scholarly journals Fasting ghrelin but not PYY(3-36) is associated with insulin-resistance independently of body weight in Wistar rats

2014 ◽  
Vol 58 (4) ◽  
pp. 377-381 ◽  
Author(s):  
Luciana da Conceição Antunes ◽  
Manoela Neves da Jornada ◽  
Jessica Lorenzzi Elkfury ◽  
Kelly Carraro Foletto ◽  
Marcello Casaccia Bertoluci
Keyword(s):  
Insulin Resistance ◽  
Body Weight ◽  
Multiple Regression ◽  
Wistar Rats ◽  
Serum Levels ◽  
Dark Cycle ◽  
Insulin Resistant ◽  
Male Wistar Rats ◽  
Overnight Fasting ◽  
Ad Libitum

Objective: The objective of this study was to evaluate the association between insulin-resistance and fasting levels of ghrelin and PYY in Wistar rats. Materials and methods: A total of 25 male Wistar rats, weighing 200-300 g, was included in this study. The animals were maintained in cages with a 12/12h light-dark cycle and fed standard chow and water ad libitum. After 12-h overnight fasting, ghrelin, PYY, insulin and glucose values were determined. Insulin resistance was assessed by means of the HOMA-IR, which was ranked and the median was used as a cut-off value to categorize insulin-resistance. HOMA-IR values equal and above 2.62 were considered insulin-resistant (IR) while values below 2.62 were considered insulin sensitive (IS). Differences between means were determined using the Student t-test. Multiple regression and Pearson’s correlation test were used to evaluate the association between variables. Results: HOMA-IR median IQ range values for IS and IR groups were, respectively, 1.56 (0.89 – 2.16) vs. [4.06 (3.50 – 4.61); p < 0.001]. The IR group presented increased levels of fasting ghrelin, PYY and insulin respectively: [50.35 (25.99 – 74.71) pg/mL vs. 12.33 (8.77 – 15.89) pg/mL; p = 0.001]; [54.38 (37.50 – 71.26) pg/mL vs. 33.17 (22.34 – 43.99) pg/mL; p = 0.016]; [18.04 (14.48 – 21.60) uU/mL vs. 7.09 (4.83 – 9.35) uU/mL; p = 0.001]. Ghrelin, but not PYY, correlated linearly and positively with HOMA-IR: ghrelin vs. HOMA-IR (r = 0.52; p = 0.008), and PYY vs. HOMA-IR (r = 0.22; p = 0.200). This correlation was independent of body weight. Conclusion: Fasting ghrelin and PYY serum levels are increased in lean, relatively insulin resistant Wistar rats, and this increase is independent of weight.

scholarly journals Effect of 4-h time restricted feeding on body weight, leptin concentration and lipid profile in healthy non-obese male Wistar rats

2021 ◽  
Author(s):  
Michael A. Olamoyegun ◽  
Folasade O. Ajao ◽  
Marcus O. Iyedupe
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Lipid Profile ◽  
Wistar Rats ◽  
Metabolic Diseases ◽  
Restricted Feeding ◽  
Mean Values ◽  
Male Wistar Rats ◽  
Ad Libitum ◽  
Obese Male

