LINEA ALBA COLLAGEN ASSESSMENT IN MORBIDLY OBESE PATIENTS

ABSTRACT Background: The evaluation of collagen in the abdominal wall has been increasingly studied because of the relevance on collagen in the healing process after laparotomy. Aim: To evaluate the amount of collagen in the linea alba of patients undergoing laparotomic bariatric surgery and comparing with non-obese cadavers. Methods: Were evaluated 88 samples of aponeurosis from abdominal linea alba of 44 obese patients (obesity group) and 44 non-obese cadavers (control group). The samples were collected in 2013 and 2104, and were sorted according to age (18-30, 31-45 and 46-60), gender, BMI, waist and cervical circumference, and subcutaneous tissue thickness. Material for biopsy was collected from the supraumbilical region of the linea alba for immunohistochemical analysis differentiating collagen type 1 and type 3 and the 1/3 ratio. Image-Pro Plus pixel counting software was used to measure the amount of collagen. Results: The obesity group evidenced mean age 44.11±9.90 years; 18-30 age group had three (6.8%) obese individuals; 31-45 had 22 (50%) and 46-60 had 19 (43.1%). Females were present in 81.8% (n=36); BMI (kg/m²) was 48.81±6.5; waist circumference (cm) was 136.761±13.55; subcutaneous tissue thickness (cm) 4.873±0.916. Considering age groups, gender and BMI, there were statistical differences in all tests when compared with the cadavers. Conclusion: The amount of collagen in the linea alba above the umbilical region in the morbidly obese patients was smaller than in the non-obese cadavers in the same age group.

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A comparative study between vacuum assisted wound closure vs conventional dressing in non-healing ulcers

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11ispl4.4444 ◽

2020 ◽

Vol 11 (SPL4) ◽

pp. 2214-2223

Author(s):

Ajay Rex R ◽

Balaji D ◽

Lakshmana R ◽

Gopi Ramu ◽

Reka

Keyword(s):

Hospital Stay ◽

Wound Closure ◽

Healing Process ◽

Control Group ◽

P Value ◽

Baseline Characteristic ◽

Age Group ◽

Average Hospital ◽

Study Results ◽

Study Population

In a surgical ward, acute and continual wounds have an effect on a minimal of 1% of the population. Vacuum-assisted wound closure (VAC) is a technique of Negative pressure in the wound to improve the healing process. To study the advantage of a vacuum assisted closure over conventional dressing in the management of chronic non-healing diabetic ulcers. To study the difference in the rate of amputation, hospital stays in case and control groups. Group1-case group – vacuum associated closure therapy. Group 2-Control group -conventional dressings. Most of the patients in the study population was in the age group of 41 -60 years. 82% of the study population was within the age group of 41-60 years. The two groups are comparable with their baseline characteristic of age, and the P-value is less than 0.05. Wounds were more common in males than females. Out of the 44 patients, 26 were male, i.e. 57% of the study population were males. About 68% of wounds occurred in the foot. About 50% of the culture showed staphylococcus. Nearly 27% of study participants had no growth. The hospital stay is less in VAC dressing when compared to the conventional dressings, who have an average hospital stay of 28 days and the relation is statistically significant (p-value<0.05). Mean hospital stay in Vacuum is 21 compared to stay of 28 in conventional dressings group. Patients in Vacuum had 12 SSG,9 discharge and 1 amputation. There is no statistically significant association in terms of grade of ulcer between the two groups(P =0.23). There is a statistically significant association between VAC and conventional in terms of the results of the Doppler study. (P<0.01). From the study results, it is obvious that VAC dressing has many advantages in terms of Low no of amputation, Earlier discharge, Minimal infection, Lesser complications, Healing in a better way.

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Oral Contraceptives Provide Protection against Anterior Cruciate Ligament Tears: A National Database Study of 165,748 Female Patients

Orthopaedic Journal of Sports Medicine ◽

10.1177/2325967119s00353 ◽

2019 ◽

Vol 7 (7_suppl5) ◽

pp. 2325967119S0035

Author(s):

Steven F. DeFroda ◽

Steven Louis Bokshan ◽

Samantha Worobey ◽

Lauren Ready ◽

Alan H. Daniels ◽

...

