scholarly journals Paratuberculosis in a dairy Gyr herd in the State of Paraíba, Brazil

2009 ◽  
Vol 29 (9) ◽  
pp. 703-706 ◽  
Author(s):  
Pedro M.P.C. Mota ◽  
Prhiscylla S. Pires ◽  
Ronnie A. de Assis ◽  
Felipe M. Salvarani ◽  
Ricardo de M.H. Leite ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Egg Yolk ◽  
Giant Cells ◽  
Intestinal Wall ◽  
The State ◽  
Northeastern Brazil ◽  
Growth Time ◽  
Zebu Cattle ◽  
Fecal Samples ◽  
Bacterial Morphology

This paper describes the clinical, pathological, and microbiologic aspects of paratuberculosis (Johne's disease) in a dairy Gyr herd in the State of Paraíba, northeastern Brazil. An eight years old cow with chronic unresponsive diarrhea was clinically examined and euthanized for pathological evaluation. Fecal samples from all 160 animals over 12 months of age from the herd were collected for isolation of Mycobacterium avium subsp. paratuberculosis. Clinically, the index case cow was severely dehydrated, cachectic, with profuse mucous diarrhea. The main post-mortem findings were emaciation and thickened intestinal wall. Microscopically, the intestinal lamina propria and submucosa were infiltrated by macrophages, epithelioid cells, and Langhans giant cells with numerous alcohol-acid resistant bacilli in the cytoplasm. Two fecal samples displayed growth in slants of Herrold's egg-yolk agar supplemented with mycobactin J, 150 days after incubation. No growth was noticed in slants without mycobactin J. Microscopic examination of the isolated microorganisms stained by Ziehl-Neelsen revealed considerable amounts of alcohol-acid resistant bacilli, morphologically compatible with Mycobacterium spp. Based on the clinical signs, gross and histological lesions, growth time, bacterial morphology in Ziehl-Neelsen staining, and dependence of mycobactin J, the first diagnosis of paratuberculosis in Zebu cattle was made.

scholarly journals Diversity of enteric coccidia in pigs from the Paraíba Semiarid Region of Northeastern Brazil

2020 ◽  
Vol 29 (4) ◽  
Author(s):  
Hosaneide Gomes de Araújo ◽  
Juliana Trajano da Silva ◽  
Wlysse Ferreira Sarmento ◽  
Samara dos Santos Silva ◽  
Roberto Alves Bezerra ◽  
...  
Keyword(s):  
Morphological Analysis ◽  
The State ◽  
Northeastern Brazil ◽  
Semiarid Region ◽  
Mixed Infections ◽  
Family Farming ◽  
Fecal Samples ◽  
Hemorrhagic Enteritis ◽  
Prevalent Species ◽  
Genus Eimeria

Abstract There is great diversity in swine coccidia, which are responsible for causing intestinal disorders ranging from sporadic diarrhea to severe cases of hemorrhagic enteritis. Thus, determining the species of coccidia that affect the animals of a region and associating them with the characteristics of the farms become extremely important. The objective of this study was to determine the prevalence of coccidia parasites in pigs reared in a family farming production system in the Semiarid Region of the State of Paraíba, Northeast Brazil. Fecal samples for analysis were collected from 187 pigs on 51 farms. For morphological analysis, 1,590 sporulated oocysts were used. The prevalence of oocysts in fecal samples was 56.6% (106/187). The most prevalent species were Eimeria suis (21.9%), followed by Eimeria neodebliecki (16.6%), Eimeria perminuta (14.9%), Eimeria polita (12.8%), Eimeria debliecki (10.6%), Eimeria porci (10.1%), Cystoisospora suis (3.7%), Eimeria scabra (1.6%) and Eimeria cerdonis (0.5%). It can be concluded that pigs from the Semiarid Region of the State of Paraíba were parasitized by a diversity of coccidia species, mainly of the genus Eimeria, and predominantly presented with mixed infections occurring in the subclinical form.

scholarly journals Poisoning by Poiretia punctata in cattle and sheep

2014 ◽  
Vol 34 (10) ◽  
pp. 963-966 ◽  
Author(s):  
Eduardo M. Nascimento ◽  
Rosane M.T. Medeiros ◽  
Stephen T. Lee ◽  
Franklin Riet-Correa
Keyword(s):  
Body Weight ◽  
Clinical Signs ◽  
The State ◽  
Northeastern Brazil ◽  
Cyanogenic Glycosides ◽  
Negative Results ◽  
Clinical Observations ◽  
Plant Collection ◽  
Toxic Plant ◽  
Cattle And Sheep

