scholarly journals Milled legume grain as urease source for the ammonization of elephant grass hay

2017 ◽  
Vol 52 (12) ◽  
pp. 1268-1275 ◽  
Author(s):  
Leonardo Fiusa de Morais ◽  
João Carlos de Carvalho Almeida ◽  
Delci de Deus Nepomuceno ◽  
Mirton José Frota Morenz ◽  
Bárbara Maria Gomes de Melo ◽  
...  

Abstract: The objective of this work was to evaluate the use of soybean (Glycine max), pigeon pea (Cajanus cajan), and jack bean (Canavalia ensiformis) as urease sources for elephant grass (Pennisetum purpureum) hay ammoniated with urea. The experimental design was completely randomized in a double factorial arrangement with one additional treatment: 4 urease source levels x 3 urease sources + 1 control. Chemical-bromatological analyses and carbohydrate fractionation were performed in the hay, and cumulative gas production in vitro was determined. There were interactions between urease level and source for neutral detergent fiber and acid detergent fiber, in which 1 and 2% jack bean lowered acid detergent fiber values, and 2% jack bean and 3% soybean reduced lignin content. The addition of milled legume grains reduces fiber components and increases non protein nitrogen content in elephant grass hay ammoniated with urea. Adding 4% milled soybean increases gas production in the soluble fraction.

2020 ◽  
Vol 36 (5) ◽  
Author(s):  
Leonardo Fiusa De Morais ◽  
João Carlos de Carvalho Almeida ◽  
Delci de Deus Nepomuceno ◽  
Rafaela Scalise Xavier Freitas ◽  
Bárbara Maria Gomes De Melo ◽  
...  

The aim of this study was to evaluate the effect of ammoniation by urea on the nutritional value of elephant grass hay harvested after flowering. A completely randomized design, in double factorial designs with an additional treatment: 4 urea doses (2, 4, 6 and 8%) x 2 treatment periods (30 and 45 days) + 1 (Control) with four replicates each, was employed. Dry matter, ash, ether extract, neutral detergent fiber, acid detergent fiber, cellulose, lignin, neutral detergent fiber corrected for the ash and the protein, total nitrogen, acid detergent insoluble nitrogen, neutral detergent insoluble nitrogen, non-protein nitrogen, in vitro gas production and carbohydrates fractionation were analyzed. The treatments influenced the contents of DM, EE, NDF, ADF, lignin, cellulose, NDFap, fraction A + B1, fraction B2, fraction C, TN, NPN, ADIN, NDIN, L and Vf2. There was a positive linear effect of urea dose for NDF, ADIN, NDIN and L, positive linear effect on fraction A + B1, NT and NPN, and positive quadratic effect for Vf2, with absolute maximum point of 4.5%. Elephant grass hay harvested after flowering has its nutritional value improved, with a minimum dose of 4.5% urea on a dry matter basis.


