scholarly journals The epidemiologic profile and prevalence of cardiopathy in Trypanosoma cruzi infected blood donor candidates, Londrina, Paraná, Brazil

2005 ◽  
Vol 47 (6) ◽  
pp. 321-326 ◽  
Author(s):  
Divina Seila de Oliveira-Marques ◽  
Ana Maria Bonametti ◽  
Tiemi Matsuo ◽  
Francisco Gregori Junior

To describe the epidemiologic profile and prevalence of cardiopathy in 163 Trypanosoma cruzi serum positive blood donor candidates, a descriptive study was carried out between August, 1996 and November, 1997 at the Londrina State University Chagas Disease Outpatient Clinic. The profile found was: young, average age 42.95 ± 8.62 years; male (65%); Caucasian (84%); low level of schooling; low family income; agricultural worker (26%); born in the state of Paraná (67%); from rural areas (85%); migrated to the city (85%); and the vector as the main mechanism of transmission. During the clinical characterization a chronic cardiac form was found in 38% of the patients and classified as cardiac suggestive form in 21% and little suggestive of Chagas disease in 17%. No significant difference was found among age group distribution, sex and the presence of cardiac symptoms in patients with or without cardiopathy. This study emphasizes the importance of expanding medical services to areas with a greater prevalence of infected individuals, in a hierarchical manner and aiming at decentralization.

1989 ◽  
Vol 22 (3) ◽  
pp. 119-124 ◽  
Author(s):  
Roberto Chuit ◽  
Elisabet Subias ◽  
Analia C. Pérez ◽  
Irene Paulone ◽  
Cristina Wisnivesky-Colli ◽  
...  

Thirteen communities from 7 Argentinian provinces were selected for the evaluation of serology as an indicator of transmission of Chagas disease. Of the communities appraised, 6 did not have a history of previous treatment with insecticides and 7 had received sporadic or continuous insecticide treatment. The inhabitants of 20% of the houses of each locality were studied by serology. The samples were obtained byfinger pricking and 50 fil of blood were mixed with 150μl of 50% glycerine solution in tissue culture media to be assayed by Indirect Hemagglutination and Indirect Immunofluorescence tests. In untreated areas, the prevalence of infection in infants 0-4 years old was 17.5%, reaching to over 22% for the 5-9 year old group, and to 33.3% in 10-14 year old individuals. The prevalence in treated and surveyed areas was 2.6% in 0-4 year old children, 5.4% in 5-9 year old and 6,2% in 10-14 year old youngsters. The differences between both areas were statistically significant (p < 0.005). This study favors serology as a valid indicator for the evaluation of transmission of Chagas disease in rural areas.


2019 ◽  
Author(s):  
Alexander S.F. Berry ◽  
Renzo Salazar-Sánchez ◽  
Ricardo Castillo-Neyra ◽  
Katty Borrini-Mayorí ◽  
Claudia Arevalo-Nieto ◽  
...  

AbstractAnthropogenic environmental alterations such as urbanization can threaten native populations as well as create novel environments that allow human pests and pathogens to thrive. As the number and size of urban environments increase globally, it is more important than ever to understand the dispersal dynamics of hosts, vectors and pathogens of zoonotic disease systems. For example, a protozoan parasite and the causative agent of Chagas disease in humans, Trypanosoma cruzi, recently colonized and spread through the city of Arequipa, Peru. We used population genomic and phylogenomic tools to analyze whole genomes of 123 T. cruzi isolates collected throughout Arequipa to determine patterns of T. cruzi dispersal. The data show significant population genetic structure within city blocks-parasites in the same block tend to be very closely related - but no population structure among blocks within districts - parasites in neighboring blocks are no more closely related to one another than to parasites in distant districts. These data suggest that T. cruzi dispersal within a block occurs regularly and that occasional long-range dispersal events allow the establishment of new T. cruzi populations in distant blocks. Movement of domestic animals may be the primary mechanism of inter-block and inter-district T. cruzi dispersal.Author SummaryUrbanization creates environments that are ideal for some human pests and pathogens. As the number and size of urban environments increases globally, it is becoming vital to understand how human disease-causing pathogens, their vectors, and their non-human hosts disperse through urban landscapes. Here we study a population of Trypanosoma cruzi – the protozoan parasite and causative agent of Chagas disease in humans – that recently colonized the city of Arequipa, Peru. We use population genomic and phylogenomic tools to understand how this parasite population dispersed through the city to achieve its current distribution and abundance. We show that T. cruzi collected from the same city block tend to be very closely related, while those from neighboring blocks are often as distantly related as those from blocks in distant districts. The data suggest that vectors facilitate frequent within-block dispersal of the parasite, while domestic animal movement may facilitate the relatively infrequent inter-block and interdistrict dispersal.