Keyword(s):

Clinical Evidence ◽

Cruciate Ligament ◽

Age Groups ◽

National Database ◽

Control Group ◽

Protective Effects ◽

Age Group ◽

Oral Contraceptive Pills ◽

Acl Tear ◽

Chi Squared

Objectives: ACL tears are more prevalent in females than males. One of the factors responsible for this may be the variation in levels of estrogen and progesterone. The purpose of this study was to query a large nationwide database to determine the potentially protective effects of oral contraceptive pills (OCPs) on ACL tears. We hypothesized that females taking OCPs would exhibit ACL tears at lower rates than a matched population of patients not taking OCPs. Methods: The PearlDiver database (www.pearldiverinc.com, Fort Wayne, IN) was queried for all OCP users amongst females aged 15-49. Female experiencing an ACL tear and undergoing surgery (CPT 29888) were compared to a control group of females undergoing surgery for an ACL tear, but not on OCPs. Patients were matched by age and Charlson comorbidity score. Chi-squared testing was used to assess for significant differences in the rate of ACL tears for the OCP and non-OCP users, according to age groups broken down into age intervals of 5 years. Results: There were a total 82,874 patients in both the OCP and non-OCP groups. There were a total of 569 (0.69%) ACL tears in the non-OCP group and 465 (0.56%) in the OCP group (p<0.001). In the non-OCP group, patients aged 15-19 accounted for 29.35% of all ACL tears, whereas, in the OCP group, this same age group only accounted for 13.33% of ACL tears. Among all age groups, the odds ratios for experiencing an ACL tear while on OCP was 0.82 (χ2=0.001, 95% CI 0.72-0.92) (Figure 1) compared to not using OCP. This protective effect was driven primarily by the 15-19 age group (odds ratio 0.37 (χ2<0.001, 95% CI 0.27-0.50)). Conclusion: This investigation utilizing a large national database found that usage of OCPs was associated with an 18% decrease in the risk of ACL tear when compared to a matched population of patients with ACL tears not taking OCPs. These findings were in line with smaller studies which demonstrated similar results. Additionally, it was shown that OCPs were most protective in the 15-19 year old age group, with a 63% reduction in tears in this group. Ongoing randomized trials in patients without ACL tear could help to demonstrate further clinical evidence for OCP usage as a way to modify risk factors for ACL tear. While clinical evidence is limited, there should be strong consideration for OCP usage in elite high school and college aged athletes, especially those who are at risk of ACL tear.

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Plasma and tissue pharmacokinetics of fosfomycin in morbidly obese and non-obese surgical patients: a controlled clinical trial

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/dkz203 ◽

2019 ◽

Vol 74 (8) ◽

pp. 2335-2340 ◽

Cited By ~ 3

Author(s):

Christoph Dorn ◽

David Petroff ◽

Nancy Neumann ◽

Alexander Kratzer ◽

Nahed El-Najjar ◽

...

Keyword(s):