2020 ◽  
Vol 7 (3) ◽  
pp. 179
Author(s):  
Badat Muwakhid ◽  
Usman Ali

ABSTRAK Pupuk cair yang memanfaatkan bahan organik seperti urin yang difermentasi dan zat pengatur tumbuh dapat memberikan tambahan unsur hara yang diperlukan rumput untuk tumbuh. Upaya perbaikan kualitas rumput gajah dapat dilakukan dengan pemberian pupuk daun “organik”, dengan memperhatikan dosis pemberiannya. Tujuan penelitian ini adalah menentukan dosis pemberian pupuk daun “organik” yang tepat untuk mendapatkan kualitas hijauan yang maksimal. Materi penelitian ini meliputi pupuk daun “organik” dan bibit rumput gajah dalam keadaan pols, dengan rata-rata panjang perpols adalah 10 cm. Penelitian menggunakan metode percobaan dengan menggunakan rancangan acak lengkap (RAL) dengan 4 perlakuan dan masing-masing perlakuan menggunakan 5 kali ulangan. Perlakuan yang diberikan adalah P0 (kontrol), P1 (5% pupuk daun “organik”), P2 (10% pupuk daun “organik”) dan P3 (15% pupuk daun “organik”). Hasil penelitian ini menunjukkan bahwa perlakuan pemberian dosis pupuk daun “organik” yang berbeda memperikan pengaruh yang sangat nyata (P<0,01) terhadap kandungan Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), selulosa, hemiselulosa, lignin, kecernaan in vitro bahan kering dan kecernaan in vitro bahan organik rumput gajah. Berdasarkan penelitian ini pemberian dosis 15% pupuk daun “organik” menunjukkan hasil yang paling baik.Kata kunci: komposisi kimiawi. pupuk daun organik, rumput gajah, , kecernaanABSTRACTLiquid fertilizers using organic ingredients such as bio urine can provide additional nutrients to optimize the grow of grass. Efforts to improve the quality of elephant grass can be done by providing this organic fertilizer by calculating the dosage given. The purpose of this study was to determine the appropriate dosage of “organic” leaf fertilizers to obtain maximum forage quality. The research material used were “organic” leaf fertilizers and elephant grass seedlings in a state of pols, with an average length of each pols is 10 cm. The research method is experiment using a completely randomized design (CRD) which consisted of 4 treatments and 5 repetitions. The treatments given in this study were P0 (control), P1 (5% “organic” leaf fertilizers), P2 (10% “organic” leaf fertilizers) and P3 (15% “organic” leaf fertilizers). The results of this study indicate that the implementation of different dosages of “organic” leaf fertilizers had significant effect (P <0.01) on the content of Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), cellulose, hemicellulose, lignin, dry matter digestibility (in vitro) and organic matter digestibility (in vitro) of elephant grass. Based on this study, 15% dose of “organic” leaf fertilizers showed the best results.Keywords: chemical compounds, digestibility, elephant grass, organic leaf fertilizers


2018 ◽  
Vol 19 (3) ◽  
pp. 277-286 ◽  
Author(s):  
Cintia Mirely de Araujo ◽  
Fernanda Gomes Bezerra da Silva ◽  
Daniela Pionório Vilaronga Castro ◽  
Daniel Ribeiro Menezes ◽  
Mário Adriano Ávila Queiroz ◽  
...  

SUMMARY This study evaluated the influence of the propolis ethanolic extract (PEE) on gas production and in vitro degradability of sheep diets. Five experimental diets (treatments) were evaluated: without addition of PEE; 6 mL PEE; 12 mL PEE; 24 mL PEE and 36 mL PEE/kg concentrate. The experimental diet consisted of 50% elephant grass (Pennisetum purpureum) and 50% concentrate. There was a quadratic effect (P <0.05) for the volumes of total gas production (Vt), gases produced by the rapid degradation fractions (Vf1), and for in vitro degradability at 120 hours (Deg120), where the lowest values of Vt, Vf1 and Deg120, were found for the inclusion of 9.4 mL PEE/kg concentrate. Gas production by fermentation of the slow degradation fraction (Vf2) presented a mean of 25 mL/g DM (P <0.05). The colonization time of food particles (λ) significantly reduced (P<0.05) with increasing inclusion of PEE. Thus, it can be concluded that the PEE up to the inclusion of 9.4 mL/kg concentrate was efficient in inhibiting in vitro total gas production and from the fractions of rapid degradation by 9.9 and 15.3%, respectively, in addition to promoting a reduction of 5.3% in degradability after 120 h incubation in diets with a concentrate: forage ratio of 50:50. Thus, the inhibition in gas production was proportionally greater than the reduction of degradability.


2019 ◽  
Vol 40 (5Supl1) ◽  
pp. 2397
Author(s):  
Clésio dos Santos Costa ◽  
Rosane Cláudia Rodrigues ◽  
Ricardo Alves de Araújo ◽  
Francisco Naysson de Sousa Santos ◽  
Giovanne Oliveira Costa Sousa ◽  
...  