Poiretia punctata (Willd.) Desv. was associated with cattle and sheep poisoning on nine farms in the State of Sergipe, northeastern Brazil. The animals were found dead or died later after showing clinical signs for up to 18 hours. Two sheep that ingested 40g/kg body weight (g/kg) of fresh P. punctata died three and eight hours after ingestion, respectively. Another sheep that ingested 40g/kg five days after plant collection showed mild clinical signs and recovered after 24 hours. Two sheep that received 20g/kg and another that ingested three daily doses of 20g/kg showed clinical signs, but recovered. Two cattle that ingested 20g/kg of the fresh plant exhibited clinical signs and recovered. The clinical observations of poisoning were depression, ataxia, loss of equilibrium, broad-based stance, head down, falls, mandibular trismus, opisthotonous, nystagmus, and recumbence. Significant gross and histologic lesions were not observed. Samples of P. punctata were analyzed for nitrates, cyanogenic glycosides, and sodium monofluouracetate with negative results. It is concluded that P. punctata is a toxic plant that caused death in cattle and sheep in the State of Sergipe.

scholarly journals Trombiculiasis in domestic goats and humans in the state of Maranhão, Brazil

2017 ◽  
Vol 26 (1) ◽  
pp. 104-109 ◽  
Author(s):  
João Luiz Horácio Faccini ◽  
Ana Clara Gomes Santos ◽  
Sandra Batista Santos ◽  
Fernando de Castro Jacinavicius ◽  
Ricardo Bassini-Silva ◽  
...  
Keyword(s):  
Rainy Season ◽  
Family Members ◽  
Clinical Signs ◽  
The State ◽  
Northeastern Brazil ◽  
First Report ◽  
The Family

Abstract Trombiculiasis is an infestation caused by larval mites (chiggers) of the family Trombiculidae. Here, we provide the first report on parasitism by the chigger species Eutrombicula alfreddugesi (Oudemans) and Eutrombicula batatas (Linnaeus) in goats and humans on farms in the state of Maranhão, northeastern Brazil. Severe itching and dermatitis caused by the chiggers' bites were seen. From a total of 779 examined goats, 214 of them showed clinical signs of infestation, as well as family members of three farms of the region. Most of the cases occurred during the rainy season, from March to September.

scholarly journals Equine coital exanthema caused by equid alphaherpesvirus 3: a report of an outbreak in northeastern Brazil

2021 ◽  
Vol 41 ◽  
Author(s):  
Givaldo B. Silva Filho ◽  
Hisadora A.S.C Bom ◽  
Silvio M.C. Fonseca ◽  
Érica A. Costa ◽  
Beatriz S.A.S. Santos ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Oral Cavity ◽  
Molecular Diagnosis ◽  
Clinical Signs ◽  
Rio Grande ◽  
The State ◽  
Northeastern Brazil ◽  
Semiarid Region ◽  
Blood Samples ◽  
Northeastern Region

ABSTRACT: Equine coital exanthema is a venereal infectious disease poorly reported in horses in Brazil and was never described in the northeastern region of the country. This work aims to describe the clinical and pathological aspects of an outbreak of equine coital exanthema caused by equid alphaherpesvirus 3, occurred in a herd of horses at the semiarid region of the State of Rio Grande do Norte. Main clinical signs consisted of anorexia, hiporexia, fibrinous or purulent secretion in the penis mucosa and vagina. Two mares presented mild to minimal lesions that consisted of scars in the mucosa of the vagina and in the perivulvar region. In a stallion the disease consisted of severe, multifocal, umbilicated-exanthematous ulcers of approximately 1cm in diameter on the penis mucosa. Other areas where ulcers and crusts were focally observed included the skin of the scrotum and on the lips and mucocutaneous junctions of the oral cavity. Histologically, the main lesion consisted of multifocal severe ulcerative and fibrinous necrotizing balanoposthitis and mild multifocal necrotizing, lymphocytic dermatitis in the lips and scrotum. The equide alphaherpesvirus 3 DNA was amplified in blood samples and penis mucosa using the PCR technique. This is the first report of molecular diagnosis of equine coital exanthema affecting horses in northeastern Brazil. Further studies should be carried out in order to investigate the epidemiology and the importance of this herpetic disease in the country.

scholarly journals Detection and molecular characterization of Trypanosoma (Duttonella) vivax in dairy cattle in the state of Sergipe, northeastern Brazil

2017 ◽  
Vol 26 (4) ◽  
pp. 516-520 ◽  
Author(s):  
Osires Lustosa Eloi Vieira ◽  
Lucia Oliveira de Macedo ◽  
Marcos Antônio Bezerra Santos ◽  
José Augusto Bastos Afonso Silva ◽  
Carla Lopes de Mendonça ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Clinical Signs ◽  
Buffy Coat ◽  
Feedlot Cattle ◽  
The State ◽  
Northeastern Brazil ◽  
Economic Losses ◽  
Blood Smears ◽  
Cattle Herds

