Laccase–MWCNT–chitosan biosensor—A new tool for total polyphenolic content evaluation from in vitro cultivated plants

Yerba maté extracts have in vitro antioxidant capacity attributed to the presence of polyphenolic compounds, mainly chlorogenic acids and dicaffeoylquinic acid derivatives. DPPH is one of the most used assays to measure the antioxidant capacity of pure compounds and plant extracts. It is difficult to compare the results between studies because this assay is applied in too many different conditions by the different research groups. Thus, in order to assess the antioxidant capacity of yerba maté extracts, the following procedure is proposed: 100 µL of an aqueous dilution of the extracts is mixed in duplicate with 3.0 mL of a DPPH 'work solution in absolute methanol (100 µM.L-1), with an incubation time of 120 minutes in darkness at 37 ± 1 °C, and then absorbance is read at 517 nm against absolute methanol. The results should be expressed as ascorbic acid equivalents or Trolox equivalents in mass percentage (g% dm, dry matter) in order to facilitate comparisons. The AOC of the ethanolic extracts ranged between 12.8 and 23.1 g TE % dm and from 9.1 to 16.4 g AAE % dm. The AOC determined by the DPPH assay proposed in the present study can be related to the total polyphenolic content determined by the Folin-Ciocalteu assay.

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IN VITRO ANTIOXIDANT CAPACITY, PHENOLIC, ASCORBIC ACID AND LYCOPENE CONTENT OF GUAVA (Psidium guajava L.) JUICES AND NECTARS

Boletim do Centro de Pesquisa de Processamento de Alimentos ◽

10.5380/cep.v27i2.16529 ◽

2009 ◽

Vol 27 (2) ◽

Cited By ~ 1

Author(s):

CARLOS ALEXANDRE KOGUISHI DE BRITO ◽

PRISCILA BECKER SIQUEIRA ◽

JANE CRISTINA DE SOUZA ◽

HELENA MARIA ANDRÉ BOLINI

Keyword(s):

Ascorbic Acid ◽

Antioxidant Capacity ◽

Ascorbic Acid Content ◽

Psidium Guajava ◽

Polyphenolic Compounds ◽

Polyphenolic Content ◽

Total Polyphenolic Content ◽

Lycopene Content ◽

Free Radicals Scavenging

capacity in vitro of three different brands of guava nectars and juices, through free radicals scavenging methods, 1,1-diphenyl-2-picrylhydrazine (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid (ABTS.+), and its correlation with the total polyphenolic content, total lycopene and ascorbic acid. Brands revealed statistical differences (p£ 0.05) in antioxidant capacity, ranging from 1.9 to 7.7 molTE/mL. Antioxidant capacity presented positive correlation for ascorbic acid content and polyphenolic compounds, being relatively low for the lycopene. Different process can influence the content of these compounds as well as interfere in their antioxidant capacity. The control of the production process is important to add value to guava products and fulfill the new tendency of the market.

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In Vitro Culture as a Stressful Factor Triggers Changes in Polyphenols, Flavonoids and Antioxidant Activity in Somatic Hybrids between Solanum tuberosum and S. bulbocastanum and their Respective Parents

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha45110463 ◽

2017 ◽

Vol 45 (1) ◽

pp. 75-81

Author(s):

Daniel CRUCERIU ◽

Imola MOLNAR ◽

Zorita DIACONEASA ◽

Adriana AURORI ◽

Carmen SOCACIU ◽

...

Keyword(s):

Antioxidant Activity ◽

In Vitro Culture ◽

Wild Species ◽

Somatic Hybrid ◽

Somatic Hybrids ◽

Flavonoid Content ◽

Polyphenolic Content ◽

Total Polyphenolic Content ◽

In Vitro Stress

Phenols, important secondary metabolites in plants, are responsible for specific defence mechanisms against abiotic stress, due to their strong antioxidant activity. Flavonoids, as part of phenolic group, are also involved in plant stress responses, being primarily responsible for photo-protection against UV solar radiation. Based on these premises, the plant response to optimized in vitro culture was evaluated, by quantifying the total polyphenolic content, the total flavonoid content and the antioxidant activity, both under in vitro and ex vitro conditions. Four closely related potato genotypes were analysed: the wild species Solanum bulbocatanum, S. tuberosum cv. ‘Rasant’ and two somatic hybrids between them. For all genotypes, both total polyphenolic content and antioxidant activity were increasing under optimized in vitro culture. The shoot responses were genotype dependent and the two somatic hybrids were intermediate between the parents both as morphology and reaction to in vitro stress. The somatic hybrid 1508/5, having morphology similar to potato, was reacting as the wild species, while the somatic hybrid 1508/2, with a similar morphology to the wild species was reacting as potato to in vitro stress. The somatic hybrid 1508/5 being also resistant to late blight is of interest for further use in pre-breeding. Total flavonoid content is decreasing under in vitro as compared to ex vitro conditions, UV-B radiation, the major trigger of flavonoid biosynthesis being absent in the fluorescent light. This study reveals the effect of in vitro culture on flavonoid content and details aspects of the biochemical parameters involved in plant in vitro stress.