Author(s):  
Carmen Marín-Tello ◽  
◽  
César Sánchez-Marín ◽  
Luis Arteaga-Temoche ◽  
◽  
...  

Chagas disease, a parasitic infection caused by the protist Trypanosoma cruzi, affect the poorest populations, living in remote, rural areas and urban slums. Although this drug is effective against Chagas disease present a number of serious side effects. In residents of high Andean areas with megacolon it can lead to cardiomyopathies. The aim of this study was to investigate whether dietary supplementation with L-glutamine may alleviate some of these symptoms because of its previously observed anti-inflammatory properties. We studied two groups of T. cruzi-infected mice receiving treatment with nifurtimox. One group was fed the standard diet, while the other group’s diet was supplemented with Glutamine. We found that Glutamine supplementation increases body weight (p<0.001), decreases heart mass to body mass ratio (p<0.001), and decreases the number of amastigotes present in cardiac tissue. Additionally, histopathological analysis showed less heart tissue damage in the group that received Glutaminne in their diet. Therefore, our findings suggest that Glutamine supplementation improves nifurtimox treatment outcomes of T. cruzi infection.


2016 ◽  
Vol 5 (2) ◽  
pp. 9
Author(s):  
Paulo de Tarso Nunes Silva da Costa Júnior ◽  
Alda E. F. Lobato da Cunha

Os triatomíneos, popularmente conhecido como barbeiro, sendo tal o agente transmissor da doença de Chagas no Brasil e nas Américas. Em Porto Velho, município de Rondônia, ainda não se tem estudos de notificações que venha divulgar números de casos da doença de Chagas notificado. O objetivo do estudo foi realizar um levantamento da ocorrência do vetor da doença de Chagas em uma área periurbana da cidade Porto Velho, no bairro Ronaldo Aragão, na linha Progresso. Esta localidade foi selecionada por constante ocorrência do inseto vetor, os quais após coletados por moradores do domicilio foram enviados ao laboratório de entomologia do Centro de Controle de Zoonoses e posteriormente ao LACEN-RO. As coletas entomológicas tiveram duas etapas, em campo e em laboratório, a análise do material coletado foi no laboratório de entomologia médica no LACEN-RO. Os resultados obtidos das 16 coletas, através de busca ativa, com nenhum registro do vetor da Chagas. Mas das buscas passivas, obteve-se um total de 14 exemplares de triatomíneos, das quais houve prevalência da espécie Rhodnius robustus e apenas 01 exemplar de Rhodnius pictipes, e dentre estes, um triatomíneo estava positivo com Trypanosoma cruzi. Sugerimos novas coletas entomológicas, objetivando um maior numero de exemplares de triatomíneos, tanto na área deste estudo, como em outras localidades neste município e outros ecótopos, e ainda realizar o isolamento das amostras de Trypanosoma cruzi para fins da possibilidade da realização de testes moleculares.ABSTRAT The triatomíneos, popularly are known as barber, being such the transmitter agent of the Chagas disease in Brazil and in the Americas. In Porto Velho, city of Rondonia, there are not studies of notifications that come to divulge numbers of cases of chagas notified. The objective of the study was to do a survey of the occurrence for Chagas’ disease vector in the suburbs of the city of Porto Velho, in the Ronaldo Aragao, neighborhood ,in the line Progress. This locality was selected by constant occurrence of the insect vector, which after collected for inhabitants of the home are sent to the laboratory of entomology of the Control Center of Zoonoses and later to the LACEN-RO. The entomologicals collections had two stages, in field and in laboratory, the analysis of the collected material was in the laboratory of medical entomology in the LACEN-RO. The obtained results of the 16 collections, through passive searches, it was obtained a total of 14 types of of triatomíneos , of which had prevalence of the Rhodnius robustus species and only 01 type of Rhodnius pictipes, and among these, a triatomíneo was positive with Trypanosoma cruzi. We suggested new entomologicals collections, objectifying a larger number of triatomíneos types, so much in the area of this study, as in other localities in this city and other environments, and still to do through the isolation of the samples of Trypanosoma cruzi for ends of the possibility of accomplishment of molecular tests.


Parasitology ◽  
2020 ◽  
pp. 1-10
Author(s):  
Marcela S. Rial ◽  
Katia P. Seremeta ◽  
Mónica I. Esteva ◽  
Jacqueline Búa ◽  
Claudio J. Salomon ◽  
...  

