scholarly journals Exogenous antioxidants on quality of cabbage seeds

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Danúbia Aparecida Costa Nobre ◽  
Adriene Aparecida Silva ◽  
Gisele Machado Fernandes ◽  
Geraldo Humberto Silva ◽  
Willian Rodrigues Macedo
Keyword(s):  
Superoxide Dismutase ◽  
Ascorbate Peroxidase ◽  
Defense Mechanisms ◽  
Kojic Acid ◽  
Control Treatment ◽  
Antioxidant Compounds ◽  
Beneficial Effects ◽  
Radicle Protrusion ◽  
Peroxidase Enzyme ◽  
Biochemical Analyses

Abstract During seed germination there is production of reactive oxygen species, which, in a controlled way, are important to cell signaling and protection against pathogens, but, in excess, impair germination. Therefore, the objective of this study was to assess the action of different compounds on antioxidant mechanisms and enzymatic activation in cabbage seeds. Compounds like kojic acid, thymol and tyrosol were used to imbibe the cabbage seeds together with distilled water, and a control treatment without imbibition was used as well, with subsequent assessment by means of germination test, endosperm rupture, vigor, radicle protrusion, and assessment of seedling biochemical analyses by the activity of enzymes ascorbate peroxidase, catalase, superoxide dismutase and α-amylase. Data were subjected to analysis of variance and to the LSD means comparison test. Seeds treated with tyrosol presented higher results on the rupture of the endosperm, germination and vigor, and root development increased with use antioxidants. For the activity of antioxidant enzymes in seedlings, only kojic acid showed increase in the superoxide dismutase activity. There was also a reduction in the catalase activity with the use of thymol and tyrosol compounds compared to dry-seed assessments. After tyrosol treatment, ascorbate peroxidase enzyme was not detected, and water-imbibed seeds showed higher α-amylase activity. The use of antioxidant compounds has beneficial effects on cabbage seeds, and soaking with tyrosol led to better physiological quality, with activation of antioxidant defense mechanisms during germination.

scholarly journals Evaluation of phytochemical and superoxide dismutase activities of Enhalus acoroides (L.f.) Royle from coastal waters of North Sulawesi, Indonesia

2020 ◽  
Vol 13 (4) ◽  
pp. 676-680
Author(s):  
Febry S. I. Menajang ◽  
Mohammad Mahmudi ◽  
Uun Yanuhar ◽  
Endang Yuli Herawati
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Natural Antioxidants ◽  
Antioxidant Compounds ◽  
Beneficial Effects ◽  
Enhalus Acoroides ◽  
North Sulawesi ◽  
Antioxidant Agents ◽  
Phytochemical Constituents ◽  
Concentration Series

Background and Aim: Seagrasses are an excellent and potential bioresource to discover new natural bioactive compounds such as antioxidants that have beneficial effects on health. Natural antioxidants have many functions in biological systems, primarily for defense against oxidation which produces free radicals in food, chemicals, and living systems. This study aimed to discover new natural antioxidant agents, Enhalus acoroides (L.f.) Royle was evaluated for phytochemical constituents and the antioxidant activity against superoxide dismutase (SOD) was assessed. Materials and Methods: Sample specimens of E. acoroides (L.f.) Royle collected from two different areas, Manembo- Nembo, Bitung (SG-A) and Bahoi, Likupang Barat (SG-B) waters, were extracted with methanol and solutions were prepared in a concentration series. Results: Extracts of the seagrass E. acoroides (L.f.) Royle cultivated in different areas have different phytochemical constituents and SOD activities. The secondary metabolites of phenols, flavonoids, and steroids contained in the ethyl acetic extracts of E. acoroides were linearly correlated with their antioxidant activity, which exhibited an IC50 of 7 ppm. Conclusion: E. acoroides (L.f.) Royle samples cultivated in the two areas contained different phytochemical constituent profiles, indicating an effect of environmental factors, and both can be used as potential natural sources of antioxidant compounds.

