scholarly journals Alternative treatment to stimulate the germination of recently harvested wheat seeds

2016 ◽  
Vol 38 (4) ◽  
pp. 344-349
Author(s):  
Tereza Cristina de Carvalho ◽  
Osvaldo de Castro Ohlson ◽  
Maristela Panobianco
Keyword(s):  
Seed Dormancy ◽  
Alternative Treatment ◽  
Wheat Seed ◽  
Germination Test ◽  
Evaluation Period ◽  
Analytical Laboratories ◽  
Tetrazolium Test ◽  
Wheat Seeds ◽  
Operational Capacity

Abstract: The germination test for recently harvested wheat seeds requires a period of up to 20 days, because of the presence of dormancy. The possibility of reducing this period provides greater speed to the allocation of seeds, as well as an increased operational capacity of analytical laboratories. This study aimed at evaluating the effectiveness of treatments to stimulate the germination of recently harvested wheat seeds, reducing the five-day pre-cheeling period recommended to run the test. Seeds from various wheat genotypes were used: BRS Guamirim, BRS Tangará, BRS Pardela, CD 108, CD 116, CD 150, IPR Catuara TM, Frontana, IPR Taquari, Safira and Quartzo. They were analyzed immediately after collection and then stored for 30 and 180 days. In each evaluation period (immediately after harvesting, 30 and 180 days of storage), the determination of water content, the germination test (seeds without pre-chilling and pre-chilling between 5 and 10 ° C for three to five days), and also the tetrazolium test to quantify viability and seed dormancy were carried out. Based on these results, it is concluded that there is effectiveness in overcoming wheat seed dormancy, adopting the pre-cheeling procedure between 5 and 10 ° C for three days.

scholarly journals Tetrazolium test adjustment for wheat seeds

2013 ◽  
Vol 35 (3) ◽  
pp. 361-367 ◽  
Author(s):  
Tereza Cristina de Carvalho ◽  
Francisco Carlos Krzyzanowski ◽  
Osvaldo de Castro Ohlson ◽  
Maristela Panobianco
Keyword(s):  
Seed Viability ◽  
Longitudinal Section ◽  
Wheat Seed ◽  
Germination Test ◽  
Efficient Procedure ◽  
Paper Towels ◽  
Direct Immersion ◽  
Tetrazolium Test ◽  
Wheat Seeds ◽  
Test Adjustment

The assessment of the germination test in wheat seeds varies from 4 to 15 days, because the species normally presents dormancy in freshly harvested seeds. The tetrazolium test can characterize seed viability in less than 24 hours including lots with dormancy seeds. The objective of this study was to develop a practical and efficient procedure for evaluating the viability of wheat seeds using the tetrazolium test. Five seed lots of the BRS 208 cultivar were used, where the following were tested: a) pre-conditioning between moist paper towels or direct immersion in water for 18 hours, at 20 °C; b) longitudinal section of the embryo and the endosperm; c) coloration on paper or by immersion for 2 and 3 hours, at 30 and 40 °C; and d) concentrations of tetrazolium solution at 0.075%, 0.1%, 0.5% and 1.0%. The tetrazolium test may be efficiently used to evaluate wheat seed viability by pre-conditioning the seeds between paper towels (18 hours, at 20 °C) and adopting the following combinations of preparation and coloration: coloration of both halves of the seed on paper (2 hours, at 30 °C), in a 1.0% tetrazolium solution; or coloration of one half of the seed by immersion (3 hours, at 30 °C), in a 0.1% tetrazolium solution; or coloration of one half of the seed by immersion (2 hours, at 40 °C), in a 0.075% tetrazolium solution. This latter procedure is recommended for identifying and discarding lots with lower viability.

scholarly journals Improved assessment of wheat seeds vigor

2012 ◽  
Vol 36 (6) ◽  
pp. 608-614 ◽  
Author(s):  
Tereza Cristina de Carvalho ◽  
Francisco Carlos Krzyzanowski ◽  
Osvaldo de Castro Ohlson ◽  
Maristela Panobianco
Keyword(s):  
Moisture Content ◽  
Filter Paper ◽  
Seed Quality ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Wheat Seed ◽  
Efficient Procedure ◽  
Tetrazolium Test ◽  
Wheat Seeds

