scholarly journals ANTIFUNGAL POTENTIAL OF A BRASSICACEOUS WEED Sisymbrium irio AGAINST Macrophomina phaseolina

2017 ◽  
Vol 35 (0) ◽  
Author(s):  
A. JAVAID ◽  
L. AFZAL ◽  
A. SHOAIB
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Fungal Pathogen ◽  
Fungal Biomass ◽  
Macrophomina Phaseolina ◽  
Root Extract ◽  
Root Extracts ◽  
Methanolic Extracts ◽  
Antifungal Potential ◽  
Sisymbrium Irio

ABSTRACT Macrophomina phaseolina, a soil-borne plant pathogen, has the ability to cause diseases in about 500 plant species. Unfortunately, so far no registered fungicide is available against this fungal pathogen. In the present study, different extracts of Sisymbrium irio, a weed of family Brassicaceae, were tested for evaluation of their antifungal activity against M. phaseolina. In screening bioassays, antifungal activity of methanolic extracts of 1% to 6% concentrations of different parts (leaf, stem, root and fruit) of the weed was assessed against the fungal pathogen. Methanolic leaf and root extracts significantly reduced fungal biomass up to 59% and 69% over control, respectively. Methanolic leaf and root extracts were further partitioned using four organic solvents namely n-hexane, chloroform, ethyl acetate and n-butanol in order of increasing polarity. Antifungal activity of different concentrations of these fractions (3.125, 6.25, …, 200 mg mL-1) was assessed against the pathogen. Chloroform and n-hexane fractions of methanolic leaf extract showed highly pronounced activity resulting in 35-75% and 15-87% reduction in fungal biomass over corresponding control treatments, respectively. Likewise, the highest concentration (200 mg mL-1) of chloroform, ethyl acetate and n-butanol fractions of methanolic root extract reduced fungal biomass by 75%, 70% and 87%, respectively. The present study concludes that chloroform and n-butanol fractions of methanolic leaf and root extracts of S. irio, respectively, contain potent antifungal constituents for management of M. phaseolina.

scholarly journals Antifungal Activity of Aerial Parts of Cenchrus pennisetiformis Against Fusarium oxysporum f. sp. lycopersici

2018 ◽  
Vol 36 (0) ◽  
Author(s):  
S. KHURSHID ◽  
A. JAVAID ◽  
A. SHOAIB ◽  
S. JAVED ◽  
U. QAISAR
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Fusarium Oxysporum ◽  
Fungal Pathogen ◽  
Fungal Biomass ◽  
Acid Ethyl Ester ◽  
Solanum Lycopersicum L ◽  
Aerial Parts ◽  
Antifungal Potential ◽  
Stem Extract

ABSTRACT: Antifungal potential of aerial parts of an allelopathic grass Cenchrus pennisetiformis (Hochst. & Steud.) Wipff. was evaluated against Fusarium oxysporum f. sp. lycopersici Snyder & Hansen, a fungal pathogen causing wilt disease in tomato (Solanum lycopersicum L.). Different concentrations (1% to 6%) of methanolic leaf, stem and inflorescence extract of the grass significantly reduced fungal biomass by 40-88%, 13-89%, and 26-76%, respectively. Methanolic shoot (leaf + stem) extract was fractionated using four organic solvents viz. n-hexane, chloroform, ethyl acetate and n-butanol. All the sub-fractions of methanolic shoot extract showed remarkable antifungal potential to variable extents. Different concentrations (1.56-200 mg mL-1) of ethyl acetate sub-fraction exhibited the best antifungal activity resulting in 49-100% suppression in the fungal biomass. GC-MS analysis of ethyl acetate sub-fraction showed the presence of 10 compounds. Phenol, 2,4-bis{1,1-dimethlethyl}- was the major compound (30.99%) followed by hexadecanoic acid, ethyl-ester (21.72%), benzofuran 2,3-dihydro (10.65%), 1-propanol-2-2-hydroxypropxy (10.60%) and 1-eicosene (8.32%).