Abstract Background: Obesity greatly increases the risk of metabolic diseases and preventive approaches for obesity are often inadequate to effectively prevent and manage the diseases. Altering feeding time strategy intervention decreases caloric intake without calorie counting and may be an effective therapy. Therefore, this study investigates the effect of 4-h time restricted feeding on body weight, leptin concentration and lipid profile in healthy non-obese male Wistar rats. Methods: Rats placed on time-restricted feeding (TRF) regimen had freely access to food for 4 hour per day at designated periods. Twenty four rats divided into four groups (n=6) were used. Group I animals were placed on a 4 hour per day TRF between 8am-12noon. Group II rats were also placed on a 4 hour per day TRF between 12noon-4pm. Group III rats also placed on a 4 hour per day TRF between 8pm-12 midnight while Group IV rats had access food and water ad libitum. This diet strategy resembles taking only breakfast, lunch or dinner once a day. The study lasted for a period of 4 weeks with daily food intake and weekly body weight determined throughout the period. At the end of the experimental period, blood glucose, lipid profile and leptin concentration were assessed. SPSS 21.0 package was used for data analysis, one-way analysis of variance (ANOVA) was used to compare the mean values of variables among the groups and bonferroni’s posthoc test was used for significance of pair wise comparisons of mean values among the groups. Significance was set at p < 0.05.Results: In this study, the body weights and leptin concentrations of 8pm – 12am and ad libitum groups significantly increased compared with the 8am - 12noon and 12noon -4pm groups. Dyslipidemia was observed in the ad libitum group when compared with the 8am - 12noon and 12noon - 4pm groups. Conclusion: From this study, 4-hr time restricted feeding has beneficial effects on body weight, blood glucose, lipid profile and leptin concentration. This feeding restriction patterns may be helpful in obesity management and in preventing metabolic diseases development in non obese.

The Effects of Food Deprivation of Rats On Swimming To Exhaustion

Behaviour ◽  
1966 ◽  
Vol 26 (3-4) ◽  
pp. 351-356 ◽  
Author(s):  
Edward T. Uyeno ◽  
R. Alan Graham
Keyword(s):  
Body Weight ◽  
Food Deprivation ◽  
Wistar Rats ◽  
Control Group ◽  
Dorsal Skin ◽  
The Third ◽  
Male Wistar Rats ◽  
Ad Libitum ◽  
Fourth Experiment ◽  
Ad Libitum Feeding

AbstractMale Wistar rats were deprived of food for 3 days and then forced to swim continuously to a criterion of exhaustion. Controls swam after ad libitum feeding. In the first experiment a load equal to 11% of the rat's body weight was attached to the dorsal skin near the tail during the swim. In a second study, two matched groups, treated identically as those in the first study swam without a load in water treated with a wetting agent, "Aerosol O.T." In a third experiment, controls as well as experimentals were deprived of food for three days. The control group, however, was fed for 30 minutes, immediately before the swim. A fourth experiment was conducted in a similar manner as the third, except both groups were deprived 6 hours. In each of the experiments the deprived groups swam significantly longer than the fed groups.

scholarly journals Total gastrectomy with substitution of stomach by jejunal pouch with and without duodenal passage: study in rats

2005 ◽  
Vol 20 (suppl 1) ◽  
pp. 107-112 ◽  
Author(s):  
Tertuliano Aires Neto ◽  
Jeancarlo Fernandes Cavalcante ◽  
José Brandão-Neto ◽  
Irami Araújo Filho ◽  
Maria das Graças Almeida ◽  
...  
Keyword(s):  
Body Weight ◽  
Total Gastrectomy ◽  
Wistar Rats ◽  
Serum Gastrin ◽  
Serum Levels ◽  
Sham Operation ◽  
Jejunal Pouch ◽  
Total Proteins ◽  
Male Wistar Rats ◽  
Iron And Calcium

PURPOSE: A comparison was done between the F. Paulino jejunal pouch (FP) and a jejunal pouch (JP) as esophagus-duodenum interpositional graft, for replacing the stomach after total gastrectomy. It was investigated the effect of the two procedures on esophagus histology, nutritional state and serum gastrin in rats. METHODS: Male Wistar rats weighing 282±17g were randomly submitted to sham operation (S), FP and JP after total gastrectomy. After eight weeks the rats were killed with overdose of anesthetic and tissue was taken from the distal esophagus for histology. Serum levels of total proteins, albumin, iron, transferring, folate, cobalamine, calcium, as well as serum gastrin were determined. Survival was considered. RESULTS: Fourty six rats were operated and thirty survived for eight weeks. Five (33.3%) died after FP and 11 (52.3%) after JP (p<0.05). Postoperative esophagitis occurred in 6 JP rats. At 8th week, no difference was observed on body weight when compared FP and JP rats (p>0.05). The JP rats had a significant decrease in serum albumin, glucose, transferrin, iron, folate and calcium, compared to sham (p<0.05). Serum gastrin, iron and calcium were significantly higher in JP rats than in FP rats (p<0.05). In FP rats, transferrin and cobalamine showed significant decrease comparing the preoperative with 8th week levels (p<0.05). CONCLUSION: F. Paulino pouch in rats had lower mortality than JP, and esophagitis was not detected in it. JP rats had serum gastrin, iron and calcium unaffected, possibly because of preservation of duodenal passage.

