki Biber Fide Gelişimine Etkileri

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v9i7.1277-1282.4186 ◽

2021 ◽

Vol 9 (7) ◽

pp. 1277-1282

Author(s):

Aynur Sadak ◽

Abdulrahman Smail İbrahim ◽

Suat Şensoy

Keyword(s):

Drought Stress ◽

Plant Growth ◽

Root Length ◽

Growth Parameters ◽

Dry Weight ◽

Shoot Length ◽

Stem Diameter ◽

Bacillus Sp ◽

Bacterial Isolates ◽

Positive Effects

The study was carried out as a pot experiment in controlled conditions in order to reveal the effect of endophyte bacteria (EB) applications on the development of pepper seedlings grown under different drought stress. Mostar F1 was used as pepper variety. Applied endophyte bacterial isolates were Ochrobactrum sp. (CB36/1) and Bacillus sp. (CA41/1). The EB application was applied twice (first application 10 ml- second application 15 ml) at a density of 109 cfu/ml. All pots were irrigated regularly for 52 days after seed sowing. While irrigation was continued at 2-day intervals in control (B0) applications, four and eight days drougth stresses were applied in the second (B1) and last (B2) application. As plant growth parameters, stem diameter, shoot length and root length, shoot fresh and dry weights, leaf number, root fresh and root dry weight were examined. It has been determined that the effects of B1 and B2 stress applications on plant growth were generally negative. It has been determined that endophyte bacteria applications have relatively positive effects against the negativity caused by different drought stresses.

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Effect of different compost concentrations on the growth yield of Bombax Ceiba (Simal)

10.53365/nrfhh/144578 ◽

2022 ◽

Author(s):

Irfan Ahmad ◽

Muhammad Haroon U Rashid ◽

Shahid Nawaz ◽

Muhammad Asif ◽

Taimoor Hassan Farooq ◽

...

Keyword(s):

Plant Growth ◽

Field Experiment ◽

Organic Fertilizer ◽

Growth Yield ◽

Dry Weight ◽

Pot Experiment ◽

Growth And Yield ◽

Fresh Weight ◽

Positive Effects ◽

Compost Application

Bombax ceiba is an important agroforestry tree species widely distributed throughout the world. It has been extensively grown and planted for eras in hot and dry regions and high humidity zones of southern Asia. The main objective of this research was to evaluate the growth response of B. ceiba in response to different compost treatments. Different morphological traits (plant height, stem height, root length) and biomass (shoot fresh weight, shoot dry weight, root fresh weight, root dry weight and root/shoot ratio) were measured. Two experiments (pot experiment = seedlings) and (field experiment = saplings) were conducted simultaneously. Different compost treatments: (T0) = (Compost 0% + Soil 0%), (T1) = (Compost 25% + Soil 75%), (T2) = (Compost 50% + Soil 50%), (T3) = (Compost 75% + Soil 25%), (T4) = (Compost 100% + Soil 0%) were applied in the growing media. Results demonstrated that plant growth increased with the increment in compost application. In the pot experiment, B. ceiba exhibited its better growth under 75% of compost application, whereas in the field experiment, 100% compost was helpful for the best production of B. ceiba. Overall, positive effects of compost were observed for the growth of B. ceiba. The plant growth was increased greatly in response to the better content of organic fertilizer, and it was determined that compost enhances soil fertility. It should be implemented as organic fertilizer in agroforestry operations for optimizing plant growth and yield.

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Biomatriconditioning Benih dengan Rizobakteri untuk Meningkatkan Pertumbuhan dan Hasil Tanaman Sorgum (Sorghum bicolor L.)

Agrotekma Jurnal Agroteknologi dan Ilmu Pertanian ◽

10.31289/agr.v4i1.2949 ◽

2019 ◽

Vol 4 (1) ◽

pp. 52

Author(s):

Fitrianti Handayani ◽

Gusti Ayu Kade Sutariati ◽

Abdul Madiki

Keyword(s):