Drug Exposure ◽

Peak Plasma ◽

Subcutaneous Tissue ◽

Plasma Concentrations ◽

Surgical Patients ◽

Pharmacokinetic Analysis ◽

Controlled Clinical Trial ◽

Morbidly Obese ◽

Obese Patients ◽

Tissue Pharmacokinetics

Abstract Objectives To assess the pharmacokinetics and tissue penetration of fosfomycin in obese and non-obese surgical patients. Methods Fifteen obese patients undergoing bariatric surgery and 15 non-obese patients undergoing major intra-abdominal surgery received an intravenous single short infusion of 8 g of fosfomycin. Fosfomycin concentrations were determined by LC-MS/MS in plasma and microdialysate from subcutaneous tissue up to 8 h after dosing. The pharmacokinetic analysis was performed in plasma and interstitial fluid (ISF) by non-compartmental methods. Results Thirteen obese patients (BMI 38–50 kg/m2) and 14 non-obese patients (BMI 0–29 kg/m2) were evaluable. The pharmacokinetics of fosfomycin in obese versus non-obese patients were characterized by lower peak plasma concentrations (468 ± 139 versus 594 ± 149 mg/L, P = 0.040) and higher V (24.4 ± 6.4 versus 19.0 ± 3.1 L, P = 0.010). The differences in AUC∞ were not significant (1275 ± 477 versus 1515 ± 352 mg·h/L, P = 0.16). The peak concentrations in subcutaneous tissue were reached rapidly and declined in parallel with the plasma concentrations. The drug exposure in tissue was nearly halved in obese compared with non-obese patients (AUC∞ 1052 ± 394 versus 1929 ± 725 mg·h/L, P = 0.0010). The tissue/plasma ratio (AUCISF/AUCplasma) was 0.86 ± 0.32 versus 1.27 ± 0.34 (P = 0.0047). Conclusions Whereas the pharmacokinetics of fosfomycin in plasma of surgical patients were only marginally different between obese and non-obese patients, the drug exposure in subcutaneous tissue was significantly lower in the obese patients.

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Placental Vascular Obstructive Lesions: Risk Factor for Developing Necrotizing Enterocolitis

Pathology Research International ◽

10.4061/2010/838917 ◽

2010 ◽

Vol 2010 ◽

pp. 1-7 ◽

Cited By ~ 3

Author(s):

Laure Dix ◽

Matthias Roth-Kleiner ◽

Maria-Chiara Osterheld

Keyword(s):

Risk Factor ◽

Preterm Infants ◽

Necrotizing Enterocolitis ◽

Premature Infants ◽

Strong Association ◽

Age Groups ◽

Control Group ◽

Age Group ◽

Significant Difference ◽

And Control

Necrotizing enterocolitis (NEC) is a severe neonatal disease affecting particularly preterm infants. Its exact pathogenesis still remains unknown. In this study, we have compared the prevalence of vascular obstructive lesions in placentae of premature newborns which developed NEC and of a control group. We further compared separately the findings of placentae of infants of less than 30 weeks of gestation, the age group in which NEC occurs most frequently. We found signs of fetal vascular obstructive lesions in 65% of the placentae of preterm patients developing NEC, compared to only 17% of the placentae of preterm patients in the control group. In the age groups below 30 weeks of gestation, 58.5% of placentae of later NEC patients presented such lesions compared to 24.5% in the control group. The significant difference between NEC and control group suggests a strong association between fetal vascular obstructive lesions and NEC. Therefore, we propose that fetal vascular obstructive lesions might be considered as a risk factor for the development of NEC in premature infants.

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Population Pharmacokinetics and Target Attainment of Ertapenem in Plasma and Tissue Assessed via Microdialysis in Morbidly Obese Patients after Laparoscopic Visceral Surgery

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00952-16 ◽

2016 ◽

Vol 61 (1) ◽

Cited By ~ 6

Author(s):

Mathias Wittau ◽

Stephan Paschke ◽

Max Kurlbaum ◽

Jan Scheele ◽

Neang S. Ly ◽

...

Keyword(s):

