Preliminary assessment of Rosmarinus officinalis toxicity on male Wistar rats' organs and reproductive system

Abstract The consumption of fructose has increased in children and adolescents and is partially responsible for the high incidence of metabolic diseases. The lifestyle during postnatal development can result in altered metabolic programming, thereby impairing the reproductive system and fertility during adulthood. Therefore, the aim of this study was to evaluate the effect of a high-fructose diet in the male reproductive system of pubertal and adult rats. Male Wistar rats (30 d old) were assigned to four different groups: Fr30, which received fructose (20%) in water for 30 d and were euthanized at postnatal day (PND) 60; Re-Fr30, which received fructose (20%) for 30 d and were euthanized at PND 120; and two control groups C30 and Re-C30, which received water ad libitum and were euthanized at PND 60 and 120, respectively. Fructose induced an increase in abnormal seminiferous tubules with epithelial vacuoles, degeneration, and immature cells in the lumen. Moreover, Fr30 rats showed altered spermatogenesis and daily sperm production (DSP), as well as increased serum testosterone concentrations. After discontinuing high-fructose consumption, DSP and sperm number decreased significantly. We observed tissue remodeling in the epididymis, with a reduction in stromal and epithelial compartments that might have influenced sperm motility. Therefore, we concluded that fructose intake in peripubertal rats led to changes in the reproductive system observed both during puberty and adulthood.

Download Full-text

Assessment of sperm production and reproductive organs of Wistar rats to long-term exposure of Caesalpinia ferrea

Anais da Academia Brasileira de Ciências ◽

10.1590/s0001-37652010000400013 ◽

2010 ◽

Vol 82 (4) ◽

pp. 907-914 ◽

Cited By ~ 6

Author(s):

Leda M.F. Lucinda ◽

Camila B. Rocha ◽

Maycon M. Reboredo ◽

Vinícius C. Faria ◽

Rita C.S. Sá

Keyword(s):

Reproductive System ◽

Wistar Rats ◽

Wistar Rat ◽

Reproductive Organs ◽

The Body ◽

Male Rats ◽

Sperm Production ◽

Male Wistar Rats ◽

The Right

Caesalpinia ferrea Mart (Leguminosae) is a medicinal plant used to treat diabetes, among other therapeutic properties, but which is also reported to have hepatotoxic effects. Although it contains substances such as flavonoids and coumarin, which are known to have antifertility activity, no studies have apparently been conducted to evaluate the potential adverse side effects of this plant on the function of the reproductive system after a chronic treatment. Therefore, this investigation was carried out to evaluate the effect and safety of the long-term exposure to C. ferrea on male Wistar rats' vital organs, reproductive system and sperm production. Adult and immature male rats were treated with an aqueous extract of C. ferrea at a dose level of 300 mg/kg of body weight, administered during one or two spermatogenic cycles of this species. The reproductive and vital organs were analyzed, and sperm was collected from the epididymal secretion of the right epididymis cauda. The long-term administration of C. ferrea did not significantly alter the body, vital and reproductive organs weights. Gamete production was not affected either. The chronic assessment of C. ferrea suggests that this plant does not affect the normal functioning of the Wistar rat reproductive system.

Download Full-text

Comparative Modulation of the Reproductive System by Ethanolleaf Extracts of Asystasia Gangetica Andanthocleista Vogelli in Male Wistar Rats

European Journal of Medical and Health Sciences ◽

10.24018/ejmed.2020.2.3.287 ◽

2020 ◽

Vol 2 (3) ◽

Author(s):

Simeon I. Egba ◽

C. O. Okonkwo ◽

H. C. Omeoga ◽

I. E. Ekong

Keyword(s):

Luteinizing Hormone ◽

Reproductive System ◽

Wistar Rats ◽

Follicle Stimulating Hormone ◽

Reproductive Hormones ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Albino Rats ◽

Male Wistar Rats ◽

Stimulating Hormone

A number of medicinal plants have been found to influence the level of reproductive hormones and thus affect fertility in the overall. This work evaluated the effects of ethanol leaf extracts of A. gangetica and A. vogelli respectively on some reproductive system parameters in male Wistar rats. A total of sixteen (16) male albino rats were grouped into four (four rats in each group): group A served as normal control, group B received Immunace (Vitabiotics) group C and D received 400 mg/kg body weight of A. gangetica and A. vogelli extracts respectively. Extracts were administered orally to rats for 21 days, after which they were sacrificed by cervical dislocations and blood samples drawn by cardiac puncture. The effect of the extracts on testosterone, follicle stimulating hormone, luteinizing hormone and semen analysis of the test rats were determined using enzyme linked immunosorbent assay (ELISA) and standard techniques. Data collected were analyzed using Graph pad prism V6 and p values < 0.05 were adopted as significant. There was no significant (p>0.05) change in testosterone, FSH and luteinizing hormones in the group administered A. gangetica plant extract relative to the control. There was also no visible difference in the testes weight and sperm morphology relative to the control group. In contrast, administration of A. vogelli extract caused significant (p<0.05) decrease in testosterone and follicle stimulating hormone concentrations from: 1.12 ± 0.20 to 0.89 ± 0.05 and 1.41 ± 0.07 to 1.35 ± 0.12 respectively relative to the control group. While significant (p<0.05) decrease in luteinizing hormone (1.71 ± 0.15) was observed relative to the standard drug group (1.76 ± 0.05). A significant (p<0.05) decrease in sperm count and testes weight was also observed in rats treated with A. vogelli extract relative to the normal control.The results suggest that, A. vogelli extract may cause decreased fertility in male albino rats and could be developed further into potent male contraceptives. A. gangetica on the other hand, had no effect on male reproductive hormones.