Abstract Trypanosoma (Duttonella) vivax is an important cause of economic losses among feedlot cattle. These losses are related to the morbidity, mortality, reproductive issues and decreased production. It is known that the clinical signs observed in infections by this protozoon are similar to other hemoparasitosis, which difficult the diagnosis. Therefore, the aim of this study was to detect and molecularly characterize an outbreak of trypanosomiasis caused by T. (D.) vivax in dairy cattle in the municipality of São Miguel Aleixo, state of Sergipe, Brazil. Blood samples from cattle (n = 15) presenting clinical signs compatible with trypanosomiasis were collected and parasitological and molecular evaluated. Among the samples analyzed, 34% (5/15) were positive from blood smears, 60% (9/15) from the buffy coat method and 80% (12/15) from the molecular method. The DNA sequence obtained (659 bp) showed 99% similarity to T. (D.) vivax sequences that are available in the GenBank database. The presence of this protozoon in cattle herds is a problem for producers. Diagnosing trypanosomiasis is problematic because its evolution is similar to that of other parasitic blood diseases. In addition, this is the first report of infection by T. (D.) vivax in cattle in the state of Sergipe, northeastern Brazil.

scholarly journals Long-Term Survival ofMycobacterium aviumsubsp.paratuberculosisin Fecal Samples Obtained from Naturally Infected Cows and Stored at −18°C and −70°C

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Eran A. Raizman ◽  
L. A. Espejo ◽  
S. J. Wells
Keyword(s):  
Mixed Model ◽  
Bacterial Load ◽  
Clinical Signs ◽  
Egg Yolk ◽  
Median Number ◽  
Term Survival ◽  
Fecal Samples ◽  
Effects Of Temperature ◽  
Serum Elisa

The objective was to evaluate the survival of Mycobacterium paratuberculosis (Map) in naturally infected dairy cows feces under long periods of freezing at −18°C and −70°C. Samples were collected from cows previously tested positive with serum ELISA or fecal culture, or with clinical signs of Johne's disease. Samples were stored at −18°C and/or −70°C and recultured in Herrold's egg yolk media every 3–6 months. A proportional odds mixed model was used for data analysis. Sixty nine fecal samples were stored for different periods between September 2002 and January 2005. Of these, 45 (65%) were stored at −18°C and 24 (35%) at −70°C. Average number of days between repeated culture dates was 98 and 84 for −18°C and −70°C, respectively. Median number of repeated cultures was 6 and 4 for samples stored at −18°C and −70°C, respectively. After adjusting for initial sample bacterial load, the effects of temperature or number of thawing and refreezing cycles on Map viability were not significant. The probability that a sample decreases from high to moderate-low bacterial load and from moderate-low to negative bacterial load was 13.5% per month. Although this study found gradual reduction of Map concentration in stored fecal samples through time, overall survival in −18°C can ease fecal samples management in laboratories with low-processing capacity or lack of −70°C freezer.

scholarly journals Poisoning by Brunfelsia uniflora in sheep and donkeys

2018 ◽  
Vol 30 (3) ◽  
pp. 476-478
Author(s):  
Gustavo W. Mello ◽  
Franklin Riet-Correa ◽  
Maria C. Batista ◽  
Ciro J. Carvalho ◽  
Alexandre C. Dias ◽  
...  
Keyword(s):  
Body Weight ◽  
Rainy Season ◽  
Clinical Signs ◽  
The State ◽  
The Other ◽  
Northeastern Brazil

Farmers in the State of Piauí in northeastern Brazil reported nervous signs in ruminants and donkeys after ingestion of Brunfelsia uniflora at the start of the rainy season when the plant is flowering. Leaves of the plant, collected at the start or at the end of the rainy season, were administered in single doses of 5–20 g/kg body weight to 8 sheep and 3 donkeys. Two sheep and 1 donkey that ingested 10 g/kg of the plant in November at the start of the rainy season, when the plant was flowering, developed severe convulsions and diarrhea. One sheep was euthanized and autopsied, and no significant lesions were found. The other sheep and the donkey recovered. Four sheep and one donkey that ingested 10 or 20 g/kg of leaves collected in April, at the end of the rainy season, did not show clinical signs. One donkey that ingested 5 g/kg of leaves collected in November developed diarrhea and recovered. These results demonstrate the toxicity of B. uniflora for livestock and suggest that the plant is toxic at these doses only during the start of the rainy season.