Population Pharmacokinetics ◽

Peritoneal Fluid ◽

Subcutaneous Tissue ◽

Plasma Concentrations ◽

Fat Free Mass ◽

Morbidly Obese ◽

Population Pharmacokinetic ◽

Obese Patients ◽

Target Attainment ◽

Tissue Penetration

ABSTRACT Ertapenem provides broad-spectrum activity against many pathogens, and its use is relevant for the prophylaxis and treatment of infections in morbidly obese patients undergoing surgery. However, its pharmacokinetics and tissue penetration in these patients are not well defined. We assessed the population pharmacokinetics and target attainment for ertapenem in the plasma, subcutaneous tissue, and peritoneal fluid of morbidly obese patients. Six female patients (body mass index, 43.7 to 55.9 kg/m2) received 1,000 mg ertapenem as 15-min infusions at 0 and 26 h. On day 2, the unbound ertapenem concentrations in plasma, subcutaneous tissue, and peritoneal fluid were measured by microdialysis; total plasma concentrations were additionally quantified. The probability of attaining a target of an unbound ertapenem concentration above the MIC for at least 40% of the dosing interval was predicted via Monte Carlo simulations. The population pharmacokinetic model contained two disposition compartments and simultaneously described all concentrations. For unbound ertapenem, total clearance was 12.3 liters/h (coefficient of variation, 21.6% for between-patient variability) and the volume of distribution at steady state was 57.8 liters in patients with a 53-kg fat-free mass. The area under the concentration-time curve (AUC) for ertapenem was 49% lower in subcutaneous tissue and 25% lower in peritoneal fluid than the unbound AUC in plasma. Tissue penetration was rapid (equilibration half-life, <15 min) and was variable in subcutaneous tissue. Short-term ertapenem infusions (1,000 mg every 24 h) achieved robust (>90%) target attainment probabilities for MICs of up to 1 mg/liter in plasma, 0.25 to 0.5 mg/liter in subcutaneous tissue, and 0.5 mg/liter in peritoneal fluid. Ertapenem presents an attractive choice for many pathogens relevant to morbidly obese patients undergoing surgery. (This study has been registered at ClinicalTrials.gov under identifier NCT01407965.)

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Plasma Thymus and Activation-Regulated Chemokine (TARC) As Diagnostic Marker in Pediatric Hodgkin Lymphoma

Blood ◽

10.1182/blood-2018-99-111861 ◽

2018 ◽

Vol 132 (Supplement 1) ◽

pp. 5312-5312

Author(s):

Eline Zijtregtop ◽

Waichu Wong ◽

Friederike Meyer- Wentrup ◽

Martha Lopez-Yurda ◽

Raoull Hoogendijk ◽

...

Keyword(s):

