Determination of the Antioxidant Capacity of JiangHuang JiangZhi Capsule with DPPH Assay in Vitro

2011 ◽  
Vol 236-238 ◽  
pp. 913-916
Author(s):  
Sheng Zhang ◽  
Xiang Zhou Li ◽  
Chun Tao Kuang
Keyword(s):  
Free Radicals ◽  
Antioxidant Capacity ◽  
Antioxidant Effect ◽  
Assay Method ◽  
Dpph Assay

To investigate the antioxidant effect in vitro of JiangHuang JiangZhi capsule, different concentrations of content of capsule were determinated by DPPH assay method. Results showed Jianghuang Jiangzhi capsule had antioxidant capacity on eliminating DPPH free radicals.

Antioxidant Activity of Polysaccharide from Camellia Cake against ABTS and DPPH Free Radicals

2012 ◽  
Vol 550-553 ◽  
pp. 1545-1549 ◽  
Author(s):  
Sheng Zhang ◽  
Xiang Zhou Li ◽  
Zhi Ping Wu ◽  
Chun Tao Kuang
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Antioxidant Effect ◽  
Assay Method ◽  
Dependent Manner ◽  
Concentration Dependent Manner

The ABTS, and DPPH assays were used for determining to investigate the antioxidant effect in vitro of Polysaccharide from Camellia Cake. Different concentrations of the sample were determinated by assay method. Results showed Polysaccharide from Camellia Cake had an appreciable antioxidant effect in a concentration-dependent manner. The EC50 values against DPPH and ABTS free radicals were 0.106 mg/ml and 1.05mg/ml respectively.

scholarly journals Stimulation of phenolic compounds production in the in vitro cultivated Polyscias filicifolia Bailey shoots and evaluation of the antioxidant and cytotoxic potential of plant extracts

2018 ◽  
Vol 87 (2) ◽  
Author(s):  
Anita Agnieszka Śliwińska ◽  
Katarzyna Sykłowska-Baranek ◽  
Anita Kośmider ◽  
Sebastian Granica ◽  
Karolina Miszczak ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Cytotoxic Activity ◽  
Phenolic Compound ◽  
Plant Extracts ◽  
Detrimental Effect ◽  
A549 Cells ◽  
Ferulic Acids

<p>In this study, an efficient method to enhance phenolic compound production in the in vitro cultured shoots of <em>Polyscias filicifolia</em> was developed. The phenolic compound content in <em>P. filicifolia</em> has not yet been reported. Shoots were treated with methyl jasmonate (JM) or salicylic acid (SA) at doses of 50, 100, or 200 µM. HPLC-UV-VIS and LC-MS techniques were used for the determination of chlorogenic, caffeic, and ferulic acids. The total phenolics and flavonoids were quantified, and the antioxidant capacity of plant extracts was determined using DPPH and ABTS methods. Finally, the cytotoxic activity of <em>P. filicifolia</em> extracts in normal (HaCaT) and cancer (A549) cells was investigated. Further, the effect of the extracts on cisplatin cytotoxicity was assessed.</p><p>The elicitors significantly enhanced phenolic production compared to that in untreated shoots and leaves of intact plants. Chlorogenic acid was the most abundant compound with the highest yield of 5.03 ±0.25 mg/g DW after treatment with 50 µM SA. The total flavonoid and phenolic content was significantly and dose-dependently influenced by JM. The highest antioxidant capacity was noted in extracts derived from shoots grown on media supplemented with 50 µM SA and 200 µM JM; these doses were used for further cytotoxic activity investigations. The extracts from JM or SA treatments reduced cancer cell viability and increased their mortality, whereas the extract from JM treatment exhibited protective effect on normal cells. Moreover, the comparison of cytotoxic properties of plant extracts and cisplatin indicated that plant phenolic compounds in combination with anticancer drugs could reduce the detrimental effect of the latter on human cells.</p>

Methodological Aspects about Determination of Phenolic Compounds and In Vitro Evaluation of Antioxidant Capacity in the Honey: A Review

