scholarly journals The effect of temperature on mobility of Angiostrongylus costaricensis third stage larvae

1999 ◽  
Vol 41 (4) ◽  
pp. 225-228 ◽  
Author(s):  
Lúcia M.Z. RICHINITTI ◽  
Nivaldo A. FONSECA ◽  
Carlos GRAEFF-TEIXEIRA
Keyword(s):  
Mathematical Model ◽  
Room Temperature ◽  
Effect Of Temperature ◽  
Infective Larvae ◽  
Third Stage ◽  
Albino Mice ◽  
Time Required

Third stage larvae (L3) from Angiostrongylus costaricensis were incubated in water at room temperature and at 5 <FONT FACE="Symbol">°</font> C and their mobility was assessed daily for 17 days. Viability was associated with the mobility and position of the L3, and it was confirmed by inoculation per os in albino mice. The number of actively moving L3 sharply decreased within 3 to 4 days, but there were some infective L3 at end of observation. A mathematical model estimated 80 days as the time required to reduce the probability of infective larvae to zero. This data does not support the proposition of refrigerating vegetables and raw food as an isolated procedure for prophylaxis of human abdominal angiostrongylosis infection.

Effect of Temperature on ADP-Induced Platelet Aggregation

1973 ◽  
Vol 29 (01) ◽  
pp. 183-189
Author(s):  
C. A Praga ◽  
E. M Pogliani
Keyword(s):  
Platelet Aggregation ◽  
Incubation Period ◽  
Room Temperature ◽  
Important Variable ◽  
Practical Significance ◽  
Effect Of Temperature ◽  
Induce Platelet Aggregation ◽  
Cuvette Holder ◽  
Exact Temperature

SummaryTemperature represents a very important variable in ADP-induced platelet aggregation.When low doses of ADP ( < 1 (μM) are used to induce platelet aggregation, the length of the incubation period of PRP in the cuvette holder of the aggregometer, thermostatted at 37° C, is very critical. Samples of the same PRP previously kept at room temperature, were incubated for increasing periods of time in the cuvette of the aggregometer before adding ADP, and a significant decrease of aggregation, proportional to the length of incubation, was observed. Stirring of the PRP during the incubation period made these changes more evident.To measure the exact temperature of the PRP during incubation in the aggre- gometer, a thermocouple device was used. While the temperature of the cuvette holder was stable at 37° C, the PRP temperature itself increased exponentially, taking about ten minutes from the beginning of the incubation to reach the value of 37° C. The above results have a practical significance in the reproducibility of the platelet aggregation test in vitro and acquire particular value when the effect of inhibitors of ADP induced platelet aggregation is studied.Experiments carried out with three anti-aggregating agents (acetyl salicyclic acid, dipyridamole and metergoline) have shown that the incubation conditions which influence both the effect of the drugs on platelets and the ADP breakdown in plasma must be strictly controlled.

scholarly journals Green Biosynthesis of Silver Nanoparticles Using Leaf Extract of Carissa carandas L. and Their Antioxidant and Antimicrobial Activity against Human Pathogenic Bacteria

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 299
Author(s):  
Reetika Singh ◽  
Christophe Hano ◽  
Gopal Nath ◽  
Bechan Sharma
Keyword(s):  
Silver Nanoparticles ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Room Temperature ◽  
Xrd Analysis ◽  
Antibacterial Activities ◽  
Effect Of Temperature ◽  
Human Pathogenic Bacteria ◽  
Carissa Carandas ◽  
Antioxidant And Antimicrobial Activity

Carissa carandas L. is traditionally used as antibacterial medicine and accumulates many antioxidant phytochemicals. Here, we expand this traditional usage with the green biosynthesis of silver nanoparticles (AgNPs) achieved using a Carissa carandas L. leaf extract as a reducing and capping agent. The green synthesis of AgNPs reaction was carried out using 1mM silver nitrate and leaf extract. The effect of temperature on the synthesis of AgNPs was examined using room temperature (25 °C) and 60 °C. The silver nanoparticles were formed in one hour by stirring at room temperature. In this case, a yellowish brown colour was developed. The successful formation of silver nanoparticles was confirmed by UV–Vis, Fourier transform infrared (FT-IR) and X-ray diffraction (XRD) analysis. The characteristic peaks of the UV-vis spectrum and XRD confirmed the synthesis of AgNPs. The biosynthesised AgNPs showed potential antioxidant activity through DPPH assay. These AgNPs also exhibited potential antibacterial activity against human pathogenic bacteria. The results were compared with the antioxidant and antibacterial activities of the plant extract, and clearly suggest that the green biosynthesized AgNPs can constitute an effective antioxidant and antibacterial agent.