he aim of this study was to evaluate fermentation and chemical characteristics and the in situ degradability of sorghum silages enriched with dried Leucaena. The experiment was conducted as completely randomized design with four treatments (0 - control silage, silage without leucaena; 10.0, 20.0, or 30.0% inclusion of Leucaena) and five replicates, totaling 20 experimental units. The inclusion levels of leucaena influenced (P 0.05), with all silages having values above 90%. Gas loss decreased (P < 0.05) as the level of leucine increased in sorghum silage. A linear increase was observed for dry matter (P ? 0.0001) and crude protein (P = 0.0008) contents in response to Leucaena inclusion. There was a linear (P < 0.05) linear effect on neutral detergent fiber, hemicellulose and acid detergent fiber. As leucaena inclusion levels increased, for each 1% inclusion a reduction of 0.179 was observed; 0.059 and 0.119% for the values of neutral detergent fiber, hemicellulose and acid detergent fiber respectively. The inclusion of leucaena influenced (P < 0.05) the increase of silage lignin levels, probably due to the higher lignin content of the legume. The soluble fraction (a) of the silages increased along with the Leucaena inclusion levels, with highest values observed at the inclusion of 20 and 30% of the legume, respectively. Potential degradation (A) increased linearly with the levels of Leucaena added to the silage; the highest value for this variable was observed at 30% inclusion. Effective degradability (ED) increased up to the inclusion level of 20% (46.77%). Degradation rate (c) decreased markedly with the use of 30% Leucaena. The use of the forage part of Leucaena dried for three hours leads to reduced gas production, increased dry matter and crude protein contents, and decreased fiber contents. The degradability of dry matter increases up to the inclusion level of 20%.


2019 ◽  
Vol 40 (5) ◽  
pp. 2045 ◽  
Author(s):  
Flávio Pinto Monção ◽  
Marco Aurélio Moraes Soares Costa ◽  
João Paulo Sampaio Rigueria ◽  
Marielly Maria Almeida Moura ◽  
Vicente Ribeiro Rocha Júnior ◽  
...  

The objective was to evaluate the productivity, chemical composition, ruminal degradability of dry matter and digestibility of BRS capiaçu grass (Pennisetum purpureum Schum.) managed at five regrowth ages. A completely randomized design with ten replications was used in the BRS capiaçu elephant grass subjected to five cutting intervals (30, 60, 90, 120 and 150 days) in the summer, making a total of 50 plots with a useful area of 4 x 2 m. There were daily increases in the dry matter production in the order of 382 kg ha-1, with 49,859 kg ha-1 being produced at 150 regrowth days. The dry matter content and organic matter linearly increased (P < 0.01) at different regrowth ages. The crude protein content, in vitro dry matter digestibility and in vitro digestibility of neutral detergent fiber linearly reduced (P < 0.01) 0.037%, 0.196% and 0.256% per day, respectively. Potential degradability of dry matter decreased from 68.9% at 30 days to 44.7% at 150 regrowth days (0.194 percentage units per day). The rate of degradation of fraction B 'c' was not modified (P = 0.94), averaging 1.46% hour-1. In the cultivation of BRS capiaçu elephant grass in the summer season, in the northern region of Minas Gerais, the age for harvesting between 90 and 120 days of regrowth is recommended.


2010 ◽  
Vol 39 (3) ◽  
pp. 462-470 ◽  
Author(s):  
Jucilene Cavali ◽  
Odilon Gomes Pereira ◽  
Sebastião de Campos Valadares Filho ◽  
Marlos Oliveira Porto ◽  
Francisco Eden Paiva Fernandes ◽  
...  

The effects of different ratios of sugarcane and elephant grass (0:100, 25:75, 50:50, 75:25 and 100:0% of the natural basis) were assessed on the chemical composition and losses in silages treated with a bacterial inoculant, using laboratory silos. A 2 × 5 factorial arrangement (with and without inoculant and five elephant grass ratios) in a randomized blocks design with three replications was used. Interaction was observed in the sugarcane and elephant grass ratio × bacterial inoculant for crude protein (CP) and pH. The other variables were influenced only by the increasing proportions of elephant grass. The contents of dry matter, neutral detergent fiber corrected for ashes and protein, acid detergent fiber, insoluble protein in acid detergent fiber, hemicellulose and lignin in the silages increased linearly with the proportions of elephant grass. The water soluble carbohydrate contents and dry matter in vitro digestibility of the silages decreased linearly with the increase in the proportion of elephant grass. The mean value of ammonia nitrogen in relation to total N was 7.0% (% of DM). The CP values of the inoculated and non-inoculated silage fitted linear models. The highest CP content was observed in the silage treated with inoculant. The pH values of the silages, with and without inoculant, fitted quadratic and linear models, respectively. The lactic acid, propionic acid and butyric acid contents were not influenced by the elephant grass ratios, while the acetic acid content, for the non-inoculated silages, and ethanol decreased linearly with the increase in elephant grass. The ratio of 25% sugarcane and 75% elephant grass improves the nutritional value and increases the silage DM recovery, because of the lower effluent and gas productions. The homofermentative bacterial inoculant does not affect the sugarcane silage.


