scholarly journals Evaluation of cleaning and disinfection performance of automatic washer disinfectors machines in programs presenting different cycle times and temperatures

2006 ◽  
Vol 14 (5) ◽  
pp. 735-741 ◽  
Author(s):  
Maria do Carmo Noronha Cominato Bergo
Keyword(s):  
Scientific Research ◽  
Soil Test ◽  
High Efficacy ◽  
Cycle Times ◽  
Test Kit ◽  
Standard Operating ◽  
Before And After ◽  
Cleaning And Disinfection ◽  
Cleaning Efficacy ◽  
Thermal Disinfection

Thermal washer-disinfectors represent a technology that brought about great advantages such as, establishment of protocols, standard operating procedures, reduction in occupational risk of a biological and environmental nature. The efficacy of the cleaning and disinfection obtained by automatic washer disinfectors machines in running programs with different times and temperatures determined by the different official agencies was validated according to recommendations from ISO Standards 15883-1/1999 and HTM2030 (NHS Estates, 1997) for the determining of the Minimum Lethality and DAL both theoretically and through the use with thermocouples. In order to determine the cleaning efficacy, the Soil Test, Biotrace Pro-tect and the Protein Test Kit were used. The procedure to verify the CFU count of viable microorganisms was performed before and after the thermal disinfection. This article shows that the results are in compliance with the ISO and HTM Standards. The validation steps confirmed the high efficacy level of the Medical Washer-Disinfectors. This protocol enabled the evaluation of the procedure based on evidence supported by scientific research, aiming at the support of the Supply Center multi-professional personnel with information and the possibility of developing further research.

scholarly journals Efficacy of Three Commercial Disinfectants in Reducing Microbial Surfaces’ Contaminations of Pharmaceuticals Hospital Facilities

2021 ◽  
Vol 18 (2) ◽  
pp. 779
Author(s):  
Giuseppina Di Martino ◽  
Salvatore Pasqua ◽  
Bruno Douradinha ◽  
Francesco Monaco ◽  
Chiara Di Bartolo ◽  
...  
Keyword(s):  
United States Pharmacopeia ◽  
Log10 Reduction ◽  
Colony Forming Units ◽  
Before And After ◽  
Disinfection Procedure ◽  
Cleaning And Disinfection ◽  
States Pharmacopeia ◽  
Representative Samples ◽  
Correct Implementation ◽  
Reduction Index

To evaluate and validate the efficacy of disinfectants used in our cleaning procedure, in order to reduce pharmaceutical hospital surfaces’ contaminations, we tested the action of three commercial disinfectants on small representative samples of the surfaces present in our hospital cleanrooms. These samples (or coupons) were contaminated with selected microorganisms for the validation of the disinfectants. The coupons were sampled before and after disinfection and the microbial load was assessed to calculate the Log10 reduction index. Subsequently, we developed and validated a disinfection procedure on real surfaces inside the cleanrooms intentionally contaminated with microorganisms, using approximately 107–108 total colony forming units per coupon. Our results showed a bactericidal, fungicidal, and sporicidal efficacy coherent to the acceptance criteria suggested by United States Pharmacopeia 35 <1072>. The correct implementation of our cleaning and disinfection procedure, respecting stipulated concentrations and contact times, led to a reduction of at least 6 Log10 for all microorganisms used. The proposed disinfection procedure reduced the pharmaceutical hospital surfaces’ contaminations, limited the propagation of microorganisms in points adjacent to the disinfected area, and ensured high disinfection and safety levels for operators, patients, and treated surfaces.

scholarly journals EVALUATION OF THREE SURFACE FRICTION TECHNIQUES FOR THE REMOVAL OF ORGANIC MATTER

2015 ◽  
Vol 24 (4) ◽  
pp. 1061-1070 ◽  
Author(s):  
Marcelo Alessandro Rigotti ◽  
Adriano Menis Ferreira ◽  
Mara Corrêa Lelles Nogueira ◽  
Margarete Teresa Gottardo de Almeida ◽  
Odanir Garcia Guerra ◽  
...  
Keyword(s):  
Organic Matter ◽  
Adenosine Triphosphate ◽  
Organic Material ◽  
Surface Friction ◽  
Disinfection Process ◽  
Significant Difference ◽  
Before And After ◽  
Quantitative Descriptive ◽  
The One ◽  
Cleaning And Disinfection

ABSTRACT The objective of this study was to assess the effectiveness of three surface friction techniques for the removal of organic material. A quantitative, descriptive and exploratory study was developed to evaluate the presence or not of organic material before and after the cleaning and disinfection process of surfaces of bedside tables of patients hospitalized at an Intensive Care Unit. Three friction techniques were executed in the one-way, two-way and centrifugal sense, individually, three times on each table, during alternate weeks. For each patient unit and friction technique, a single table and three sides of cloth were used, moistened with 70% (w/v) alcohol. The organic matter was detected through the presence of adenosine triphosphate by bioluminescence, using 3M(tm) Clean-Trace(tm) ATP Systems. For each technique, 13 samples were collected before and 13 after the cleaning/disinfection process, totaling 78 samples of adenosine triphosphate by bioluminescence. No statistically significant difference was found among the removal techniques of organic matter. This study demonstrated that none of the three surface friction methods was better than the other to remove organic matter. Nevertheless, further research is needed in which other cleaning/disinfection indicators and surfaces are considered.