Intermittent exposure to green and white light-at-night activates hepatic glycogenolytic and gluconeogenetic activities in male Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Abayomi O. Ige ◽  
Olubori S. Adekanye ◽  
Elsie O. Adewoye
Keyword(s):  
Insulin Resistance ◽  
Body Weight ◽  
Blood Glucose ◽  
Wistar Rats ◽  
Light At Night ◽  
Group Iii ◽  
Group I ◽  
Intermittent Exposure ◽  
Male Wistar Rats ◽  
Group Ii

Abstract Objectives Exposure to light-at-night (LAN) has been reported to impair blood glucose regulation. The liver modulates blood glucose through mechanisms influenced by several factors that include peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1α) and glucose-6-phosphatase (G6Pase). This study investigated the effect of intermittent exposure to green and white LAN on some hepatic glucose regulatory factors in male Wistar rats. Methods Animals were divided into three equal groups. Group I (control) was exposed to normal housing conditions. Groups II and III were each daily exposed to either green or white LAN for 2 h (7–9 pm) for 14 days. Body weight and blood glucose was monitored on days 0, 7, and 14. Thereafter, retro-orbital sinus blood was obtained after light thiopental anaesthesia and serum insulin was determined. Liver samples were also obtained and evaluated for glycogen, PGC-1α, and G6Pase activity. Insulin resistance was estimated using the HOMA-IR equation. Results Body weight and blood glucose on days 7 and 14 increased in groups II and III compared to control. Hepatic PGC-1α and G6Pase increased in group II (2.33 ± 0.31; 2.07 ± 0.22) and III (2.31 ± 0.20; 0.98 ± 0.23) compared to control (1.73 ± 0.21; 0.47 ± 0.11). Hepatic glycogen was 71.8 and 82.4% reduced in groups II and III compared to control. Insulin in group II increased (63.6%) whiles group III values reduced (27.3%) compared to control. Insulin resistance increased in group II (0.29 ± 0.09) compared to control (0.12 ± 0.03) and group III (0.11 ± 0.03), respectively. Conclusions Exposure to 2 h green and white LAN in the early dark phase increases hepatic glycogenolysis and gluconeogenetic activities resulting in increased blood glucose. In male Wistar rats, exposure to green but not white LAN may predispose to insulin resistance.

Qualitative regional differences in adipose tissue growth and cellularity in male Wistar rats fed ad libitum

1998 ◽  
Vol 274 (5) ◽  
pp. R1460-R1467 ◽  
Author(s):  
M. DiGirolamo ◽  
J. B. Fine ◽  
K. Tagra ◽  
R. Rossmanith
Keyword(s):  
Body Weight ◽  
Adipose Tissue ◽  
Regional Differences ◽  
Wistar Rats ◽  
Cell Number ◽  
Tissue Growth ◽  
Fat Cell ◽  
Fat Depots ◽  
Male Wistar Rats ◽  
Ad Libitum

