Cuttings of Euphorbia phosphorea Mart and Euphorbia enterophora Drake at different concentrations of indole-butyric acid and analysis of economic viability

Abstract Defining seedling production protocols for ornamental species through the use of plant regulators requires economic analysis that demonstrates the real financial returns of such activity to horticulturists. The objective was to evaluate the influence of using indole butyric acid regulator (IBA) on rooting of Euphorbia phosphorea (Mart) and Euphorbia enterophora (Drake) cuttings, as well as to analyze the economic viability of seedling production with the use of IBA. The cuttings were 10 cm long, and treated with IBA at concentrations of 0; 1,000; 3,000; and 5,000 mg L-1. The cuttings were place in vermiculite substrate and were irrigated daily inside a greenhouse. The experimental design was completely randomized, with 4 replications, containing 5 cuttings per experimental unit. Evaluations were performed 150 days after establishing the experiments. The Net Present Value (NPV) and the Internal Return Rate (IRR) were analyzed to determine the economic viability analysis of using IBA. For E. phosphorea there was no significant difference between treatments for cuttings survival, number of roots and length of roots. Callus formation was not observed at the base of the cuttings, and the highest survival percentage was observed in T3 (3,000 mg L-1) and T4 (5,000 mg L-1) treatments. Treatment T1 (control) presented a lower number of roots per cutting (12.8) and higher average length of the three largest roots (12.56 cm). The use of the IBA regulator increased the percentage of rooted cuttings and the number of roots per cutting, and the concentration of 3,000 mg L-1 was recommended for E. phosphorea. Regarding aspects of economic viability, using IBA at the commercial nursery level is recommended. For E. enterophora, IBA is not recommended because it is an easily rooted species (95%), therefore, denotes the economic unfeasibility of its use.

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Study of indole butyric acid (IBA) effects on cutting rooting improving some of wild genotypes of damask roses (Rosa damascena Mill.)

Journal of Agricultural Sciences Belgrade ◽

10.2298/jas1503263n ◽

2015 ◽

Vol 60 (3) ◽

pp. 263-275 ◽

Cited By ~ 2

Author(s):

Fardin Nasri ◽

Arsalan Fadakar ◽

Mahmood Saba ◽

Bayzid Yousefi

Keyword(s):

Butyric Acid ◽

Control Treatment ◽

Rosa Damascena ◽

Indole Butyric Acid ◽

Significant Difference ◽

Maximum Root Length ◽

Number Of Leaves ◽

Maximum Root ◽

Medicinal Properties ◽

The Rose

Rosa damascena is very important for essential oil production, medicinal properties and it is also widely cultivated as a garden rose. The Rose species is mainly propagated by stem cutting. In the present study, the effect of different levels of 0, 500 and 1,000 mg l-1 (quick dip method for 20 s) of indole butyric acid (IBA) on the rooting of 12 wild genotypes (including: Kurdistan 1 to Kurdistan 12) of R. damascena was investigated. The results show that the rooting ability of R. damascena differs significantly between the twelve genotypes. The highest rooting (79.56%) and callus production (69.08%), number of roots (8.33), root fresh and dry weights (361.80 and 244.74 mg, respectively) were recorded in Kurdistan 5 genotype with 1,000 mg l-1 IBA. The maximum root length (5.84 cm) was observed in Kurdistan 5 genotype with 500 mg l-1 IBA that showed a significant difference compared to the control treatment (0.96 cm). The highest number of leaves per bud (7.33 at 500 mg l-1 IBA) and number of buds (5.00 at 1,000 mg l-1 IBA) were recorded in Kurdistan 1 genotype. The current study demonstrated that the different genotypes of R. damascena were in a difficult-toroot state, which suggests that cutting treatment with 1,000 mg l-1 IBA overcame the problem of the difficult-to-root state, and it can also enhance the rooting percentage in the studied genotypes.

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Effect of IBA concentration on the production of coratina olive plant

International Journal of Advances in Scientific Research ◽

10.7439/ijasr.v3i11.4411 ◽

2017 ◽

Vol 3 (11) ◽

pp. 125

Author(s):

Ayesha Ameen ◽

Jalil Ahmad ◽

Shahid Raza

Keyword(s):

Butyric Acid ◽

Shoot Formation ◽

Indole Butyric Acid ◽

Survival Percentage ◽

Formation Number ◽

Number Of Branches

The effect of different concentrations of indole butyric acid (IBA) at 4g/L and 0.12/31.2 ml hardwood and semi hardwood cuttings of coratina olive was observed. Cuttings were taken in the month of February from coratina plant and dipped for 10 seconds in the required IBA solutions. The highest survival percentage, shoot formation, number of roots formation was maximum in 4g/L treated hardwood cuttings. The maximum number of branches were observed in hardwood cuttings as compared to softwood cuttings. It was concluded that either type of olive cutting treated with IBA solution 4g/L showed best results to propagate coratina olive.