The tetrazolium test is widely used for seed vigor assessment of various plant species. For wheat, however, there is no methodology specifically recommended. This study aimed at determining an efficient procedure to evaluate vigor of wheat seeds by this test; besides allowing the establishment of seed quality classes. Thus, four different wheat seed lots were assessed by determining the moisture content and through tests of germination, accelerated aging, and field seedling emergence. The following methodologies were assessed: 1) longitudinal bisection of seed, with subsequent placement of the two halves on filter paper moistened with a 1.0% tetrazolium solution, at 30º C, for 2 h; 2) longitudinal bisection, with subsequent immersion of one seed half into a 1.0% tetrazolium solution, at 30º C, for 3 h; and 3) longitudinal bisection, with subsequent immersion of one seed half into a 0.075% tetrazolium solution, at 40º C, for 2 h. It was concluded that the tetrazolium test is more efficient in evaluating wheat seed vigor when performed with immersion of one half of the seed into a 0.075% tetrazolium solution (40º C, for 2 h) or a 0.1% tetrazolium solution (30º C, during 3 h); allowing to sort seeds into four quality classes.

scholarly journals Fungal and seed treatment interference in the viability of coriander seeds

2021 ◽  
Vol 39 (4) ◽  
pp. 376-382
Author(s):  
Gizele Ingrid Gadotti ◽  
Raimunda Nonata O da Silva ◽  
Cândida Renata J de Farias ◽  
Joseano G da Silva ◽  
Henrique L Padão
Keyword(s):  
Seed Treatment ◽  
Germination Test ◽  
Fungicide Treatment ◽  
Physiological Performance ◽  
Negative Effect ◽  
Health Test ◽  
Tetrazolium Test ◽  
Coriander Seed

ABSTRACT The determination of the sanitary quality is important to diagnose if the commercialized lots are free of pathogens and to make a decision about the need for seed treatment. The objective was to evaluate the interference of fungi associated with coriander seed lots in their physiological performance and the effect of seed treatment with the fungicide Metalaxyl-m + Fludioxonil. The study was carried out in two steps. In experiment I, the physiological potential and sanitary characterization of 18 coriander seed lots were evaluated, using the tests, water content, tetrazolium test and health test. In experiment II, we evaluated the physiological performance of coriander seeds with and without fungicide treatment using the first count and germination test. Coriander seed lots showed high physiological potential, however, not all lots expressed their maximum potential in the germination test without treatment, due to the negative effect of fungi associated with seeds, mainly A. dauci and in association with A. alternata. There was an improvement in the physiological performance of coriander seeds treated with Metalaxyl-m + Fludioxonil fungicide.

scholarly journals Imagens digitalizadas na avaliação do teste de tetrazólio em sementes de trigo

2020 ◽  
Vol 16 (6) ◽  
pp. 67-78
Author(s):  
Emanueli Bastos Garcia ◽  
Marizangela Rizzatti Ávila ◽  
Nelson da Silva Fonseca Júnior ◽  
Getulio Takashi Nagashima
Keyword(s):  
Correlation Coefficients ◽  
Test Results ◽  
Simple Correlation ◽  
Precise Analysis ◽  
Physiological Quality ◽  
Digitized Images ◽  
Tetrazolium Test ◽  
Wheat Seeds

The tetrazolium test can be an alternative to obtain fast results of the physiological potential of wheat seeds. In this context, the objective of the present study was to evaluate the efficiency of the tetrazolium test through the evaluation by analysis of digitized images in the determination of the physiological quality of wheat seeds.The experiment was carried out with 22 lots of wheat seeds, submitted to the determination of the physiological potential, including the tetrazolium test evaluated by means of digitized image analysis.For digitization, after longitudinal bisection, and staining in 0.075% tetrazolium solution, the seeds were grouped on tabletop scanner glass, stored and classified into four vigor classes. Data were submitted to analysis of variance, with means grouped by the Scott-Knott test, at 5% of significance; we also performed an analysis of the simple correlation coefficients between tetrazolium test results and other tests.The evaluation of the tetrazolium test through the analysis of digitized images grouped the lots in three levels of vigor.The method is effective in determining the vigor and viability of wheat seeds, because the possibility of expanding the images that allows precise analysis of the embryonic axis structures.