Atopic Dermatitis ◽

Hodgkin Lymphoma ◽

Sensitivity And Specificity ◽

Diagnostic Marker ◽

Age Groups ◽

Serum Levels ◽

Control Group ◽

Age Group ◽

Blood Biomarkers ◽

Serum Samples

Abstract Background Pediatric classical Hodgkin lymphoma (cHL) is a clonal disorder in an inflammatory background, also known as the microenvironment. This microenvironment is of major importance for growth and survival of the malignant Hodgkin/Reed Sternberg (HRS) cells. HRS cells and the microenvironment communicate through chemo- and cytokines. Blood biomarkers result from this active crosstalk, and may be a surrogate for lymphoma viability (Steidl et al, JCC 2011). Blood biomarkers are important because they hold the promise to be easily available and cost-effective. One promising biomarker in adult patients with cHL is the "Thymus and Activation-Regulated Chemokine, TARC (Plattell et al, Haematologica 2012). Elevated TARC levels are also described in patients with atopic dermatitis (Hijnen et al, J All Clin Immunol 2004). In adult cHL patients about 85% of patients have significantly elevated levels of TARC in pre-treatment serum or plasma compared to healthy controls (Plattell et al). So far nothing is known about TARC in pediatric cHL patients. To define its value as a diagnostic marker in pediatric cHL patients, we compared TARC levels of pediatric cHL patients with control patients. This study was IRB-approved and registered under Dutch Trial registry number 6876. Methods After providing informed consent, plasma and serum samples were collected of newly diagnosed cHL patients before start of treatment. To define normal values of TARC in children, samples were collected from non-cHL randomly selected patients from the hematology, endocrinology and oncology outpatient clinic. Baseline characteristics including history of atopic dermatitis were collected. These control patients were divided in three age groups (0-9,10-14 and 15-18 years). TARC levels were measured by enzyme-linked immunosorbent assay (R&D systems, Human CCL17/TARC Quantikine ELISA Kit). TARC levels of the cHL patients were compared to the control group to obtain ROC curves and calculate the AUC, cross-validated sensitivity and specificity and accuracy of TARC as a diagnostic marker. We hypothesized that pediatric cHL patients had elevated pretreatment TARC levels in both serum and plasma. Analyses were done using SAS V9.4. Results Fourteen cHL patients were included with a median age of 14 (range 11-17) years. Ten (71.4%) were female. Eighty patients were included in the control group with a median age of 12 (range 10 months-18) years. Twenty-nine patients (36.3%) were included in age group 0-9, 25 (31.2%) in age group 10-14 and 26 (32.5%) in age group 15-18. Thirty-nine (48.8%) were female. Patients of the control group had a median TARC value of 71 (range 18-762) pg/ml for plasma and 318 (range 27-1300) pg/ml for serum. TARC plasma and serum levels decreased with age (Spearman correlation -0.26, 2-tailed p=0.0204), but there were no statistically significant pairwise comparisons found between the pre-specified age groups. In the eight control patients (10%) with atopic dermatitis no significantly higher plasma and serum levels were found (plasma median with eczema 97 versus 70 pg/ml without eczema (p=0.71) and serum median with eczema 643 versus 317 pg/ml (p=0.71)). Plasma was collected in 14 cHL patients, and all had elevated TARC levels, with a median plasma level of 18449 (range 1635-55821) pg/mL. Serum samples were collected in 8/14 cHL patients and all had elevated serum TARC levels. Median serum level: 46703 (range 12817-149739) pg/ml. The plasma TARC levels of cHL patients were significantly higher than those of the control group patients (p<0.001). With a cut-off of level of of 898.70 pg/ml, we obtained 100% (95% CI 73% - 100%) sensitivity and 100% (95% 94% - 100%) specificity. Serum TARC levels also were significantly higher than those of the control group patients (p<0.001), with a cut-off level of 10283.57 pg/ml, sensitivity and specificity will be 100% (95% CI 60% - 100% for sensitivity and 95% CI 94% - 100% for specificity). Conclusion All classical cHL patients had significantly higher TARC levels compared to the 80 control patients. Despite the small sample size of cHL patients, TARC was found to be a sensitive and specific diagnostic marker for pediatric cHL in both plasma and serum. Further research with a bigger sample of cHL patients is necessary to improve the accuracy of the sensitivity, as well as to investigate whether TARC is also a valuable marker for disease response during treatment in pediatric patients with cHL. Disclosures No relevant conflicts of interest to declare.

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Study on Psychiatric Evaluation of Morbidly Obese Patients

International Neuropsychiatric Disease Journal ◽

10.9734/indj/2021/v16i230169 ◽

2021 ◽

pp. 8-16

Author(s):

Asmaa Reda Elsayed Elshazly ◽

Mohammad Abdelhakeem Seleem ◽

Mohamed Hamdy Abo-Ryia ◽

Adel Abdel-Kareem Badawy

Keyword(s):

Mental Health ◽

Body Mass Index ◽

Morbid Obesity ◽

Binge Eating ◽

Psychiatric Disorders ◽

Body Mass ◽

Eating Attitude ◽

Control Group ◽

Morbidly Obese ◽

Obese Patients

Background: Obesity is becoming an important issue for health promotion. The World Health Organization estimated that around 1.5 billion adults were overweight (body mass index (BMI) 25 kg/m2) and about 500 million people were obese (BMI 30 kg/m2) in 2008. The relationship between obesity and mental health is also considered important. In a community-based study, obesity was positively associated with several mental disorders, especially mood disorders and anxiety disorders. The aim of the study is the assessment of current and lifetime psychiatric disorders among morbidly obese patients. Methods: This case control study was conducted on 60 participants from outpatient clinic of GIT surgery unit and community. All participants were subjected to: Body weight and body mass index, Psychiatric interview for diagnosis of psychiatric disorders by Arabic version of MINI, Scale for diagnosis of Bulimia nervosa by Shokeer, Scale for diagnosis of Anorexia Nervosa by Shokeer, Binge Eating Disorder Screener-7, Eating attitude test, Hamilton Depressions Rating Scale and Hamilton anxiety scale. Results: There was a significant increase in anxiety in patients with morbid obesity compared to control group. There was a significant difference between both groups showing the high prevalence of depression in patients with morbid obesity. Based on EAT test, there was a significant prevalence of abnormal eating behaviors in patients group compared to none of control group. A screening test for the presence of Binge eating symptoms revealed significant increase of symptoms in patients’ group. Conclusions: Psychiatric disorders are prevalent in morbidly obese patients and are associated with significantly worse quality of life. In addition, morbidly obese patients had significantly worse physical and mental health relative to control group from general population. High rates of psychiatric disorders among morbidly obese patients seem to be the rule rather than an exception.