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Electrochemical Methods and (Bio) Sensors for Rosmarinic Acid Investigation

Chemosensors ◽

10.3390/chemosensors8030074 ◽

2020 ◽

Vol 8 (3) ◽

pp. 74

Author(s):

Iulia Gabriela David ◽

Dana Elena Popa ◽

Mihaela Buleandră ◽

Mihaela Carmen Cheregi

Keyword(s):

Antioxidant Activity ◽

Dynamic Systems ◽

Phenolic Acid ◽

Rosmarinic Acid ◽

Electrochemical Methods ◽

Redox Active ◽

Polyphenolic Content ◽

Total Polyphenolic Content ◽

Electroactive Species ◽

Electrochemical Methods Of Analysis

Rosmarinic acid (RA) is an important bioactive phenolic acid with significant biochemical activities, including the antioxidant one. It is widely found in plants of the families Lamiaceae and Boraginaceae and has many uses in the food, pharmaceutical and cosmetics industries. RA is an electroactive species owing to the presence of the two catechol groups in its structure. Due to their inherent characteristics, such as sensitivity, selectivity, ease of operation and not too high costs, electrochemical methods of analysis are interesting tools for the assessment of redox-active compounds. Moreover, there is a good correlation between the redox potential of the analyte and its capability to donate electrons and, consequently, its antioxidant activity. Therefore, this paper presents a detailed overview of the electrochemical (bio)sensors and methods, in both stationary and dynamic systems, applied for RA investigation under different aspects. These comprise its antioxidant activity, its interaction with biological important molecules and the quantification of RA or total polyphenolic content in different samples.

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Disposable biosensor based on platinum nanoparticles-reduced graphene oxide-laccase biocomposite for the determination of total polyphenolic content

Talanta ◽

10.1016/j.talanta.2013.02.029 ◽

2013 ◽

Vol 110 ◽

pp. 164-170 ◽

Cited By ~ 45

Author(s):

Sandra A.V. Eremia ◽

Ioana Vasilescu ◽

Antonio Radoi ◽

Simona-Carmen Litescu ◽

Gabriel-Lucian Radu

Keyword(s):

Graphene Oxide ◽

Reduced Graphene Oxide ◽

Platinum Nanoparticles ◽

Reduced Graphene ◽

Polyphenolic Content ◽

Total Polyphenolic Content

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TOTAL POLYPHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF SILYBUM MARIANUM CULTURES GROWN ON DIFFERENT GROWTH REGULATORS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i4.16372 ◽

2017 ◽

Vol 9 (4) ◽

pp. 44 ◽

Cited By ~ 1

Author(s):

Hattem Mekky ◽

Samah El Sohafy ◽

Rasha Abu El-khair ◽

Amr El Hawiet

Keyword(s):

Antioxidant Activity ◽

Gallic Acid ◽

Growth Regulators ◽

Phenolic Content ◽

Wild Plant ◽

Total Phenolic ◽

Silybum Marianum ◽

Polyphenolic Content ◽

Trolox Equivalent ◽

Total Polyphenolic Content

Objective: The present study was designed to study the effect of supplementing Silybum marianum cultures with different combinations and concentrations of growth regulators on the total phenolic content and the antioxidant activity of the produced cultures.Methods: The total polyphenolic content of 23 extracts of calli was calculated as µg of gallic acid/mg of dry weight extract (DWE) using Folin–Ciocalteu reagent. The antioxidant activity was calculated as trolox equivalent antioxidant capacity using 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) assay for extracts containing more than 10 µg gallic acid/mg DWE.Results: Most of the combinations showed an increase in the total polyphenolic concentration compared to the wild plant. However, cultures grown on media supplemented with combinations of benzyl aminopurine (BAP) and gebberellic acid (Gb), adenine (Ad) and kinetin (Kin), BAP and indole acetic acid (IAA) possessed the highest total polyphenolic contents 20.5, 13.09 and 12.15 µg gallic acid/mg DWE, respectively. However, only (BAP+Gb), (BAP+1-Naphthaleneacetic acid (NAA), IAA, kin and (BAP+Ad) showed a significant increase in the antioxidant activity calculated as trolox equivalent with 2.65, 1.56, 1.56, 1.35 and 1.22 folds increase over the wild plant, respectively.Conclusion: The results obtained clearly indicated that changing the growth regulator system of Silybum marianum cultures serves as an easy and reliable method for increasing the total phenolic content and the antioxidant activity of cultures.