scholarly journals Effectiveness of Surface Cleaning and Disinfection in a Brazilian Healthcare Facility

2018 ◽  
Vol 12 (1) ◽  
pp. 36-44 ◽  
Author(s):  
Aires G. Santos-Junior ◽  
Adriano M. Ferreira ◽  
Oleci P. Frota ◽  
Marcelo A. Rigotti ◽  
Larissa da S. Barcelos ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Adenosine Triphosphate ◽  
Healthcare Facility ◽  
Surface Cleaning ◽  
Point Of View ◽  
Colony Count ◽  
Before And After ◽  
Bioluminescence Assay ◽  
Cleaning And Disinfection ◽  
Hospital Cleaning

Background: Failures in the processes of cleaning and disinfecting health service surfaces may result in the spread and transfer of pathogens that are often associated with healthcare-related infections and outbreaks. Aims: To assess the effectiveness of environmental surface cleaning and disinfection in a hospital clinic. Method: The study was conducted in a nursing ward with 45 beds. A total of 80 samples from five high-touch surfaces were evaluated before and after cleaning and disinfection, using the following methods: visual inspection, adenosine triphosphate bioluminescence assay, aerobic colony count, Staphylococcus aureus colony count, and evaluation of resistance to methicillin. The data analysis used nonparametric comparative and correlative tests to observe any differences in the pre- and post- cleaning and disinfection results for the surfaces assessed. Results: Effective cleaning and disinfection had a significant effect on only two surfaces when measured for the presence of adenosine triphosphate, the inner bathroom door handle (p=0.007) and the toilet bowl (p=0.01). When evaluated for Staphylococcus aureus colony count, the toilet flush handle also demonstrated a significant effect (p=0.04). Conclusion: The effectiveness of cleaning and disinfection of the surfaces tested was not satisfactory. An educational intervention is recommended for the cleaning and disinfection staff and the nursing team at the healthcare facility. Relevance to Clinical Practice: The data in the study revealed that daily hospital cleaning and disinfection in the sampled sites are not sufficient in medical and surgical wards. Hospital cleanliness must be reevaluated from the point of view of materials, such as an adequate supply of clean cloths, in addition to establishing more precise cleanliness protocols and accurate monitoring systems.

scholarly journals Single Blinded Study on the Feasibility of Decontaminating LA-MRSA in Pig Compartments under Routine Conditions

Antibiotics ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 141
Author(s):  
Iris Kobusch ◽  
Hannah Müller ◽  
Alexander Mellmann ◽  
Robin Köck ◽  
Marc Boelhauve
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Great Effort ◽  
Methicillin Resistant ◽  
Air Samples ◽  
Blinded Study ◽  
Before And After ◽  
Cleaning And Disinfection ◽  
Routine Cleaning ◽  
Pig Husbandry

In countries with intensive pig husbandry in stables, the prevalence of livestock-associated (LA) methicillin-resistant Staphylococcus aureus (MRSA) on such farms has remained high in the last few years or has also further increased. Simple measures to reduce the LA-MRSA among pigs have not yet been successfully implemented. Earlier publications showed a decontamination of LA-MRSA was only possible with great effort. The aim of this study is to determine the suitability of routine cleaning and disinfection (C&D) for adequate LA-MRSA decontamination. For this purpose, at least 115 locations in a piglet-rearing compartment were examined before and after cleaning and disinfection. The sample locations were stratified according to accessibility for pigs and the difficulty of cleaning. The cleaning work was carried out routinely by farm employees, who were not informed about the sampling (single blinded). While before cleaning and disinfection, 85% of the samples from the surfaces were LA-MRSA positive, while only 2% were positive thereafter. All LA-MRSA-positive samples after cleaning and disinfection were outside the animal area. Air samples also showed no LA-MRSA after cleaning and disinfection. Conclusion: In well-managed livestock farms, decontamination of the LA-MRSA barn is quite possible; after C&D no LA-MRSA was detectable at animal height.

scholarly journals Impact of cleaning and disinfection procedures on microbial ecology and Salmonella antimicrobial resistance in a pig slaughterhouse

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Arnaud Bridier ◽  
Patricia Le Grandois ◽  
Marie-Hélène Moreau ◽  
Charleyne Prénom ◽  
Alain Le Roux ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Microbial Ecology ◽  
Bacterial Diversity ◽  
Food Environments ◽  
Short Term ◽  
Short Term Study ◽  
Before And After ◽  
Associated Surfaces ◽  
Cleaning And Disinfection