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Mode of use by mobile phone and change of time of simple audio-motor reaction for users of mobile communication. Age related features of ipsilateral and contralateral effects

Biomedical Radioelectronics ◽

10.18127/j15604136-202101-05 ◽

2021 ◽

Author(s):

N.I. Khorseva ◽

O.R. Al’-Kudri ◽

P.E. Grigoryev ◽

R.I. Islyamov ◽

N.Yu. Shulzhenko

Keyword(s):

Electromagnetic Radiation ◽

Mobile Phone ◽

Auditory System ◽

Age Groups ◽

Control Group ◽

Age Group ◽

Motor Reaction ◽

Age Related ◽

Contralateral Effect ◽

Contralateral Effects

By analysing the time of a simple auditory-motor reaction with mono-presentation of an audio signal, the effect of the electromagnetic radiation of a mobile phone on the human auditory system is evaluated. Regularities in the frequency of occurrence of ipsi and contralateral effects in mobile users of different age groups are obtained. This study has no analogues in either Russian or foreign publications and is currently exclusive. The purpose was to study age-related features of the severity of ipsi and contralateral effects, depending on the mode of use of the mobile phone. To record the ipsi- and contralateral effects, we used the differences in the average values of a simple auditory-motor reaction between the left and right ears with mono-presentation of the sound signal of 573 respondents of different age groups. The obtained values were compared with the control group data for each age group (491 respondents). If the difference was greater than in the control group, the ipsilateral or contralateral effect was recorded. The severity of the effect was evaluated taking into account the lateral preferences of each participant in the experiments (to which ear the mobile phone is most often held) and the mode of use of the mobile phone. Statistical processing of the results was carried out using the non-parametric Mann-Whitney test and the method of descriptive statistics: prevalence indicator (Pr) (relative frequency of manifestation of a particular attribute in the sample). The comparison of the severity of ipsi and contralateral effects for different age groups allowed us to establish the following patterns. It was found that, in general, the manifestation of the contralateral effect decreases with age, and the ipsilateral effect increases. However, this change is not linear. It is shown that the features of the manifestation of the contralateral and ipsilateral effect for each age group depend both on the time of daily use of a mobile phone and on the total duration of use of a mobile phone. We believe that when studying multidimensional input data, an individual approach is necessary. The presented results confirm the negative effect of the radiation of mobile phones on the auditory system, primarily of our younger generation. Given the special vulnerability of children to physical environmental factors and the depth of penetration of EMR MT into the brain of a child, we believe that from a radiobiological point of view, there is already a need to develop a special SanPiN (sanitary rules and norms) for all available modern low-intensity sources of electromagnetic radiation, including Wi-Fi.

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Outcomes of Bariatric Surgery in Morbidly Obese Patients with Multiple Sclerosis

Journal of Obesity ◽

10.1155/2017/1935204 ◽

2017 ◽

Vol 2017 ◽

pp. 1-5 ◽

Cited By ~ 3

Author(s):

Kalman Bencsath ◽

Adham Jammoul ◽

Ali Aminian ◽

Hideharu Shimizu ◽

Carolyn J. Fisher ◽

...