Sorghum Bicolor ◽

Pseudomonas Fluorescens ◽

Animal Husbandry ◽

Dry Weight ◽

Growth And Yield ◽

Bacillus Sp ◽

Rice Hulls ◽

Brick Powder ◽

Sorghum Bicolor L ◽

Range Test

<h1>The experiment was aimed to know the effect of seed biomatriconditioning with rhizobacteria to improve growth and yield of sorghum (Sorghum bicolor L.). The experiment was conducted in the Field of Animal Husbandry Agriculture Faculty, Haluoleo University from August up to November 2010. The experiment was arranged based on randomized completely design (CRD) which consisted of 5 seed biomatriconditioning with rhizobacteria treatments, namely: without seed biomatriconditioning (M0), seed biomatriconditioning with Bacillus sp. CKD061 + rice hulls charcoal (M1), seed biomatriconditioning with Bacillus sp. CKD061 + red brick powder (M2), seed biomatriconditioning with Pseudomonas fluorescens PG01 + rice hulls charcoal (M3), and seed biomatriconditioning with Pseudomonas fluorescens PG01 + red brick powder (M4). Every treatment was replicated 3 times, therefore, overall there were 15 experimental units. Data obtained were analized using analysis of variance and followed with Duncan’s Multiple Range Test. The result showed that seed treatment with biomatriconditioning using rhizobacteria gave significant effect in improving growth and yield of sorghum. Seed biomatriconditioning with sp. Bacillus CKD061 + red brick powder was the most effective treatment in increasing plant height, leaf area, dry weight of plant bomass, grain weight, and 1000 seed weight of sorghum.</h1>

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Impact of post-infiltration soil aeration at different growth stages of sub-surface trickle-irrigated tomato plants

International Agrophysics ◽

10.1515/intag-2015-0096 ◽

2016 ◽

Vol 30 (3) ◽

pp. 331-337 ◽

Cited By ~ 6

Author(s):

Yuan Li ◽

Zong-xia Jia ◽

Wen-Quan Niu ◽

Jing-wei Wang

Keyword(s):

Growth Period ◽

Dry Weight ◽

Growth And Yield ◽

Growth Stages ◽

Area Index ◽

Tomato Plants ◽

Soil Aeration ◽

Positive Effects ◽

High Level ◽

Different Growth Stages

Abstract Sensitivity to low rhizosphere soil aeration may change over time and therefore plant response may also depend on different growth stages of a crop. This study quantified effects of soil aeration during 5 different periods, on growth and yield of trickle-irrigated potted single tomato plants. Irrigation levels were 0.6 to 0.7 (low level) or 0.7 to 0.8 (high level) of total water holding capacity of the pots. Soil was aerated by injecting 2.5 l of air into each pot through the drip tubing immediately after irrigation. Fresh fruit yield, above ground plant dry weight, plant height, and leaf area index response to these treatments were measured. For all these 4 response variables, means of post-infiltration aeration between 58 to 85 days after sowing were 13.4, 43.5, 13.7, and 37.7% higher than those for the non-aerated pots, respectively. The results indicated that: post-infiltration soil aeration can positively impact the yield and growth of sub-surface trickle-irrigated potted tomato plants; positive effects on plant growth can be obtained with aeration during the whole growth period or with aeration for partial periods; positive growth effects of partial periods of aeration appears to persist and result in yield benefit.

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MORPHOLOGICAL AND BIOCHEMICAL RESPOSES OF COW PEA (CV. PUSA BARSATI) GROWN ON FLY ASH AMENDED SOIL IN PRESENCE AND ABSENCE OF MELOIDOGYNE JAVANICA AND RHIZOBIUM LEGUMINOSARUM

Ecoprint An International Journal of Ecology ◽

10.3126/eco.v17i0.4098 ◽

1970 ◽

Vol 17 ◽

pp. 17-22 ◽

Cited By ~ 1

Author(s):

Kamal Singh ◽

A. A. Khan ◽

Iram Khan ◽

Rose Rizvi ◽

M. Saquib

Keyword(s):

Fly Ash ◽

Plant Growth ◽

Rhizobium Leguminosarum ◽

Growth Yield ◽

Maximum Growth ◽

Meloidogyne Javanica ◽

Growth And Yield ◽

Negative Effects ◽

Positive Effects ◽

Insignificant Difference

Plant growth, yield, pigment and protein content of cow-pea were increased significantly at lower levels (20 and 40%) of fly ash but reverse was true at higher levels (80 and 100%). Soil amended by 60% fly ash could cause suppression in growth and yield in respect to 40% fly ash treated cow-pea plants but former was found at par with control (fly ash untreated plants). Maximum growth occurred in plants grown in soil amended with 40% fly ash. Nitrogen content of cow-pea was suppressed progressively in increasing levels of fly ash. Moreover, Rhizobium leguminosarum influenced the growth and yield positively but Meloidogyne javanica caused opposite effects particularly at 20 and 40% fly ash levels. The positive effects of R. leguminosarum were marked by M. javanica at initial levels. However, at 80 and 100% fly ash levels, the positive and negative effects of R. leguminosarum and/or M. javanica did not appear as insignificant difference persist among such treatments.Key words: Meloidogyne javanica; Rhizobium leguminosarum; Fly ash; Growth; YieldDOI: 10.3126/eco.v17i0.4098Ecoprint An International Journal of Ecology Vol. 17, 2010 Page: 17-22 Uploaded date: 28 December, 2010

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