scholarly journals Bioassays Guided Fractionation of Senna occidentalis for Identification of Natural Antifungal Constituents Against Macrophomina phaseolina

2017 ◽  
Vol 35 (0) ◽  
Author(s):  
A. JAVAID ◽  
H. QUDSIA ◽  
A. SHOAIB
Keyword(s):  
Antifungal Activity ◽  
Fungal Biomass ◽  
Leaf Extract ◽  
Plant Pathogens ◽  
Macrophomina Phaseolina ◽  
Initial Screening ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Fungal Plant Pathogens ◽  
Benzenedicarboxylic Acid

ABSTRACT Macrophomina phaseolina is one of the most destructive soil-borne fungal plant pathogens. In the present study, different aerial parts of Senna occidentalis were analyzed for their antifungal activity against M. phaseolina. In initial screening bioassays, 0.5-3.0% concentrations of methanolic extracts of fruit, leaf and stem were tested against M. phaseolina. The methanolic leaf extract showed the greatest activity causing up to 29% suppression in biomass production of M. phaseolina. Fractionation of leaf extract of S. occidentalis was carried out with four organic solvents. Bioassays with a range of concentrations (2.34-150 mg mL-1) of these sub-fractions revealed that the chloroform sub-fraction was the most effective, causing 93-98% reduction in the biomass of M. phaseolina, followed by 59-92% suppression in fungal biomass due to the n-hexane sub-fraction. The ethyl acetate and n-butanol sub-fractions were only effective at higher concentrations. GC-MS analysis of chloroform sub-fraction was performed to identify different compounds. Six compounds were identified in this fraction; 1,3-benzenedicarboxylic acid, bis(2-ethylhexyl) ester was the predominant compound (53.55%) followed by 9,10-dimethyltricyclo[4.2.1.1 (2,5)]decane-9,10-diol (22.68%), cyclohexanol, and 2-(2-hydroxy-2-propyl)-5-methyl (9.87%).

scholarly journals Bioassays Guided Fractionation of Ageratum conyzoides Extract for the Identification of Natural Antifungal Compounds against Macrophomina phaseolina

2021 ◽  
Vol 25 (04) ◽  
pp. 761-767
Author(s):  
Saira Banaras
Keyword(s):  
Methyl Ester ◽  
Ethyl Acetate ◽  
Fungal Biomass ◽  
Acid Methyl Ester ◽  
Macrophomina Phaseolina ◽  
Ageratum Conyzoides ◽  
Acid Methyl ◽  
Antifungal Potential ◽  
Stem Extract ◽  
Different Parts

Macrophomina phaseolina (Tassi) Goid. is a soil-borne fungal pathogen causing diseases in more than 500 plant species. The present study aimed to identify possible antifungal constituents in different parts of billygoat-weed (Ageratum conyzoides L.) through bioassays guided fractionation for the control of M. phaseolina. Extracts of different parts of the weed were made in methanol and antifungal bioassays were conducted using 1, 2, 3, 4 and 5% concentrations of the extract. Stem extract caused the highest inhibition in fungal biomass (20–83%) followed by leaf extract (16–67%). Methanolic stem extract was partitioned using four organic solvents namely n-hexane, chloroform, ethyl acetate and n-butanol. Bioassays carried out with different concentrations (3.125, 6.25, 12.5, 25, 50, 100 and 200 mg mL-1) of the sub-fractions of stem extract revealed the highest antifungal potential of chloroform sub-fraction with 56–93% reduction in the fungal biomass followed by n-butanol, ethyl acetate and n-hexane sub-fractions causing 24–76%, 7–75% and 5–70% reduction in fungal biomass over control, respectively. Chloroform sub-fraction with the highest antifungal potential was analyzed by GC-MS. Out of 10 compounds identified in this sub-fraction, 2H-1-benzopyran, 7-dimethoxy-2,2-dimethyl- (27.58%) was the most abundant followed by hexadecanoic acid, methyl ester (18.85%); 11-octadecenoic acid, methyl ester (15.28%) and 1,2-benzenedicarboxylic acid, mono(2-ethylhexyl) ester (10.88%), which could be responsible for antifungal activity. © 2021 Friends Science Publishers

scholarly journals Lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophomina phaseolina