Adipose tissue grows primarily by a combination of increases in fat cell volume (hypertrophy) and in fat cell number (hyperplasia), but the regional growth pattern of white adipose tissue depots in animal species and in the human is still unclear. In this study we characterized fully the age-related changes in adipose tissue growth, composition, and cellularity of four fat depots of male Wistar rats that varied in age from 7 wk to 15 mo and in body weight from 178 to 808 g. Body weight and the weight of each of the four adipose depots studied (epididymal, mesenteric, subcutaneous inguinal, and retroperitoneal) increased progressively with age and ad libitum feeding. Comparison of the cellularity of the four adipose depots, however, showed remarkable and significant differences in the pattern of growth within the same animals. The cumulative growth of the two intra-abdominal fat depots (mesenteric and epididymal) was due mostly to hypertrophy (increases in cell volume of 83 and 64%, respectively), whereas the growth of the other two depots (retroperitoneal and inguinal) was due predominantly to hyperplasia (increases in cell number of 58 and 65%, respectively). These findings uncover major and unexpected regional differences in the modulation of adipose tissue growth within aging animals fed ad libitum and suggest local, region-specific regulatory controls of this growth.

scholarly journals Tinospora crispaAmeliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Mohd Nazri Abu ◽  
Suhana Samat ◽  
Norathirah Kamarapani ◽  
Fuzina Nor Hussein ◽  
Wan Iryani Wan Ismail ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Serum Levels ◽  
The Body ◽  
High Fat ◽  
Normal Range ◽  
C Peptide ◽  
Male Wistar Rats ◽  
Histological Results

The antidiabetic properties ofTinospora crispa, a local herb that has been used in traditional Malay medicine and rich in antioxidant, were explored based on obesity-linked insulin resistance condition. Male Wistar rats were randomly divided into four groups, namely, the normal control (NC) which received standard rodent diet, the high fat diet (HFD) which received high fat diet only, the high fat diet treated withT. crispa(HFDTC), and the high fat diet treated with orlistat (HFDO). After sixteen weeks of treatment, blood and organs were harvested for analyses. Results showed thatT. crispasignificantly (p< 0.05) reduced the body weight (41.14 ± 1.40%), adiposity index serum levels (4.910 ± 0.80%), aspartate aminotransferase (AST: 161 ± 4.71 U/L), alanine aminotransferase (ALT: 100.95 ± 3.10 U/L), total cholesterol (TC: 18.55 ± 0.26 mmol/L), triglycerides (TG: 3.70 ± 0.11 mmol/L), blood glucose (8.50 ± 0.30 mmo/L), resistin (0.74 ± 0.20 ng/mL), and leptin (17.428 ± 1.50 ng/mL) hormones in HFDTC group. The insulin (1.65 ± 0.07 pg/mL) and C-peptide (136.48 pmol/L) hormones were slightly decreased but within normal range. The histological results showed unharmed and intact liver tissues in HFDTC group. As a conclusion,T. crispaameliorates insulin resistance-associated with obesity in Wistar rats fed with high fat diet.

Dose-dependent and time-dependent metabolic, hemodynamic, and redox disturbances in dexamethasone-treated Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Elvine P. Nguelefack-Mbuyo ◽  
Fernande P. Peyembouo ◽  
Christian K. Fofié ◽  
Télesphore B. Nguelefack
Keyword(s):  
Insulin Resistance ◽  
Body Weight ◽  
Catalase Activity ◽  
Wistar Rats ◽  
Chronic Treatment ◽  
Body Weight Loss ◽  
Redox Status ◽  
Duration Of Treatment ◽  
Renal Hypertrophy ◽  
Subchronic Treatment