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Growth Response of Two Pepper (Piper nigrum L.) Stem Cuttings on Application of IBA (Indole Butyric Acid) and NAA (Naphthalene Acetic Acid)

Indonesian Journal of Agricultural Research ◽

10.32734/injar.v1i1.171 ◽

2018 ◽

Vol 1 (1) ◽

pp. 87-95

Author(s):

Rizky Wulandari ◽

Yaya Hasanah ◽

Meiriani Meiriani

Keyword(s):

Acetic Acid ◽

Butyric Acid ◽

Growth Response ◽

Block Design ◽

Primary Branch ◽

Piper Nigrum ◽

Naphthalene Acetic Acid ◽

Secondary Branch ◽

Indole Butyric Acid ◽

Significant Difference

Using fruit branch for pepper shrub propagation is one of alternatives for an efficient pepper multiplication which usually uses underlayer cuttings. This research is aimed at finding the growth response of two pepper (Piper nigrum L.) cuttings to the administration of IBA (Indole Butyric Acid) and NAA (Naphthalene Acetic Acid). This research was conducted at the greenhouse of the Faculty of Agriculture, University of Sumatera Utara, Medan (± 32 m above sea level), from April to August 2017 using a factorial randomized block design with 2 factors, pepper branch cuttings (primary branch cuttings and secondary branch cuttings) and the administration of IBA and NAA (0+0 ppm, 2500+0 ppm, 0+2500 ppm, 1500+1000 ppm, and 1000+1500 ppm). The results show that the emerging shoot rate in the secondary branch cuttings was significantly faster than in the primary branch cuttings, but the volume of root in the primary branch cuttings is significantly larger than in the secondary branch cuttings. There was no significant difference in the administration of IBA and NAA on all observed variables. The highest interaction of shoot length was found in the primary branch cuttings with the administration of IBA 1500 ppm + NAA 1000 ppm and the highest percentage of root and root volume was found in the primary branch cuttings with the administration of IBA 2500 ppm + NAA 0 ppm.

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Guabiju tree propagation by mini-cutting: ontogeny of the matrix, cutting length and indole-butyric acid

Scientia Agraria Paranaensis ◽

10.18188/sap.v20i1.25600 ◽

2021 ◽

pp. 93-96

Author(s):

Adriana Dallago ◽

Américo Wagner Júnior ◽

Cristiano Hossel ◽

Jessica Scarlet Alves de Oliveira Hossel ◽

Alexandre Hack Porto

Keyword(s):

Experimental Design ◽

Aerial Part ◽

Experimental Unit ◽

Juvenile Period ◽

Indole Butyric Acid ◽

Survival Percentage ◽

The Matrix ◽

Cutting Length ◽

Number Of Leaves ◽

Propagation Technique

Guabiju tree is usually propagated by seeds, although this method presents disadvantages, such as a long juvenile period and great genetic variability, resulting in uneven plants which makes management and the establishment of commercial orchards difficult, in addition to the delay in the production entry. The objective of this work was to test the propagation technique by mini-cutting in guabiju tree. The experimental design used was completely randomized, with a 2 x 2 x 3 factorial (ontogeny of the matrix plant x cut length x IBA concentration), with four replications and 20 mini-cuttings per experimental unit. A hundred and twenty days after the implantation of the experiment, the rooting percentage and callogenesis of the mini-cuttings, the secondary root total length, the aerial part and number of leaves were evaluated. The rooted mini-cuttings were transplanted into larger containers with substrate, and after 60 days of transplantation, their survival percentage was analyzed. The highest rooting percentage was obtained with 0 mg L-1 of IBA with an 57.69% average. Mini-cuttings had a high percentage of survival, with 87.5% being the lowest, presenting a satisfactory result. For the guabiju tree propagation by mini-cutting, it is recommended not to use IBA and adopt a length of four centimeters, using a juvenile matrix plant.