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shabnam Javed ◽  
Zaid Mahmood ◽  
Khalid Mohammed Khan ◽  
Satyajit D. Sarker ◽  
Arshad Javaid ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Oleanolic Acid ◽  
Vanillic Acid ◽  
Fungal Biomass ◽  
Protocatechuic Acid ◽  
Methanolic Extract ◽  
Antifungal Compound ◽  
Chloroform Fraction ◽  
Lupeol Acetate

AbstractAntifungal activity of Monothecabuxifolia methanolic extract and its various fractions were assessed against Macrophominaphaseolina, a soil-borne fungal pathogen of more than 500 vegetal species as well as rare and emerging opportunistic human pathogen. Different concentrations of methanolic extract (3.125 to 200 mg mL−1) inhibited fungal biomass by 39–45%. Isolated n-hexane, chloroform and ethyl acetate fractions suppressed fungal biomass by 32–52%, 29–50% and 29–35%, respectively. Triterpenes lupeol and lupeol acetate (1, 2) were isolated from n-hexane while betulin, β-sitosterol, β-amyrin, oleanolic acid (3–6) were isolated from chloroform fraction. Vanillic acid, protocatechuic acid, kaempferol and quercetin (7–10) were isolated from the ethyl acetate fraction and identified using various spectroscopic techniques namely mass spectroscopy and NMR. Antifungal activity of different concentrations (0.0312 to 2 mg mL−1) of the isolated compounds was evaluated and compared with the activity of a broad spectrum fungicide mancozeb. Different concentrations of mencozeb reduced fungal biomass by 83–85%. Among the isolated compounds lupeol acetate (2) was found the highest antifungal against M.phaseolina followed by betulin (3), vanillic acid (7), protocatechuic acid (8), β-amyrin (5) and oleanolic acid (6) resulting in 79–81%, 77–79%, 74–79%, 67–72%, 68–71% and 68–71%, respectively. Rest of the compounds also showed considerable antifungal activity and reduced M.phaseolina biomass by 41–64%.

ANTIFUNGAL ACTIVITY OF LEAF EXTRACT OF Cannabis sativa AGAINST Aspergillus flavipes

2020 ◽  
Vol 27 (2) ◽  
pp. 447-453
Author(s):  
Iqra Haider Khan ◽  
Arshad Javaid
Keyword(s):  
Antifungal Activity ◽  
Fungal Biomass ◽  
Leaf Extract ◽  
Cannabis Sativa ◽  
Aqueous Fraction ◽  
Antifungal Effect ◽  
Butanol Fraction ◽  
Aspergillus Flavipes ◽  
Antifungal Potential ◽  
Laboratory Bioassays

Cannabis sativa L. is a medicinally important weed of family Cannabaceae generally grows along road-sides and waste-lends in Punjab, Pakistan. In the present study, antifungal effect of leaf extract of this weed was assessed against Aspergillus flavipes. Methanolic leaf extract of the weed was partitioned into five fractions using organic solvents of variable polarities. A range of concentrations (1.562 to 200 mg mL-1) of each fraction was used in laboratory bioassays. n-Butanol fraction showed the highest antifungal activity followed by chloroform and n-hexane fractions causing 68–82%, 52–82% and 42–82% decrease in biomass of A. flavipes. Ethyl acetate showed a moderate antifungal potential while aqueous fraction showed the least antifungal activity causing 47–76% and 38–73% reduction in fungal biomass, respectively. This study concludes that n-butanol fraction of leaf extract of C. sativa is highly effective in controlling growth of A. flavipes.