Studies on the life-history of Bunostomum phlebotomum (Railliet, 1900), a hookworm parasite of cattle

Parasitology ◽  
1946 ◽  
Vol 37 (3-4) ◽  
pp. 192-201 ◽  
Author(s):  
J. F. A. Sprent
Keyword(s):  
Infective Larva ◽  
Stage Larva ◽  
Fourth Stage ◽  
Larval Stages ◽  
The Third ◽  
Third Stage ◽  
History Of ◽  
Time Required ◽  
Cutaneous Blood ◽  
Fourth Stage Larva

A description is given of the processes of copulation, formation of the egg and spermatozoon, cleavage, embryogeny and hatching in B. phlebotomum. These processes were found to be essentially similar to those in other strongyle nematodes.The anatomy of the first three larval stages is described and the observations of Conradi & Barnette (1908) and Schwartz (1924) were largely confirmed.Penetration of the skin of calves by the infective larva was observed histologically. The larvae were found to have reached the dermis within 30 min. and to have penetrated the cutaneous blood vessels within 60 min. of application to the skin. The larvae were found in the lung where the third ecdysis was in progress 10 days after penetration of the skin. A description is given of the growth of the third-stage larva in the lung, the changes which take place during the third ecdysis, and the anatomy of the fourth-stage larva.The fourth-stage larvae exsheath in the lungs and travel to the intestine. After a period of growth in which sexual differentiation takes place, the fourth ecdysis occurs and the adult parasite emerges. The time required for the attainment of maturity was found to be somewhere between 30 and 56 days after penetration of the skin.This paper was written at the Ministry of Agriculture and Fisheries Veterinary Laboratories, Wey-bridge, and the writer would like to express his gratitude to the Director, Prof. T. Dalling, also to Dr W. R. Wooldridge, chairman of the Council of the Veterinary Educational Trust for their help and encouragement. The writer's thanks are also due to Dr H. A. Baylis, Prof. R. T. Leiper and Dr E. L. Taylor for their advice and help on technical points, and to Mr R. A. O. Shonekan, African laboratory assistant, for his able co-operation.

STUDIES ON STRONGYLOIDES OF PRIMATES: IV. EFFECT OF TEMPERATURE ON THE MORPHOLOGY OF THE FREE-LIVING STAGES OF STRONGYLOIDES FULLEBORNI

1958 ◽  
Vol 36 (4) ◽  
pp. 623-628 ◽  
Author(s):  
Premvati
Keyword(s):  
Morphological Changes ◽  
Effect Of Temperature ◽  
Optimum Temperature ◽  
Free Living ◽  
Infective Larvae ◽  
Complete Development

The optimum temperature for the complete development of the free-living and the infective larvae of Strongyloides fülleborni is 25 °C. Morphological changes are seen at higher or lower temperatures.

A modified bioassay for heat-stable Escherichia coli enterotoxin

1977 ◽  
Vol 23 (3) ◽  
pp. 331-336 ◽  
Author(s):  
S. Stavric ◽  
D. Jeffrey
Keyword(s):  
Escherichia Coli ◽  
Body Weight ◽  
Incubation Time ◽  
Evans Blue ◽  
Room Temperature ◽  
Blue Dye ◽  
Evans Blue Dye ◽  
Heat Stable ◽  
Stomach Distention ◽  
Time Required

Infant mice were injected orally with preparations containing Escherichia coli heat-stable enterotoxin (ST) and Evans blue dye, and incubated at 22 °C. With enterotoxin-positive samples, the stomach was distended and contained essentially all of the dye. With enterotoxin-negative samples, the stomach remained normal in size and the dye passed freely into the intestines. The time required to obtain the maximum ratio of gut weight to body weight varied from 30 to 90 min and was dependent upon the concentration of enterotoxin. Heat-labile enterotoxin (LT) had no effect during this period.Based on these findings, the mouse incubation time was reduced from 4 h to 90 min, and the heating of test samples was retained only for confirmation of ST. The location of the dye and stomach distention served as an indicator of positive responses to ST. Incubation of the mice at room temperature (22 °C) was found satisfactory.

Analytical Study of Hydro-Pneumatic Processes for Gorlov's Power System

1994 ◽  
Vol 208 (1) ◽  
pp. 67-70
Author(s):  
A I Ryazanov
Keyword(s):  
Experimental Data ◽  
Mathematical Model ◽  
Power System ◽  
Analytical Study ◽  
Model Tests ◽  
Air Pressure ◽  
Pneumatic Power ◽  
Time Required ◽  
The Mathematical Model

This paper describes the aerohydrodvnamics of processes in chambers of Gorlov's hydro-pneumatic power system. The mathematical model is developed to determine the main parameters of the processes: water and air velocities, air pressure in the chamber, the periods of time required to fill and empty the chambers and the output of energy during the cycle. The results obtained are in agreement with experimental data and model tests.