2009 ◽  
Vol 5 (4) ◽  
pp. 293-302 ◽  
Author(s):  
Jose Alvarez-Suarez ◽  
Sara Tulipani ◽  
Stefania Romandini ◽  
Alexis Vidal ◽  
Maurizio Battino
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
In Vitro Evaluation ◽  
Methodological Aspects

2,5-disubstituted-4-thiazolidinones: Synthesis, anti-inflammatory, free radical scavenging potentials and structural insights through molecular docking

2021 ◽  
Vol 18 ◽  
Author(s):  
Jagseer Singh ◽  
Pooja A Chawla ◽  
Rohit Bhatia ◽  
Shamsher Singh
Keyword(s):  
Free Radicals ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Assay Method ◽  
Acidic Group ◽  
Active Compounds ◽  
Cox 2 ◽  
Anti Inflammatory

: The present work reports synthesis and screening of fifteen 2,5-disubstituted-4-thiazolidinones with different substitutions of varied arylidene groups at imino. The structures of the compounds were confirmed by spectral characterization. The compounds were subjected to in vivo anti-inflammatory and in vitro antioxidant activities. The derivatives possessed remarkable activities quite close to standard drugs used. Unlike conventional non-selective NSAIDs, the synthesized compounds did not contain any acidic group, thereby ensuring a complete cure from ulcers. To further substantiate the claim for safer derivatives, the active compounds were docked against the cyclooxygenase (COX)-2 enzyme. It was found that 4-fluorophenylimino substituent at 2- position and 3-nitro moiety on a 5-benzylidene nucleus of the 4-thiazolidinone derivative fitted in the COX-2 binding pocket. The compounds exhibited remarkable activity in scavenging free radicals, as depicted by the DPPH assay method. The structure-activity relationship was also established in the present work with respect to the nature and position of the substituents. The active compounds were evaluated for drug-like nature under Lipinski’s rule of five, and the toxicity behaviour of active compounds was predicted using ADMETlab software. The compounds have the potential to target degenerative disorders associated with inflammation and the generation of free radicals.

scholarly journals Determination of in Vitro Antioxidant Enzyme Capacity and Oxidative Stress Levels in Mazı Meşesi (Quercus infectoria)

2021 ◽  
Vol 9 (4) ◽  
pp. 814-817
Author(s):  
İlter Demirhan ◽  
Büşra Çitil ◽  
Mehmet Özyurt ◽  
Meltem Güngör ◽  
Erkan Öner ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Antioxidant Capacity ◽  
Antioxidant Enzyme ◽  
Antioxidant Enzyme Activities ◽  
Spectrophotometric Methods ◽  
Significant Difference ◽  
Quercus Infectoria

South East Anatolia Region has a large genetic plant diversity due to its physical and different climatic charesteristics. These plants are potential sources of antioxidants that prevent oxidative stress caused by oxygen and photons. In recent years, it has become important to study the antioxidant capacity of many molecules found naturally in foods and biological systems. The reason for this is that it is believed that when the consumption of food rich in antioxidants is increased, the risk of developing different degenerative diseases will be reduced. In this study, it was aimed to measure the antoxidant capacity of Quercus infectoria, G.olivier gal seeds grown in Southeastern Anatolia. Q. infectoria gal seeds from Sanlıurfa province were used in our study. Q. infectoria gal seeds were extracted with water, ethanol and methanol and then antioxidant enzyme activities (catalase and superoxide dismutase) and malondialdehyde levels, which are indicators of oxidative stress were determined by spectrophotometric methods. It was found that the antioxidant capacity (catalase and superoxide dismutase activities) of extracts obtained from ethanol and methanol were higher and their malondialdehyde levels were statistically lower than those obtained from water. However, it was determined that there was no statistically significant difference between the antioxidant capacity and malondialdehyde levels of the extracts obtained from methanol compared to the extracts obtained from ethanol. It has been concluded that Q. infectoria gal seed has a effective antioxidant effect. In addition, it was observed that extracts obtained from ethanol and methanol have higher antioxidant capacity than extracts obtained from water.