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Minerals and Total Polyphenolic Content of Some Vegetal Powders

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Animal Science and Biotechnologies ◽

10.15835/buasvmcn-asb:0002 ◽

2017 ◽

Vol 74 (2) ◽

pp. 185

Author(s):

Roxana E. TUFEANU ◽

Cecilia GEORGESCU ◽

Adina FRUM ◽

Mihaela A. TIȚA ◽

Ovidiu TIȚA

Keyword(s):

Inductively Coupled Plasma ◽

Nutritional Content ◽

Total Polyphenol ◽

Inductively Coupled ◽

Bioactive Properties ◽

Watermelon Rind ◽

Polyphenolic Content ◽

Calcium Phosphorus ◽

Total Polyphenolic Content ◽

Ultrasonic Water Bath

The total polyphenolic content and minerals were determined for chia seeds, Psyllium husks and watermelon rind powder. The minerals content was performed by using the Inductively Coupled Plasma Optical Emissions Spectrometer and Atomic Absorption Spectrometer, technique FIAS-Furnace (for Se). The sample with the highest content of polyphenols was chia (2.69 mg GAE/g s.) followed by the watermelon rind powder. Reduced amounts of polyphenols were found in the Psyllium husks. Also, the total polyphenol concentration increased with the increase of the extraction time on the ultrasonic water bath. Minerals analysis indicated that powders obtained from chia seeds and watermelon rind contained large amounts of potassium, calcium, phosphorus and magnesium. The most abundant mineral in the Psyllium husks powder was found potassium, followed by calcium. In conclusion, these powders can be used as ingredients for functional food and food supplements production due to the high nutritional content and bioactive properties.

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Iron nanoparticles on growth and acclimatization of Chrysanthemum morifolium Ramat. cv. "Jimba" in different culture systems

Vietnam Journal of Biotechnology ◽

10.15625/1811-4989/18/2/14646 ◽

2020 ◽

Vol 18 (2) ◽

pp. 307-319

Author(s):

Hoang Thanh Tung ◽

Truong Hoai Phong ◽

Phan Le Ha Nguyen ◽

Luong Thien Nghia ◽

Ha Thi My Ngan ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Enzyme ◽

Chlorophyll A ◽

Culture System ◽

Iron Nanoparticles ◽

Cultivated Plants ◽

Ms Medium ◽

Culture Systems ◽

Optimal Activity

In plant tissue culture, iron nanoparticles (FeNPs) was one of the first types of nano to be used in plants. Previous reports have identified the effect of FeNPs on many different plant species. In this study, FeNPs was used to replace Fe-EDTA in MS (Murashige, Skoog, 1962) medium to assess their effects on growth, chlorophyll (a, b and a+b) accumulation, antioxidant activity of ascorbate peroxidase (APX) and superoxide dismutase (SOD) enzymes, and acclimatization in greenhouse conditions in different culture systems (in vitro solid, in vitro hydroponic and microponic culture). The obtained results show that FeNPs added to MS medium was higher growth, chlorophyll (a, b and a+b) content, antioxidant activity of SOD and APX enzymes than Fe-EDTA in MS medium as control treatment. The effect of FeNPs are differences between culture systems. In vitro solid and microponic culture systems, the optimal concentration is 75 mM FeNPs and in vitro hydroponic culture system is 100 mM FeNPs. The optimal activity of the antioxidant enzyme SOD (35.04 U.mg−1 prot) obtained in the roots of cultured plants in microponic culture system; meanwhile, the optimal activity of the antioxidant enzyme APX (2.11 μmol.min−1.mg−1 prot) obtained in leaves cultivated in solid culture system. The plantlets derived from MS medium added FeNPs were transfered into greenhouse conditions, the microponic cultivated plants supplemented with FeNPs at a concentration of 100 mM gave the highest survival rate (94.67%). The results of this study showed that FeNPs can replace Fe-EDTA salt in MS medium, and iron deficiency in culture media will reduce chlorophyll content.