Protective enzymes against reactive oxygen species during ripening of tomato (Lycopersicon esculentum) fruits in response to low amounts of UV-C

2001 ◽  
Vol 28 (8) ◽  
pp. 785 ◽  
Author(s):  
Essaid Ait Barka
Keyword(s):  
Superoxide Dismutase ◽  
Lycopersicon Esculentum ◽  
Ascorbate Peroxidase ◽  
Defense Mechanisms ◽  
Phenylalanine Ammonia Lyase ◽  
Tomato Fruit ◽  
Ascorbate Oxidase ◽  
Guaiacol Peroxidase ◽  
Second Period ◽  
Uv C

In previous studies with tomato (Lycopersicon esculentum L.) exposed to a low level (3.7 kJ m –2) of UV-C (λ: 254 nm) radiation, which is defined as a beneficial level, we report a delay in fruit ripening by at least 1 week for treated fruit. In the present study, we investigate the changes in the activities of different enzymes involved in defense mechanisms, such as guaiacol peroxidase, ascorbate peroxidase, catalase, superoxide dismutase, ascorbate oxidase, lipoxygenase and phenylalanine ammonia lyase in tomato fruit in response to a beneficial level of UV-C. The irradiation leads to an increase in the guaiacol peroxidase and ascorbate peroxidase activities, whereas catalase activity remains similar to the control. The activities of superoxide dismutase and ascorbate oxidase were significantly reduced after UV-C exposure. In UV-C-treated fruit, an increase of lipoxygenase and phenylalanine ammonia lyase activities occurred within the first 5 d, followed by a second period in which these activities were below those of the control. Our study suggests that the level of UV-C used induced a rapid but moderate accumulation of photooxidation products, to which plants react by stimulating their defence mechanisms against oxidation. This activation may explain the delay observed in ripening and senescence of irradiated tomato fruit.

Different Carotenoid Enrichment in Two Climacteric Fruits after Post- Harvest UV-B Treatment

2020 ◽  
Vol 16 (2) ◽  
pp. 102-108
Author(s):  
Carolina F. Assumpção ◽  
Médelin M. da Silva ◽  
Vanessa S. Hermes ◽  
Annamaria Ranieri ◽  
Ester A. Ferreira ◽  
...  
Keyword(s):  
Bioactive Compound ◽  
Ultraviolet B ◽  
Low Flow ◽  
Control Treatment ◽  
Carotenoid Content ◽  
Post Harvest ◽  
Beneficial Effects ◽  
Fruit Skin ◽  
Compound Synthesis ◽  
Viable Method

Background: Ultraviolet B (UV-B) radiation is a promising and environmentally friendly technique, which in a low flow rate, can induce bioactive compound synthesis. This work aimed at evaluating the effectiveness of post-harvest UV-B treatment in order to improve carotenoid content in climacteric fruits like persimmon and guava fruits. Methods: The fruits were harvested at commercial maturity and placed into climatic chambers equipped with UV-B lamps. For control treatment, the UV-B lamps were covered by a benzophenone film, known to block the radiation. This radiation was applied during 48 hours and fruits were sampled at 25, 30 and 48 hours of each treatment. HPLC analysis was performed to separate and identify carotenoid compounds from fruit skin after a saponification process. Results: Fruit from 30 hours treatment began to present a carotenoid accumulation since the majority of analyzed compounds exhibited its synthesis stimulated from this time on. In persimmon skin, it was observed that the maximum content was reached after 48 hours of UV-B treatment. Conclusion: These results suggest that this post-harvest UV-B treatment can be an innovative and a viable method to induce beneficial effects on guava and mainly on persimmon fruit.

Modulation of longevity-associated genes by estrogens or phytoestrogens

2008 ◽  
Vol 389 (3) ◽  
pp. 273-277 ◽  
Author(s):  
Jose Viña ◽  
Juan Sastre ◽  
Federico V. Pallardó ◽  
Juan Gambini ◽  
Consuelo Borrás
Keyword(s):  
Superoxide Dismutase ◽  
Nuclear Factor Kappa B ◽  
Chemical Properties ◽  
Nuclear Factor ◽  
Antioxidant Protection ◽  
Beneficial Effects ◽  
Pathway Activation ◽  
Map Kinase Pathway ◽  
Kinase Pathway ◽  
Lower Peroxide

Abstract Females live longer than males. We have shown that the higher levels of estrogens in females protect them against aging, by up-regulating the expression of antioxidant, longevity-related genes, such as that of selenium-dependent glutathione peroxidase (GPx) and Mn-superoxide dismutase (Mn-SOD). Both estradiol and genistein (the most abundant phytoestrogen in soybeans) share chemical properties which confer antioxidant features to these compounds. However, the low concentration of estrogens and phytoestrogens make it unlikely that they exhibit significant antioxidant capacity in the organism. Physiological concentrations of estrogens and nutritionally relevant concentrations of genistein activate the MAP kinase pathway. These, in turn, activate the nuclear factor kappa B (NF-κB) signaling pathway. Activation of NF-κB by estrogens subsequently activates the expression of Mn-SOD and GPx, but genistein is only capable of activating Mn-SOD expression. This could be due to the fact that genistein binds preferably to estrogen receptor β. The antioxidant protection is reflected in the lower peroxide levels found in cells treated with estrogens or phytoestrogens when compared with controls. The challenge for the future is to find molecules that have the beneficial effects of estradiol, but without its feminizing effects. Phytoestrogens or phytoestrogen-related molecules may be good candidates to meet this challenge.