scholarly journals The Influence of Lactic Acid Bacteria from Xinjiang Traditional Fermented Yogurt on Antioxidation of Geniposide in vitro

2020 ◽  
Vol 185 ◽  
pp. 04005
Author(s):  
Rui Xue ◽  
Mei Wang ◽  
Dengqiu Liu ◽  
Xin Zhao ◽  
Bihui Liu
Keyword(s):  
Lactic Acid ◽  
Free Radicals ◽  
Lactic Acid Bacteria ◽  
Sulfonic Acid ◽  
Potential Method ◽  
Antioxidant Effect ◽  
The Other ◽  
Scavenging Activity ◽  
Hydroxyl Free Radicals

To investigate the influence of lactic acid bacteria on the antioxidant effect of geniposide in vitro, seven strains of lactic acid bacteria were isolated from natural fermented yoghurt from Xinjiang, China. Lactic acid bacteria, geniposide and their mixtures were used separately to evaluate the antioxidant effect through detecting the scavenging activity of 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH), Hydroxyl free radicals and 2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS). The results showed that, compared with the group that geniposide separately used, the strains numbered x70-1, x70-2, x70-3, x70-4 and x70-5, combined with geniposide had more remarkable antioxidant effect, while the other two strains (numbered x43-2 and x43-3)combined with geniposide showed low oxidation resistance. These results may provide a potential method to promote antioxidant effect of geniposide.

Study and Evaluation of the release of Flavonoids extracted from various Eggplant cones from Semisolid Pharmaceutical Forms (In vitro)

2021 ◽  
pp. 5411-5417
Author(s):  
Jameela Hasian
Keyword(s):  
Free Radicals ◽  
Antioxidant Capacity ◽  
Ethanolic Extract ◽  
Zero Order ◽  
Main Components ◽  
Organic Solutions ◽  
Release Model ◽  
Kinetics Of ◽  
Active Substances

Various semisolid pharmaceutical forms have been prepared from eggplant calyx extract which rich in flavonoids and antioxidants. These ingredients were titrated in the prepared extracts, and then incorporated into the prepared pharmaceutical forms. Eggplant calyxes were used for three varieties cultivated in Syria. The flavonoids were extracted from them by using several aqueous and organic solutions. Three types of extracts were prepared for each type of eggplant (aqueous, ethanolic 70%, methanolic). The ethanolic extract of black eggplant showed the highest content of flavonoids. The black eggplant extract contained the largest amount of flavonoids in the three types of extracts. The antioxidant capacity of the plant extracts was measured by measuring its ability to remove free radicals of the fixed root DPPH, and the ethanolic extract of the black eggplant showed the best antioxidant capacity. Three topical pharmaceutical formulas were prepared of the ethanolic extract 70% for the rectangular black eggplant calyx, and the kinetics of the release of the main components from them in vitro was studied using a Franz cell, within four hours to determine the release model for each formulation in order to compare the formulas and select the best one. The cream of the (o / w) pattern gave the best release of the active substances because it released the highest percentage (92%) after two hours and the release pattern was of the zero order, while the rest of the formulas had the highest percentage of release after four hours with some deficiency of the active substances in them.

scholarly journals Antigenotoxic Effect of Chamomilla recutita (L.) Rauschert Essential Oil in Mouse Spermatogonial Cells, and Determination of Its Antioxidant Capacity in Vitro

2010 ◽  
Vol 11 (10) ◽  
pp. 3793-3802 ◽  
Author(s):  
Alejandra Hernández-Ceruelos ◽  
Eduardo Madrigal-Santillán ◽  
José Antonio Morales-González ◽  
Germán Chamorro-Cevallos ◽  
Martha Cassani-Galindo ◽  
...  
Keyword(s):  
Essential Oil ◽  
Antioxidant Capacity ◽  
Antigenotoxic Effect
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