Keyword(s):

Multiple Sclerosis ◽

Bariatric Surgery ◽

Weight Loss ◽

Excess Weight ◽

Control Group ◽

Morbidly Obese ◽

Obese Patients ◽

Percent Excess Weight Loss ◽

Severely Obese

Obesity is common in patients with multiple sclerosis (MS); however, safety and efficacy of bariatric surgery in this population remain unclear. A database of 2,918 was retrospectively reviewed, yielding 22 (0.75%) severely obese patients with MS who underwent bariatric surgery. Sixteen surgical patients with complete follow-up data were matched to a nonsurgical control group of MS patients, based on age, BMI, MS subtype, and length of follow-up. MS relapse rates and trends in the timed twenty-five foot walk test (T25FW) were compared. In the surgical group (gastric bypass n=19, sleeve gastrectomy n=3), preoperative BMI was 46.5 ± 7.2 Kg/m2 and average excess weight was 60.4 kg. Follow-up data was collected at 59.0 ± 29.8 months. There were two major and four minor complications. Five patients required readmission and there were no mortalities. Percent excess weight loss was 75.5 ± 27.0%. In the 16 patients with follow-up data, patients who underwent bariatric surgery were significantly faster on the T25FW compared to the nonsurgical population. In conclusion, bariatric surgery is relatively safe and effective in achieving weight loss in patients with MS. In addition, surgery may help patients maintain ambulation. Findings support the need for further studies on bariatric surgery and disease-specific outcomes in this population.

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Effect of Substrate Age and Adhesive Composition on Dentin Bonding

Operative Dentistry ◽

10.2341/12-307-l ◽

2013 ◽

Vol 38 (3) ◽

pp. 267-274 ◽

Cited By ~ 22

Author(s):

J Perdigão ◽

Ana Sezinando ◽

Paulo C Monteiro

Keyword(s):

Age Groups ◽

Control Group ◽

Age Group ◽

Single Bond ◽

Human Teeth ◽

Adhesive Composition ◽

Dentin Adhesives ◽

Significant Difference ◽

Post Hoc ◽

Substrate Age

SUMMARY Purpose To study the effect of dentin age and adhesive composition on the microtensile dentin bond strengths (μTBS) of five dentin adhesives. Materials and Methods Sixty extracted caries-free human teeth were assigned to the appropriate age group: less than 21 years of age (<21), 21–40 years of age (21–40), and greater than 40 years of age (>40). For each age group, specimens were randomly divided into five dentin adhesives: (1) Adper Easy Bond (EB, 3M ESPE), a one-step self-etch adhesive; (2) Experimental Adper Easy Bond without the Vitrebond Co-polymer (CP) (EBnoCP, 3M ESPE); (3) Adper Single Bond Plus (SB, 3M ESPE), a two-step etch&rinse adhesive; (4) Experimental Adper Single Bond Plus without CP (SBnoCP, 3M ESPE); and (5) Adper Scotchbond Multi-Purpose (MP, 3M ESPE), a three-step etch&rinse adhesive, as the control group. Specimens were sectioned in X and Y directions and the resulting beams were tested to failure in tension mode at a crosshead speed of 1 mm/min. Statistical analysis was computed using t-test and two-way analysis of variance followed by Fisher least significant difference multiple comparison post hoc test at p < 0.05. Results The highest mean μTBS values were obtained in the control group (MP) for all age groups. EB resulted in statistically similar mean μTBS compared to EBnoCP for all age groups: p = 0.538 for (<21); p = 0.974 for (21–40); and p = 0.909 for (>40). SB resulted in statistically higher mean μTBS than SBnoCP for all age groups [p<0.009 for (<21); p<0.028 for (21–40); and p<0.041 for (>40)]. MP, the control group, resulted in statistically lower mean μTBS when applied to the oldest age group (>40) compared to the youngest age group (<21), at p < 0.04. When means were pooled for the variable ‘age group,' SB resulted in significantly higher mean μTBS than SBnoCP at p < 0.009, while EB resulted in statistically similar mean μTBS compared to EBnoCP (p=0.9). MP resulted in statistically higher mean μTBS than all other adhesives—SB (p<0.0001), SBnoCP (p<0.0001), EBnoCP (p<0.022), and EB (p<0.046). Conclusions The substrate age influenced the bonding ability of the three-step etch&rinse adhesive. The presence of a carboxylic-based polymer (CP) enhanced the bonding ability of the two-step etch&rinse adhesive.

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