Abstract Objectives Dexamethasone is used experimentally to induce insulin resistance and type 2 diabetes. However, data concerning the dose, the duration of treatment, and the associated comorbidities are inconsistent. The aim of this study was to compare the effects of different doses of dexamethasone and the duration of treatment necessary for the development of a model of insulin resistance that mimics the clinical condition with the associated comorbidities. Methods Dexamethasone was administered intramuscularly to male Wistar rats, at doses of 500 and 1,000 µg/kg/day for the subchronic treatment (eight consecutive days) and at doses of 5, 25, 50, and 100 µg/kg/day in chronic treatment (28 consecutive days). Effects on body weight, metabolism, hemodynamics, renal function, and redox status were evaluated. Results Both treatments induced a progressive body weight loss that was drastic in subchronic treatment, improved glucose tolerance without affecting fasting glycemia. Doses of 1,000 and 100 µg/kg were associated with hypertriglyceridemia, hypertension, and increased heart rate, cardiac and renal hypertrophy. Increased creatinemia associated with reduced creatinuria were observed in sub-chronic treatment while increased proteinuria and reduced creatinuria were noticed in chronic treatment. 1,000 µg/kg dexamethasone caused an increase in hepatic, and renal malondialdehyde (MDA) and glutathione (GSH) coupled with a reduction in catalase activity. The dose of 100 µg/kg induced a rise in GSH and catalase activity but reduced MDA levels in the kidney. Conclusions Doses of 1,000 µg/kg for subchronic and 100 µg/kg for chronic treatment exhibited similar effects and are the best doses to respective time frames to induce the model.

Ocimum gratissimum enhances insulin sensitivity in male Wistar rats with dexamethasone-induced insulin resistance

2021 ◽  
Author(s):  
Shehu-Tijani Toyin Shittu ◽  
Taye Jemilat Lasisi ◽  
Seyid Alli-Sisse Shittu ◽  
Adeyinka Adeyemi ◽  
Tolulope James Adeoye ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Insulin Sensitivity ◽  
Wistar Rats ◽  
Ocimum Gratissimum ◽  
Male Wistar Rats

scholarly journals Effects of proportional high-protein/low-carbohydrate formulated diet consumption in diabetic rats: Beneficial impact on glycemic and weight control

2020 ◽  
Vol 20 (07) ◽  
pp. 16984-16996
Author(s):  
MMC Anyakudo ◽  
◽  
DO Adeniji ◽  
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Treated Group ◽  
Morphological Features ◽  
High Protein ◽  
Control Group ◽  
Formulated Diet ◽  
Low Carbohydrate ◽  
Male Wistar Rats ◽  
And Control

The metabolic response to nutrient ingestion and the rate of digestion and absorption of nutrient molecules in bowel physiology plays an important role in the metabolic control of some human chronic non-infectious diseases. This experimentally-controlled designed nutritional study which lasted eight weeks aimed to determine the effects of proportional high-protein/low-carbohydrate (HP/LC) formulated diet on glycemic tolerance, glycemic control, body weight, organ weight and organ morphometry in healthy and diabetic adult male Wistar rats. Twenty-four male Wistar rats purchased from a disease-free stock were randomly categorized into four groups (n = 6, each) after two weeks acclimatization period in raised stainless steel cages with 6 mm2mesh floor and replaceable numbered blotters papers placed under each cage in a well-ventilated animal house. Animal groups include: Healthy control group (HC), Healthy treated group (HT), Diabetic control group (DC) and Diabetic treated group (DT. The animals were fed according to the experimental design with water ad libitumfor eight weeks. Diabetes was inducted with freshly prepared alloxan monohydrate solution (150 mg/kg bw, intraperitoneally). Body weights and fasting blood sugar concentrations were measured twice weekly, while oral glucose tolerance test was conducted on the last day of the eighth-week study and subsequently followed by organs extraction after anesthesia for weight and gross assessment. Proportional high-protein/low-carbohydrate formulated diet caused significant reduction in mean body weight of treated diabetic (DT: 22.6%; P= .001) and healthy (HT: 5.8%; P= .007) rats while the control animals on control diet recorded significant (P< .05) increase in body weight gain (DC: 12.4%; HC: 11.2%). Glycemic tolerance and control improved significantly in diabetic treated rats over that of the healthy treated rats. Gross morphometry of the extracted organs (kidneys, liver, heart, lungs, spleen and testes) revealed sustained normal morphological features without any visible lesion. In conclusion, consumption of proportional high-protein/low-carbohydrate formulated diet enhanced body weight reduction and sustained normal organ morphological features with good glycemic tolerance and control in experimental rats, suggesting its dietary potentiality, safety and suitability to ameliorate obesity-related diabetes.

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