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Different expression pattern of flowering pathway genes contribute to male or female organ development during floral transition in the monoecious weed Ambrosia artemisiifolia L. (Asteraceae)

PeerJ ◽

10.7717/peerj.7421 ◽

2019 ◽

Vol 7 ◽

pp. e7421 ◽

Cited By ~ 1

Author(s):

Kinga Klára Mátyás ◽

Géza Hegedűs ◽

János Taller ◽

Eszter Farkas ◽

Kincső Decsi ◽

...

Keyword(s):

Genetic Regulation ◽

Ambrosia Artemisiifolia ◽

Cultivated Plants ◽

Gene Expressions ◽

Invasive Weed ◽

Male And Female ◽

Floral Organogenesis ◽

A Genome ◽

Reproduction Biology

The highly allergenic and invasive weed Ambrosia artemisiifolia L. is a monoecius plant with separated male and female flowers. The genetic regulation of floral morphogenesis is a less understood field in the reproduction biology of this species. Therefore the objective of this work was to investigate the genetic control of sex determination during floral organogenesis. To this end, we performed a genome-wide transcriptional profiling of vegetative and generative tissues during the plant development comparing wild-growing and in vitro cultivated plants. RNA-seq on Illumina NextSeq 500 platform with an integrative bioinformatics analysis indicated differences in 80 floral gene expressions depending on photoperiodic and endogenous initial signals. Sex specificity of genes was validated based on RT-qPCR experiments. We found 11 and 16 uniquely expressed genes in female and male transcriptomes that were responsible particularly to maintain fertility and against abiotic stress. High gene expression of homologous such as FD, FT, TFL1 and CAL, SOC1, AP1 were characteristic to male and female floral meristems during organogenesis. Homologues transcripts of LFY and FLC were not found in the investigated generative and vegetative tissues. The repression of AP1 by TFL1 homolog was demonstrated in male flowers resulting exclusive expression of AP2 and PI that controlled stamen and carpel formation in the generative phase. Alterations of male and female floral meristem differentiation were demonstrated under photoperiodic and hormonal condition changes by applying in vitro treatments.

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Peculiarities of in vitro rooting of some species of Carlina L. genus

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v20.767 ◽

1970 ◽

pp. 215-220

Author(s):

N. B. Kravets ◽

M. Z. Mosula ◽

N. M. Drobyk ◽

N. V. Tulaidan ◽

M. B. Cheterbok

Keyword(s):

Acid Solution ◽

Growth Regulators ◽

Root Formation ◽

Cultivated Plants ◽

Treat Ment ◽

Presowing Treatment ◽

Murashige Skoog ◽

Half Strength ◽

Semi Solid

Aim. The aim of the study was to choose conditions for rooting improvement of in vitro cultivated plants of some species of Carlina L. genus. Methods. For receiving and rooting of aseptic sprouts, seeds of Carlina acaulis L., Carlina cirsioides Klok and Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl were subjected to presowing treatment with gibberellic acid solution (GA3) or indolebyturic acid solution (IBA). Sterilized seeds were planted in sterile Petri dishes on semi-solid Murashige, Skoog nutrient medium with half-strength concentrations of macro- and microsalts without growth regulators. Results. It was found that with the seed soaking of C. acaulis, C. cirsioides and C. onopordifolia in GA3 solution the percentage of root formation amounted to 33.3 %, 33.3 % and 22.2 % respectively. Presowing treat-ment of carlina seeds in IBA solution with concentration of 1000 mg for 2–4 hours before sterilization gave a positive effect: the percentage of root formation for C. acaulis, C. cirsioides and C. onorordifolia was 2.4–4.5 times higher compared to the treatment with GA3 solution. Conclusions. To form the root system of carlina plants it is effective to soak the seeds in the solution of IBA. Thus we were able to increase the percentage of rooting of C. sirsioides and C. onorordifolia plants to 100 %, C. acaulis plants – up to 80 % and avoid sprouts’ injury and changes in the concentra-tions of the IBA, which may occur during sterilization at high temperatures by using non-sterile solution of growth regulators. Keywords: Carlina àcaulis L., Carlina cirsioides Klok, Carlina onopordifolia Besser ex Szafer, Kulcz. et Pawl, in vitro, sprouts rooting.

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