RAPID DETERMINATION OF SEED GERMINATION POTENTIAL TO MEASURE QUALITY LOSS IN STORED WHEAT

1982 ◽  
Vol 62 (4) ◽  
pp. 1045-1048 ◽  
Author(s):  
N. D. G. WHITE ◽  
R. N. SINHA ◽  
W. E. MUIR
Keyword(s):  
Seed Germination ◽  
Moisture Content ◽  
Rapid Method ◽  
Rapid Determination ◽  
Quality Loss ◽  
Wheat Seed ◽  
Stored Wheat ◽  
Germination Potential

A rapid method of determining wheat seed germination after 1 day (G1) of incubation was found. Germination of wheat seed stored at [Formula: see text] moisture content is estimated by adding 12% to G1; at <17% moisture content germination is not consistently predicted by G1.

scholarly journals PHYSIOLOGICAL POTENTIAL AND CONSERVATION OF MURUCI (Byrsonima crassifolia) SEEDS

2017 ◽  
Vol 39 (5) ◽  
Author(s):  
CAMILA RIBEIRO DE SOUZA GRZYBOWSKI ◽  
WALNICE MARIA OLIVEIRA DO NASCIMENTO ◽  
ROSEMEIRE CARVALHO DA SILVA ◽  
ELISA SERRA NEGRA VIEIRA ◽  
MARISTELA PANOBIANCO
Keyword(s):  
Block Design ◽  
Rapid Assessment ◽  
Germplasm Collection ◽  
Germination Test ◽  
Byrsonima Crassifolia ◽  
Randomized Block Design ◽  
Relative Air Humidity ◽  
Different Temperatures ◽  
Mother Plants ◽  
Tetrazolium Test

ABSTRACT The goal of the present study is to assess the physiological potential of Byrsonima crassifoliaseeds and analyze the procedures of the germination and tetrazolium tests, as well as the conservation of such seeds by testing different combinations of packaging and environment. The experiment used pyrenes of the clone Açu, collected from mother plants in the germplasm collection of muruci plants of Embrapa Amazonia Oriental, in Belém, Brazil. Different temperatures were tested in the germination test (25, 30, 35 and 20 - 30 ºC). The best methodology and assessment dates were determined for the test, and the seedlings of the species were characterized. Viability was assessed with the tetrazolium test, with combinations of hydration methods (by immersion and between paper towels), staining periods (3 and 4 h) and concentrations of tetrazolium solution (0.5, 0.7 and 1.0%). Storage of muruci pyrenes was evaluated at three, six and twelve months, in polyethylene and Kraft paper bags, under dry chamber and refrigerator conditions. The study used a randomized block design with four replicates. The data underwent analysis of variance. In conclusion, the physiological potential of seeds of B. crassifoliacan be assessed by the germination test conducted in sand, with alternating temperatures of 20-30 ºC, or with the use of the tetrazolium test (with staining for three hours in the tetrazolium solution at 1.0%) as a rapid assessment of viability. Muruci pyrenes can be kept in polythene or paper Kraft bags for up to 12 months, in a dry chamber at 16 ºC and with relative air humidity of 50 to 60%.

scholarly journals Adjustment of the methodology of the tetrazolium test for estimating viability of Eugenia uniflora L. seeds during storage

2014 ◽  
Vol 36 (3) ◽  
pp. 344-351 ◽  
Author(s):  
Daiana Karoline Kaiser ◽  
Laura Cristiane Nascimento de Freitas ◽  
Rubia Priscila Biron ◽  
Simone Cristina Simonato ◽  
Michele Fernanda Bortolini
Keyword(s):  
Seed Viability ◽  
Germination Test ◽  
Preparation Methods ◽  
Eugenia Uniflora ◽  
Paper Towel ◽  
Plastic Bags ◽  
Longitudinal Cutting ◽  
Direct Immersion ◽  
Tetrazolium Test