A Synopsis of Dicliptera (Acanthaceae) in Brazil, with the Description of Two New Species

2020 ◽  
Vol 45 (2) ◽  
pp. 340-348
Author(s):  
James Lucas da Costa-Lima ◽  
Earl Celestino de Oliveira Chagas
Keyword(s):  
New Species ◽  
South America ◽  
The State ◽  
Northeastern Brazil ◽  
Mato Grosso ◽  
The North ◽  
New Synonyms ◽  
Two New Species ◽  
Mato Grosso Do Sul ◽  
A New Species

Abstract—A synopsis of Dicliptera (Acanthaceae) for Brazil is presented. Six species are recognized: Dicliptera ciliaris, D. sexangularis, and D. squarrosa, widely distributed in South America; D. purpurascens, which ranges from the North Region of Brazil (in the state of Acre) to eastern Bolivia; D. gracilirama, a new species from the Atlantic Forest of northeastern Brazil; and D. granchaquenha, a new species recorded in dry and semideciduous forests in Bolivia and western Brazil, in the state of Mato Grosso do Sul. Furthermore, we propose new synonyms and designate lectotypes for eleven names. An identification key to the six accepted Dicliptera species in Brazil is provided.

Spatiotemporal clustering, social inequities and the risk of leptospirosis in an endemic area of Brazil: a retrospective spatial modelling

2020 ◽  
Author(s):  
Katyucia O C de Souza ◽  
José Augusto P Góes ◽  
Matheus S Melo ◽  
Paula M G Leite ◽  
Lucas A Andrade ◽  
...  
Keyword(s):  
Urban Areas ◽  
Spatial Regression ◽  
Temporal Trends ◽  
Spatial Dynamics ◽  
Secondary Data ◽  
The State ◽  
Scan Statistics ◽  
Northeastern Brazil ◽  
Metropolitan Region ◽  
Social Inequities

Abstract Background Leptospirosis is an endemic disease in Brazil of social and economic relevance related to behavioural and socioenvironmental factors. This study aimed to analyse the spatiotemporal distribution of the incidence of leptospirosis and its association with social determinants in health in a state of northeastern Brazil. Methods An ecological study of temporal series with techniques of spatial analysis using secondary data of the cases of leptospirosis notified in the Information System of Notifiable Diseases of the state of Sergipe (2008–2017) was conducted. The analysis of temporal trends was performed using Poisson regression. Spatial analyses were performed using the Moran index, the local empirical Bayesian model, scan statistics and spatial regression. Results The incidence rate decreased from 3.66 to 1.44 cases per 100 000 inhabitants in 2008 and 2017, respectively. Leptospirosis was associated with social inequities, mostly affecting males aged 20–49 y living in urban areas. The space-time scan indicated the formation of a risk cluster in municipalities in the metropolitan region of the state. Conclusions The data indicated the persistence of leptospirosis transmission, maintaining a pattern of high endemicity in some municipalities associated with social inequities. The study showed the temporal and spatial dynamics of the disease to better target specific actions for prevention and control.

Porcine teschovirus, sapelovirus, and enterovirus in Swiss pigs: multiplex RT-PCR investigation of viral frequencies and disease association

2021 ◽  
pp. 104063872110258
Author(s):  
Tamara Stäubli ◽  
Charlotte I. Rickli ◽  
Paul R. Torgerson ◽  
Cornel Fraefel ◽  
Julia Lechmann
Keyword(s):  
Clinical Signs ◽  
Virus Detection ◽  
Common Finding ◽  
Fecal Samples ◽  
High Frequencies ◽  
Reverse Transcription Pcr ◽  
Suckling Piglets ◽  
Porcine Teschovirus ◽  
Fattening Pigs ◽  
Pathology Data

Porcine teschovirus (PTV), sapelovirus (PSV-A), and enterovirus (EV-G) are enteric viruses that can infect pigs and wild boars worldwide. The viruses have been associated with several diseases, primarily gastrointestinal, neurologic, reproductive, and respiratory disorders, but also with subclinical infections. However, for most serotypes, proof of a causal relationship between viral infection and clinical signs is still lacking. In Switzerland, there has been limited investigation of the occurrence of the 3 viruses. We used a modified multiplex reverse-transcription PCR protocol to study the distribution of the viruses in Swiss pigs by testing 363 fecal, brain, and placental or abortion samples from 282 healthy and diseased animals. We did not detect the 3 viruses in 94 placental or abortion samples or in 31 brain samples from healthy pigs. In brain tissue of 81 diseased pigs, we detected 5 PSV-A and 4 EV-G positive samples. In contrast, all 3 viruses were detected at high frequencies in fecal samples of both healthy and diseased pigs. In healthy animals, PTV was detected in 47%, PSV-A in 51%, and EV-G in 70% of the 76 samples; in diseased animals, frequencies in the 81 samples were 54%, 64%, and 68%, respectively. The viruses were detected more frequently in fecal samples from weaned and fattening pigs compared to suckling piglets and sows. Co-detections of all 3 viruses were the most common finding. Based on clinical and pathology data, statistical analysis yielded no evidence for an association of virus detection and disease. Further research is required to determine if pathogenicity is linked to specific serotypes of these viruses.

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