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Effect of Hormones (IBA & IAA) on the Propagation of Himalayan Yew in Pakistan: A Conservation Approach

10.21203/rs.3.rs-27841/v1 ◽

2020 ◽

Author(s):

Javaid Iqbal ◽

Bushra Khan ◽

Sardar Khan ◽

Nasreen Ghaffar ◽

Ishaq Ahmad Mian ◽

...

Keyword(s):

Acetic Acid ◽

Medicinal Plants ◽

Butyric Acid ◽

Indole Acetic Acid ◽

Average Length ◽

Stem Cuttings ◽

Indole Butyric Acid ◽

Number Of Leaves ◽

Himalayan Yew ◽

Taxus Wallichiana

Abstract Background Himalayan yew (Taxus wallichiana) is one of the endangered medicinal plants species having great importance due to the presence of anticancer drug Taxol. This metabolite is mainly used for the treatment of ovarian, breast, AIDS-related cancers, and other indications. The study being reported here was conducted for the propagation of Himalayan yew by using two different trials of Indole Butyric acid (IBA) and Indole acetic acid (IAA) hormones treatments (2000–7000 ppm) through stem cuttings (140 each). In the same way, 3840 cuttings were treated with IBA and IAA from November 2016 to November 2017 at Lalku valley, Swat, Khyber Pakhtunkhwa (KP), Pakistan. Results The influence of IBA treatment (7000 ppm) showed a survival of 85.22% (average number of roots = 10.4, average length of roots = 15.5 cm, average number of leaves = 92.4 and average number of sprouts = 3.3) while that of IAA treatment (7000 ppm) the survival of 81.11% (average number of roots = 9.1, average length of roots = 14.6 cm, average number of leaves = 84.0 and average number of sprout = 3.0) were more significant followed by 2000–6000 ppm (IBA and IAA). Lowest survival 40–45% (average number of roots = 4.2, average length of roots = 8.0 cm, average number of leaves = 32.2, average number of sprouts = 1.7) was noticed for controlled cuttings. Conclusion The present study enhanced the potential of conservation and propagation of T. wallichiana. Hence our study suggests and recommends the application of IBA (7000 ppm) as a better hormone for the conservation and propagation of Himalayan yew.

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Maximum efficiency concentration of indole butyric acid in promoting the rooting of Japanese Flowering Cherry

Ciência Rural ◽

10.1590/0103-8478cr20150894 ◽

2017 ◽

Vol 47 (1) ◽

Author(s):

Rosimeri de Oliveira Fragoso ◽

Carlos André Stuepp ◽

Helena Cristina Rickli ◽

Katia Christina Zuffellato-Ribas ◽

Henrique Soares Koehler

Keyword(s):

Butyric Acid ◽

Root Formation ◽

Gradual Increase ◽

Callus Formation ◽

Maximum Efficiency ◽

Stem Cuttings ◽

Ambient Conditions ◽

Indole Butyric Acid ◽

Flowering Cherry ◽

Low Efficiency

ABSTRACT: Prunus serrulata is a species widely used in the ornamentation and its economic importance is mainly due to the time of its flowering. The objective was to assess the feasibility of cutting technique for propagating P. serrulata , using stem cuttings from current-year shoots, and to estimate the maximum efficiency concentration of auxin indole butyric acid (IBA), in the promotion of rooting. Collection of plant material took place in late spring. Cuttings were made with about 8±1cm length and two leaves reduced to 50% of its original surface. Twenty one IBA concentrations have been assessed (0, 250, 500, 750, 1000, 1250, 1500, 1750, 2000, 2250, 2500, 2750, 3000, 3250, 3500, 3750, 4000, 4250, 4500, 4750 e 5000mg L-1), applied in base of cuttings, which were placed in acclimatized greenhouse. After 60 days, there was a gradual increase in the rooting percentage compared to the increment of IBA to the approximate concentration of 2700mg L-1, with results higher than 80%. In addition, low efficiency concentrations of growth regulators have resulted in fewer roots per cutting, which could compromise the survivability of the seedlings under ambient conditions. Conversely, the decrease in the percentage of callus may be an indication that root formation in P. serrulata occurs directly, without requiring callus formation and its further de-differentiation and re-differentiation in roots.

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Investigation of Changes in Phytohormone Levels Depending on Effects of Exogenous Indole-Butyric-Acid and Callus Formation in the Stem Cuttings of Some Apple Kinds (Malus sylvestris Miller)

Asian Journal of Plant Sciences ◽

10.3923/ajps.2007.1103.1107 ◽

2007 ◽

Vol 6 (7) ◽

pp. 1103-1107

Author(s):

Aysel Sivaci . ◽

Ibrahim Yalcin .