Improved Methods of Obtaining Clean Larvae of Haemonchus contortus

1963 ◽  
Vol 37 (4) ◽  
pp. 251-254 ◽  
Author(s):  
M. G. Christie ◽  
J. E. Patterson
Keyword(s):  
Free Surface ◽  
Surface Area ◽  
Haemonchus Contortus ◽  
Water Repellent ◽  
Optimum Conditions ◽  
Infective Larvae ◽  
Mucous Coat ◽  
Third Stage ◽  
Free Surface Area ◽  
Improved Methods

A pellet of sheep faeces containing eggs of Haemonchus contortus forms an adequate environment for the development of those eggs to third stage infective larvae, provided that it is kept moist. Observation shows a concentration of developing larvae on the external mucous coat of the pellet. These data suggest that optimum conditions for development would occur when pellets are separated one from another and standing on a water repellent surface, thus presenting the maximum free surface area and minimum opportunity for aggregation and hence of over crowding. The best method of recovering the infective larvae would be one that obtained larvae free from contamination without requiring them to expend energy in separating themselves from the contamination.

Studies on resistance of sheep to infestation with Haemonchus contortus and Trichostrongylus spp. and on the immunological reactions of sheep exposed to infestation. I. The preparation of antigens for the complement fixation test and the reactivity of the biochemical fractions of H. contortus

1950 ◽  
Vol 1 (3) ◽  
pp. 285 ◽  
Author(s):  
DF Stewart
Keyword(s):  
Haemonchus Contortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Mature Adult ◽  
Infective Larvae ◽  
Lipid Fractions ◽  
Significant Difference ◽  
Third Stage ◽  
Free Material

All antigen was developed to detect circulating antibodies by means of the complement fixation test in sheep infested with Haemonchus contortus and Trichostrongylus spp. Extraction of worm material at 100°C. for 10 minutes was found to be the most satisfactory method for the preparation of antigens. Potent antigens were prepared from young adult H. contortus, from third-stage infective larvae, and from the eggs. Old mature adult H. contortus yielded antigens of low potency. No significant difference was found between the potency of antigens prepared from male and female adult H. contortus collected from the same sheep. Both adult Trichostrongylus spp. and third-stage infective larvae consistently yielded antigens of high potency. No significant difference was found in the results obtained with larval or adult H. contortus antigens, adjusted to the same potency and tested with natural H. contortus antisera. H. contortus and Trichostrongylus spp. absorbed the antiserum to each other. It was shown that a lipid was an essential constituent of the boiled antigen in the complement fixation reaction with natural antisera. Lipid-free antigens from H. contortus failed to react with natural antisera. The lipid was not antigenic when injected into rabbits. The carbohydrate fraction of H. contortus did not fix complement in the presence of natural antisera and was not antigenic when injected into rabbits. The lipid fraction of a variety of nematode parasites reacted with natural antisera to H. contortus infestation. Similar lipid fractions of two species of trematodes did not react with natural antisera to H. contortus infestation. Normal saline suspensions of the lipid-free material from a variety of helminths showed a greater degree of specificity when tested with artificially prepared antisera than did the lipid fractions.

scholarly journals On using the rotor acceleration mode to increase the accuracy of a two-stage gyrocompass

2020 ◽  
pp. 5-13
Author(s):  
Volodymyr Fedorov ◽  
Vladislav Kikot ◽  
Nataliya Shtefan
Keyword(s):  
Mathematical Model ◽  
Initial Position ◽  
Effect Of Temperature ◽  
Two Stage ◽  
Best Estimate ◽  
Torsion Suspension ◽  
Measured Angle ◽  
State Position ◽  
Acceleration Mode ◽  
The Mathematical Model

The article considers a two-stage gyrocompass with a rigid torsion suspension of the moving part. The principle of its action is based on balancing the elastic moment of the torsion bars with the gyroscopic moment. When this condition is met, the azimuth of the steady-state position of the rotor axis is calculated from the known kinetic moment , latitude and angular rigidity of the torsion bars, and the measured angle of rotation of the moving part of the gyroscope relative to its initial position. The “aging of the material” of the torsion bars, the effect of temperature on them, etc., leads to an uncontrolled change in the angular stiffness of the torsion bars, which, in turn, leads to an error in determining the position of the meridian. A method is proposed for determining the position of the meridian under conditions when the angular stiffness of the torsion is unknown. The method involves observing the motion of the gyroscope in a mode where the kinetic momentum changes linearly (the rotor accelerates). This movement is associated with the movement of the mathematical model of a two-stage gyrocompass in the same mode in the form of a differential equation of motion or in the form of its solution. As a result of minimizing the discrepancy between the real movement of the gyrocompass and the movement of its mathematical model, the “best estimate” of the parameter characterizing the position of the meridian and the “best estimate” of the angular stiffness of the torsion bars in this dimension are found. The results of modeling the corresponding information processing algorithms are considered. The advantages of the proposed method compared with traditional methods are indicated .

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