2021 ◽  
Vol 11 (4) ◽  
pp. 685-697
Author(s):  
Mohamed Rashid Salama Rashid ◽  
Mohamed Ahmed Hanafy ◽  
Mervat Sayed Hassan Youssef ◽  
Harry Archimède ◽  
Sobhy Mohamed Abdallah Sallam ◽  
...  

Leucaena leaves and rejected green banana fruits can be promising to cope with feed gaps in arid and semi-arid Mediterranean regions. The present study evaluated the feeding value and secondary active compounds of Leucaena leaves and rejected green banana fruits for ruminants using a semi-automated gas production (GP) system. Comparisons were made with the traditional feeds as alfalfa, and Dichanthium spp. grass hay. Analysis of HPLC was performed for the feed ingredients to characterize the main phenolic components. The in vitro evaluation was carried out for the experimental feed ingredients and diets. Four diets were formulated as the first diet consisted of alfalfa and grass hay at a ratio of 35:65 (AG), the second diet composed of alfalfa, grass hay, and green banana fruits at a ratio of 35:55:10 (AGB), third and fourth diets were prepared by replacing alfalfa in AG and AGB with Leucaena leaves to be LG and LGB, respectively. Leucaena leaves showed a high content of valuable phenolic components that have antioxidant and anti-inflammatory properties, such as gallic acid, ellagic acid, and naringenin. Moreover, Leucaena leaves and diet had higher crude protein, total phenols, and total tannins than alfalfa, which was reflected on the chemical composition of diets, and recorded the lowest total accumulative GP at 24 hours leading to low CH4 and CO2 production. Banana fruits recorded the lowest ruminal pH, ammonia concentration, and degraded neutral detergent fiber, compared to other feed ingredients, while it had the highest GP and degraded organic matter. Therefore, it is highly recommended to use Leucaena leaves in animals’ diets with/without rejected green banana fruits as an alternative feed resource with potential environmental and animal health benefits.


Author(s):  
Henrique Guimarães De Favare ◽  
Joadil Gonçalves De Abreu ◽  
Livia Vieira De Barros ◽  
Felipe Gomes Da Silva ◽  
Luis Miguel Mendes Ferreira ◽  
...  

Aimed to evaluate the bromatological composition of different genotypes of elephant grass (Pennisetum purpureum Schum.) to energy production through combustion. The experimental design was a randomized block with 3 repetition and the treatments arranged in a subdivided plots scheme, considering as a plots the thirteen genotypes and harvests (dry and rainy) as subplots. The genotypes evaluated were Cubano Pinda, Porto Rico, Vrukwona, Piracicaba 241, Cuba 116, Taiwan A-25, Mecker, Napier, Canará, Guaçu, Cameroon, CNPGL 93-41-1 and CNPGL 91-25-1 clones. The experiment lasted two consecutive years with harvests made every 6 months, with a harvest in the dry season (September) and another one in the rainy season (March), totaling 4 harvests. For dry matter content analysis, three tillers were selected at random and dried in an oven at 55ºC until reaching a constant mass. For biomass quality analysis, the samples were ground in Willey type mills with 1 mm sieves, submitted to bromatological analysis to determine the neutral detergent fiber, acid detergent fiber, hemicellulose, volatile materials, and fixed carbon content. Higher levels of dry matter (greater than 44.4%), acid detergent fiber (greater than 44.8%), volatile matter (greater than 94.3%) and higher calorific value (greater than 3,450 kcal kg-1) occur in the dry period of the year and in genotypes Mercker, Piracicaba 241, Guaçu and BRS Canará genotypes.


Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 109
Author(s):  
Sukruthai Sommai ◽  
Anusorn Cherdthong ◽  
Chanon Suntara ◽  
Sarong So ◽  
Metha Wanapat ◽  
...  

Two experiments were conducted under this study: Experiment 1 was to study production yield, chemical composition, and in vitro degradability of Brazilian spinach (Alternanthera sissoo; BS) leaf and leaf + leaf-stalk at various maturity ages of 15, 30, 45, and 60 days after plantation and regrowth and Experiment 2 was to evaluate the effect of flavonoid extract from BS leaf and leaf + leaf-stalk and dietary ratios on ruminal gas production, fermentation characteristics, and in vitro degradability. Experiment 1 showed that maturity ages after planting and regrowth increased, the yield significantly increased. Increasing maturity ages significantly (p < 0.05) increased neutral detergent fiber and acid detergent fiber content and decreased crude protein content, total flavonoid (TF) content, and degradability for both leaf and leaf + leaf-stalk. Maturity ages from 15 to 30 days after plantation and regrowth resulted (p < 0.05) the highest TF content and degradability for both leaf and leaf + leaf-stalk. Thus, BS leaf and leaf + leaf-stalk samples from 15 to 30 days of age were used for flavonoid extraction and used in the Experiment 2. Experiment 2 was conducted according to a 3 × 5 factorial experiment. Three roughage to concentrate (R:C) ratios at 50:50, 40:60, and 30:70 were used, and five levels of flavonoid extract (FE) at 0, 10, 20, 30, and 40 mg of substrate dry matter (DM) were supplemented. Experiment 2 showed that R:C ratio and FE had an interaction effect only on acetate to propionate ratio. Varying R:C ratios significantly increased (p < 0.05) in vitro DM degradability, total volatile fatty acids (VFA), and propionate (C3) concentration. FE supplementation linearly (p < 0.05) increased total VFA and C3 concentration and decreased methane production and protozoal population. This study could conclude that FE from BS could effectively modulate ruminal fermentation and decrease methane production. However, in vivo study needs to elucidate in order to validate the present results.


2020 ◽  
Vol 8 (2) ◽  
pp. 147-152
Author(s):  
Juliana Caroline Santos Santana ◽  
Jucileia Aparecida da Silva Morais ◽  
Gelson Dos Santos Difante ◽  
Luís Carlos Vinhas Ítavo ◽  
Antonio Leandro Chaves Gurgel ◽  
...  

This study examined fermentation rates and kinetics of sheep rations based on combinations of elephant grass hay, gliricidia (Gliricidia sepium) hay or silage, soybean meal and corn meal using in vitro techniques. Three rations were prepared, namely: Control (elephant grass hay + soybean meal + corn meal); gliricidia hay (elephant grass hay + soybean meal + corn meal + gliricidia hay); and gliricidia silage (elephant grass hay + soybean meal + corn meal + gliricidia silage). A fixed ratio of roughage:concentrate of 55:45 was maintained for all rations, which were isocaloric and designed to support sheep gains of 200 g/day. The gliricidia replaced 57.6% of the soybean meal in the rations containing gliricidia and 81.8% of the elephant grass hay. Fermentation rates and kinetics, in vitro dry matter digestibility (IVDMD) and degradability of the rations were evaluated. Rations containing gliricidia as both hay and silage had higher (P<0.05) IVDMD than the Control ration (67.8 and 66.2 vs. 59.8%). The degradability of the ration containing gliricidia hay was higher (P<0.05) than that of the gliricidia silage ration (57.8 vs. 50.5%), whereas the Control showed an intermediate value (54.4%). The ration containing gliricidia hay and the Control produced more gas in the first 24 h than the ration containing gliricidia silage, and the gliricidia hay ration showed the shortest colonization time. Peak gas production occurred for the ration with gliricidia silage later than for the other rations. The study showed that substituting soybean meal with preserved gliricidia can result in higher digestibility of sheep rations. Feeding studies with animals are now warranted to verify these laboratory findings.


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