scholarly journals ANTIFUNGAL ACTIVITY OF Cirsium arvense EXTRACTS AGAINST PHYTOPATHOGENIC FUNGUS Macrophomina phaseolina

2017 ◽  
Vol 35 (0) ◽  
Author(s):  
S. BANARAS ◽  
A. JAVAID ◽  
A. SHOAIB ◽  
E. AHMED
Keyword(s):  
Antifungal Activity ◽  
Methyl Ester ◽  
Fungal Biomass ◽  
Acid Methyl Ester ◽  
Macrophomina Phaseolina ◽  
Phytopathogenic Fungus ◽  
Malt Extract ◽  
Leaf Extracts ◽  
Antifungal Activities ◽  
Acid Methyl

ABSTRACT Leaves, stems, roots and inflorescence of the asteraceous weed Circium arvense were extracted in methanol for two weeks. Methanol was evaporated in a rotary evaporator. Different concentrations (1, 2, 3, 4 and 5%) of methanolic extracts were prepared, and their antifungal activities were studied against Macrophomina phaseolina, using malt extract broth as growth medium. In general, extracts of all plant parts showed antifungal activities to variable extents. The highest antifungal activity occurred due to methanolic extract of leaves, followed by stem and root extracts, resulting in 10-74%, 6-57% and 11-39% reduction in fungal biomass over control, respectively. Inflorescence extract showed the least antifungal activity, resulting in 2-30% reduction in fungal biomass over control. There was a linear and inverse relationship between extract concentrations and fungal biomass for extracts of all the four parts. GC-MS analysis showed that there were 10 compounds in most effective methanolic leaf extract. Among these, 10-octadecanoic acid, methyl ester (26.442%), 2H-1-benzopyran, 6,7-dimethoxy-2-2-dimethyl (20.195%), hexadecanoic acid, methyl ester (15.752%) and 9,12-octadecadienoic acid (Z,Z)-, methyl ester (12.628%) were predominant compounds in the extract that may be responsible for antifungal activity. This study concludes that methanolic leaf extracts of C. arvense can be used for the management of M. phaseolina.

scholarly journals FUNGICIDAL POTENTIAL OF ALLELOPATHIC WEED Cenchrus pennisetiformis ON GROWTH OF Fusarium oxysporum f. sp. lycopersici UNDER CHROMIUM STRESS

2016 ◽  
Vol 34 (3) ◽  
pp. 453-463 ◽  
Author(s):  
S. KHURSHID ◽  
A. SHOAIB ◽  
A. JAVAID ◽  
U. QAISAR
Keyword(s):  
Fusarium Oxysporum ◽  
Contaminated Soils ◽  
Fungal Biomass ◽  
Negative Control ◽  
Wilt Disease ◽  
Root Extract ◽  
Malt Extract ◽  
Combined Application ◽  
Methanolic Extracts ◽  
Chromium Stress

ABSTRACT The present study was conducted to assess antifungal potential of the allelopathic grass Cenchrus pennisetiformis Hochst. & Steud. against the fungal plant pathogen Fusarium oxysporum f. sp. lycopersici (cause of tomato wilt disease) under chromium stress. Laboratory experiments were performed in 10 mL volume glass test tubes each containing 1.0 mL of malt extract broth with seven concentrations (0, 50, 100, 150, 200, 250, 300 and 350 ppm) of each of Cr(III) and Cr(VI), and two concentrations (5% and 6%) of methanolic leaf, stem or root extract of C. pennisetiformis. A metal + weed extract amended medium was inoculated with the pathogen and incubated for 7 days at 25 C. Different concentrations of Cr(III) and Cr(VI) proved equally inhibitory resulting in 10-84% and 18-89% reduction in fungal biomass, respectively. Methanolic leaf, stem and root extracts of the weed reduced fungal biomass by 12-25%, 14-23% and 46-50%, respectively, over negative control. In combined application of methanolic extracts of different parts of C. pennisetiformis and metal solutions, root extract in combination with either Cr(III) or Cr(VI) showed the highest inhibitory potential against the fungus followed by leaf and stem extracts. In combination with methanolic root, leaf and stem extracts, different concentrations of Cr(III) and Cr(VI) significantly reduced fungal biomass by 54-99%, 14-99% and 9-95%, respectively, over negative control. Such studies have not been carried out previously. Results of the present investigation suggest that F. oxysporum oxysporum f. sp. lycopersici, the cause of Fusarium wilt disease in tomato, can be managed by application of extracts (or alternatively biomass) of C. pennisetiformis in chromium contaminated soils.