Abstract Chagas disease is a serious parasitic infection caused by Trypanosoma cruzi. Unfortunately, the current chemotherapeutic tools are not enough to combat the infection. The aim of this study was to evaluate the trypanocidal activity of benznidazole-loaded microparticles during the acute phase of Chagas infection in an experimental murine model. Microparticles were prepared by spray-drying using copolymers derived from esters of acrylic and methacrylic acids as carriers. Dissolution efficiency of the formulations was up to 3.80-fold greater than that of raw benznidazole. Stability assay showed no significant difference (P > 0.05) in the loading capacity of microparticles for 3 years. Cell cultures showed no visible morphological changes or destabilization of the cell membrane nor haemolysis was observed in defibrinated human blood after microparticles treatment. Mice with acute lethal infection survived 100% after 30 days of treatment with benznidazole microparticles (50 mg kg−1 day−1). Furthermore, no detectable parasite load measured by quantitative polymerase chain reaction and lower levels of T. cruzi-specific antibodies by enzyme-linked immunosorbent assay were found in those mice. A significant decrease in the inflammation of heart tissue after treatment with these microparticles was observed, in comparison with the inflammatory damage observed in both infected mice treated with raw benznidazole and untreated infected mice. Therefore, these polymeric formulations are an attractive approach to treat Chagas disease.


2020 ◽  
Author(s):  
Danya A. Dean ◽  
Gautham ◽  
Jair L. Siqueira-Neto ◽  
James H. McKerrow ◽  
Pieter C. Dorrestein ◽  
...  

AbstractChagas disease (CD) is one of thirteen neglected tropical diseases caused by the parasite Trypanosoma cruzi. CD is a vector-borne disease transmitted by triatomines but CD can also be transmitted through blood transfusions, organ transplants and congenital transmission. While endemic to Latin America, T. cruzi infects 7-8 million people worldwide and can induce severe cardiac symptoms including apical aneurysms, thromboembolisms and arrhythmias during the chronic stage of CD. However, these cardiac clinical manifestations and CD disease pathogenesis are not fully understood. Using spatial metabolomics (chemical cartography), we sought to understand the localized impact of infection on the cardiac metabolome of mice chronically infected with two divergent T. cruzi strains. Our data showed chemical differences in localized cardiac regions upon chronic T. cruzi infection, indicating that parasite infection changes the host metabolome at select sites in chronic CD. These sites were distinct from the sites of highest parasite burden. In addition, we identified acylcarnitines and phosphocholines as discriminatory chemical families within each heart region, comparing infected and uninfected samples. Overall, our study indicated overall and positional metabolic differences common to infection with different T. cruzi strains, and identified select infection-modulated pathways. These results provide further insight into CD pathogenesis and demonstrate the advantage of a spatial perspective to understand infectious disease tropism.Author SummaryChagas disease (CD) is a tropical disease caused by the parasite Trypanosoma cruzi. CD originated in South America; however, there are now 7-8 million people infected worldwide due to population movements. CD is transmitted through a triatomine vector, organ transplants, blood transfusions and congenital transmission. It occurs in two stages, an acute stage (usually asymptomatic) and the chronic stage. Chronic stage CD presents with severe cardiac symptoms such as heart failure, localized aneurysms and cardiomyopathy. Unfortunately, what causes severe cardiac symptoms in some individuals in chronic CD is not fully understood. Therefore, we used liquid chromatography-tandem mass spectrometry to analyze the heart tissue of chronically T. cruzi-infected and uninfected mice, to understand the impact of infection on the tissue metabolome. We identified discriminatory small molecules related to T. cruzi infection. We also determined that regions with the highest parasite burden are distinct from the regions with the largest changes in overall metabolite profile; these locations of high metabolic perturbation provide a molecular mechanism to why localized cardiac symptoms occur in CD. Overall, our work gives insight to chronic cardiac CD symptom development and shapes a framework for novel treatment and biomarker development.


2012 ◽  
Vol 15 (3) ◽  
pp. 7-9 ◽  
Author(s):  
R Z Nurlygayanov ◽  
E R Syrtlanova