Abstract To guarantee food safety, a better deciphering of ecology and adaptation strategies of bacterial pathogens such as Salmonella in food environments is crucial. The role of food processing conditions such as cleaning and disinfection procedures on antimicrobial resistance emergence should especially be investigated. In this work, the prevalence and antimicrobial resistance of Salmonella and the microbial ecology of associated surfaces communities were investigated in a pig slaughterhouse before and after cleaning and disinfection procedures. Salmonella were detected in 67% of samples and isolates characterization revealed the presence of 15 PFGE-patterns belonging to five serotypes: S.4,5,12:i:-, Rissen, Typhimurium, Infantis and Derby. Resistance to ampicillin, sulfamethoxazole, tetracycline and/or chloramphenicol was detected depending on serotypes. 16S rRNA-based bacterial diversity analyses showed that Salmonella surface associated communities were highly dominated by the Moraxellaceae family with a clear site-specific composition suggesting a persistent colonization of the pig slaughterhouse. Cleaning and disinfection procedures did not lead to a modification of Salmonella susceptibility to antimicrobials in this short-term study but they tended to significantly reduce bacterial diversity and favored some genera such as Rothia and Psychrobacter. Such data participate to the construction of a comprehensive view of Salmonella ecology and antimicrobial resistance emergence in food environments in relation with cleaning and disinfection procedures.

scholarly journals Observations on Salmonella contamination of commercial duck farms before and after cleaning and disinfection

2016 ◽  
Vol 46 (2) ◽  
pp. 131-137 ◽  
Author(s):  
Francesca Martelli ◽  
Rebecca J Gosling ◽  
Rebecca Callaby ◽  
Rob Davies
Keyword(s):  
Before And After ◽  
Cleaning And Disinfection

scholarly journals Gambaran Pelaksanaan Supervisi Kepala Ruang Terhadap Perawat Pelaksana Dalam Keselamatan Pasien

2019 ◽  
Vol 2 (1) ◽  
pp. 23
Author(s):  
Maria Hariyati Oktaviani ◽  
Muhamad Rofii
Keyword(s):  
Research Design ◽  
Nursing Care ◽  
Standard Operating Procedures ◽  
Assessment Instruments ◽  
Descriptive Research ◽  
Care Services ◽  
Standard Operating ◽  
Before And After ◽  
Quality Of Nursing

The implementation of supervising the head of a room in one hospital in Semarang has not been optimal due to the absence of a schedule, assessment instruments, guidance, documentation of supervision results, and standard operating procedures (SOP) supervision. Supervision activities are incidental in accordance with needs and have not been implemented in a structured and well-documented manner. The writing of this article aims to find out the description of the implementation of head supervision in a hospital in Semarang. This study uses descriptive research design. The subjects in this study were all heads of inpatient rooms. The object of this research is the implementation of the supervision of the head of the room according to the SOP and the results of documentation of the implementation of supervision. The instrument in this study used a draft sheet for evaluation of the superficial room leader evaluation. Shows that there is a change in the implementation of supervision based on the SOP before and after the dissemination of supervision is carried out to the head of the room. Documented supervision results can help the head of the room to see the extent of the ability of staff and can jointly improve capabilities, correct errors in improving the quality of nursing care services. The implementation of supervising the head of a room in one of the Semarang hospitals needs to be improved, especially in terms of post-supervision documentation, development of thematic supervision themes, and structured supervision scheduling.

scholarly journals Monitoring Oil Concentration In The Field

1999 ◽  
Vol 1999 (1) ◽  
pp. 1171-1173
Author(s):  
Patrick Lambert ◽  
B. Fieldhouse ◽  
Mervin F. Fingas ◽  
Mike Goldthorp
Keyword(s):  
Petroleum Hydrocarbons ◽  
Soil Test ◽  
Oil And Grease ◽  
Concentration Data ◽  
Sample Matrix ◽  
Test Kit ◽  
Field Samples ◽  
Oil Concentration ◽  
Ir Analysis ◽  
Petroleum Fuels

ABSTRACT The performance of three field kits was compared using prepared diesel fuel-in-sand samples and environmental field samples. The field kits were the EnviroGard Petroleum Fuels in Soil Test kit (EnviroGard, Millipore Canada, Mississauga, Ontario), PetroFlag Hydrocarbon Test Kit for Soil (Dexsil, Hamden, Connecticut), and a modified version of the method for oil and grease and petroleum hydrocarbons solvent extraction with infrared (IR) analysis (Buck Scientific, East Norwalk, Connecticut). The EnviroGard kit was affected by the sample matrix and was not capable of detecting the petroleum concentration. The Petroflag kit tended to generate results higher than the accepted concentration. The IR method was the most sensitive and produced values similar to the accepted concentration data.

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