Keyword(s):

Butyric Acid ◽

Callus Formation ◽

Stem Cuttings ◽

Indole Butyric Acid ◽

Malus Sylvestris

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Effect of different concentrations of indole butyric acid, putrescine and hydrogen peroxide on stem cuttings of the rootstock GF677(Prunus amygdalus × Prunus persica) according to the cutting season

Revista Facultad Nacional de Agronomía Medellín ◽

10.15446/rfnam.v74n2.92414 ◽

2021 ◽

Vol 74 (2) ◽

Author(s):

Sina Kordzadeh ◽

Hassan Sarikhani

Keyword(s):

Hydrogen Peroxide ◽

Butyric Acid ◽

Interspecific Hybrid ◽

Prunus Persica ◽

Callus Formation ◽

Stem Cuttings ◽

Prunus Amygdalus ◽

Fresh Weight ◽

Indole Butyric Acid ◽

Rooting Of Cuttings

The rootstock GF677 is an interspecific hybrid with an important economic and horticultural value. In this research, the effect of indole butyric acid (IBA) in combination with putrescine (Put) and hydrogen peroxide (H2O2) on rooting of GF677 semi-hardwood stem cuttings in three cutting seasons (July, March and October) was investigated. Treatments as IBA (0, 1000, 2000 and 3000 mg L-1), Put (0, 800, 1600 and 3200 mg L-1) and H2O2 (1.5, 3 and 6% w/v) were included. The results showed that in July cuttings, the highest levels of callogenesis were observed in IBA treated cuttings in both concentrations of 1000 and 2000 mg L-1. The rooting was very low in July cuttings, while the highest percentage of rooting (14%) was observed in the combination of 2000 mg L-1 IBA+ 3% H2O2. In March, the cuttings treated by 1000 mg L-1 IBA+800 mg L-1 Put and 1000 mg L-1 IBA+1600 mg L-1 Put revealed the highest percentages of callus formation 83.31 and 83.33%, respectively. In these cuttings, the highest percentage of rooting (63.88%) was gained at 2000 mg L-1 IBA+3200 mg L-1 Put. The application of 1000 mg L-1 IBA+800 mg L-1 Put increased root fresh weight. In cuttings prepared in October, only 800 mg L-1 Put caused callus formation in more than 55% of the cuttings. The rooting of cuttings at this time was as low as the July cuttings, whereas the highest rooting percentage was observed in cuttings treated with IBA at a concentration of 1000 mg L-1. Overall, the experiment showed that the season of the cutting and the treatments with IBA+Put or H2O2 could improve rooting properties of the rootstock GF677.

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Respon Setek Cabang Bambu Betung (Dendrocalamus Asper) Akibat Pemberian Asam Indol Butirat (AIB)

Jurnal Sylva Lestari ◽

10.23960/jsl2461-68 ◽

2016 ◽

Vol 4 (2) ◽

pp. 61

Author(s):

Erviana Sari ◽

Indriyanto . ◽

Afif Bintoro

Keyword(s):

Butyric Acid ◽

Experimental Unit ◽

Development Research ◽

Indole Butyric Acid ◽

Randomized Design ◽

Shoot Number ◽

Bamboo Plantation ◽

Dendrocalamus Asper ◽

Completely Randomized Design ◽

Branch Cutting

Betung bamboo (Dendrocalamus asper) as multipurpose plant could be developed with vegetative and generative propagation. Branch cuttings was the easiest way in the betung bamboo vegetative propagation. One of plant growth regulators that could be used to spur betung bamboo branch cutting was indole butyric acid (IBA). This study was conducted to evaluate the betung bamboo branch cutting response from various concentration of IBA and to know the best concentration of IBA to spur the growth. The experiment was conducted in Palembang’s Bamboo Plantation Collection and Development Research Institute of Forest areal, Tanjung Agung, District Tanjungan, South Lampung Regency, from March to June 2012. This research used Completely Randomized Design (CRD) with five treatments: IBA 0 ppm (control), 100 ppm, 200 ppm, 300 ppm, and 400 ppm of concentration. Each of experimental unit consisted of five cuttings with five replications. Percentage of life, length of shoot, number of shoot, number of leave, shoot’s diameter, and root’s length were the observed variables. The result showed that betung bamboo branch cuttings were responsive to IBA, and IBA 200 ppm concentration gave better results to the betung bamboo branch cuttings than IBA 0 ppm (control), 100 ppm, 300 ppm, and 400 ppm concentration. Based on this study, it was recomended to use 200 ppm of IBA for betung bamboo branch cutting. Keywords : betung bamboo, branch cutting, indole butyric acid