The study aimed to adjust the methodology of the tetrazolium test to estimate seed viability of Eugenia uniflora L. (Surinam Cherry). Seeds were collected in September 2012 and divided into four lots: freshly harvested (Lot I); stored in plastic bags in a refrigerator at 10 °C for: 15 days (Lot II); 30 days (Lot III); and 45 days (Lot IV). The freshly harvested seeds were preconditioned with direct immersion in water and wet paper towel, followed or not by longitudinal cutting. The seeds were immersed in a 0.1% tetrazolium solution for 4 hours at 30 °C. Appropriate soaking and preparation methods were applied to the seeds lots using three tetrazolium concentrations: 0.1; 0.5; and 1.0%; and four preconditioning periods (2, 4, 6 and 8 hours) at 30 °C. The viability results obtained by the tetrazolium test were compared with those of the germination test. Direct immersion of seeds in water for 24 hours at 25 °C, followed by a longitudinal cut was efficient for preconditioning the seeds. Seed staining with tetrazolium solution at a concentration of 0.5% for 2 hours at 30 °C can be used to estimate the viability of freshly harvested and stored Surinam Cherry seeds.

scholarly journals Foliar application of silicon and the effect on wheat seed yield and quality

2013 ◽  
Vol 35 (1) ◽  
pp. 86-91 ◽  
Author(s):  
Samantha Rigo Segalin ◽  
Caroline Huth ◽  
Thais D'Avila Rosa ◽  
Diógenes Barella Pahins ◽  
Liliane Marcia Mertz ◽  
...  
Keyword(s):  
Seed Yield ◽  
Foliar Application ◽  
Accelerated Aging ◽  
Dry Weight ◽  
Wheat Cultivars ◽  
Wheat Seed ◽  
Germination Test ◽  
Yield And Quality ◽  
Physiological Quality

Adequate nutrients for plants are important for increasing the yield and quality of the seeds produced. The objective of this study was to evaluate foliar fertilization with silicon in wheat and its effect on seed yield and physiological quality. Treatments consisted of two silicon dosages (three and six liters silicon per hectare) and the control (no silicon) and five wheat cultivars: OR "Quartzo", OR "Ônix", Fundacep "Linhagem", Fundacep "Campo Real" and Fundacep "Horizonte". The experimental design was randomized blocks with four replications. After physiological maturity, harvesting and threshing of the seeds were done manually. Seed samples were evaluated for yield and physiological quality from the germination test, first germination count, seedling dry weight, accelerated aging, electrical conductivity, 1000 seeds and hectoliter weights. The results showed that the foliar application of silicon at the dosages tested did not affect the yield and physiological quality of the seeds produced by the wheat cultivars.

scholarly journals Determination of Main Spectral and Luminescent Characteristics of Winter Wheat Seeds Infected with Pathogenic Microflora

Photonics ◽  
2021 ◽  
Vol 8 (11) ◽  
pp. 494
Author(s):  
Alexey M. Bashilov ◽  
Igor Yu. Efremenkov ◽  
Mikhail V. Belyakov ◽  
Alexander V. Lavrov ◽  
Anatoly A. Gulyaev ◽  
...  
Keyword(s):  
Winter Wheat ◽  
Physical Properties ◽  
Agricultural Products ◽  
High Quality ◽  
Crop Losses ◽  
Software Complex ◽  
Fusarium Infection ◽  
Constant Growth ◽  
Wheat Seeds

In connection with the constant growth of demand for high-quality food products, there is a need to develop effective methods for storing agricultural products, and the registration and predicting infection in the early stages. The studying of the physical properties of infected plants and seeds has fundamental importance for determining crop losses, conducting a survey of diseases, and assessing the effectiveness of their control (assessment of the resistance of crops and varieties, the effect of fungicides, etc.). Presently, photoluminescent methods for diagnosing seeds in the ultraviolet and visible ranges have not been studied. For research, seeds of winter wheat were selected, and were infected with one of the most common and dangerous diseases for plants—fusarium. The research of luminescence was carried out based on a hardware–software complex consisting of a multifunctional spectrofluorometer “Fluorat-02-Panorama”, a computer with software “Panorama Pro” installed, and an external camera for the samples under study. Spectra were obtained with a diagnostic range of winter wheat seeds of 220–400 nm. Based on the results obtained for winter wheat seeds, it is possible to further develop a method for determining the degree of fusarium infection.

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