scholarly journals The beneficial effects of polyethylene glycol-superoxide dismutase on ovarian tissue culture and transplantation

2015 ◽  
Vol 32 (10) ◽  
pp. 1561-1569 ◽  
Author(s):  
Eun Jung Kim ◽  
Hee Jun Lee ◽  
Jaewang Lee ◽  
Hye Won Youm ◽  
Jung Ryeol Lee ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Superoxide Dismutase ◽  
Polyethylene Glycol ◽  
Ovarian Tissue ◽  
Beneficial Effects

scholarly journals Morphological and Biochemical Alterations of Paddy Rice in Response to Stress Caused by Herbicides and Total Plant Submersion

2017 ◽  
Vol 35 (0) ◽  
Author(s):  
M.G. MARCHEZAN ◽  
L.A. AVILA ◽  
D. AGOSTINETTO ◽  
C.E. SCHAEDLER ◽  
A.C. LANGARO ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Ascorbate Peroxidase ◽  
Paddy Rice ◽  
Total Phenolic ◽  
Total Chlorophyll ◽  
Water Regimes ◽  
Biochemical Alterations ◽  
Response To Stress ◽  
Total Plant ◽  
Induced Changes

ABSTRACT Herbicide selectivity in paddy rice varies in several aspects, among which are the environmental conditions. The aim of the study was to evaluate the effect of herbicide application and total plant submersion on morphological and biochemical changes in paddy rice. Total chlorophyll and carotenoids, catalase activity, ascorbate peroxidase and superoxide dismutase, total phenolic content, lipid peroxidation and hydrogen peroxide levels were assessed. Leaf samples were collected 24 hours and 7 days after the application of water regimes. The results observed in the first experiment show that cultivars Puitá INTA CL, IRGA 417 and IRGA 422 CL are more tolerant to total submersion. The most sensitive cultivars are IRGA 424, BRS Querência, EPAGRI 108 and BRS Taim. In general, cultivar Puitá INTA CL had lower oxidative damage than BRS Querência when under submersion. To eliminate excess free radicals, BRS Querência had increased activity of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) than Puitá INTA CL under submersion. Formulations with imazethapyr + imazapic and imazapyr + imazapic caused greater reduction in the total chlorophyll and carotenoid contents at 7 days after the establisment of water regimes (DAT). Therefore, the data show that exposing cultivars to total submersion and herbicides increased oxidative stress as well as induced changes in the activities of antioxidant enzymes.

scholarly journals Postharvest Quality of Cut Roses With the Application of Salicylic Acid

2018 ◽  
Vol 10 (8) ◽  
pp. 397
Author(s):  
Sérgio Miguel Mazaro ◽  
Edson Bertoldo ◽  
Nean Locatelli Dalacosta ◽  
Fabiana Chiamulera Borsatti ◽  
Mycheli Preuss da Cruz ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Defense Mechanisms ◽  
Phenylalanine Ammonia Lyase ◽  
Postharvest Quality ◽  
Fresh Matter ◽  
Leaf Chlorophyll ◽  
Biochemical Analyses ◽  
Stem Curvature ◽  
Lower Stem

The objective of this work was to evaluate the effect of the application of salicylic acid (SA) on the maintenance of quality and longevity of cut roses cv. Vega. Cut roses were kept in a vase solution of SA and water at concentrations of 0; 0.5; 1.0; 1.5 and 2.0 mM. All treatments were kept at 8±2 oC for 96 hours, simulating storage in flower shops; the flowers were then evaluated regarding loss of fresh matter and leaf chlorophyll content and were transferred to beakers containing distilled water at 25±2 ºC for more 144 hours, simulating shelf life. At 24, 48, 72 and 96 hours from the beginning of the experiment, biochemical analyses of total proteins and the activity of the phenylalanine ammonia-lyase (PAL) and peroxidases (PO) were performed. Visual analyses were performed (stem curvature, turgescence and petal darkening) every 48 hour intervals until the end of the experiment. The treatments with SA allowed the maintenance of post-harvest quality, reducing the loss of fresh matter mass, lower stem curvature, greater turgescence and less darkening of the petals. The results showed that the application of SA increased total protein contents and FAL, which characterizes the activation of plant defense mechanisms to the senescence process.

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