Objectives. To study the vitamin D levels in the period of minimal sun exposure in individuals older than 50 years residing in the Republic of Bashkortostan. Materials and methods. In the period of low insolation (March) in 188 people (68 men and 120 women) aged over 50 years (mean age 66,1 ± 0,75 years) residing in the city (Ufa) and rural areas of the Republic of Bashkortostan we investigated the levels of 25 (OH) D and parathyroid hormone (PTH) by the indirect ELISA. Level of 25 (OH) D 25-50 nmol/l were considered as mild vitamin D deficiency; 12,5-25 nmol/l-as moderate deficiency, and levels below 12.5 nmol/l - as a severe vitamin D deficiency. Results. The average level of 25 (OH) D was 33,55 ± 1,28 nmol/l; in urban area 43,48 ± 1,59 nmol/l; in rural area 21,25 ± 1,06 nmol/l; 36,29±2,43 nmol/l in men; 32,28± 1,47 nmol/l in women. The levels of vitamin D in the urban population were twice as high as in rural areas. A statistically significant difference by gender in terms of the levels of vitamin D was not found. We saw normal levels of vitamin D only in 18 % of subjects (in the city- 30 %, in the rural areas - 2 %), 43 % of people had a mild vitamin D deficiency (in the city - 59 %, in rural areas - 24 %), 33 % - a moderate deficiency of vitamin D (in the city -11 %, in the rural areas - 60 %), and 6 % of the patients had a severe vitamin D deficiency (in the city - 0 %, in rural areas -14 %). Moderate to severe vitamin D deficiency in rural areas was significantly more prevalent (74 %) than in urban (11 %). Gender was not found to be a risk factor for the development of vitamin D deficit in people over 50 years. There was a negative correlation between vitamin D levels and patient age (r=-0,356, p <0,001) and between levels of vitamin D andPTH (r =-0,313, p <0.001).


2012 ◽  
Vol 45 (6) ◽  
pp. 701-706 ◽  
Author(s):  
Antonio Fernando Rodrigues Lima ◽  
Veronica de Lourdes Sierpe Jeraldo ◽  
Maxwell Souza Silveira ◽  
Rubens Riscala Madi ◽  
Thiago Bicudo Krempel Santana ◽  
...  

INTRODUCTION: The present study identified the triatomines collected in intra and peri-domestic environments, observed the occurrence of Trypanosoma cruzi infection in triatomines and correlated this information with housing conditions and the fauna associated with the rural areas of the City of Itabaianinha, located in the State of Sergipe, Brazil. METHODS: Quarterly visits were conducted between March 2009 and March 2010, and the homes to be visited for the active search of insects were determined by random selection. In each housing unit, the insects were collected by a manual search with a metal clip and flashlight to inspect openings and cavities, with a collection time of one hour/home/individual. The Pirisa® dislodge chemical was used to force the insects to leave their ecotopes. Analysis of the intestinal contents of triatomines was performed in the laboratory to establish the presence of Trypanosomatidae. RESULTS: Of the 103 dwellings surveyed, 17.5% were infested with Panstrongylus megistus. The village of Mutuca exhibited the highest infestation rate (38.1%). All the villages with relevant infestation rates were situated in the northern area of the city. The highest percentage of vector infection was found in the village of Água Boa (56.5%). The rural dwellings were found to be primarily brick or wooden house with or without roughcast or plastered walls, and the outbuilding most frequently associated with triatomines was the chicken run. CONCLUSIONS: These results emphasise the need for broader vector control and surveillance and for educational campaigns in the context of the Chagas Disease Control Program.


2018 ◽  
Vol 51 (2) ◽  
pp. 190-197 ◽  
Author(s):  
Yannara Barbosa Nogueira Freitas ◽  
Celeste da Silva Freitas de Souza ◽  
Jamille Maia e Magalhães ◽  
Maressa Laíse Reginaldo de Sousa ◽  
Luiz Ney d’Escoffier ◽  
...  

Author(s):  
Carlos Eduardo Menezes Viana ◽  
Eduardo Arrais Rocha ◽  
Ana Rosa Pinto Quidute ◽  
Alanna Carla da Costa ◽  
Monica Coelho Andrade ◽  
...  

The Pharmaceutical Care Service of the Chagas Disease Research Laboratory (LPDC), in the State of Ceará, Brazil, treats patients with Chagas disease (CD), characterized as a chronic, neglected disease that requires full patient follow-up. Objective: determine the socioeconomic and demographic profile of patients with CD treated at the LPDC. A descriptive cross-sectional study was carried out, in which 507 patients were treated from January 2007 to May 2016. The variables collected were gender, age, place of birth, schooling, family income, type of health care, occupation, living conditions and habits. The profile of Trypanosoma cruzi infected individuals consisted of 51.1% males; mean age of 50.4 years; 26.6% were small farmers; 48.7% had not finished elementary school; 36.9% had a family income of one minimum wage; 77.7% did not have private health insurance and most of them came from rural areas. Regarding habits, 66.5% were nonsmokers and 70.0% were sedentary. Additionally, an improvement was noted in housing conditions, with most patients moving from wattle and daub huts, which were their first homes, to brick houses. We conclude that the analysis of the socioeconomic and demographic profile of patients treated at the LPDC showed that most of them come from a low socioeconomic population, where the demand for treatment has been occurring at a later age, further reinforcing the importance of the follow-up provided by the LPDC.KEY WORDS: Chagas disease; health services research; health profile; pharmaceutical care.


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