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THE EFFECT OF PICLORAM AND LIGHT ON SOMATIC EMBRYOGENESIS REGENERATION OF PINEAPPLE

Indonesian Journal of Agricultural Science ◽

10.21082/ijas.v13n2.2012.p43-53 ◽

2012 ◽

Vol 13 (2) ◽

pp. 43 ◽

Cited By ~ 1

Author(s):

Ika Roostika ◽

Nurul Khumaida ◽

Ika Mariska ◽

Gustaaf Adolf Wattimena

Keyword(s):

Somatic Embryogenesis ◽

Butyric Acid ◽

Somatic Embryos ◽

Callus Formation ◽

Light Condition ◽

Cell Polarization ◽

Mass Propagation ◽

Benzyl Adenine ◽

Indole Butyric Acid ◽

Embryogenic Tissues

Smooth Cayenne is the largest pineapple type cultivated in Indonesia, but its vegetative planting materials for mass propagation are limited. Somatic embryogenesis is a potential method to be applied. The aim of this study was to investigate the somatic embryogenesis regeneration under the effect of picloram and light. Callus formation was induced by picloram (21, 41 and 62 μM) added with 9 μM thidiazuron. The calli were transferred onto MS or Bac medium enriched with N-organic compounds with or without addition of 21 μM picloram under dark or light condition. The compact calli were subcultured onto MS medium supplemented with 4.65 μM kinetin, while the friable calli were transferred onto BIG medium (modified MS + 1.1 μM benzyl adenine + 0.9 μM indole butyric acid + 0.09 μM giberelic acid) or B medium (MS + 0.018 mM benzyl adenine). The results showed that the events of somatic embryogenesis were started from cell polarization, asymmetrical division, proembryo formation as embryogenic tissues and friable embryogenic tissues, and embryo development. The best treatment for callus induction was 21 μM picloram. The addition of 21 μM picloram on N-organic enriched medium and the use of light condition proliferated embryogenic calli. The N-organic enriched Bac medium and light condition yielded the highest number of mature somatic embryos (17 embryos per explant in 2 months). The B medium was better than BIG medium to develop somatic embryos from friable embryogenic tissues. The somatic embryogenesis method presented is potential for pineapple mass propagation and artificial seed production.Abstrak Bahasa IndonesiaSmooth Cayenne merupakan kultivar nenas yang banyak dibudidayakan di Indonesia, namun ketersediaan benih untuk perbanyakan massal masih terbatas. Embriogenesis somatikadalah metode yang potensial untuk produksi bibit secara massal. Tujuan penelitian adalah untuk mempelajari pengaruh pikloram dan pencahayaan terhadap regenerasi embriogenesis somatik nenas. Kalus diinduksi menggunakan pikloram (21, 41, dan 62 μM) dan penambahan thidiazuron 9 μM. Selanjutnya, kalus dipindahkan ke media MS atau Bac yang diperkaya dengan senyawa N-organik dengan atau tanpa penambahan pikloram 21 μM dalam kondisi gelap atau dengan pencahayaan. Kalus kompak disubkultur pada media MS yang mengandung kinetin 4,65 μM, sedangkan kalus remah dipindahkan ke media BIG (MS modifikasi + bensil adenin 1.1 μM + indole butyric acid 0,9 μM + giberelic acid 0,09 μM) atau media B (MS + bensil adenin 0,018 μM). Hasil penelitian menunjukkan bahwa tahapan embriogenesis somatik diawali dengan polarisasi sel, pembelahan asimetris, pembentukan proembrio sebagai jaringan embriogenik dan jaringan embriogenik remah, serta perkembangan embrio. Perlakuan terbaik untuk induksi kalus adalah pikloram 21 μM. Penambahan pikloram 21 μM pada media yang diperkaya dengan senyawa N-organik mampu meningkatkan jumlah kalus embriogenik. Media Bac yang diperkaya senyawa N-organik dan kondisi pencahayaan menghasilkan jumlah embrio somatik dewasa terbanyak (17 embrio per eksplan dalam 2 bulan). Media B lebih baik daripada media BIG untuk regenerasi embrio somatik dari jaringan embriogenik remah. Metode embriogenesis somatik yang dihasilkan dari penelitian ini berpotensi diterapkan untuk perbanyakan massal dan produksi benih nenas.

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