scholarly journals Brine Shrimp Cytotoxic Activity of Morinda elliptica Leaves and Root Crude Extracts

2017 ◽  
Vol 7 (1) ◽  
pp. 43-46
Author(s):  
Henry Yusufu Wakawa ◽  
Fasihuddin Badrudin Ahmad ◽  
Zaini Bin Assim ◽  
Umaru Isaac John
Keyword(s):  
Mortality Rate ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Root Extract ◽  
Pharmacological Effects ◽  
Root Extracts ◽  
Brine Shrimp Lethality Assay ◽  
Morinda Elliptica ◽  
Bioactive Ingredients

This survey was conducted to test for in vivo Brine Shrimp Lethality Assay (BSLA) of Morinda elliptica leaves and root extract after successive maceration in four solvents (n-hexane, dichloromethane (DCM) ethyl acetate and methanol) and cytotoxicity was evaluated in terms of LC50 (lethality concentration). 10 nauplii were placed in different concentrations (in three replicates each) of the plant extracts, after 24 hours the surviving brine shrimp larvae were counted and LC50 was assessed. The result of the survey showed a concentration dependent increment in mortality rate of the brine shrimp nauplii and n-hexane and methanol fractions of the leaves and root extracts were more potent against the brine shrimp with LC50 values of 35.483 ppm and 39.259 ppm (μg/ml) respectively, whereas ethyl acetate of both the extracts exhibited lower potent activity with LC50 values 62.250 ppm and 102.250 ppm (μg/ml) in roots and leaves respectively. These findings indicated that bioactive ingredients are present in the plants that could account for its pharmacological effects.

scholarly journals Management of Ascochyta rabiei by Chenopodium album Extracts

2016 ◽  
Vol 34 (4) ◽  
pp. 675-680 ◽  
Author(s):  
A.Z. SHERAZI ◽  
K. JABEEN ◽  
S. IQBAL ◽  
Z. YOUSAF
Keyword(s):  
Antifungal Activity ◽  
Fungal Biomass ◽  
Chenopodium Album ◽  
Saturated Fatty Acids ◽  
Ascochyta Rabiei ◽  
Gas Chromatography Mass Spectrometry ◽  
Dilution Method ◽  
Antifungal Potential ◽  
Serial Dilution Method

ABSTRACT Chenopodium album, leaves were selected to evaluate their antifungal potential against Ascochyta rabiei causative agent for chickpea blight. Different concentrations of methanolic extract of C. album leaves i.e. 1%, 2.5%, 4%, 5.5% and 7% were tested against the target fungus A. rabiei. Maximum reduction in the test fungal biomass (68%) was observed in 7% concentration. This methanolic leaf extract was partitioned and n-butanol, chloroform, n-hexane, ethyl acetate fractions were isolated according to their polarity. In vitro antifungal activity of these fractions was studied by serial dilution method. n-hexane fraction exhibited the highest antifungal potential with 55% inhibition in test fungal biomass, so this fraction was selected for Gas chromatography mass spectrometry (GC-MS) analysis. Total thirteen compounds identified in this analysis belonged to class aromatic hydrocarbons, hydrocarbons, saturated fatty acids, aromatic carboxylic acid, siloxanes, phosphonates and cardiac glycosides. These compounds might be responsible for antifungal activity of C. album.

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