Download Full-text

Modulatory effects of kolaviron (Garcinia kola extract) on spermogram and reproductive system of adult male wistar rats in lead acetate induced toxiocity

Journal of Toxicology and Environmental Health Sciences ◽

10.5897/jtehs2013.0262 ◽

2013 ◽

Vol 5 (7) ◽

pp. 121-130 ◽

Cited By ~ 3

Author(s):

Ayoola Isaac

Keyword(s):

Adult Male ◽

Reproductive System ◽

Lead Acetate ◽

Wistar Rats ◽

Garcinia Kola ◽

Male Wistar Rats

Download Full-text

Preliminary assessment of potential toxicity of methylated soybean protein and methylated β-lactoglobulin in male Wistar rats

Food and Chemical Toxicology ◽

10.1016/j.fct.2013.06.026 ◽

2013 ◽

Vol 59 ◽

pp. 618-625 ◽

Cited By ~ 8

Author(s):

Mahmoud Sitohy ◽

Ali Osman ◽

Ahmed Gharib ◽

Jean-Marc Chobert ◽

Thomas Haertlé

Keyword(s):

Wistar Rats ◽

Soybean Protein ◽

Preliminary Assessment ◽

Potential Toxicity ◽

Male Wistar Rats ◽

Β Lactoglobulin

Download Full-text

Effect of an Aqueous Extract of Alchornea Cordifolia (Euphorbiacea) Leaves on Sperm Parameters and Reproductive Function of Male Wistar Rats

European Scientific Journal ESJ ◽

10.19044/esj.2021.v17n17p236 ◽

2021 ◽

Vol 17 (17) ◽

Author(s):

Kone Allassane ◽

Gnahoue Goueh ◽

Diabate Daouda ◽

Kouakou Koffi

Keyword(s):

Traditional Medicine ◽

Reproductive System ◽

Wistar Rats ◽

Sperm Quality ◽

Sperm Count ◽

Sperm Concentration ◽

Reproductive Function ◽

Positive Effects ◽

Male Wistar Rats ◽

Reproductive Male

Alchornea cordifolia (euphorbiaceae) is a tree whose leaves are used in traditional medicine to cure several health problems. Little information, however, exist on the effects of this herb on the male reproductive system. In order to assess the effect of Alchornea cordifolia on reproductive male parameters and testosterone production, twenty-four male wistar rats divided into four groups of six rats were used. The aqualus extract of Alchornea cordifolia was administered at a dose of 100 mg/kg of bw, 200 mg/kg of bw, and 400 mg/kg of bw per day, orally for 60 days. A significant increase in androgen-dependent organs, testosterone quantity, mobility, and sperm concentration was observed at doses of 200 mg/kg of bw and 400 mg/kg of bw. Only the unwinger sperm count significantly increased at the 100 mg/kg of bw dose. These results revealed that the aqualius extract of the leaves of Alchornea cordifolia has the potential to improve sperm quality and have positive effects on the reproductive system.

Download Full-text

Toxicological screening of Mikania glomerata Spreng., Asteraceae, extract in male Wistar rats reproductive system, sperm production and testosterone level after chronic treatment

Revista Brasileira de Farmacognosia ◽

10.1590/s0102-695x2010005000007 ◽

2010 ◽

Vol 20 (5) ◽

pp. 718-728 ◽

Cited By ~ 1

Author(s):

Rita de Cássia da Silveira e Sá ◽

Magda N. Leite ◽

Reinaldo N. de Almeida

Keyword(s):

Testosterone Level ◽

Reproductive System ◽

Wistar Rats ◽

Chronic Treatment ◽

Toxicological Screening ◽

Sperm Production ◽

Male Wistar Rats ◽

Mikania Glomerata

Download Full-text

EFFECTS OF ROSEMARY (ROSMARINUS OFFICINALIS L.) LEAF EXTRACT ON ANGULAR CHEILITIS INDUCED BY STAPHYLOCOCCUS AUREUS AND CANDIDA ALBICANS IN MALE WISTAR RATS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2018.v10s1.39 ◽

2018 ◽

Vol 10 (1) ◽

pp. 178 ◽

Cited By ~ 1

Author(s):

Sumintarti . ◽

Nurul Fatimasari S ◽

Andi S Hajrah-yusuf ◽

Muhammad Ruslin

Keyword(s):

Staphylococcus Aureus ◽

Candida Albicans ◽

Wistar Rats ◽

Leaf Extract ◽

Rosmarinus Officinalis ◽

Fourth Group ◽

The Third ◽

Male Wistar Rats ◽

Rosmarinus Officinalis L ◽

Wound Length

Objective: The study aimed to determine the effect of rosemary (Rosmarinus officinalis L.) leaf extract on healing angular cheilitis induced byStaphylococcus aureus and Candida albicans in male Wistar rats.Methods: We assessed 24 male Wistar rats that were divided into four groups: The first group was induced by S. aureus and given rosemary leafextract, the second group was induced by S. aureus and given gentamicin, the third group was induced by C. albicans and given rosemary leaf extract,and the fourth group was induced by C. albicans and given miconazole. All rats in each group were treated, and wound length and erythema wereobserved for 7 days.Results: The average wound length on the 7th day was 0.00±0.0 mm in the first group, 0.29±0.4 mm in the second group, 0.00±0.0 mm in the thirdgroup, and 0.25±0.2 mm in the fourth group. Statistical analysis demonstrated significant differences in wound length for each treatment group.Conclusion: Rosemary leaf extract has a healing effect on angular cheilitis induced by S. aureus and C. albicans in male Wistar rats.

Download Full-text